Library Contained In Or Displayed By A Micro-organism (e.g., Bacteria, Animal Cell, Etc.) Or Library Contained In Or Displayed By A Vector (e.g., Plasmid, Etc.) Or Library Containing Only Micro-organisms Or Vectors Patents (Class 506/14)
  • Patent number: 11547101
    Abstract: A non-human animal model for neurodegenerative and/or inflammatory diseases is provided, which non-human animal comprises a disruption in a C9ORF72 locus. In particular, non-human animals described herein comprise a deletion of an entire coding sequence of a C9ORF72 locus. Methods of identifying therapeutic candidates that may be used to prevent, delay or treat one or more neurodegenerative (e.g., amyotrophic lateral sclerosis (ALS, also referred to as Lou Gehrig's disease) and frontotemporal dementia (FTD)), autoimmune and/or inflammatory diseases (e.g., SLE, glomerulonephritis) are also provided.
    Type: Grant
    Filed: March 27, 2019
    Date of Patent: January 10, 2023
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Amanda Atanasio, Burcin Ikiz, Guochun Gong, Michael L. Lacroix-Fralish, Ka-Man Venus Lai, David M. Valenzuela
  • Patent number: 11473133
    Abstract: Compositions, systems and methods for generating and using internal standard spike-in mixes including a combination of template spikes. Compositions, systems and methods described herein are directed to using the internal standard spike-in mixes to evaluate a set of workflow pipelines to perform differential abundance analyses on a sample containing variations of a target nucleic acid sequence of interest. Compositions, systems and methods described herein are directed to using the internal spike-in mixes to validate results obtained from differential abundance analyses performed on a sample containing variations of a target nucleic acid sequence of interest, where the variations may be of highly variable levels of relative abundance.
    Type: Grant
    Filed: September 24, 2019
    Date of Patent: October 18, 2022
    Assignee: Second Genome, Inc.
    Inventors: Cheryl-Emiliane T. Chow, Todd Z. DeSantis, Roberta L. Hannibal, Jayamary Divya Ravichandar, Nicole R. Narayan
  • Patent number: 11473080
    Abstract: A combined Kunkel mutagenesis and phage-display method for producing bivalent binding agents is provided.
    Type: Grant
    Filed: November 5, 2018
    Date of Patent: October 18, 2022
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Brian K. Kay, Kevin T. Gorman, Renhua Huang
  • Patent number: 11382962
    Abstract: Vaccine compositions including a yeast comprising an immunostimulatory polypeptide and optionally an antigenic polypeptide are provided herein. The immunostimulatory polypeptide and the antigenic polypeptide are expressed or displayed on the surface of the yeast vaccine composition. Methods of using the vaccine composition to vaccinate subjects are also provided.
    Type: Grant
    Filed: June 11, 2020
    Date of Patent: July 12, 2022
    Assignees: The Board of Trustees of the University of Arkansas, The Texas A&M University System
    Inventors: Olivia B. Faulkner, Lisa Bielke, Leona Nicole Calhoun, Luc Berghman, Billy Hargis
  • Patent number: 11268950
    Abstract: Disclosed are methods of assessing the ability of a candidate therapeutic agent to reverse, reduce or prevent renal injury by a potential toxic agent using a three-dimensional, engineered, bioprinted, biological renal tubule model. Also disclosed are methods of assessing the effect of an agent on renal function, the method comprising contacting the agent with a three-dimensional, engineered, bioprinted, biological renal tubule model. Also disclosed are models of renal disorder. In one embodiment, disclosed are models of renal fibrosis, comprising a three-dimensional, engineered, bioprinted, biological renal tubule model. Also disclosed are methods of making the model of renal disorder. In one embodiment disclosed are methods of making the model of renal fibrosis comprising contacting a three-dimensional, engineered, bioprinted, biological renal tubule model with an agent that is capable of inducing interstitial fibrotic tissue formation.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: March 8, 2022
    Assignee: Organovo, Inc.
