By Measuring The Ability To Specifically Bind A Target Molecule (e.g., Antibody-antigen Binding, Receptor-ligand Binding, Etc.) Patents (Class 506/9)
-
Patent number: 11603563Abstract: Provided is a method of predicting differentiation potential of undifferentiated iPS cells into cartilage cells. Provided is a genetic marker for predicting differentiation potential of undifferentiated iPS cells into cartilage cells. A method of predicting differentiation potential of undifferentiated iPS cells into cartilage cells based on gene expression data of the undifferentiated iPS cells. A method of predicting differentiation potential of undifferentiated iPS cells into cartilage cells by predicting differentiation potential of the iPS cells into neural crest cells (NC cells) based on gene expression data of the undifferentiated iPS cells.Type: GrantFiled: June 8, 2018Date of Patent: March 14, 2023Assignees: Shimadzu Corporation, Kyoto UniversityInventors: Makoto Watanabe, Taka-Aki Sato, Junya Toguchida
-
Patent number: 11597744Abstract: Disclosed are chiral phosphoramidite auxiliary compositions for diastereoselective synthesis of stereochemically enriched oligonucleotides, and nucleoside phosphoramidite compounds comprising the same. Exemplary structures of the chiral phosphoramidite auxiliary compositions include Formulas (IA) and (IB).Type: GrantFiled: July 2, 2018Date of Patent: March 7, 2023Assignee: SIRIUS THERAPEUTICS, INC.Inventors: Sukumar Sakamuri, Curt W. Bradshaw, Dingguo Liu, Laxman Eltepu
-
Patent number: 11598779Abstract: Methods for identifying and detecting potential disease specific biomarkers from biofluids. The methods involve in vivo administration of nanoparticles to a subject in a diseased state or incubating nanoparticles in a biofluid sample taken from a subject in a diseased state and analysis of the biomolecule corona formed on said nanoparticles. The methods distinguish between a healthy and diseased state in a subject, such as, for example, the presence of a tumor in a human subject.Type: GrantFiled: September 6, 2017Date of Patent: March 7, 2023Assignee: THE UNIVERSITY OF MANCHESTERInventors: Kostas Kostarelos, Marilena Hadjidemetriou
-
Patent number: 11579144Abstract: Methods and systems for identifying a protein within a sample are provided herein. A panel of antibodies are acquired, none of which are specific for a single protein or family of proteins. Additionally, the binding properties of the antibodies in the panel are determined. Further, the protein is iteratively exposed to a panel of antibodies. Additionally, a set of antibodies which bind the protein are determined. The identity of the protein is determined using one or more deconvolution methods based on the known binding properties of the antibodies to match the set of antibodies to a sequence of a protein.Type: GrantFiled: March 3, 2021Date of Patent: February 14, 2023Assignee: NAUTILUS BIOTECHNOLOGY, INC.Inventor: Parag Mallick
-
Patent number: 11572563Abstract: A method of capturing human immunoglobulin Fc domains in a biofluid sample is provided. The method includes providing an affinity capture device. The affinity capture device includes a surface having an aptamer that is at least 80% identical to SEQ ID NO 1 immobilized onto the surface of the affinity capture device. The biofluid sample is diluted with a binding buffer. The binding buffer includes (A) tris(hydroxymethyl)aminomethane (Tris), trimethylamine (TES), 2-ethanesulfonic acid (MES), or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES); (B) a magnesium cation at a concentration between about 10 ?M to about 20 mM; and (C) a total monovalent cation concentration from 0 to no greater than 100 mM. The human immunoglobulin Fc domains in the biofluid sample are adsorbed to the aptamer with the binding buffer.Type: GrantFiled: June 26, 2020Date of Patent: February 7, 2023Assignee: Waters Technologies CorporationInventors: Xiaoxiao Liu, Matthew A. Lauber
-
Patent number: 11572588Abstract: The present invention provides novel markers of treatment response in a subject infected with Mycobacterium, which allow for quantifying treatment impact on the physiologic state of the Mycobacterium.Type: GrantFiled: July 19, 2018Date of Patent: February 7, 2023Assignees: The Regents of the University of Colorado, a body corporate, The Regents of the University of California, Yale University, Colorado State University Research Foundation, The Board of Trustees of the Leland Standford Junior UniversityInventors: Nicholas D. Walter, Martin Voskuil, Gary Schoolnik, Gregory Dolganov, J. Lucian Davis, Payam Nahid, Greg Robertson, Anne Lenaerts
