Solid-phase Synthesis (i.e., Wherein One Or More Library Building Blocks Are Bound To A Solid Support During Library Creation) Or Particular Method Of Cleavage From The Solid Support Used Patents (Class 506/30)
  • Patent number: 9725709
    Abstract: Embodiments of the present disclosure provide a multistage procedure for treatment of biological samples (e.g., living cells with membranes, and the like) with a substance (e.g., a drug, DNA, RNA, plasmids, and other biomolecules or materials) to achieve more efficacious intracellular delivery and transfection.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: August 8, 2017
    Assignee: OPENCELL TECHNOLOGIES, INC.
    Inventors: John Mark Meacham, Kiranmai Durvasula, Andrei G. Fedorov, Fahrettin Levent Degertekin, Akash Mehta
  • Patent number: 9217132
    Abstract: Provided herein are apparatuses and methods for fragmenting nucleic acids or disrupting cells. For example, some embodiments provide a disposable microfluidic device designed to position a sample to be in direct contact with a high frequency vibrating element that emits an ultrasonic frequency into the sample such that the vibrational energy transduced into the sample results in fragmenting nucleic acids or disrupting cells.
    Type: Grant
    Filed: January 20, 2012
    Date of Patent: December 22, 2015
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Mark W. Eshoo, Jose R. Gutierrez, Jared J. Drader, John Picuri, Karl M. Cabrera, Stanley Motley, Thomas N. Chiesl
  • Patent number: 9194000
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: November 24, 2015
    Assignee: Life Technologies Corporation
    Inventors: Jonathan M. Rothberg, Wolfgang Hinz, Kim L. Johnson, James Bustillo, John Leamon, Jonathan Schultz
  • Patent number: 9095852
    Abstract: A multilayer well device includes a first substrate comprising an array of wells having a first pattern disposed therein and a second substrate comprising an array of wells having a second pattern, complementary to the first pattern disposed therein, wherein the second substrate is secured adjacent to a face of the first substrate. A common channel is interposed between the array of wells of the respective first and second substrates and is coupled to an inlet and an outlet.
    Type: Grant
    Filed: August 22, 2012
    Date of Patent: August 4, 2015
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Abraham P. Lee, Andrew C. Hatch
  • Patent number: 9029297
    Abstract: The invention provides a method of printing, onto a substrate (12), an array (14) of spots of reagent compositions for use in a chemical and/or biochemical analysis. The method includes displacing an array of reagent composition containing capillary tubes (22) arranged alongside one another from an inoperative position to an operative position in which open ends of the capillary tubes (22) simultaneously impinge against a substrate and thereafter displacing the array of tubes (22) from the operative position back to the inoperative position. The invention extends to a printing apparatus (10), a method of printing a layered array of spots of reagent compositions, a method of introducing reagent compositions into the tubes, a reagent introducing device for introducing reagent compositions into the tubes and a printing installation which includes the printing apparatus (10) and the reagent introducing device.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: May 12, 2015
    Assignee: CSIR
    Inventor: Neil Emans
  • Publication number: 20150126378
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.
    Type: Application
    Filed: October 22, 2014
    Publication date: May 7, 2015
    Inventors: JONATHAN M. ROTHBERG, WOLFGANG HINZ, KIM L. JOHNSON, JAMES BUSTILLO, JOHN LEAMON, JONATHAN SCHULTZ
  • Publication number: 20150119274
    Abstract: The present invention describes microplates with arrays of wells containing magnetic inserts in the form of disks, cylinders or otherwise shaped materials with apertures in the centre for optical interrogation of the contacting solution. These inserts are capable of capturing ferro- and paramagnetic nano- and microparticles. The subject microplates find use in a variety of applications, including clinical and environmental assays, high throughput screening for genomics, proteomics and pharmaceutical applications, point-of-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally and high-throughput screening of materials for separation and catalysis.
    Type: Application
    Filed: June 3, 2013
    Publication date: April 30, 2015
    Inventors: Sergey Anatoliyovich Piletsky, Olena Piletska, Antonio Guerreiro, Alessandro Poma, Khalku Karim, Stanislav Piletsky
  • Patent number: 8999642
    Abstract: The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
    Type: Grant
    Filed: August 25, 2009
    Date of Patent: April 7, 2015
    Assignee: Illumina, Inc.