    Inventors: Shelby Marie King, Deborah Lynn Greene Nguyen, Sharon C. Presnell
  • Patent number: 11236135
    Abstract: In a first aspect, the present invention relates to a mutated Salmonella strain comprising mutations in flagellin II genes, like the fliF gene, in particular, in addition the aroA gene, the IpxR gene, the pagP gene, the pagL gene, the ydiV gene and optionally the eptA gene and further optionally, the arnT gene. In a further aspect, immunogenic compositions comprising said Salmonella strain are provided optionally together with a pharmaceutically accepted carrier, diluent or effluent. Moreover, a method for producing outer membrane vesicles of Salmonella is provided, said method comprises the steps of cultivating the Salmonella strain according to the present invention and isolating the outer membrane vesicles accordingly.
    Type: Grant
    Filed: April 26, 2018
    Date of Patent: February 1, 2022
    Assignee: HELMHOLTZ-ZENTRUM FUR INFEKTIONSFORSCHUNG GMBH
    Inventors: Marc Erhardt, Sebastian Felgner, Dino Kocijancic, Siegfried Weiss
  • Patent number: 11230575
    Abstract: Disclosed is a method of producing an antimicrobial peptide wherein an antimicrobial peptide gene is fused with a green fluorescent protein gene expressed insolubly in E. coli, followed by introduction into E. coli, expression, and removal of the green fluorescent protein to yield the antimicrobial peptide. This method is capable of producing the antimicrobial peptide with high yield in a simple and economical manner and is thus effective at providing native antibiotics that can replace conventional antibiotics in pharmaceutical and feed industries, requiring the development of antibiotics having a new mechanism of action that can eradicate resistant strains due to the proliferation of multiple-drug-resistant microorganisms. Furthermore, the use of an amino acid cleavage process through acid treatment, instead of using conventional cyanogen bromide, is cost-effective for the purification of a target protein from an insoluble protein, can decrease the risk of processing, and enables rapid processing.
    Type: Grant
    Filed: February 26, 2016
    Date of Patent: January 25, 2022
    Assignee: Konkuk University Industrial Cooperation Corp
    Inventor: Chan Kyu Park
  • Patent number: 11124551
    Abstract: Described herein is a novel, mitochondrial encoded, open reading frame, that leads to the production of a new mitochondrial peptide. Residing within the ND-Two subunit, a specific small nucleotide polymorphism disrupts expression of this mitochondrial peptide, and is correlated with an increase in obesity and diabetes, particularly in certain ethnic populations. In vitro administration of the peptide increases insulin secretion, decreases fat accumulation and improves glucose uptake in muscle cell. Antibodies generated against the peptide can be used for detecting peptide deficiency, in addition to SNP detection, supporting diagnostic approaches. In vivo studies further revealed that administration of the peptide improves glucose tolerance, thereby providing a new therapeutic avenue for a novel diabetes therapy and decreases bodyweight, thus serving as a novel obesity therapy. Generation of synthetic analogs further enhance or abrogated activity relative to the natural peptide.
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: September 21, 2021
    Assignee: UNIVERSITY OF SOUTHERN CALIFORNIA
    Inventors: Pinchas Cohen, Kelvin Yen
  • Patent number: 11065208
    Abstract: Disclosed are compositions and methods for the co-delivery of ribonucleic acids and interferon binding proteins. Compositions include mineral coated microparticles having a mineral layer, a ribonucleic acid, and an interferon binding protein. Ribonucleic acids and interferon binding proteins can be adsorbed to the mineral layer, can be incorporated into the mineral layer, and combinations thereof. Also disclosed are methods for co-delivery of ribonucleic acids and interferon binding proteins and methods for treating inflammatory diseases using mineral coated microparticles having a mineral layer to provide co-delivery of ribonucleic acids and interferon binding proteins.
