Library Contained In Or Displayed By A Micro-organism (e.g., Bacteria, Animal Cell, Etc.) Or Library Contained In Or Displayed By A Vector (e.g., Plasmid, Etc.) Or Library Containing Only Micro-organisms Or Vectors Patents (Class 506/14)
-
Publication number: 20120028841Abstract: Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies.Type: ApplicationFiled: June 15, 2011Publication date: February 2, 2012Inventor: Robert Charles Ladner
-
Publication number: 20120028840Abstract: Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies.Type: ApplicationFiled: June 15, 2011Publication date: February 2, 2012Applicant: Dyax Corp.Inventor: Robert Charles Ladner
-
Publication number: 20120021952Abstract: Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies.Type: ApplicationFiled: September 30, 2011Publication date: January 26, 2012Inventor: Robert Charles Ladner
-
Publication number: 20120015841Abstract: The invention relates to novel cells and cell lines, and methods for making and using them.Type: ApplicationFiled: February 1, 2010Publication date: January 19, 2012Applicant: CHROMOCELL CORPORATIONInventors: Kambiz Shekdar, Dennis Sawchuk, Purvi Manoj Shah
-
Patent number: 8097565Abstract: The present invention relates to a process comprising forming a monomer mixture comprising at least one monofunctional silicone containing component which comprises at least one difunctional byproduct and adding to said monomer mixture a normalizing amount of said at least one difunctional byproduct and curing said monomer mixture to form a biomedical device.Type: GrantFiled: June 4, 2004Date of Patent: January 17, 2012Assignee: Johnson & Johnson Vision Care, Inc.Inventors: Gregory A. Hill, John C. Heaton, Michael R. Clark, Shivkumar Mahadevan, Carrie L Caison, Frank F. Molock
-
Publication number: 20120010101Abstract: The present disclosure relates to novel triple tag sequences that may comprise a 6× histidine tag, a c-myc tag and a V5 tag. The present disclosure also provides polynucleotides, proteins, vectors and host cells that comprise the triple tag sequence of the present disclosure, including libraries of such polynucleotides, proteins, vectors and host cells. The novel triple tag sequences of the present disclosure may be used in phage display vectors and phage libraries and in methods for detection, screening, capture, purification, quantitation, and/or recovery of proteins of interest to which they are linked. Proteins of interest include antibodies such as single chain antibodies, single chain antibodies, and Fab fragments of antibodies or peptides such as non-antibody peptides.Type: ApplicationFiled: September 26, 2011Publication date: January 12, 2012Applicant: XOMA Technology, Ltd.Inventors: Isaac Rondon, Marina Roell, Daniel Bedinger
-
Publication number: 20110318269Abstract: The present invention relates to a method for screening phage display libraries against each other. In particular, the invention relates to a method for screening at least two phage display libraries against each other to identify and/or select one or more interacting binding partners or binding molecules making up such interacting binding partners. Kits providing two bispecific phage display libraries are also provided.Type: ApplicationFiled: February 26, 2010Publication date: December 29, 2011Applicant: NEXTERA ASInventors: Herald Reiersen, Geir Age Loset, Urs Beat Hagemann
-
Patent number: 8084574Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: December 27, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Patent number: 8084573Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: December 27, 2011Assignee: E.I. Du Pont de Nemours and CompanyInventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Publication number: 20110312543Abstract: The present invention relates to expression vector comprising (a) a promoter region comprising a non-inducible constitutively active ribosomal protein gene promoter, (b) an operably linked reporter or gene sequence, and (c) a 3? untranslated region (3? UTR), which are suitable means for an selective assessment of post-transcriptional regulation, post-transcriptional control elements and factors as well as for identifying compounds that effect post-transcription. The present invention furthermore relates to arrays, expression vector libraries and cell lines containing the expression vector(s). The present invention furthermore relates to a method and kit for identifying compounds that affect post-transcriptional regulation of reporter(s) or gene(s), that utilize the expression vector(s).Type: ApplicationFiled: November 17, 2008Publication date: December 22, 2011Inventor: Khalid S. Abu Khabar
-