    Inventors: Andrea Sabot, Roberto Rigatti, Min-Jui Richard Shen
  • Patent number: 8999726
    Abstract: Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells, microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on, a separate surface. Each orifice is connected to two wells via microconduits. When the spotter contacts a surface, a seal is formed between the orifices and the surface. The same or different substances may be flowed across each orifice. Any number of orifices may be incorporated into a spotter.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: April 7, 2015
    Assignee: University of Utah Research Foundation
    Inventors: David A. Chang-Yen, Sriram Natarajan, Josh Eckman, Bruce K. Gale, David Myszka
  • Publication number: 20150094238
    Abstract: A system for inserting reagent or macrobeads into bores of a sample plate is disclosed comprising an insertion device arranged to retain one or more reagent or macrobeads by means of a vacuum whilst inserting the reagent or macrobeads into bores of the sample plate.
    Type: Application
    Filed: March 6, 2013
    Publication date: April 2, 2015
    Applicant: Dynex Technologies, Inc.
    Inventors: Adrian Bunce, Andrew Fusellier
  • Publication number: 20150087558
    Abstract: A method of making an assay device comprising providing micro-elements in the form of micro-particles or micro-length tube detection elements and thereafter with an automated tool, picking and placing the micro-elements into open-sided microfluidic channels in a body.
    Type: Application
    Filed: September 6, 2014
    Publication date: March 26, 2015
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 8975215
    Abstract: The present invention relates to methods for producing an oligonucleotide on a solid substrate surface and methods for using the same. Some aspects of the invention provide methods for selecting a single DNA molecule reproducibily with an atomic force microscope (AFM).
    Type: Grant
    Filed: November 16, 2009
    Date of Patent: March 10, 2015
    Assignees: Postech Foundation, Posco
    Inventors: Joon Won Park, Bong Jin Hong, Duk Hoe Kim
  • Patent number: 8969257
    Abstract: A reactor assembly having a plurality of reaction chambers defined therein is provided. The reactor assembly includes a fluid flow module that provides a pressurized control flow of fluid from an open container. In another embodiment, the reactor block includes a plurality of passageways defined over a surface of a substrate to accommodate the combinatorial processing in order to obtain multiple data points from a single substrate.
    Type: Grant
    Filed: March 6, 2008
    Date of Patent: March 3, 2015
    Assignee: Intermolecular, Inc.
    Inventors: Zachary Fresco, Rich Endo
  • Publication number: 20150050278
    Abstract: Fc domains and CH3 domains are disclosed that bind the neonatal Fc (FcRn) receptor and are at least 99% monomeric. Monomeric Fc domain molecules and CH3 domain molecules are disclosed herein that include a monomeric Fc domain or a monomeric CH3 domain and an effector molecule. In some embodiments, the monomeric Fc or monomeric CH3 domain include amino acid substitutions and/or CDR insertions in the beta strands such that they specifically bind an antigen. Methods for using these monomeric Fc domains, monomeric CH3 domains, monomeric Fc domain molecules and CH3 domain molecules are also provided.
    Type: Application
    Filed: March 14, 2013
    Publication date: February 19, 2015
    Inventors: Dimiter S. Dimitrov, Tianlei Ying
  • Patent number: 8951567
    Abstract: Discrete microstructures of predefined size and shape are prepared using sol-gel phase-reversible hydrogel templates. An aqueous solution of hydrogel-forming material is covered onto a microfabricated silicon wafer master template having predefined microfeatures, such as pillars. A hydrogel template is formed, usually by lowering the temperature, and the formed hydrogel template is peeled away from the silicon master template. The wells of predefined size and shape on the hydrogel template are filled with a solution or a paste of a water-insoluble polymer, and the solvent is removed to form solid structures. The formed microstructures are released from the hydrogel template by simply melting the hydrogel template in water. The microstructures are collected by centrifugation. The microstructures fabricated by this method exhibit pre-defined size and shape that exactly correspond to the microwells of the hydrogel template.
    Type: Grant
    Filed: September 27, 2008
    Date of Patent: February 10, 2015
    Assignee: Akina, Inc.