    Type: Grant
    Filed: July 5, 2018
    Date of Patent: July 20, 2021
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: William L. Murphy, Andrew Salim Khalil, Xiaohua Yu
  • Patent number: 10968496
    Abstract: The invention provides engineered Vibrio sp. organisms that comprise a genetic modification to either or both of the lpxL and/or lpxM genes. The organisms score substantially lower in an in vitro endotoxin assay versus the unmodified or wild type organism. The organisms preserve substantially the growth rate of the corresponding unmodified organisms. The organisms can also have an exogenous nucleic acid cloned in the organism, or an exogenous nucleic acid encoding a protein, polypeptide, or peptide expressed by the organism, and optionally secreted from the organism.
    Type: Grant
    Filed: October 8, 2018
    Date of Patent: April 6, 2021
    Assignee: Codex DNA, Inc.
    Inventors: Matthew T Weinstock, Daniel G. Gibson, Daniel Strimling
  • Patent number: 10858395
    Abstract: The present disclosure is directed to providing a new skin-penetrating peptide, a fusion product with a biologically active substance bound using the same, a cosmetic composition containing the same and a pharmaceutical composition for external application to skin containing the same. The skin-penetrating peptide of the present disclosure is less likely to cause an immune response as compared to existing skin-penetrating peptides and exhibits remarkably improved skin permeability. Therefore, the biologically active substance can be effectively delivered through the skin, particularly through the stratum corneum.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: December 8, 2020
    Assignee: IUCF-HYU (INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG UNIVERSITY)
    Inventors: Je-Min Choi, Wonju Kim, Ja-Hyun Koo, Jiyun Kim
  • Patent number: 10675757
    Abstract: A device for positioning a processing tool: a processing tool for processing the to-be-processed workpiece's surface while pressing the surface to be processed; a movement mechanism able to displace processing tool's distal end in a first direction orthogonal to the surface to be processed and/or a second direction parallel with the surface to be processed; a force sensor able to detect a force in the first and second direction applied to the processing tool's distal end pressed onto the surface to be processed; and a control device for executing a correction step for controlling the movement mechanism so the surface to be processed is pressed while the distal end's position of the processing tool is aligned with a processing reference position on the surface to be processed, and correcting the processing tool's position so that the force in the second direction is within a specific value or less.
    Type: Grant
    Filed: September 7, 2016
    Date of Patent: June 9, 2020
    Assignee: KAWASAKI JUKOGYO KABUSHIKI KAISHA
    Inventors: Tomohiro Kinoshita, Jun Fujimori, Yuhei Horiuchi, Kazunori Hara, Junichi Tamura, Satoshi Suzuki
  • Patent number: 10633652
    Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
    Type: Grant
    Filed: September 1, 2017
    Date of Patent: April 28, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Darren Roy Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
  • Patent number: 10577643
    Abstract: Presented herein are methods and compositions for enhancing specific enrichment of target sequences in a nucleic acid library. Off-target hybridization probes may be used to reduce binding and/or capture of off-target regions of a nucleic acid library in a targeted sequencing workflow. The off-target hybridization probes may be specific for locations known to generate off-target sequencing reads for a particular set of hybridization probes.
    Type: Grant
    Filed: October 5, 2016
    Date of Patent: March 3, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Li Teng, Chia-Ling Hsieh, Charles Lin, Han-Yu Chuang
  • Patent number: 10265411
    Abstract: The invention relates to (among other things) N-optionally substituted aryl-2-oligomer-3-alkoxypropionamides and compositions comprising the same. A compound of the invention, when administered by any of a number of administration routes, exhibits one or more advantages over corresponding compounds lacking the oligomer.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: April 23, 2019
    Assignee: Nektar Therapeutics
    Inventors: Jennifer Riggs-Sauthier, Wen Zhang
  • Patent number: 10196634
    Abstract: Methods of clonal analysis of functional genomic assays are provided. Aspects of the invention include transducing a population of target cells with a packaged viral effector library made up of a plurality of effector construct subsets, wherein each effector construct subset of the library includes a plurality of effector constructs having a common effector cassette linked to a distinct clonal barcode. Inclusion of distinct clonal barcodes in the effector construct subset allows for determination of the clonal representation of an effector construct subset in transduced target cells that exhibit a specific phenotype. Aspects of the invention further include compositions, e.g., libraries and components thereof, which find use in practicing the methods.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: February 5, 2019
    Assignee: Cellecta, Inc.