-
Patent number: 11566292Abstract: There is described herein a method of prognosing endocrine-only treatment in a subject with breast cancer, the method comprising: a) providing a tumor sample of the breast cancer; b) determining the expression level of at least 40 of the genes listed in Table 4 in the tumor sample; c) comparing said expression levels to a reference expression level of the group of genes from control samples from a cohort of subjects; and d) determining the residual risk associated with the breast cancer; wherein a statistically significant difference or similarity in the expression of the group of genes compared to the reference expression level corresponds to a residual risk associated with breast cancer.Type: GrantFiled: December 7, 2016Date of Patent: January 31, 2023Assignee: ONTARIO INSTITUTE FOR CANCER RESEARCH (OICR)Inventors: Jane Bayani, John M. S. Bartlett, Cindy Q. Yao, Paul C. Boutros
-
Patent number: 11561222Abstract: The present invention relates to a method for diagnosis of bile duct cancer, using methionyl-tRNA synthetase (MRS) in bile duct cells of a latent patient.Type: GrantFiled: November 11, 2019Date of Patent: January 24, 2023Assignee: ONCOTAG DIAGNOSTICS CO., LTD.Inventors: Sunghoon Kim, Nam Hoon Kwon, Dong Ki Lee, Beom Jin Lim, Sung Ill Jang
-
Patent number: 11549152Abstract: Products, systems, and methods for classifying human colorectal cancer into a consensus molecular subtype (CMS) and for assessing risk of recurrence based on CMS scores and based on risk scores derived from abbreviated gene expression profiles, for determining suitable treatment protocols for human colorectal cancer patients based on the determined CMS classification and based on the determined risk of recurrence, and for administering the suitable treatment protocols.Type: GrantFiled: March 7, 2019Date of Patent: January 10, 2023Assignee: University of Notre Dame du LacInventor: Steven Buechler
-
Patent number: 11544350Abstract: A Fisher's exact test calculation apparatus includes: a condition storage 1 that has stored therein a condition for determining whether a result of Fisher's exact test corresponding to input is significant or not, the input being frequencies in a summary table; and a calculation unit 2 that obtains the result of Fisher's exact test corresponding to the frequencies in the summary table by inputting the frequencies in the summary table to the condition read from the condition storage 1.Type: GrantFiled: June 30, 2017Date of Patent: January 3, 2023Assignees: NIPPON TELEGRAPH AND TELEPHONE CORPORATION, TOHOKU UNIVERSITYInventors: Koji Chida, Satoshi Hasegawa, Koki Hamada, Masao Nagasaki, Kazuharu Misawa
-
Patent number: 11542557Abstract: Provided herein is technology for colorectal neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of colorectal neoplasia in 1) individuals at, older or younger than 50 years of age, or 2) individuals having Lynch Syndrome.Type: GrantFiled: July 18, 2019Date of Patent: January 3, 2023Assignee: Mayo Foundation for Medical Education and ResearchInventors: David A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
-
Patent number: 11535893Abstract: The invention provides a method of determining the epigenetic chromosome interactions which are relevant to an epigenetic test for a neurodegenerative condition.Type: GrantFiled: June 19, 2019Date of Patent: December 27, 2022Assignee: Oxford BioDynamics PLCInventors: Aroul Selvam Ramadass, Ewan Hunter, Alexandre Akoulitchev
-
Patent number: 11530451Abstract: Disclosed herein are methods for a RNA in situ hybridization assay workflow for the detection of target RNA within intact cells for the detection of prostate cancer cells in urine samples. The methods disclosed herein can identify a genetic susceptibility to prostate cancer in a subject and differentiate high risk from low risk prostate cancers. The methods disclosed herein can also include treatment and management strategies for prostate cancer and the prevention thereof.Type: GrantFiled: March 1, 2019Date of Patent: December 20, 2022Assignee: THE JOHNS HOPKINS UNIVERSITYInventors: Jun Luo, Christian P. Pavlovich, William Brewster Isaacs, Jillian N. Eskra
-