    Inventors: Kinam Park, Ghanashyam Acharya, Haesun Park
  • Publication number: 20150038377
    Abstract: A liquid dispenser for a microfluidic assay system is described. The dispenser includes at least one transfer pin for transferring a microfluidic sample of liquid to a target receptacle. A pin tip at one end of the transfer pin is structured to cooperate with an opening in the target receptacle. The tip uses a high voltage potential to transfer the sample from the pin to the receptacle.
    Type: Application
    Filed: July 15, 2014
    Publication date: February 5, 2015
    Inventors: Leila Madrone, John Linton, Colin John Herbert Brenan
  • Publication number: 20150031558
    Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.
    Type: Application
    Filed: October 15, 2014
    Publication date: January 29, 2015
    Inventors: Todd REARICK, Jessica Lynn REED, Jason GIOIA, Devin DRESSMAN, Nicholas HAPSHE, Brian REED, John Andrew SHERIDAN
  • Publication number: 20150018243
    Abstract: To provide a means and a method of quickly sampling a tissue fragment from a plant tissue, quickly preserving a gene expression state within the tissue fragment, and comprehensively analyzing the gene expression state. The present invention relates to a method of sampling a plant tissue section, comprising: inserting a first gel layer into a needle; arranging a plant tissue on a second gel layer; and passing the needle through the plant tissue together with the second gel layer, and sampling a section of the plant tissue in the needle.
    Type: Application
    Filed: December 18, 2012
    Publication date: January 15, 2015
    Inventors: Tomoharu Kajiyama, Hideki Kambara, Toru Habu
  • Patent number: 8932879
    Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: January 13, 2015
    Assignee: Ambergen, Inc.
    Inventors: Mark J. Lim, Kenneth J. Rothschild
  • Publication number: 20150011425
    Abstract: The present invention relates to methods and arrays for use in high resolution imaging of individual nucleic acid molecules and chromatin fragments, including native chromatin fragments. In one aspect, the present invention relates to a chromatin array that includes a transfer platform having a support and a transfer surface layered on the support. The chromatin array also includes a plurality of elongated individual native chromatin fragments coupled to the transfer surface in an orderly pattern suitable for high resolution imaging of the plurality of native chromatin fragments. The native chromatin fragments of the chromatin array include both DNA and histones.
    Type: Application
    Filed: January 28, 2013
    Publication date: January 8, 2015
    Applicant: CORNELL UNIVERSITY
    Inventors: Aline Cerf, Harold G. Craighead, Harvey C. Tian
  • Publication number: 20150011435
    Abstract: Methods and compositions for determining the nucleic acid sequence of polynucleotides that are at least 1500 nucleotides in length are provided.
    Type: Application
    Filed: September 26, 2014
    Publication date: January 8, 2015
    Inventors: Chao-ting WU, George M. CHURCH
  • Patent number: 8927465
    Abstract: A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray.
    Type: Grant
    Filed: August 3, 2007
    Date of Patent: January 6, 2015
    Assignee: National University University of Singapore
    Inventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson Ng
  • Publication number: 20140342935
    Abstract: The present invention is to provide a base for use in the amplification of a nucleic acid, which makes it possible to accurately and effectively amplify and detect a plurality of target nucleic acid sequences that are subjects for detection. A base for use in the amplification of a nucleic acid according to the present invention is a base in which a hydrophilic gel is held and which has arrayed plural zones, and the zones contain different kinds of primer or the like for amplifying nucleic acids.
    Type: Application
    Filed: November 27, 2012
    Publication date: November 20, 2014
    Applicant: Mitsubishi Rayon Co., Ltd.
    Inventor: Hiroyuki Ooshima
  • Publication number: 20140336072
    Abstract: A platform for biological assays includes a base substrate providing structural support to the platform, at least one surface of the base substrate coated with position markers, a first deformable layer positioned on top of the base substrate, and a second deformable layer positioned on top of the first deformable layer, the second deformable layer embedded with deformation markers.
    Type: Application
    Filed: November 16, 2012
    Publication date: November 13, 2014
    Inventors: Ramaswamy Krishnan, Allen Ehrlicher, James Butler, David A. Weitz, Jeffrey J. Fredberg, Chan Young Park
  • Publication number: 20140329721
    Abstract: Described herein are kits and components thereof used for a multiplexed analysis of a set of cytokines.