    Inventors: Alex Chenchik, Donato Tedesco, Mikhail Makhanov
  • Patent number: 10151762
    Abstract: The invention relates to agents and to pharmaceutical compositions for reducing the formation of amyloid and/or for promoting the disaggregation of amyloid proteins. The compositions may also be used to detect amyloid.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: December 11, 2018
    Assignee: Proclara Biosciences, Inc.
    Inventors: Rajaraman Krishnan, Richard Fisher
  • Patent number: 9464318
    Abstract: Methods, compositions, systems and kits to amplify or improve amplification of target-specific amplification products by reducing non-specific amplification products (e.g., primer-dimers) when amplifying multiple different nucleotide regions. The methods, compositions, systems and kits described herein may include, or include the use of, one or more resolvases that recognize and bind to and/or cut an aberrant DNA structure.
    Type: Grant
    Filed: February 11, 2016
    Date of Patent: October 11, 2016
    Assignee: Paragon Genomics, Inc.
    Inventor: Zhitong Liu
  • Patent number: 9453217
    Abstract: Antigen-specific immunoglobulin V-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all V-region sequences to be amplified (including those with extensive 5? end mutations) without loss of the original 5? V gene segment sequence. These libraries can be screened for antigen-specific V-regions using eukaryotic cells engineered to express the amplified V-region-encoding nucleic acids or using bacterial phage display techniques. In the latter, a second V-region library is made using a larger than conventional set of 5? V-region primers. The sequence errors introduced into the amplification products by this method are corrected using sequence information obtained in the products amplified by the V-region primers to screen the library created using the leader sequence primers.
    Type: Grant
    Filed: November 12, 2015
    Date of Patent: September 27, 2016
    Assignee: XBIOTECH, INC.
    Inventor: John Simard
  • Patent number: 9370564
    Abstract: The invention relates to a novel use of a Bordetella adenylcyclase toxin in the manufacturing of vectors for targeting in vivo a molecule of interest, specifically to CD11b expressing cells. The invention also relates to an immunogenic composition that primes immune responses, to pharmaceutical compositions and to a new vector for molecule delivery to CD11b expressing cells.
    Type: Grant
    Filed: June 27, 2011
    Date of Patent: June 21, 2016
    Assignees: INSTITUT PASTEUR, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Claude LeClerc, Pierre Guermonprez, Daniel Ladant, Nicole Guiso, Nadia Khelef, Cecile Bauche, Catherine Fayolle, Mohammed El-Azami El-Idrissi
  • Patent number: 9289766
    Abstract: The invention relates generally to methods and apparatus for conducting analyzes, particularly microfluidic devices for the detection of target analytes.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: March 22, 2016
    Assignee: Illumina, Inc.
    Inventors: Mark S. Chee, Todd A. Dickinson, Kevin Gunderson, Don O'Neil
  • Publication number: 20150148246
    Abstract: The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. Embodiments of the invention provide a system in which a bait complexed with a monovalent antibody fragment can be captured prior to secretion in a host cell by virtue of surface displaying an antibody light chain and utilizing the covalent interaction of this light chain with the heavy chain of an antibody molecule that is co-expressed in the same host. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of using the system for identifying antibodies that bind specifically to an antigen of interest.
    Type: Application
    Filed: May 6, 2013
    Publication date: May 28, 2015
    Inventors: Hussam Hisham Shaheen, Donxing Zha
  • Patent number: 9040258
    Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: May 26, 2015
    Assignee: Dyax Corp.