Publication number: 20110301064Abstract: The present invention provides an alternative scaffold for peptides displayed on filamentous phages through novel fusion proteins primarily originating from pIX. Libraries of filamentous phages can be created from fusion proteins, and a phage display system comprising a phagemid and a helper phage is a part of the invention. An aspect of the invention is a kit containing a phage display system comprising a phagemid that contains a nucleic acid encoding the fusion protein of the invention and a helper phage.Type: ApplicationFiled: February 24, 2010Publication date: December 8, 2011Applicant: Nextera ASInventor: Geir Åge Løset
-
Publication number: 20110302666Abstract: A spermatogonial stem cell line that is derived from testes rats characterized by a desirable genetic background can serve as a source for cells to transplant into male-sterile recipient animals that are immuno-compatible with the spermatogonial line. Rat cells thus transplanted readily develop into fertilization-competent, haploid male gametes, with little or no endogenous sperm competition generated by the testes of the male-sterile recipient. This approach, constituting the first vector system for the use of rat spermatogonial lines from in vitro culture in generating mutant rats on a desired genetic background, effects maximal germline transmission of donor haplotypes from the transplanted spermatogonial cells.Type: ApplicationFiled: December 1, 2009Publication date: December 8, 2011Inventor: Franklin Kent Hamra
-
Patent number: 8067534Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: November 29, 2011Inventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Publication number: 20110287971Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).Type: ApplicationFiled: June 28, 2011Publication date: November 24, 2011Applicant: Dyax Corp.Inventors: Simon E. Hufton, Hendricus R.J.M. Hoogenboom
-
Publication number: 20110287979Abstract: The invention relates to novel polypeptides and cells comprising the polypeptides. The polypeptides and cells are used in methods to identify and/or isolate cells producing a protein with specific biological functions. In particular, the methods may be used for identifying, selecting, and isolating cells producing antigen-specific monoclonal antibodies.Type: ApplicationFiled: February 11, 2011Publication date: November 24, 2011Applicant: OncoMed Pharmaceuticals, Inc.Inventors: Austin L. Gurney, Alexandra L. L. Lazetic, Christopher J. Bond
-
Publication number: 20110287978Abstract: A cell chip is provided which includes a first substrate having a micro channel extending from an upper surface thereof to a lower surface or a side surface thereof, and a first bio matrix arranged on the upper surface of the first substrate to cover the micro channel while containing cells. The cell chip supplies fluid to cells contained in the bio matrix by means of perfusion and diffusion, thereby providing an environment similar to a biological environment.Type: ApplicationFiled: September 14, 2010Publication date: November 24, 2011Applicant: SAMSUNG ELECTRO-MECHANICS CO., LTD.Inventors: Dong Woo LEE, Se Hoon JEONG, Sang Jin KIM, Jeong Suong YANG, Bo Sung KU
-
Patent number: 8063178Abstract: Trp cage binding domains polypeptides are disclosed. The Trp cage binding domains have the generic formulae of SEQ ID NO: 2, 7, 10 or 11. They can be efficiently produced and screened using phage display technology.Type: GrantFiled: December 12, 2007Date of Patent: November 22, 2011Assignee: Marina Biotech, Inc.Inventors: Steven C. Quay, Douglas L. Badders, Richard E. Herman, Michael E. Houston, Jr., Paul Hickok Johnson
-
Patent number: 8063179Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: November 22, 2011Assignee: E I du Pont de Nemours and CompanyInventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Publication number: 20110281764Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: ApplicationFiled: August 16, 2010Publication date: November 17, 2011Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: J. CHRISTOPHER LOVE, HIDDE L. PLOEGH, JEHNNA RONAN
-
Patent number: 8058392Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: November 15, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Publication number: 20110274744Abstract: The invention relates to processes for coating a surface with a crosslinked polyelectrolytes multilayer film incorporating a protein, preferably a growth factor type protein. The invention also relates to crosslinked polyelectrolytes multilayer films obtained by this process, and a coated surface obtained therefrom.Type: ApplicationFiled: January 15, 2010Publication date: November 10, 2011Applicants: INSTITUT POLYTECHNIQUE DE GRENOBLE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE MONTPELLIER 2 SCIENCES ET TECHNIQUESInventors: Catherine Picart, Thomas Crouzier
