Library, Per Se (e.g., Array, Mixture, In Silico, Etc.) Patents (Class 506/13)
  • Patent number: 9995732
    Abstract: Lipid multilayer structures are formed by evaporating a solvent from each of a plurality of lipid solutions thereby form the lipid multilayer structures. Each lipid solution comprises the solvent and one or more lipids. Each lipid multilayer structure is a microstructure comprising one or more lipids.
    Type: Grant
    Filed: February 12, 2014
    Date of Patent: June 12, 2018
    Assignee: FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: Steven Lenhert, Nicholas Vafai
  • Patent number: 9376710
    Abstract: Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.
    Type: Grant
    Filed: January 8, 2015
    Date of Patent: June 28, 2016
    Assignee: ILLUMINA CAMBRIDGE LTD.
    Inventors: Mark Edward Brennan Smith, Andrea Sabot, Isabelle Marie Julia Rasolonjatovo, Jean-Ernest Sohna Sohna, Adrian Martin Horgan, Harold Philip Swerdlow
  • Patent number: 9074300
    Abstract: The present invention provides a method, comprising (a) providing a reactant ligand attached to a substrate; (b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide; and (c) analyzing said display polypeptide.
    Type: Grant
    Filed: February 4, 2011
    Date of Patent: July 7, 2015
    Assignee: The University of Chicago
    Inventors: Milan Mrksich, Christian Hodneland
  • Patent number: 9046514
    Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance.
    Type: Grant
    Filed: February 11, 2008
    Date of Patent: June 2, 2015
    Assignees: ADVANCED LIQUID LOGIC, INC., DUKE UNIVERSITY
    Inventors: Ramakrishna Sista, Vamsee K. Pamula, Michael G. Pollack, Vijay Srinivasan, Allen E. Eckhardt, Richard B. Fair
  • Publication number: 20150141293
    Abstract: The invention provides a method for increasing the order of an array of polymeric micelles or of nanoparticles on a substrate surface comprising a) providing an ordered array of micelles or nanoparticles coated with a polymer shell on a substrate surface and b) annealing the array of micelles or nanoparticles by ultrasonication in a liquid medium which is selected from the group comprising H2O, a polar organic solvent and a mixture of H2O and a polar organic solvent. In a related aspect, the invention provides the highly ordered arrays of micelles or nanoparticles obtainable by the methods of the invention.
    Type: Application
    Filed: May 15, 2013
    Publication date: May 21, 2015
    Inventors: Christian Williges, Christoph Morhard, Joachim P. Spatz, Robert B. Brunner
  • Patent number: 9034798
    Abstract: Capture compounds and collections thereof and methods using the compounds for the analysis of biomolecules are provided. In particular, collections, compounds and methods are provided for analyzing complex protein mixtures, such as the proteome. The compounds are multifunctional reagents that provide for the separation and isolation of complex protein mixtures. Automated systems for performing the methods also are provided.
    Type: Grant
    Filed: February 26, 2010
    Date of Patent: May 19, 2015
    Assignee: CAPROTEC BIOANALYTICS GMBH
    Inventors: Hubert Köster, Daniel Paul Little, Suhaib Mahmood Siddiqi, Matthew Peter Grealish, Subramanian Marappan, Chester Frederick Hassman, III, Ping Yip
  • Publication number: 20150133414
    Abstract: It was determined whether SNPs in SLC4A5 are associated with salt sensitivity of blood pressure (BP). Subjects consumed an isocaloric constant diet with a randomized order of 7 days low Na+ (10 mmol/d) and 7 days high Na+ (300 mmol/d) intake. Salt sensitivity was defined as a ?7 mm Hg increase in mean arterial pressure (MAP). 35 polymorphisms in 17 candidate genes were assayed. Association analyses with salt sensitivity revealed three variants that associated with salt sensitivity, two in SLC4A5 (rs7571842, rs10177833; P<0.001), and one in GRK4 (rs1801058; P=0.020). Paradoxical changes in blood pressure in response to changes in salt intake were also found associated with a SNP for DRD2 (rs6276). In conclusion, SLC4A5 variants are strongly associated with salt sensitivity of BP in Caucasian and a DRD2 SNP is a marker for paradoxical response to salt intake.