    Inventors: Simon E. Hufton, Hendricus Renerus Jacobus Mattheus Hoogenboom
  • Publication number: 20150141263
    Abstract: The invention relates to methods and compositions that enable the rapid generation of high-order combinations of genetic elements, and that provide a barcoded basis for rapid characterization of the specific combination of genetic elements encoded within a single cell or in a pooled population.
    Type: Application
    Filed: June 28, 2013
    Publication date: May 21, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: Timothy Kuan-Ta Lu, Allen Cheng
  • Publication number: 20150141284
    Abstract: The invention relates to the field of medicine. The invention provides methods for generating glatiramer-acetate-specific human T-cell lines, and assays that use these T-cell lines for demonstrating immunological identity between glatiramer acetate preparations. These assays allow sensitive and accurate comparison of glatiramer acetate preparations, and find utility as lot-release assays.
    Type: Application
    Filed: October 23, 2014
    Publication date: May 21, 2015
    Inventors: Jeffrey P. Smith, Peter E. Lipsky, Anne Lodge
  • Patent number: 9034601
    Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).
    Type: Grant
    Filed: July 9, 2013
    Date of Patent: May 19, 2015
    Assignee: Dyax Corp.
    Inventors: Simon E. Hufton, Hendricus Renerus Jacobus Mattheus Hoogenboom
  • Publication number: 20150133339
    Abstract: This invention provides a multiscale platform for coordinating behavior using synthetic biology. The platform reduces the impact of underlying noise, making outputs more coherent and reliable at the macroscopic level.
    Type: Application
    Filed: December 14, 2012
    Publication date: May 14, 2015
    Applicant: The Regents of the University of California
    Inventors: Arthur Prindle, Phillip Samayoa, Jeff Hasty
  • Publication number: 20150119264
    Abstract: The invention relates to novel variants that associate with Alzheimer's disease AD and their use in kits as a means for diagnosing AD; and also their use in nucleic acid molecules or cells/cell lines for identifying novel therapeutic, label of identification means.
    Type: Application
    Filed: April 14, 2014
    Publication date: April 30, 2015
    Applicant: University College Cardiff Consultants Limited
    Inventors: Julie Williams, Michael John Owen
  • Publication number: 20150119284
    Abstract: Disclosed is a microcapsule encoded depending on the kind of a target substance included therein. The encoded microcapsule has a hydrophilic liquid core including the target substance and a hydrophobic shell surrounding the liquid core. The encoded microcapsule includes graphical codes introduced on the surface of the shell.
    Type: Application
    Filed: October 28, 2013
    Publication date: April 30, 2015
    Applicant: SNU R&DB FOUNDATION
    Inventors: Sunghoon KWON, Younghoon SONG, Taehong KWON, Daewon LEE, Junhoi KIM
  • Patent number: 9012181
    Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).
    Type: Grant
    Filed: November 24, 2009
    Date of Patent: April 21, 2015
    Assignee: Dyax Corp.
    Inventors: Simon E. Hufton, Hendricus R. J. M. Hoogenboom
  • Patent number: 9005927
    Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).
    Type: Grant
    Filed: March 28, 2011
    Date of Patent: April 14, 2015
    Assignee: Dyax Corp.
    Inventors: Simon E. Hufton, Hendricus R. J. M. Hoogenboom
  • Patent number: 8987173
    Abstract: The present invention relates to the fields of microbiology, molecular biology and protein biochemistry. More particularly, it relates to compositions and methods for analyzing and altering (e.g., enhancing or inhibiting) protein folding and solubility.