-
Publication number: 20110275535Abstract: The present invention relates to the field of protein display libraries and library screening. In preferred embodiments, the present invention provides a three component system for display comprising a cell surface molecule, an adapter molecule and a display molecule.Type: ApplicationFiled: December 14, 2009Publication date: November 10, 2011Applicant: NOVARTIS AGInventor: Andreas Loew
-
Publication number: 20110274623Abstract: A protein scaffold based on a consensus sequence of fibronectin type III (FN3) proteins, such as the tenth FN3 repeat from human fibronectin (human Tenascin), including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof, exhibit enhanced thermal and chemical stability while presenting six modifiable loop domains which can be engineered to form a binding partner capable of binding to a target for applications in diagnostic and/or therapeutic compositions, methods and devices.Type: ApplicationFiled: April 29, 2011Publication date: November 10, 2011Inventor: Steven Jacobs
-
Patent number: 8053555Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: November 8, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Publication number: 20110269645Abstract: Provided is a cell chip, a method of manufacturing the same, and a device for manufacturing the same. The cell chip includes a substrate on which a plurality of cell fixing materials having a predetermined area are arranged, a plurality of single cells fixed by the plurality of cell fixing materials, respectively, and a plurality of cell scattering materials formed of a porous material and provided in the form of droplets combined with the plurality of cell fixing materials and surrounding the single cells, respectively. The device for manufacturing the cell chip includes a first inkjet head discharging a cell fixing material having a predetermined area onto a substrate, and a second inkjet head including an internal channel having one or more curved portions, and discharging a plurality of cells, contained in a cell scattering material passing through the internal channel, individually to the cell fixing material.Type: ApplicationFiled: September 1, 2010Publication date: November 3, 2011Applicant: SAMSUNG ELECTRO-MECHANICS CO., LTD.Inventors: Se Hoon Jeong, Sang Jin Kim, Bo Sung Ku
-
Patent number: 8044176Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: October 25, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Publication number: 20110257037Abstract: Disclosed is a microfluidic device including a microfluidic structure formed in a platform in which various examinations, such as an immune serum examination, can be automatically performed using the biomolecule microarray chip. The biomolecule microarray chip-type microfluidic device using a biomolecule microarray chip comprises: a platform which is rotatable; a microfluidic structure disposed in the platform, comprising: a plurality of chambers; a plurality of channels connecting the chambers each other; and a plurality of valves controlling flow of fluids through the channels, wherein the microfluidic structure controls flow of a fluid sample using rotation of the platform and the valves; and a biomolecule microarray chip mounted in the platform such that biomolecule capture probes bound to the biomolecule microarray chip contact the fluid sample in the microfluidic structure.Type: ApplicationFiled: June 24, 2011Publication date: October 20, 2011Applicant: SAMSUNG ELECTRONICS CO ., LTD.Inventors: Beom-seok LEE, Jeong-gun Lee, Jong-myeon Park, Yoon-kyoung Cho
-
Patent number: 8039583Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: October 18, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Patent number: 8039584Abstract: Peptides have been generated that have binding affinity to carbon nanostructures and particularly carbon nanotubes. Peptides of or the invention are generally about twelve amino acids in length. Methods for generating carbon nanotube binding peptides are also disclosed.Type: GrantFiled: October 1, 2010Date of Patent: October 18, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Anand Jagota, Steven Raymond Lustig, Siqun Wang, Hong Wang
-
Publication number: 20110250203Abstract: The present invention relates to the field of compositions comprising anti-CD5 antibodies. In particular, the present invention concerns an antibody composition comprising at least two anti-CD5 antibodies capable of binding distinct CD5 epitopes. The invention further concerns bi-specific molecules having the binding specificities of said antibody compositions. The invention also relates topharmaceutical compositions, use of antibody compositions and methods for manufacturing antibody compositions. The invention further relates to cell banks and a method for killing cells.Type: ApplicationFiled: August 28, 2009Publication date: October 13, 2011Applicant: Symphogen A/SInventors: Josephine L. K. Klitgaard, Charles Pyke, Mikke Wandahl Pedersen, Klaus Koefoed