Patent number: 11519845Abstract: The present invention relates to a device (10) for use in fluid sample analysis. It is described to position (310) a top part (20) of the device (10) adjacent to a base part (30) of the device so as to define a fluidic receiving region in between, the top part being provided with a through opening fluidly connected to the fluidic receiving region, and the bottom part being provided with a radiation window adjacent to the fluidic receiving region. A fluidic sample is supplied (320) through the opening (24). The fluidic sample is moved laterally (330) in the fluid receiving region without the use of an intermediary membrane between the top part and the base part. A radiation is emitted (340) to the fluid receiving region. A radiation is detected (350) that is reflected by the device. A presence of the fluidic sample is determined (360) on the basis of a measured reflectance value based on the detected radiation.Type: GrantFiled: May 16, 2017Date of Patent: December 6, 2022Assignee: Siemens Healthineers Nederland B.V.Inventors: Alexander Van Reenen, Markus Hendrikus Van Roosmalen, Hendrik Sibolt Van Damme
-
Patent number: 11513123Abstract: This invention provides methods, reagents, and diagnostic and prognostic markers useful for minimally invasive identification, diagnosis, and therapeutic intervention in individuals with colorectal cancers, or individuals who may be susceptible to developing colorectal cancers.Type: GrantFiled: December 10, 2015Date of Patent: November 29, 2022Assignee: Wisconsin Alumni Research FoundationInventors: Melanie Mae Ivancic, Michael Richard Sussman, William Franklin Dove, Amy Ann Irving, Jennifer Kathleen Pleiman, Edward Lee Huttlin, Xiaodi Chen, Adrian Daniel Hegeman, Mark Reichelderfer, Gregory D. Kennedy, Perry J. Pickhardt
-
Patent number: 11505826Abstract: The present disclosure is directed to a single-end sequencing method for improved detection of genomic rearrangements such as deletions, insertions, inversions, and translocations that are present in a polynucleotide. A first priming event allows for sequencing of a target sequence, and a second priming event on an adapter allows for identification of the sequences amplified and tagged by selective amplification. The combination of priming events in the same direction facilitates read alignment and the identification of any genomic rearrangements.Type: GrantFiled: July 12, 2017Date of Patent: November 22, 2022Assignee: Agilent Technologies, Inc.Inventors: Margherita Corioni, Douglas N Roberts
-
Patent number: 11499196Abstract: Disclosed herein are methods and systems of utilizing sequencing reads for detecting and quantifying the presence of a tissue type or a disease type in cell-free DNA prepared from blood samples.Type: GrantFiled: June 7, 2017Date of Patent: November 15, 2022Assignees: The Regents of the University of California, University of Southern CaliforniaInventors: Xianghong Jasmine Zhou, Shuli Kang, Steven Dubinett, Wenyuan Li, Qingjiao Li
-
Patent number: 11491483Abstract: Devices for high throughput cell electroporation include a trapping component that at least partially defines an upper boundary of a microfluidic chamber. A cell trap array is patterned on the underside of the trapping component, and a channeling component is positioned beneath the trapping component. The channeling component includes a vertically oriented nanochannel array. The trapping component and the channeling component are positioned such that a given nanochannels is positioned beneath a cell trap. During use, fluid flow holds trapped cells in secure contact with the nanochannels beneath the cell trap. The device further includes upper and lower electrode layers for generating an electric field to electroporate trapped cells via the nanochannel array. A reservoir positioned beneath the channeling component can be filled transfection reagent solution. During electroporation, the transfection reagent solution travels through the nanochannel array during to transfect the trapped cells.Type: GrantFiled: February 15, 2019Date of Patent: November 8, 2022Assignee: Ohio State Innovation FoundationInventors: L. James Lee, Junfeng Shi
-
Patent number: 11492666Abstract: A method for determining the presence of an allele, including (a) binding a polymerase to a double stranded nucleic acid that includes a primer hybridized to a template, the template including a first allele of a locus; (b) adding a nucleotide to the primer via catalytic activity of the polymerase, thereby producing an extended nucleic acid; (c) dissociating the polymerase from the extended nucleic acid; (d) detecting dissociation of the polymerase from the extended nucleic acid; and (e) comparing the dissociation of the polymerase from the extended nucleic acid to dissociation of the polymerase from a second double stranded nucleic acid, the second double stranded nucleic acid including a primer hybridized to the same position of the locus as the primer of the extended nucleic acid.Type: GrantFiled: February 14, 2018Date of Patent: November 8, 2022Assignee: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.Inventors: Denis Malyshev, Sean Stromberg