    Type: Grant
    Filed: May 14, 2008
    Date of Patent: March 24, 2015
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Matthew P. DeLisa, Adam Charles Fisher
  • Publication number: 20150082466
    Abstract: The present invention relates to humanisation of antibodies in vivo. The invention provides non-human vertebrates, cells, populations and methods useful for humanising chimaeric antibodies in vivo. Using the present invention it is possible straightforwardly and rapidly to obtain antigen-specific antibodies that are fully human (ie, comprising human variable and constant regions) and have undergone recombination, junctional diversification, affinity maturation and isotype switching in vivo in a non-human vertebrate system. Furthermore, such antibodies are humanised (eg, totally human)—and selected—totally in vivo, and as such the present invention harnesses in vivo filtering for expressibility, affinity and biophysical characteristics in the context of the desired human variable and constant region pairings. This is avoids problems of down-grading antibody characteristics when humanising the constant region of chimaeric antibodies in vitro.
    Type: Application
    Filed: September 26, 2014
    Publication date: March 19, 2015
    Inventor: Jasper Clube
  • Publication number: 20150079038
    Abstract: Provided herein are methods of selective screening. In addition, various targeting proteins and sequences, as well as methods of their use, are also provided.
    Type: Application
    Filed: September 12, 2014
    Publication date: March 19, 2015
    Inventors: Benjamin E. Deverman, Paul H. Patterson
  • Publication number: 20150080234
    Abstract: The present disclosure provides technologies for achieving cell patterning, as well as patterned cell arrays achieved with such technologies. Provided apparatus and methods are useful in a variety of contexts and provide particular advantages with respect to assessing living cell arrays and/or their responsiveness to stimuli, including identifying and/or characterizing complex cellular responses.
    Type: Application
    Filed: September 16, 2014
    Publication date: March 19, 2015
    Inventors: John Christopher Love, Ayca Yalcin Ozkumur, Brittany Anne Thomas
  • Publication number: 20150065389
    Abstract: The present invention is drawn to the generation of micropatterns of biomolecules and cells on standard laboratory materials through selective ablation of a physisorbed biomolecule with oxygen plasma. In certain embodiments, oxygen plasma is able to ablate selectively physisorbed layers of biomolecules (e.g., type-I collagen, fibronectin, laminin, and Matrigel) along complex non-linear paths which are difficult or impossible to pattern using alternative methods. In addition, certain embodiments of the present invention relate to the micropatterning of multiple cell types on curved surfaces, multiwell plates, and flat bottom flasks. The invention also features kits for use with the subject methods.
    Type: Application
    Filed: March 26, 2014
    Publication date: March 5, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: David T. Eddington, Sangeeta N. BHATIA
  • Publication number: 20150065382
    Abstract: Methods for producing and identifying fragments of proteins, and more particularly to methods for generating and identifying soluble protein domains are disclosed based on a method for generating a library of nucleic acid fragments from nucleic acid encoding a desired polypeptide, and more especially a library of essentially, randomly sampled fragments of coding DNA sequence predominantly of defined size range and a method for selecting cloned gene fragments from the library that encode soluble protein domains.
    Type: Application
    Filed: September 5, 2014
    Publication date: March 5, 2015
    Inventors: Mark McAlister, Renos Savva, Laurence Pearl, Chrisostomos Prodromou, Paul C. Driscoll
  • Publication number: 20150057174
    Abstract: The present invention relates to a differential diagnostic method using flow cytometry, performed by means of differential fluorescent marking of biological agents, such as cells and pathogens of interest, with fluorescent substances. The diagnostic method generally consists in performing fluorescent marking of biological agents with gradual concentrations of fluorescent substances, and in analysing the reactivity profile of IgG1 to the biological agents. The present invention further relates to a diagnostic kit.
    Type: Application
    Filed: March 12, 2013
    Publication date: February 26, 2015
    Applicant: Fundacao Oswaldo Cruz
    Inventors: Olindo Assis Martins-Filho, Andrea Teixeira De Carvalho, Roberta Dias Rodrigues Rocha, Marileia Chaves Andrade, Danielle Marquete Vitelli Avelar, Stefan Michael Geiger, Fernanda Freire Campos Nunes, Marcio Sobreira Silva Araujo, Anna Barbara de Freitas Carneiro Proietti, Claudia Di Lorenzo Oliveira, Ester Cerdeira Sabino, Elenice Moreira Lemos
  • Publication number: 20150057184
    Abstract: Provided is a microarray platform for the culture of cells atop combinatorial matrix mixtures; enabling the study of differentiation in response to a multitude of microenvironments in parallel.