-
Publication number: 20110251106Abstract: The present invention provides an alternative scaffold for peptides displayed on filamentous phages through novel fusion proteins primarily originating from pVII. Libraries of filamentous phages can be created from fusion proteins, and a phage display system comprising a phagemid and a helper phage is a part of the invention. An aspect of the invention is a kit containing a phage display system comprising a phagemid and a helper phage that contains a nucleic acid encoding the fusion protein of the invention.Type: ApplicationFiled: August 20, 2008Publication date: October 13, 2011Applicant: NEXTERA ASInventor: Geir Åge LØset
-
Publication number: 20110251264Abstract: Methods and compositions for altering prokaryotic cellular viability phenotypes, including antibiotic susceptibility.Type: ApplicationFiled: October 2, 2009Publication date: October 13, 2011Inventors: Michael McArthur, Jane Marion Moore
-
Publication number: 20110245108Abstract: Universal antibody libraries are described which are synthetic and derived from expressed human antibody sequences selected accordingly to certain criteria, for example, that the sequences are derived from naturally-occurring antibodies expressed in response to a certain antigen class (e.g., small molecule, polysaccharide, peptide, or protein) and having CDR regions engineered for optimal diversity. Methods for making and screening such libraries for isolating therapeutics suitable for treating disease are also disclosed.Type: ApplicationFiled: July 6, 2005Publication date: October 6, 2011Inventors: Roberto Crea, Arvind Rajpal, Guido Cappuccilli, Randy Shen, Toshihiko Takeuchi
-
Publication number: 20110237461Abstract: The present disclosure provides compositions and methods for using one or more polypeptide probes to profile an immune response. The polypeptide probe can be used to detect one or more antibodies from a sample. Furthermore, the present disclosure provides methods and compositions for characterizing a cancer based on the detection of one or more antibodies, such as autoantibodies.Type: ApplicationFiled: March 17, 2011Publication date: September 29, 2011Applicants: THE REGENTS OF THE UNIVERSITY OF MICHIGAN, ARMUNE BIOSCIENCES, INC.Inventors: Arul M. Chinnaiyan, Xiaoju Wang, Alex Tsodikov, Jeanne Ohrnberger
-
Publication number: 20110237457Abstract: The present disclosure provides compositions and methods for using phage epitopes to profile the immune response. The phage epitopes can be used to detect one or more antibodies from a sample. Furthermore, the present disclosure provides methods and compositions for detecting a cancer based on the detection of one or more antibodies. In one embodiment, the antibody is an autoantibody.Type: ApplicationFiled: March 25, 2011Publication date: September 29, 2011Applicant: Armune Biosciences, Inc.Inventors: Jeanne OHRNBERGER, Stephen A. Spindler
-
Publication number: 20110224097Abstract: Viable Gram-negative bacteria or components thereof comprising outer membranes that substantially lack a ligand, such as Lipid A or 6-acyl lipidpolysaccharide, that acts as an agonist of TLR4/MD2. The bacteria may comprise reduced activity of arabinose-5-phosphate isomerases and one or more suppressor mutations, for example in a transporter thereby increasing the transporters capacity to transport Lipid IVA or in membrane protein YhjD. One or more genes (e.g., IpxL, IpxM, pagP, IpxP, and/or eptA) may be substantially deleted and/or one or more enzymes (e.g., LpxL, LpxM, PagP, LpxP, and/or EptA) may be substantially inactive. The bacteria may be competent to take up extracellular DNA, may be donor bacteria, or may be members of a library. The present invention also features methods of creating and utilizing such bacteria.Type: ApplicationFiled: March 11, 2011Publication date: September 15, 2011Applicant: RESEARCH CORPORATION TECHNOLOGIESInventors: David Bramhill, Uwe Mamat
-
Publication number: 20110190162Abstract: The invention is directed to a three-dimensional cell culture array comprising spatially-separated matrices attached to a solid support, wherein a plurality of said matrices encapsulate cells transfected with nucleic acids, method for the preparation of the array and methods reducing the expression of a target gene.Type: ApplicationFiled: November 12, 2010Publication date: August 4, 2011Inventors: Moo-Yeal Lee, Seok Joon Kwon, Jonathan S. Dordick, Douglas S. Clark, Jessica R. McKinley
-