    Type: Application
    Filed: January 2, 2014
    Publication date: November 6, 2014
    Applicant: MESO SCALE TECHNOLOGIES, LLC
    Inventors: John Joern, Joseph Manimala, Keith McClary, Pankaj Oberoi, Gisbert Spieles, David Stewart, James Wilbur
  • Publication number: 20140329722
    Abstract: Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.
    Type: Application
    Filed: July 15, 2014
    Publication date: November 6, 2014
    Applicant: SANDIA CORPORATION
    Inventors: Thomas D. Perroud, Kamlesh D. Patel
  • Publication number: 20140323351
    Abstract: The present invention relates to single cell array micro-chips and fabrication, electrical measurement and electroporation method thereof. The single cell array microchip comprises a substrate (1), a plurality of positioning electrodes (2) formed in an array, a plurality of measuring electrode-pairs (3) formed in an array, and a micro sample pool (4). The invention integrates cell array positioning with electrical measurement and electroporation for living cells, which is characteristic of label-free and noninvasive methods to manipulate, position particles/cells as well as further measure their electrical parameters. Therefore, single-cell-array positioning and multi-mode in-situ real-time measurement can be realized for intensive analysis. Since the positioned cells are immobile, the precision of the electrical measurement of cells is effectively improved, so is the efficiency of electroporation with lower cell mortality rate.
    Type: Application
    Filed: July 10, 2014
    Publication date: October 30, 2014
    Inventors: Rong ZHU, Xiaoliang GUO
  • Patent number: 8871688
    Abstract: The present invention relates to a method for absolute quantification of polypeptides.
    Type: Grant
    Filed: June 2, 2008
    Date of Patent: October 28, 2014
    Assignees: INSERM (Institut National de la Sante et de la Recherche Medicale), Commissariat a l'Energie Atomique
    Inventors: Virginie Brun, Alain Dupuis, Jerome Garin
  • Publication number: 20140303010
    Abstract: A method of preparing a species-specific phosphorylation site peptide array for a target organism comprising: a) selecting a plurality of known non-target organism (NTO) phosphorylation site sequences and cognate known NTO phosphorylation polypeptide sequences from one or more NTO, each of the known NTO phosphorylation site sequences comprising at least 5 residues and less than 30 residues; b) identifying a matching target organism (TO) phosphorylation site sequence and cognate TO phosphorylation polypeptide sequence for one or more of the known NTO phosphorylation site sequences; c) determining the matching TO phosphorylation site sequences that correspond to orthologue polypeptides of the cognate known NTO phosphorylation polypeptide sequences; d) selecting the matching TO phosphorylation site sequences determined to correspond to orthologue polypeptides for inclusion on the array; wherein the matching TO phosphorylation site sequences that correspond to orthologue polypeptides are determined by calculating
    Type: Application
    Filed: September 21, 2012
    Publication date: October 9, 2014
    Inventors: Brett Trost, Anthony Kusalik, Scott Napper, Ryan Arsenault, Philip Griebel
  • Publication number: 20140303009
    Abstract: The subject matter relates to relates to a one-bead-one-sequence composition, a library of tagged chemicals comprising a plurality of one-bead-one-sequence compositions, a method for identifying a candidate molecule from a library of tagged chemicals, and a composition produced by a process, all as described herein.
    Type: Application
    Filed: April 23, 2014
    Publication date: October 9, 2014
    Applicant: Wake Forest University
    Inventors: Martin Guthold, Jed C. Macosko
  • Publication number: 20140296087
    Abstract: The present application pertains to products and methods related to the ability of short nucleotide oligomers to bind the tertiary or globular structure of nucleic acids. This application discloses libraries of short oligomers and methods for using these libraries.