    Type: Application
    Filed: March 25, 2014
    Publication date: February 26, 2015
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Sangeeta N. BHATIA, Christopher FLAIM
  • Publication number: 20150057176
    Abstract: The present invention provides methods for early diagnosis of Alzheimer's disease and for determining the efficacy of a treatment for Alzheimer's disease in an Alzheimer's patient, i.e., monitoring Alzheimer's disease progression, utilizing cellular blood markers; as well as kits for carrying out these methods.
    Type: Application
    Filed: March 21, 2013
    Publication date: February 26, 2015
    Inventors: Michal Eisenbach-Schwartz, Ester Yoles
  • Publication number: 20150057186
    Abstract: The present invention relates to a filamentous phage display method wherein the polypeptides of interest displayed on the phage particle are cotranslationally translocated across the cytoplasmic membrane of Gram-negative bacteria based on the signal recognition particle pathway. This method is particularly suitable for polypeptides, which are known to be difficult to display on phages, and for proteins of cDNA libraries and other combinatorial libraries, in particular when derived from very fast folding, stable protein scaffolds. The invention further relates to phage or phagemid vectors useful in the method comprising a gene construct coding for a fusion polypeptide comprising the polypeptide to be displayed on the phage particle and an N-terminal signal sequence promoting cotranslational translocation.
    Type: Application
    Filed: August 26, 2014
    Publication date: February 26, 2015
    Inventors: Daniel STEINER, Patrik FORRER, Michael T. STUMPP, Andreas Plückthun
  • Patent number: 8962531
    Abstract: In one embodiment, a testing device includes a substrate and a plurality of spatially distinct fungal cultures disposed on the substrate.
    Type: Grant
    Filed: January 14, 2011
    Date of Patent: February 24, 2015
    Assignee: Board of Regents of the University of Texas System
    Inventors: Anand K. Ramasubramanian, Jose L. Lopez-Ribot, Anand Srinivasan, Priya Uppuluri
  • Publication number: 20150051108
    Abstract: The present invention relates to a method and a kit for monitoring an immune status after transplant. The kit for monitoring an immune status after transplant and the method for monitoring an immune status of an individual after transplant as provided in the present invention make it easy and accurate to determine an immune status in the individual after transplant and thus have an effect of reducing overuse of an immunosuppressive agent prescribed after transplant and also have an effect of conveniently managing an immune status of each patient.
    Type: Application
    Filed: November 7, 2014
    Publication date: February 19, 2015
    Inventors: Mi-La CHO, Chul-Woo YANG, Jong-Young CHOI, Joo-Yeon JHUN, Hee-Yeon KIM, Jae-Kyeong BYUN, Ye-Been YIM, Byung-Ha CHUNG, Kyoung-Woon KIM
  • Publication number: 20150052626
    Abstract: The invention provides cells or populations of cells, including non-human animals or non-human mammals having these cells, where the cells or populations of cells are stably tagged, uniquely identified and genetically barcoded by one or more detectable, e.g., fluorescent, proteins; and methods of making and using them. In alternative embodiments, the invention provides methods for tagging, uniquely identifying or genetically barcoding a cell, a population of cells, or a culture of cells by stably transferring, transfecting, transducing, infecting or implanting one or more nucleic acids encoding readable or detectable, e.g., fluorescent, moieties into the cells. In alternative embodiments, the nucleic acids are stably inserted into the cells such that the readable or detectable, e.g., fluorescent, genetic barcoding becomes a stable, heritable characteristic of the cell.