Publication number: 20110190164Abstract: The field of the invention refers to chimeric Virus Like Particles (VLP) derived from Birnavirus chimeric VP2 protein. In particular, the invention refers to chimeric VP2 fusion proteins which incorporate insertions and/or substitutions with one or more amino acids or particular peptide of interest while maintaining the capacity to assemble in the form of VLP. The invention identifies particular insertion and/or substitutions sites within VP2 P loop regions and outside said P loop regions. The invention also incorporates methods for the identification of preferred insertion and substitution sites within VP2 for the incorporation of particular amino acids and peptides of interest. The resulting chimeric VLP are of interest in the design of therapeutic and prophylactic vaccines as well as in the design of drug delivery systems, carriers for DNA and RNA in gene therapy, as targeted agents, in the development of antitoxins, and as diagnostic reagents.Type: ApplicationFiled: May 9, 2008Publication date: August 4, 2011Inventors: Thomas Zurcher, Juan Jose Bernal, Cayetano von Kobbe, Ignacio JimenezTorres, Ana Diaz Blázquez, Diana Martin Lorenzo, Gloria Calderita Lucas, Arcadio Garcia de Castro
-
Publication number: 20110183868Abstract: The present invention relates to solution microarrays. In particular, the present invention relates to an aqueous 2-phase system for solution microarrays and uses thereof. Additional embodiments are described herein.Type: ApplicationFiled: August 4, 2009Publication date: July 28, 2011Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Shuichi Takayama, Hossein Tavana, Andreja Jovic, Bobak Mosadegh
-
Publication number: 20110177973Abstract: The present invention is concerned with the provision of screening assays.Type: ApplicationFiled: September 24, 2009Publication date: July 21, 2011Applicant: Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.Inventor: Moritz Rossner
-
Publication number: 20110177974Abstract: The present invention identities biomarkers that are diagnostic of nerve cell injury and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury.Type: ApplicationFiled: November 19, 2010Publication date: July 21, 2011Applicants: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC., BANYAN BIOMARKERS, INC.Inventors: KA-WANG (KEVIN) WANG, Ming-Cheng Liu, Monika Oli
-
Publication number: 20110171663Abstract: There is provided a cell culture microtrench being defined on or in a surface of a substrate, wherein the ratio of the width of the microtrench to the maximum length of the short axis of a cell type of interest is about 6 or preferably less, the length of the short axis of the cell type being measured when a cell is in detached or suspended form. There is also provided an array comprising such a microtrench and uses of such microtrenches, including cell-based assays.Type: ApplicationFiled: January 29, 2009Publication date: July 14, 2011Applicant: University College Cardiff Consultants LtdInventors: Paul J. Smith, Rachel J. Errington, Huw Summers, Sally Chappell
-
Publication number: 20110172120Abstract: The present invention provides compositions and methods of modulating or regulating eukaryotic gene expression through the controlled or regulated expression of polynucleotide constructs that encode siRNA or other desired exogenous nucleic acids or proteins. Such constructs, and additional elements of the system may be transfected into the cells of interest and the expression of the siRNA, and hence the expression of the target gene of the siRNA, may be controlled through the administration of a compound to the cell, such as a small molecule or drug. Lentivirus vectors are employed in some embodiments of the invention including the generation of conditional knockdown animals.Type: ApplicationFiled: July 6, 2007Publication date: July 14, 2011Inventors: Didier Trono, Maciej Wiznerowicz
-
Publication number: 20110172121Abstract: This invention provides methods for supplying a therapy for individuals exposed to radiation following a nuclear event, through the prospective establishment of an undesignated allogeneic stem cell bank with prospective HLA typing of healthy potential recipients.Type: ApplicationFiled: October 20, 2010Publication date: July 14, 2011Applicant: StemCyte, Inc.Inventors: Robert Chow, Lawrence D. Petz, Rubio R. Punzalan, Denis O. Rodgerson
-
Publication number: 20110152125Abstract: A substrate comprising a microporous microstructure, an interlayer over at least a portion of the microstructure and a functional layer attached to the interlayer, the functional layer having functional sites with a density of at least 50 nanomoles/cm2.Type: ApplicationFiled: March 1, 2011Publication date: June 23, 2011Inventors: Anit Dutta, Heidi Flannery, William P. Mortimer, JR.