-
Patent number: 11492602Abstract: The present invention provides a gene including an M13 p5 expressing cassette, which includes a promoter, a ribosome binding site (RBS) and a protein 5 (p5) coding region, wherein at least one base of sequences between the RBS and the p5 coding region is mutated. Using this gene may increase production of single-stranded DNA.Type: GrantFiled: December 3, 2019Date of Patent: November 8, 2022Assignee: Korea University Research and Business FoundationInventors: Min Kyu Oh, Dong June Ahn, Seungwoo Lee, Bo Young Lee, Jun Min Lee, Jae Won Lee
-
Patent number: 11493520Abstract: The invention relates to assays for nucleosome remodeling activity using functionalized recombinant mononucleosomes. The functionalized recombinant mononucleosomes comprise a histone octamer comprising recombinant histone H2A, H2B, H3 and H4 proteins and a DNA template comprising a nucleosome positioning sequence that effectively positions the histone octamer and a signal site. The invention further relates to methods of using the assay to quantify enzymatic activity of remodeling enzymes and identifying modulators of remodeling enzyme activity.Type: GrantFiled: May 18, 2018Date of Patent: November 8, 2022Assignee: EpiCypher, Inc.Inventors: Michael-Christopher Keogh, Martis William Cowles, Matthew Fruge Whelihan
-
Patent number: 11484581Abstract: Provided herein, are methods of treating cancer in dogs using personalized cancer vaccines comprising peptides having frameshift mutations caused by errors in transcription and splicing of an mRNA.Type: GrantFiled: June 1, 2018Date of Patent: November 1, 2022Assignee: Arizona Board of Regents on Behalf of Arizona State UniversityInventors: Stephen Albert Johnston, Luhui Shen
-
Patent number: 11479817Abstract: The present invention relates to a method for discriminating an operationally tolerant (TOL) subject from a non-operationally tolerant (STA) subject, comprising the following steps: i) establishing a composite score of tolerance (cSoT) with the expression levels of six genes in a biological sample obtained from said subject and two clinical parameters; wherein said six genes are ID3, AKR1C3, CD40, CTLA4, TCL1A and MZB1, and wherein said cSoT is established by the following formula: cSoT = ? i n ? = ? i × Exprs + ? test ? ? time × age test ? ? time + ? trans ? ? time × age trans ? ? time + intercept - scaling ? ? coefficient ii) comparing this cSoT with a predetermined reference value; and iii) concluding that the subject is TOL when the cSoT is higher than the predetermined reference value or concluding that the subject is STA when the cSoT is lower than the predetermined reference value.Type: GrantFiled: July 21, 2017Date of Patent: October 25, 2022Assignees: INSERM (Institut National de la Santé et de la Recherche Médicale, Université de Nantes, Centre Hospitalier Universitaire de Nantes, Centre National de la Recherche ScientifiqueInventors: Richard Danger, Sophie Brouard, Magali Giral, Gérard Ramstein
-
Patent number: 11474105Abstract: The present invention provides a method of diagnosing endometriosis and/or infertility in a subject, comprising: a) obtaining a sample from the subject; b) detecting a level of expression of a SIRT1 gene and/or protein in the sample; c) detecting a level of expression of a BCL6 gene and/or protein in the sample; d) comparing the level of expression detected in (b) with the level of expression of a SIRT1 gene and/or protein in a sample obtained from a control subject or a population of control subjects; e) comparing the level of expression detected in (c) with the level of expression of a BCL6 gene and/or protein in a sample obtained from a control subject or a population of control subjects; and f) diagnosing the subject as having infertility when the subject has a level of expression of the SIRT1 gene and/or protein greater than the level of expression of the SIRT1 gene and/or protein of the control subject or population of control subjects and also has a level of expression of the BCL6 gene and/or protein tType: GrantFiled: March 31, 2017Date of Patent: October 18, 2022Assignees: The University of North Carolina at Chapel Hill, Upstate Affiliate Organization, Board of Trustees of Michigan State UniversityInventors: Steven L. Young, Bruce Arthur Lessey, Jae Wook Jeong