    Type: Application
    Filed: August 18, 2014
    Publication date: February 19, 2015
    Inventor: Roland WOLKOWICZ
  • Publication number: 20150045253
    Abstract: The invention relates to a method for determining the ability of a candidate antibody to keep a first cell and a second cell close to one another, said method comprising the following steps: bringing said candidate antibody in contact with (i) a first cell expressing, in the extracellular portion of its plasma membrane, a first protein that is known to be or is suspected of being recognized by the candidate antibody and (ii) a second cell expressing, in the extracellular portion of the plasma membrane, a second protein that is also known to be or suspected of being recognized by the candidate antibody, each of the proteins being labeled with a member of a pair of FRET partners, and measurement of the FRET signal and comparison with the signal measured in the absence of the candidate antibody. The invention also relates to a reagent kit for carrying out this method.
    Type: Application
    Filed: March 18, 2013
    Publication date: February 12, 2015
    Inventor: Delphine Jaga
  • Publication number: 20150038364
    Abstract: A microarray substrate including a piece of fluoropolymer whose surface is modified with polydopamine, in which the polydopamine forms an array of microspots on the surface of the fluoropolymer piece, and allows immobilization of molecules or cells. A microarray including the substrate, a microfluidic system designed for dispensing reagents onto selected locations on the surface of substrates, and a method for preparing the substrate and the microarray, in which a dopamine solution is dispensed onto the fluoropolymer piece using the microfluidic system, and forms an array of polydopamine microspots serving as the reaction sites for microarray analysis.
    Type: Application
    Filed: July 29, 2014
    Publication date: February 5, 2015
    Inventors: Bo Zheng, Hui Feng
  • Publication number: 20150037317
    Abstract: The present disclosure relates to a method for selecting a pool of molecules comprising detecting if the pool of molecules has binding specificity to an agent. A method for selecting a pool of biological markers in or on a cell, a composition comprising a pool of molecules, a method for delivering a therapeutic agent, and a method for diagnosing a condition in a subject are also provided.
    Type: Application
    Filed: December 4, 2012
    Publication date: February 5, 2015
    Inventors: Hardy W. Chan, Chi-Ying Huang
  • Publication number: 20150038354
    Abstract: Herein is reported a method of selecting a cell expressing a bispecific antibody comprising the steps of (a) generating a population of eukaryotic cells by transduction with a population of lentiviral virus particles, whereby each cell of the population of cells displays a membrane-bound full length antibody which is encoded by the lentiviral nucleic acid, and which specifically binds to two or more antigens or two or more epitopes on the same antigen, and (b) selecting from the population of eukaryotic cells a cell depending on the properties of the displayed membrane-bound full length antibody, whereby each lentiviral virus particle of the population of lentiviral virus particles comprises a bicistronic expression cassette comprising the EV71-IRES for the expression of the membrane-bound antibody.
    Type: Application
    Filed: June 20, 2014
    Publication date: February 5, 2015
    Applicant: HOFFMANN-LA ROCHE INC.
    Inventors: PETER MICHAEL HUELSMANN, HENDRIK KNOETGEN
  • Publication number: 20150031756
    Abstract: The invention relates to compositions containing polynucleotide vectors capable of expressing a nucleic acid encoding a fusion polypeptide on the surface of a viral particle and/or a eukaryotic cell.
    Type: Application
    Filed: August 1, 2014
    Publication date: January 29, 2015
    Inventor: Wayne A. Marasco
  • Publication number: 20150031583
    Abstract: A phage display system is provided comprising a mutant phage-infected host cell adapted to express a peptide of interest fused to a phage capsid protein, wherein the mutant phage includes a nonsense mutation which prevents expression of the capsid protein as a functional protein, and wherein expression of the peptide of interest is controlled by an inducible repressor and by a suppressor that suppresses the nonsense mutation.
    Type: Application
    Filed: April 24, 2014
    Publication date: January 29, 2015
    Inventors: Roderick Slavcev, Jessica Nicastro