    Type: Application
    Filed: April 22, 2013
    Publication date: May 14, 2015
    Applicant: University of Virginia Patent Foundation
    Inventors: Robin A. Felder, Robert M. Carey, John E. Jones, Pedro A. Jose, Scott M. Williams
  • Publication number: 20150126404
    Abstract: A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Yanqing Tian, Liqiang Zhang, Fengyu Su, Deirdre Meldrum, Sean Buizer, Clifford L. Anderson, Laimonas Kelbauskas, Kristen Lee
  • Publication number: 20150119272
    Abstract: The invention provides a computer-implemented method and system for predicting quantitatively whether an adjuvant or neoadjuvant chemotherapeutic treatment will be or is being successful in treating an individual suffering from cancer.
    Type: Application
    Filed: April 30, 2013
    Publication date: April 30, 2015
    Applicant: ROYAL COLLEGE OF SURGEONS IN IRELAND
    Inventors: Jochen Prehn, Andreas Lindner, Heinrich Huber
  • Patent number: 9006150
    Abstract: Disclosed is a composition comprising the nucleic acid and a chemical compound, said composition forming a star structure defining 3 or more stems extending from a reaction center. The stems are formed by a nucleic acid duplex and the chemical compound has been formed in the reaction center as the reaction product of 3 or more chemical groups. The advantage of the composition is that a close proximity is provided between the chemical groups in the reaction center, thereby promoting a reaction. The invention also relates to a method for preparation of the composition. The advantage of the method is that it does not require the pre-synthesis of a large number of templates and that it is not dependent upon codon/anti-codon recognition for an encoded molecule to be formed.
    Type: Grant
    Filed: May 17, 2012
    Date of Patent: April 14, 2015
    Assignee: Vipergen ApS
    Inventors: Nils Jokob Vest Hansen, Peter Blakskjaer, Margit Haahr Hansen, Lars Kolster Petersen, Tara Renee Heitner
  • Publication number: 20150094232
    Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.
    Type: Application
    Filed: March 28, 2012
    Publication date: April 2, 2015
    Applicant: GnuBIO, Inc.
    Inventors: Adam Abate, John Lawrence Emhoff, Tony Hung, Adnan Moez Esmail, Sepehr Kiani, Pascaline Mary
  • Publication number: 20150087525
    Abstract: The present invention relates in general to the field of colorectal cancer detection, and more particularly, to plasma microRNAs for the detection of early colorectal cancer.
    Type: Application
    Filed: October 20, 2012
    Publication date: March 26, 2015
    Applicant: HOSPITAL CLINIC DE BARCELONA
    Inventors: I Cos Meritxell Gironella, Juan Jose Lozano Salvatella, i Garangou Antoni Castells, Maria Dolores Giraldez
  • Publication number: 20150057161
    Abstract: The invention is based on the finding of specific surface markers for M1-like (classically activated) and M2-like (alternatively activated) macrophages and provides for a method for the identification, characterization and isolation of M1-like and M2-like macrophages based on the abundance of said surface markers and for means for performing such method.
    Type: Application
    Filed: March 13, 2013
    Publication date: February 26, 2015
    Applicants: BECTON DICKINSON AND COMPANY, RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITÄT-BONN
    Inventors: Joachim Schultze, Michael Mallmann
  • Publication number: 20150051102
    Abstract: The present disclosure provides compositions and methods useful for molecular and cellular separation, detection and quantification. The compositions provided herein comprise a nanostructure having magnetic property operably linked to an analyte-binding member.
    Type: Application
    Filed: April 1, 2013
    Publication date: February 19, 2015
    Inventors: Aihua Fu, James Zhu
  • Patent number: 8951755
    Abstract: The use of VHHs in the preparation of products to provide stability of antibody specificity under destabilizing conditions whereby normally lower eukaryotes or traditional antibodies are killed or inactivated.
    Type: Grant
    Filed: March 12, 1999
    Date of Patent: February 10, 2015
    Assignee: BAC IP B.V.