-
Patent number: 11458995Abstract: Among other things, techniques are described for screening and monitoring the health of a vehicle user including receiving sensor data produced by a sensor at the vehicle, processing the sensor data to determine at least one health condition of the user of the vehicle, and in response to determining the at least one health condition, executing a vehicle function selected from a plurality of vehicle functions based on the at least one health condition.Type: GrantFiled: July 28, 2020Date of Patent: October 4, 2022Assignee: Motional AD LLCInventors: Eric Wolff, Abhimanyu Singh, Linh Pham
-
Patent number: 11458805Abstract: An air quality system for a vehicle includes a chemical detection apparatus including a plurality of nanofiber chemical sensors for sensing a plurality of airborne materials in a compartment of the vehicle. The plurality of nanofiber chemical sensors are configured to adjust a characteristic electrical signal in response to changes in the presence of the plurality of airborne materials. The chemical detection apparatus further includes a processor coupled to the nanofiber chemical sensor, wherein the processor is configured to monitor the characteristic electrical signals from the nanofiber chemical sensors and generate a detection signal in response to a change in the characteristic electrical signals. The processor is in communication with a controller configured to control at least one vehicle system in response to the detection of one or more of the airborne materials.Type: GrantFiled: December 18, 2019Date of Patent: October 4, 2022Assignee: GENTEX CORPORATIONInventors: Matthew W. Remijn, Darin D. Tuttle, Kurtis L. Geerlings, Douglas W. Later, Benjamin R. Bunes
-
Patent number: 11459623Abstract: The present invention relates to a method for detecting or quantifying deoxyribonucleic acid (DNA) of human immunodeficiency virus 2 (HIV-2) in a sample containing DNA comprising: a) performing a real-time polymerase chain reaction (PCR) on the sample, or a fraction thereof comprising DNA, with at least two sets of primers and probe each respectively comprising two primers and a labeled probe for the detection or quantification of HIV-2 DNA, at least one of the sets is selected from the group consisting of: a set comprising a primer comprising or consisting of a sequence SEQ ID NO: 1 or a sequence having al least 90% identity to SEQ ID NO: 1, a primer comprising or consisting of a sequence SEQ ID NO: 2 or a sequence having 90% identity to SEQ ID NO: 2 or the complement of these sequences, and a labeled probe comprising or consisting of a sequence SEQ ID NO: 3, or a sequence having at least 90% identify to SEQ ID NO: 3 or the complement of these sequences, and a set comprising a primer comprising or consistingType: GrantFiled: May 4, 2018Date of Patent: October 4, 2022Assignees: UNIVERSITÉ PARIS CITÉ, ASSISTANCE PUBLIQUE-HOPITAUX DE PARIS, CHU DE ROUEN, INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE, UNIVERSITE DE ROUEN, UNIVERSITÉ PARIS NORDInventors: Adeline Melard, Mélanie Bertine, Véronique Avettand Fenoel, Florence Damond, Christine Rouzioux, Diane Descamps, Marie Gueudin, Jean-Christophe Plantier
-
Patent number: 11458191Abstract: The present disclosure provides compositions and methods for targeting a Ras antigen to, for example, treat or prevent cancer. Disclosed embodiments include binding proteins, such as a T cell receptor or a chimeric antigen receptor, that bind to a Ras antigen:HLA complex. Polynucleotides encoding such binding protein can introduced into a host cell, such as a T cell, and the cell can be used in immunotherapy for treating various cancers. Also provided are immunogenic polypeptides that can be useful to, for example, induce an immune response against a mutated Ras or to identify a binding protein that binds to a Ras antigen.Type: GrantFiled: December 8, 2021Date of Patent: October 4, 2022Assignee: FRED HUTCHINSON CANCER CENTERInventors: Rachel Perret, Philip D. Greenberg, Thomas M. Schmitt, Aude G. Chapuis, Ingunn M. Stromnes, Tijana Martinov
-
Patent number: 11459602Abstract: The present invention relates to methods for the identification of methylated cytosines in a population of double stranded DNA molecules. The invention also relates to adapters and kits for synthesizing said adapters as well as to double stranded DNA libraries obtained by the methods of the invention.Type: GrantFiled: September 18, 2018Date of Patent: October 4, 2022Assignees: Fundadó Privada Institut De Medicina Predictiva I, Personalitzada Del Cáncer Llorenç Coll MuletInventors: Llorenç Coll Mulet, Miguel A. Peinado Morales