    Type: Application
    Filed: October 25, 2013
    Publication date: October 2, 2014
    Applicant: President and Fellows of Harvard College
    Inventors: Gregory L. Verdine, Webster Santos
  • Patent number: 8843279
    Abstract: An apparatus, method and sensor apparatus for determining a spatial positioning of loading equipment is disclosed. The loading equipment has an operating implement for loading a payload, the operating implement being coupled to a support for movement relative to the support. The apparatus includes an orientation sensor disposed on the support and being operable to produce an orientation signal representing an orientation of the support. The apparatus also includes a displacement sensor operable to produce a displacement signal representing a displacement of the operating implement relative to the support. The apparatus further includes a processor circuit operably configured to receive the orientation signal and the displacement signal, use a kinematic model of the loading equipment to compute a spatial positioning of the loading equipment, and produce an output signal representing the spatial positioning.
    Type: Grant
    Filed: June 6, 2011
    Date of Patent: September 23, 2014
    Assignee: Motion Metrics International Corp.
    Inventors: Shahram Tafazoli Bilandi, Amir Mohammad Ahani, Abdol Rasul Rasuli
  • Publication number: 20140271614
    Abstract: The instant invention comprises a process for the solid phase synthesis of directed epitope peptide mixtures useful in the treatment and diagnosis of protein conformational disorders, such process defined by a set of rules regarding the identity and the frequency of occurrence of amino acids that substitute a base or native amino acid of a known epitope. The resulting composition is a mixture of related peptides for therapeutic use. The invention also pertains to the process of generating antibodies using the directed epitope peptide mixtures as the antigens, and antibodies generated by such process, useful in the treatment and diagnostics of the said protein conformational disorder.
    Type: Application
    Filed: September 12, 2013
    Publication date: September 18, 2014
    Applicant: DECLION PHARMACEUTICALS, INC.
    Inventors: Dustan Bonnin, Eric Zanelli, Thomas P. Mathers
  • Publication number: 20140274762
    Abstract: The present invention provides kits and assay methods for the early detection of pathogens, precise identification of the etiologic agent, and improved disease surveillance. More specifically, the present invention discloses an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres), said support being previously coated with an AGT substrate. This coupling is mediated by the irreversible reaction of the AGT enzyme on its substrate. The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures.
    Type: Application
    Filed: December 10, 2012
    Publication date: September 18, 2014
    Inventors: Jean-Claude Manuguerra, Jessica Vanhomwegen, Philippe Despres, Sylvie Paulous
  • Publication number: 20140235510
    Abstract: Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells, microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on, a separate surface. Each orifice is connected to two wells via microconduits. When the spotter contacts a surface, a seal is formed between the orifices and the surface. The same or different substances may be flowed across each orifice. Any number of orifices may be incorporated into a spotter.
    Type: Application
    Filed: February 25, 2013
    Publication date: August 21, 2014
    Applicant: University of Utah Research Foundation
    Inventors: David A. Chang-Yen, Sriram Natarajan, Josh Eckman, Bruce K. Gale, David Myszka
  • Publication number: 20140228235
    Abstract: The present invention relates to a microarray device for the screening or the finding of protein inhibitors, to a method for the production thereof, and to a corresponding method for screening or finding protein inhibitors. The microarray device according to the invention comprises a solid supporting element having a support material, at least one protein immobilized thereon for which inhibitors are to be screened or found, and at least one known inhibitor of the at least one protein, the inhibitor being bound to the at least one protein and comprising a detectable label.
    Type: Application
    Filed: April 20, 2012
    Publication date: August 14, 2014
    Inventors: Dieter Melzner, Thomas Scheper, Thomas Schuler, Frank Stahl, Denise Van Rossum, Johanna-Gabriela Walter, Carsten Zeilinger
  • Patent number: 8802024
    Abstract: A method for manufacturing a biochip is provided. First, a first self-assembled monolayer is coated on a substrate. Next, a plurality of first biomedical molecular dots are formed on the first self-assembled monolayer by micro-titration technique. After the bonding reaction between the first biomedical molecular point and the first self-assembled monolayer, a second self-assembled monolayer is deposited on the surface of the first self-assembled monolayer by evaporation. The second self-assembled monolayer attached on the plurality of first biomedical molecular dots and the first self-assembled monolayer not bonded to the substrate are removed, so that the first biomedical molecular dots immobilized on the first self-assembled monolayer are exposed. Finally, a second biomedical molecular layer is immobilized on the exposed portions of the first biomedical molecular dots.