    Inventors: Daniel Convents, Leo G. Frenken, Michael M. Harmsen, Richard H. van der Linden, Cornelis T. Verrips
  • Publication number: 20150038364
    Abstract: A microarray substrate including a piece of fluoropolymer whose surface is modified with polydopamine, in which the polydopamine forms an array of microspots on the surface of the fluoropolymer piece, and allows immobilization of molecules or cells. A microarray including the substrate, a microfluidic system designed for dispensing reagents onto selected locations on the surface of substrates, and a method for preparing the substrate and the microarray, in which a dopamine solution is dispensed onto the fluoropolymer piece using the microfluidic system, and forms an array of polydopamine microspots serving as the reaction sites for microarray analysis.
    Type: Application
    Filed: July 29, 2014
    Publication date: February 5, 2015
    Inventors: Bo Zheng, Hui Feng
  • Publication number: 20150018247
    Abstract: The present invention provides a method of manufacturing biomedical molecular detection platform, comprising providing a plurality of reagent droplets on a first surface of a substrate; forming a plurality of hydrophilic regions and a water-repellant region on a second surface of a test paper, and the plurality of hydrophilic regions separated individually by the water-repellant region; and contacting the first surface of the substrate with the second surface of the test paper for transferring each reagent droplet on the substrate to each hydrophilic region of the test paper. The present invention also provides a biomedical molecular detection platform manufactured therefrom. The method of present invention can rapidly manufacture large quantity of biomedical molecular detection platforms, and the biomedical molecular detection platform can be used to any test that use pigmentation to determine results.
    Type: Application
    Filed: January 29, 2014
    Publication date: January 15, 2015
    Applicant: National Tsing Hua University
    Inventors: Fan-Gang Tseng, Shueh-Yao Chu, Chun-Wei Lee, Yu-Lin Wang, Shang-Chi Lin
  • Patent number: 8932994
    Abstract: The invention relates to a method of preparing a library of template polynucleotides with uniform sequence representation and to use of a library of templates prepared using this method for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5? ends and at their 3? ends, which contains even representation of all the fragments present in a starting sample of nucleic acid before fragmentation. The invention is especially applicable to the preparation of short insert libraries, where the sample fragments are less than 150 base pairs in length.
    Type: Grant
    Filed: August 23, 2007
    Date of Patent: January 13, 2015
    Assignee: Illumina, Inc.
    Inventors: Niall Anthony Gormley, Melanie Anne Smith
  • Publication number: 20140378329
    Abstract: A library of fecal samples from animals populations for analysis of disease in the population and methods for collection and analysis of the samples. Methods for managing a population of animals using the library and methods.
    Type: Application
    Filed: June 23, 2014
    Publication date: December 25, 2014
    Inventor: Matthew Howard Myles
  • Patent number: 8916504
    Abstract: The present invention features improved methods of in vitro RNA display to allow reliable expression and selection of scFv antibody molecules from expression libraries. The improved methods, in part, involve the use of mildly reducing conditions, which favor of scFv intra-chain disulphide bond and thus correct folding of the scFv antibody molecules. Although particularly suited to expression and selection of scFv antibody molecules, the methods of the invention are also expedient for in vitro RNA display of all classes of protein.
    Type: Grant
    Filed: September 30, 2009
    Date of Patent: December 23, 2014
    Assignee: Abbvie Inc.
    Inventors: Chung-Ming Hsieh, Yuliya A. Kutskova, John E. Memmott
  • Publication number: 20140356411
    Abstract: The present invention includes nanotubes or rods, methods and arrays using plasmonic-magnetic bifunctional nanotubes or rods comprising: one or more silica nanotubes or rods; one or more nanomagnets embedded in a portion of the silica nanotubes or rods; and plasmonic metal nanoparticles uniformly coating in or on at least a portion of the surface of the nanomagnets and the silica nanotubes surface-coated.
    Type: Application
    Filed: June 4, 2014
    Publication date: December 4, 2014
    Inventors: Donglei Fan, Xiaobin Xu
  • Publication number: 20140349888
    Abstract: Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.