-
Patent number: 11459621Abstract: The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding corn plants having late wilt (LW) resistance using marker-assisted selection. The disclosure further provides corn germplasm resistant to LW. The disclosure also provides markers associated with LW resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel LW resistant germplasm.Type: GrantFiled: October 14, 2020Date of Patent: October 4, 2022Assignee: Monsanto Technology LLCInventors: Rakesh B, Sunil Kumar Biradar, Franck Jean Chopin, Romain Fouquet, Sonali Dilip Gandhi, Erappa Gangappa, Veeresh RP Gowda, Yule Pan, Jean-Luc Pellet, Dharanendra Swamy, Gonzalo Torres, Chongqing Xie
-
Patent number: 11453715Abstract: The present invention relates to methods for treating and preventing Staphylococcus aureus infection and/or a condition resulting from a S. aureus infection in a subject that involves administering compositions that inhibit S. aureus interaction with CXCR1/CXCR2 and DARC cellular receptors. The present invention further relates to novel compositions for carrying out these and other methods.Type: GrantFiled: June 5, 2020Date of Patent: September 27, 2022Assignee: NEW YORK UNIVERSITYInventors: Victor J. Torres, Tamara Reyes-Robles, Francis Alonzo, III
-
Patent number: 11453909Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.Type: GrantFiled: September 6, 2018Date of Patent: September 27, 2022Assignee: Life Technologies CorporationInventors: Stephen P. Hendricks, Michael Phelan, Marian Peris, Cheng-Yao Chen, Daniel Mazur, Xinzhan Peng, Amy Castillo
-
Patent number: 11453908Abstract: Disclosed herein are methods and compositions such as primers, primer pairs, and kits for typing KIR2DL1, KIR2DL2, Sand KIR2DL3 alleles. The compositions and methods disclosed herein are useful in the selection of most appropriate donors for HCT.Type: GrantFiled: September 28, 2017Date of Patent: September 27, 2022Assignee: MEMORIAL SLOAN KETTERING CANCER CENTERInventors: Katharine Hsu, Jean-Benoit Le Luduec
-
Patent number: 11441181Abstract: A use of at least one miRNA, said at least one miRNA being miR-124, as a biomarker, in particular of a viral infection, or of an efficacy of a therapeutic treatment of said viral infection.Type: GrantFiled: January 17, 2014Date of Patent: September 13, 2022Assignees: ABIVAX, CENTRE NATIONAL DE LA RECHERCHE, UNIVERSITE DE MONTPELLIER, INSTITUT CURIEInventors: Jamal Tazi, Didier Scherrer, Aude Garcel, Noëlie Campos, Romain Najman, Florence Mahuteau-Betzer
-
Patent number: 11435334Abstract: To give reliability to statistical data on samples as discrete materials, a required sample-number determination device includes: a required sample number for each class acquisition unit configured to acquire a required sample number for each class Ni by the following Equation (1) based on a proportion P{circumflex over (?)}i, that is a ratio of the number of samples in each class to the number of the samples in the population; a temporary required sample number acquisition unit configured to acquire a temporary required sample number Nr, which may be a maximum value among the required sample numbers for each class Ni; and a required sample number determination unit configured to determine the temporary required sample number Nr as a true required sample number when the sample number reaches the temporary required sample number Nr or more, [ Mathematical ? ? 1 ] ? N i = ( K P ? i ) 2 ? P ^ i ? ( 1 - P ^ i ) ( 1 ) in Equation (1), ?i denotes aType: GrantFiled: June 25, 2018Date of Patent: September 6, 2022Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Takao Ueda, Tatsuya Oki
-
Patent number: 11434267Abstract: The present invention provides a family of non-naturally occurring polypeptides having cholesterol efflux activity that parallels that of full-length apolipoproteins (e.g., Apo AI and Apo E), and having high selectivity for ABCA1 that parallels that of full-length apolipoproteins. Further, the peptides of the invention have little or no toxicity when administered at therapeutic and higher doses. The invention also provides compositions comprising such polypeptides, methods of identifying, screening and synthesizing such polypeptides, and methods of treating, preventing or diagnosing diseases and disorders associated with dyslipidemia, hypercholesterolemia, or inflammation; or diseases involving abnormal glucose metabolism, e.g., diabetes, metabolic syndrome; or Alzheimer's Disease or frontotemporal dementia.Type: GrantFiled: September 10, 2020Date of Patent: September 6, 2022Assignee: The Regents of the University of CaliforniaInventors: John K. Bielicki, Jan Johansson, Waleed Danho