-
Publication number: 20110152122Abstract: The invention includes compositions comprising a S. cerevisiae yeast library, and methods of identifying an epigenetic marker for the diagnosis of infertility or a disorder associated with gametogenesis in an individual.Type: ApplicationFiled: November 17, 2010Publication date: June 23, 2011Inventors: Shelley L. Berger, Jerome Govin
-
Publication number: 20110150845Abstract: The invention provides compositions and methods for modulating immune responses using mesenchymal stem cells. The invention further provides methods for inducing tolerance to self antigens using mesenchymal stem cells.Type: ApplicationFiled: May 1, 2009Publication date: June 23, 2011Applicants: MASSACHUSETTS INSTITUTE OF TECHNOLOGY, THE GENERAL HOSPITAL CORPORATION D/B/A MASSACHUSETTS GENERAL HOSPITAL, DANA-FARBER CANCER INSTITUTE, INC.Inventors: Biju Parekkadan, Martin Leon Yarmush, Shannon J. Turley
-
Publication number: 20110136688Abstract: Modified forms of naturally occurring bacteriocins, such as the R-type pyocins of Pseudomonas aeruginosa, are disclosed as are methods for producing them in GRAS organisms. The bacteriocins are modified at the ends of their tail fibers in a region responsible for binding specificity and affinity to their cognate binding partners, or receptors, such as those on the surface of bacteria. Methods for the use of the modified bacteriocins, such as to bind receptors, including virulence or fitness factors, on the surfaces of bacteria, are also described.Type: ApplicationFiled: November 8, 2010Publication date: June 9, 2011Applicant: AvidBiotics CorporationInventors: Dean SCHOLL, Steven Williams
-
Publication number: 20110136144Abstract: Methods of diagnosing Alzheimer's disease are provided. At least five methods of diagnostic measurements are presented: Method 1: Integrated score; Method 2: Average aggregate area per number of aggregates; Method 3: Cell migration analysis; Method 4; Fractal analysis; Method 5: Lacunarity Analysis. In certain embodiments, a sample of a subject's skin provides a network of fibroblasts that is imaged and a fractal dimension of the image is calculated. The fractal dimension can be compared to an aged-matched control (non-Alzheimer's) database to determine if the subject has Alzheimer's disease. The network of fibroblasts may be cultured in a matrix, for example in a protein mixture.Type: ApplicationFiled: October 2, 2010Publication date: June 9, 2011Inventors: Florin Valentin Chirila, Tapan Kumar Khan, Daniel L. Alkon
-
METHOD FOR CREATING A VIRAL GENOMIC LIBRARY, A VIRAL GENOMIC LIBRARY AND A KIT FOR CREATING THE SAME
Publication number: 20110130304Abstract: A method for creating an alphavirus-based genomic library, comprising a) ligation of foreign sequence (s) from an expression library or a random library into plasmids containing cloned alphaviral cDNA, b) multiplication of the obtained plasmid constructs in bacterial cells, c) direct transfection of the obtained plasmid constructs into mammalian or arthropod cells, characterized in that the sequence of an intron or sequences of introns are inserted into the respective genome of an alphavirus or into the cDNA of an expression vector based on an alphavirus, —the sequence of a viral subgenomic promoter, which is larger than minimal functional promoter is inserted immediately to the 3? end of the sequences coding the structural proteins of the named alphavirus, —and ribozyme sequence is inserted for creating correct 3? ends of the alphavirus.Type: ApplicationFiled: August 29, 2008Publication date: June 2, 2011Applicant: TARTU ULIKOOL (UNIVERSITY OF TARTU)Inventors: Kai Rauasalu, Anna Iofik, Valeria Lulla, Liis Karo-Astover, Kristi Tamm, Liane Ulper, Inga Sarand, Andres Merits