    Type: Application
    Filed: August 7, 2014
    Publication date: November 27, 2014
    Inventors: John J. Rajasekaran, Vasanth Jayaraman, Tianhao Wang, Kang Bei, Hari Krishnan Krishnamurthy
  • Patent number: 8869591
    Abstract: A method for sensing analyte. The method includes the steps of sensing one or more parameters in reaction to the presence of one or more analytes and outputting a current therefrom in accordance with level of the sensed parameter by each of a plurality of sensors, each of the plurality of sensors being provided in one or more sensor array columns, selectively heating one or more of the sensor array columns by a heating element, and receiving an output current from one of the plurality of sensors from each of the plurality of sensor arrays by a Voltage Controlled Oscillator (VCO) arranged in a VCO array. The method further includes the steps of generating an output oscillation frequency by each VCO in accordance with the level of the received output current, and counting a number of oscillations over a predetermined time received from each of the plurality of VCOs in the VCO array by a plurality of counters arranged in a counter array.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: October 28, 2014
    Assignee: Sensorbit Systems, Inc.
    Inventors: Klaus Dimmler, Thomas Moss, III
  • Patent number: 8859469
    Abstract: A protein chip including at least a substrate having a plurality of steps which are regularly arranged on one surface thereof; a plurality of metallic microstructures arranged in the steps; and a lipid vesicle in which an outer surface thereof is modified by a functional group and a protein is present in a lipid bilayer thereof. The metallic microstructures and the lipid vesicle are bound via the functional group to provide the protein on the substrate.
    Type: Grant
    Filed: January 11, 2008
    Date of Patent: October 14, 2014
    Assignees: Nippon Telegraph and Telephone Corporation, Isis Innovation Limited
    Inventors: Chandra Sekar Ramanujan, Koji Sumitomo, Maurits R. R. de Planque, Hiroki Hibino, John F. Ryan, Keiichi Torimitsu
  • Patent number: 8853134
    Abstract: Some embodiments of the invention are directed to a microarray of binary nucleic acid probes for the detection of one or a plurality of nucleic acid analytes in a complex sample in a single high throughput assay with extraordinary specificity under physiologic conditions. Any binary nucleic acid probes that generate a detectable signal when bound to analyte are suitable for use in the microarrays, including binary deoxyribozyme or ribozyme probes; nonenzymatic binary probes for fluorescent detection, nonenzymatic binary dye-binding probes, and binary split enzyme peroxidase probes for visual detection of nucleic acids. The invention is also directed to new non nonenzymatic binary probes that bind to reporter oligonucleotides.
    Type: Grant
    Filed: January 28, 2010
    Date of Patent: October 7, 2014
    Assignee: The Trustees of Columbia University in the City of New York
    Inventor: Dmitry Kolpashchikov
  • Publication number: 20140287959
    Abstract: Polymer Pen Lithography is used to induce bioorthogonal reactions between treated surfaces and functionalized inks create a soft matter layer. Fluorescent and redox-active inks were used to demonstrate that the molecules were immobilized covalently and achieves precise control over ligand orientation and density within each feature. Finally, the utility was demonstrated by creating functional arrays of biologically active probes.
    Type: Application
    Filed: April 3, 2012
    Publication date: September 25, 2014
    Applicant: New York University
    Inventors: Adam B. Braunschweig, Jiajun He, Shudan Bian, Kevin B. Schesing
  • Publication number: 20140287958
    Abstract: The invention provides a liquid cell for an atomic force microscope. The liquid cell includes a liquid cell housing with an internal cavity to contain a fluid, a plurality of conductive feedthroughs traversing the liquid cell housing between the internal cavity and a dry side of the liquid cell, a cantilevered probe coupled to the liquid cell housing, and a piezoelectric drive element disposed on the cantilevered probe. The cantilevered probe is actuated when a drive voltage is applied to the piezoelectric drive element through at least one of the conductive feedthroughs. A method of imaging an object in a liquid medium and a method of sensing a target species with the liquid cell are also disclosed.
    Type: Application
    Filed: June 9, 2014
    Publication date: September 25, 2014
    Inventors: Jesse D. Adams, Benjamin S. Rogers, Todd A. Sulchek
  • Publication number: 20140274751
    Abstract: Provided herein is technology relating to identifying unknown compounds and particularly, but not exclusively, to methods and systems for identifying unknown compounds by gas chromatography-mass spectrometry by use of retention index as a second dimension for identification.
    Type: Application
    Filed: August 3, 2012
    Publication date: September 18, 2014
    Applicant: SMITHS DETECTION INC.