    Type: Grant
    Filed: September 28, 2007
    Date of Patent: August 12, 2014
    Assignee: National Tsing Hua University
    Inventors: Fan Gang Tseng, Hsin Yi Hsieh, Chun Lung Wu
  • Publication number: 20140200158
    Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
    Type: Application
    Filed: February 28, 2014
    Publication date: July 17, 2014
    Applicant: Illumina, Inc.
    Inventors: M. Shane Bowen, Kevin L. Gunderson, Shengrong Lin, Maria Candelaria Rogert Bacigalupo, Kandaswamy Vijayan, Yir-Shyuan Wu, Bala Murali Venkatesan, James Tsay, John M. Beierle, Lorenzo Berti, Sang Ryul Park
  • Patent number: 8778849
    Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: July 15, 2014
    Assignee: Illumina, Inc.
    Inventors: M. Shane Bowen, Kevin L. Gunderson, Shengrong Lin, Maria Candelaria Rogert Bacigalupo, Kandaswamy Vijayan, Yir-Shyuan Wu, Bala Murali Venkatesan, James Tsay, John M. Beierle, Lorenzo Berti, Sang Ryul Park
  • Patent number: 8759259
    Abstract: Embodiments of the invention are directed to a one-bead-two-compound method for the creation of encoded cyclic peptoid libraries. This scheme is useful for the creation of cyclic peptoid microarrays since only the cyclic peptoid, not the linear encoding molecule, contains an attachment residue and thus can be spotted onto an activated substrate.
    Type: Grant
    Filed: October 15, 2010
    Date of Patent: June 24, 2014
    Assignee: The Board of Regents of the University of Texas System
    Inventors: Yong-Uk Kwon, Thomas Kodadek
  • Publication number: 20140155297
    Abstract: A method of retrieving sequence-verified deoxyribonucleic acid (DNA) from a DNA sequencing plate includes directing a laser beam to impinge on a predetermined area on the DNA sequencing plate. The DNA sequencing plate contains a plurality of sequence-verified DNA disposed thereon. The method also includes exposing the predetermined area to a radiation dosage provided by the laser beam to generate at least one of an optical pressure or an ablating force in the predetermined area. The optical pressure or ablating force result in the ejection of at least one sequence-verified DNA disposed proximate to the predetermined area.
    Type: Application
    Filed: February 25, 2013
    Publication date: June 5, 2014
    Applicant: CAMBRIAN GENOMICS, INC.
    Inventor: Austen Heinz
  • Publication number: 20140155295
    Abstract: This disclosure provides microwell capsule array devices. The microwell capsule array devices are generally capable of performing one or more sample preparation operations. Such sample preparation operations may be used as a prelude to one more or more analysis operations. For example, a device of this disclosure can achieve physical partitioning and discrete mixing of samples with unique molecular identifiers within a single unit in preparation for various analysis operations. The device may be useful in a variety of applications and most notably nucleic-acid-based sequencing, detection and quantification of gene expression and single-cell analysis.
    Type: Application
    Filed: August 13, 2013
    Publication date: June 5, 2014
    Applicant: 10X Technologies, Inc.
    Inventors: Benjamin Hindson, Serge Saxonov
  • Publication number: 20140121132
    Abstract: The present invention relates to a system for producing a nucleic acid molecule imaging array for use in high resolution imaging of individual nucleic acid molecules. The system includes a micro/nanostructured capture array having a hydrophobic surface having topographical features effective to assist in capillary-based trapping and elongation of individual nucleic acid molecules. The system also includes a transfer platform having a support and a hydrophobic substrate layered on the support. The transfer platform is effective to receive and capture, through solvent mediation, the trapped and elongated individual nucleic acid molecules from the micro/nanostructured capture array. The present invention also relates to a nucleic acid molecule imaging array, a transfer platform for use in preparing a nucleic acid molecule array, and a kit for producing a nucleic acid molecule imaging array for use in high resolution imaging of individual nucleic acid molecules.
    Type: Application
    Filed: March 12, 2012
    Publication date: May 1, 2014
    Applicant: CORNELL UNIVERSITY
    Inventors: Aline Cerf, Harold G. Craighead, Thomas Alava
  • Publication number: 20140106980
    Abstract: A sensor having a substrate is provided in which structures are disposed on a surface of the substrate. The structures can be, e.g., nanostructures. Polarized light is directed toward the sensor, and birefringence of the structures with respect to the light is measured. Target particles that interact with the structures are detected based on changes in the measured birefringence.