    Inventors: Charles Sadowski, Greger Andersson, Kevin Judge
  • Patent number: 8808984
    Abstract: The invention relates to a method for synthesizing templated molecules attached to the templated which directed the synthesis thereof. The method involves a template, a scaffold functional entity and a functional entity attached to a building block, which, in turn, is attached the template. The scaffold functional entity and the functional entity of the building block are both provided with complementary dimerization domains allowing the functional entities to come into close proximity when the complementary domains interact with to each other. The method may be used for generating libraries of templated molecules which may be selected for biological activity.
    Type: Grant
    Filed: July 24, 2008
    Date of Patent: August 19, 2014
    Assignee: Neuvolution A/S
    Inventors: Henrik Pedersen, Anette Holtmann, Thomas Franch, Alex Haahr Gouliaev, Jakob Felding
  • Publication number: 20140228249
    Abstract: An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.
    Type: Application
    Filed: April 1, 2014
    Publication date: August 14, 2014
    Applicant: Sentilus, Inc.
    Inventors: Ashutosh CHILKOTI, Angus HUCKNALL
  • Patent number: 8796184
    Abstract: An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.
    Type: Grant
    Filed: March 17, 2009
    Date of Patent: August 5, 2014
    Assignee: Sentilus, Inc.
    Inventors: Ashutosh Chilkoti, Angus Hucknall
  • Patent number: 8791053
    Abstract: The invention relates to a spatially encoded polymer matrix in the form of a bead or a granule for combinatorial solid phase synthesis, assaying, functional proteomics and diagnostic use. Compositions of such beads or granules are also provided. Each beaded polymer matrix of the composition comprises a plurality of spatially immobilized particles. The spatial immobilization of the particles confers on each beaded polymer matrix a “fingerprint” which enables identification of unique beads in a population of beads. The unique identification of individual beads makes it possible to perform combinatorial chemistry strategies while logging individual chemical transformation. Also provided are methods for detection of relative positions in space of particles, methods for generating matrices, methods for distance matrix determination, methods for identifying individual matrices and devices for recording and storing images of matrices.
    Type: Grant
    Filed: September 26, 2003
    Date of Patent: July 29, 2014
    Assignee: MPM-Holding APS
    Inventors: Soren Flygenring Christensen, Jens Hog Truelsen, Morten Meldal, Roice Michael, Ib Johannsen
  • Patent number: 8765642
    Abstract: Provided herein is a set of reagents comprising: a plurality of at least three probe libraries, wherein each library of the plurality comprises one or more probe sets that are each specific for a target; and at least one of the libraries comprises a probe set that is present in another of the libraries. The plurality of libraries can be hybridized to spatially separated targets, simultaneously or sequentially. The identity of a spatially separated target can be determined by identifying which combination of the multiple libraries hybridize thereto.
    Type: Grant
    Filed: April 17, 2012
    Date of Patent: July 1, 2014
    Assignee: Agilent Technolgies, Inc.
    Inventor: Bo U. Curry
  • Patent number: 8735167
    Abstract: Provided is a photoamplified fluorescence turn-off assay where a masked photosensitizer is mixed with a fluorescent molecule. This mixture is brightly fluorescent because the masked photosensitizer is not capable of quenching the fluorophore. When the photosensitizer is released and amplified, the photosensitizer quenches the emission of fluorophores very efficiently.
    Type: Grant
    Filed: August 20, 2008
    Date of Patent: May 27, 2014
    Assignee: Colorado Seminary, which owns and operates The University of Denver
    Inventors: Andrei G. Kutateladze, Alexei Kurchan, Rudresha Kottani, Janaki Majjigapu
  • Patent number: 8728988
    Abstract: A probe array base including probe-holding portions which are periodically arranged on a solid base member and which have grooves is prepared by anisotropically etching a single-crystalline silicon substrate. Probe solutions are supplied to the probe-holding portions by capillary action from a plurality of tank arrays having a certain cylinder period. This allows a probe array to be completed. The probe array is used as, for example, a DNA or antigen chip, has a high degree of integration, and is capable of holding a constant and sufficient number of probe molecules.
    Type: Grant
    Filed: June 4, 2012
    Date of Patent: May 20, 2014
    Assignee: Murata Manufacturing Co., Ltd.
    Inventor: Teruhisa Shibahara
  • Publication number: 20140113829
    Abstract: Systems and methods of selecting combinatorial coordinately dysregulated biomarker subnetworks are provided. In one embodiment, a method comprises comparing the normalized gene expression data to a predetermined threshold to provide binary gene expression data associated with phenotype samples and control samples, analyzing subnetwork states of the binary gene expression data associated with phenotype samples and control samples to identify gene expression patterns that occur in phenotype samples and do not occur in control samples and identifying a subnetwork that provides gene expression patterns indicative of a sample being a phenotype sample.
    Type: Application
    Filed: April 22, 2011
    Publication date: April 24, 2014
    Applicant: CASE WESTERN RESERVE UNIVERSITY
    Inventors: Mehmet Koyuturk, Mark Chance, Rod Nibbe, Salim Akhter Chowdhury
  • Publication number: 20140106980
    Abstract: A sensor having a substrate is provided in which structures are disposed on a surface of the substrate. The structures can be, e.g., nanostructures. Polarized light is directed toward the sensor, and birefringence of the structures with respect to the light is measured. Target particles that interact with the structures are detected based on changes in the measured birefringence.
    Type: Application
    Filed: October 15, 2013
    Publication date: April 17, 2014
    Inventors: Mathias M. Schubert, Tino Hofmann, Daniel Schmidt, Patrick H. Dussault, Andrea Holmes, Rebecca Y. Lai
  • Patent number: 8697604
    Abstract: Provided is a method for characterizing a molecule by mass spectrometry, which molecule comprises one or more free amino groups, which method comprises: (a) reacting one or more free amino groups in the molecule with a mass tag reagent comprising a reactive functionality capable of reacting with an amino group, and a tertiary amino group linked to the reactive functionality; and (b) characterizing the molecule by mass spectrometry.
    Type: Grant
    Filed: March 18, 2004
    Date of Patent: April 15, 2014
    Assignee: Electrophoretics Limited
    Inventors: Christian Hamon, Karsten Kuhn, Andrew Thompson, Dieter Reuschling, Juergen Schaefer
  • Publication number: 20140100123
    Abstract: A plurality of isolated microvessels including a plurality of encoded microvessels each having a microbody and a reservoir core. The microbody is configured to separate a biological or chemical substance in the reservoir core from an ambient environment surrounding the microbody. The microbody includes a transparent material that at least partially surrounds the reservoir core and facilitates detection of an optical characteristic of the substance within the reservoir core. The microbody of each microvessel includes an identifiable code that distinguishes individual microvessels of the plurality of encoded microvessels from each other. The plurality of isolated microvessels also includes a plurality of compartments each configured to separate individual microvessels of the plurality of encoded microvessels from each other.
    Type: Application
    Filed: August 16, 2013
    Publication date: April 10, 2014
    Applicant: Illumina, Inc.
    Inventors: John A. Moon, M. Shane Bowen, Ryan C. Smith, Michel Perbost, Michal Lebl, Steven H. Modiano
  • Patent number: 8685892
    Abstract: The present invention relates to methods, compositions and systems for detecting perchlorate in a sample. Compositions useful for detecting perchlorate in a sample include those comprising a perchlorate reductase, a reductant and an electron shuttle. In an exemplary embodiment, the composition comprises perchlorate reductase from Dechloromonas agitata, reduced nicotinamide adenine dinucleotide and N-methylphenazinium methylsulfate. The present invention also provides for methods of using the compositions disclosed herein, as well as systems thereof. In some exemplary embodiments, the methods comprise a concentration step, in which, for example, the sample is contacted with a cationic solid phase extraction column. Employing this step provides certain advantages such as a lowered detection limit and the removal of contaminants.
    Type: Grant
    Filed: September 1, 2009
    Date of Patent: April 1, 2014
    Assignee: The Regents of the University of California
    Inventors: John D. Coates, Mark L. Heinnickel
  • Patent number: 8680020
    Abstract: Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis.
    Type: Grant
    Filed: July 15, 2009
    Date of Patent: March 25, 2014
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Chung-Yi Wu, Susan Y. Tseng
  • Patent number: 8673622
    Abstract: Disclosed herein are a cell chip and a method for manufacturing the same. The cell chip may include: a substrate; and a first contact member disposed on the substrate, wherein a top end of the first contact member is provided with a first inclined contact surface inclined with respect to the substrate.