    Type: Application
    Filed: October 15, 2013
    Publication date: April 17, 2014
    Inventors: Mathias M. Schubert, Tino Hofmann, Daniel Schmidt, Patrick H. Dussault, Andrea Holmes, Rebecca Y. Lai
  • Publication number: 20140100122
    Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.
    Type: Application
    Filed: October 18, 2013
    Publication date: April 10, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Todd REARICK, Jessica Lynn REED, Jason GIOIA, Ph.D., Devin DRESSMAN, Nicholas HAPSHE, Brian REED, John Andrew SHERIDAN
  • Patent number: 8691151
    Abstract: This invention provides an apparatus for particle sorting, particle patterning, and methods of using the same. The sorting or patterning is opto-fluidics based, in that particles are applied to individual chambers in the device, detection and/or analysis of the particles is carried out, such that a cell or population whose removal or conveyance is desired is defined, and the cell or population is removed or conveyed via application of an optical force and flow-mediated conveyance or removal of the part.
    Type: Grant
    Filed: August 27, 2007
    Date of Patent: April 8, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Joseph Kovac, Joel Voldman
  • Publication number: 20140094383
    Abstract: Disclosed are compositions and methods for the use of lipoplex nanoparticle chips and arrays in the detection of/diagnosis of a disease or condition.
    Type: Application
    Filed: October 2, 2013
    Publication date: April 3, 2014
    Inventors: Ly James Lee, Kwang Joo Kwak, Bo Yu, Yun Wu, Andrew Lee
  • Publication number: 20140087971
    Abstract: Analysis of a system and/or sample involves the use of absorption-encoded micro beads. Each type of micro bead is encoded with amounts of the k dyes in a proportional relationship that is different from proportional relationships of the k dyes of others of the n types of absorption-encoded micro beads. A system and/or a sample can be analyzed using information obtained from detecting the one or more types of absorption-encoded micro beads.
    Type: Application
    Filed: September 26, 2012
    Publication date: March 27, 2014
    Applicant: PALO ALTO RESEARCH CENTER INCORPORATED
    Inventors: Peter Kiesel, Joerg Martini, Michael I. Recht
  • Patent number: 8673215
    Abstract: The invention relates to the test device for platelet aggregation detection comprising: —an element (1) for receiving a blood sample—a capillary tube (3) connected at a first end (31) to said element (1) and at a second end (32) to a pressure lowering device (5) to pump said blood sample through said capillary tube (3)—at least a pair of facing electrodes (8) on the capillary tube—a device for measuring an impedance between said pair of facing electrodes. The invention also relates to a process for using this device, comprising: a) receiving a blood sample and pumping it through the capillar tube (3) b) determining a dynamic change of the value of the impedance between at least one pair of electrodes (8).
    Type: Grant
    Filed: October 16, 2008
    Date of Patent: March 18, 2014
    Assignee: Centre National de la Recherche Scientifique
    Inventors: Francine Rendu, Daniel Fruman, Jaime Levenson
  • Publication number: 20140066318
    Abstract: The present invention relates to methods and products for the localized or spatial detection of nucleic acid in a tissue sample and in particular to a method for localized detection of nucleic acid in a tissue sample comprising: (a) providing an array comprising a substrate on which multiple species of capture probes are directly or indirectly immobilized such that each species occupies a distinct position on the array and is oriented to have a free 3? end to enable said probe to function as a primer for a primer extension or ligation reaction, wherein each species of said capture probe comprises a nucleic acid molecule with 5? to 3?: (i) a positional domain that corresponds to the position of the capture probe on the array, and (ii) a capture domain; (b) contacting said array with a tissue sample such that the position of a capture probe on the array may be correlated with a position in the tissue sample and allowing nucleic acid of the tissue sample to hybridize to the capture domain in said capture probes;
    Type: Application
    Filed: March 13, 2012
    Publication date: March 6, 2014
    Applicant: SPATIAL TRANSCRIPTOMICS AB
    Inventors: Jonas Frisen, Patrik Ståhl, Joakim Lundeberg