    Type: Grant
    Filed: May 29, 2012
    Date of Patent: March 18, 2014
    Assignee: Samsung Electro-Mechanics Co., Ltd.
    Inventors: Dong Woo Lee, Sang Hyun Yi, Bo Sung Ku
  • Patent number: 8652833
    Abstract: To provide a cell culture chamber capable of reproducing a cell function in vivo, and a method of producing the same. The cell culture chamber according to the present invention has a concave-convex pattern formed on the surface on which cells are cultured. A concave portion of the concave-convex pattern includes cell culture portions and micro flow channels communicating with the cell culture portions. The bottom surface of each of the cell culture portions has a width 1.0 to 20 times an equivalent diameter of each of the cultured cells. A cell adhesion-inducing substance is formed only on the bottom surface and the side walls of the concave portion.
    Type: Grant
    Filed: April 25, 2007
    Date of Patent: February 18, 2014
    Assignee: Kuraray Co., Ltd.
    Inventors: Yuji Morimoto, Taiji Nishi, Taisuke Kamada, Go Tazaki, Motohiro Fukuda
  • Patent number: 8652417
    Abstract: A set of crystallization solutions includes a plurality of crystallization solutions of Crystallization Solution Set I, a plurality of crystallization solutions of Crystallization Solution Set II or a plurality of crystallization solutions of Crystallization Solution Set III.
    Type: Grant
    Filed: January 20, 2011
    Date of Patent: February 18, 2014
    Assignee: Microlytic APS
    Inventor: Morten Sommer
  • Patent number: 8647591
    Abstract: A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber.
    Type: Grant
    Filed: January 17, 2011
    Date of Patent: February 11, 2014
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Won-seok Chung, Myo-yong Lee, Kak Namkoong, Woochang Lee
  • Patent number: 8642512
    Abstract: Provided are methods of diagnosing an early immune activation by detecting T-cell immune response cDNA 7 (TIRC7); in particular, TIRC7 as an early biomarker for the detection of transplant rejection in a non-invasive diagnostic methods is described that replaces biopsy intervention with a simple diagnostic method for monitoring after transplantation and furthermore, kits for uses in such methods of diagnosis are provided.
    Type: Grant
    Filed: May 17, 2006
    Date of Patent: February 4, 2014
    Assignee: Cellact Pharma GmbH
    Inventor: Nalân Utku
  • Patent number: 8642516
    Abstract: Patterned SAM arrays and methods of preparing patterned SAM arrays are disclosed. Advantageously, the methods used to prepare the patterned SAM arrays allow for controlling SAM spot-to-spot conditions such as ligand identity and ligand density, which allows for preparing a wide range of SAM spots in a single array format. Additionally, the patterned SAM arrays of the present disclosure support the culture of a range of cell types. The patterned SAM arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.
    Type: Grant
    Filed: May 7, 2012
    Date of Patent: February 4, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Justin T. Koepsel, William L. Murphy
  • Patent number: 8637436
    Abstract: A biosensor array, system and method for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Grant
    Filed: August 24, 2007
    Date of Patent: January 28, 2014
    Assignee: California Institute of Technology
    Inventor: Arjang Hassibi
  • Publication number: 20140007286
    Abstract: The present invention relates to a method for genetically modifying the genome of a hybrid plant by replacing one or more of its chromosomes or chromosome fragments with the corresponding chromosome or chromosome fragment of one or more donor parents. The invention uses the reverse breeding technique to construct a novel type of substitution and introgression libraries that can be used for accelerated breeding and hybrid correction. These libraries and their use are also part of this invention.
    Type: Application
    Filed: March 11, 2013
    Publication date: January 2, 2014
    Applicant: Rijk Zwaan Zaadteelt en Zaadhandel B.V.
    Inventor: Robert Hélène Ghislain
  • Publication number: 20130345088
    Abstract: This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41?) into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41?) and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).
    Type: Application
    Filed: March 7, 2012
    Publication date: December 26, 2013
    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Hiroyuki Noji, Ryota Iino, Suguru Araki