-
Patent number: 11434522Abstract: A method of determining the epigenetic chromosome interactions which are relevant to a companion diagnostic.Type: GrantFiled: June 24, 2016Date of Patent: September 6, 2022Assignee: Oxford Biodynamics PLCInventors: Ewan Hunter, Aroul Ramadass, Alexandre Akoulitchev
-
Patent number: 11430545Abstract: Various methods, systems, computer readable media, and graphical user interfaces (GUIs) are presented and described that enable a subject, doctor, or user to characterize or classify various types of cancer precisely. Additionally, described herein are methods, systems, computer readable media, and GUIs that enable more effective specification of treatment and improved outcomes for patients with identified types of cancer. Some embodiments of the methods, systems, computer readable media, and GUIs described herein comprise obtaining RNA expression data and/or whole exome sequencing (WES) data for a biological sample; determining a molecular-functional (MF) profile for the subject at least in part by determining first and second visual characteristics for first and second GUI elements using the data; generating a personalized GUI personalized to the subject using the first and second visual characteristics; and presenting the generated personalized GUI to a user.Type: GrantFiled: June 12, 2018Date of Patent: August 30, 2022Assignee: BostonGene CorporationInventors: Alexander Bagaev, Feliks Frenkel, Nikita Kotlov, Ravshan Ataullakhanov, Olga Isaeva
-
Patent number: 11426423Abstract: The methods and assays described herein relate to detection, diagnosis, and treatment of lung cancer, e.g., by detecting the level of expression of certain miRNAs described herein and/or by therapeutically increasing the level of those miRNAs.Type: GrantFiled: March 24, 2020Date of Patent: August 30, 2022Assignee: Trustees of Boston UniversityInventors: Ana Brandusa Pavel, Joshua David Campbell, Marc Elliott Lenburg, Avrum Elliot Spira
-
Patent number: 11427859Abstract: Provided for herein is a method for detecting the presence of a nucleic acid target molecule in a biological sample. In certain aspects, the method comprises contacting a test sample that comprises (i) a biological sample comprising a nucleic acid target molecule and (ii) an osmylated single-stranded oligonucleotide probe comprising at least one pyrimidine residue covalently bonded to a substituted or unsubstituted Osmium tetroxide (OsO4)-2,2?-bypyridine group (OsBp group).Type: GrantFiled: September 3, 2021Date of Patent: August 30, 2022Inventor: Anastassia Kanavarioti
-
Patent number: 11421212Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).Type: GrantFiled: August 4, 2017Date of Patent: August 23, 2022Assignee: CARGILL, INCORPORATEDInventors: Christopher K. Miller, Gregory Michael Poynter, Amit Vas
-
Patent number: 11421265Abstract: A method of analyzing a tumor sample comprising: (a) acquiring a library comprising a plurality of tumor members from a tumor sample; (b) contacting the library with a bait set to provide selected members; (c) acquiring a read for a subgenomic interval from a tumor member from said library; (d) aligning said read; and (e) assigning a nucleotide value (e.g., calling a mutation) from said read for the preselected nucleotide position, thereby analyzing said tumor sample.Type: GrantFiled: January 11, 2019Date of Patent: August 23, 2022Assignee: FOUNDATION MEDICINE, INC.Inventors: Doron Lipson, Geoffrey Alan Otto, Alexander Nevin Parker, Philip James Stephens, Sean R. Downing, Mima Jarosz, Mikhail G. Shapiro, Roman Yelensky
-
Patent number: 11421284Abstract: The present invention features methods, devices, and kits for detecting expression in a patient having cancer or determining responsive of a patient having cancer to a treatment, such as irofulven. The invention further includes methods of treating a patient having cancer by administering, e.g., irofulven.Type: GrantFiled: July 13, 2020Date of Patent: August 23, 2022Assignee: Allarity Therapeutics Europe ApSInventor: Steen Knudsen
-
Patent number: 11401560Abstract: Disclosed are a set of genes for bladder cancer detection and their use. The set of genes includes the following 32 genes: CA9 gene, CDK1 gene, CTSE gene, DMBT1 gene, ERBB2 gene, HOXA13 gene, IGF2 gene, CXCR2 gene, MAGEA3 gene, MDK gene, MMP1 gene, MMP12 gene, RBP2 gene, CCL18 gene, SNAI2 gene, VEGFA gene, MFAP5 gene, SGK2 gene, WFDC2 gene, POSTN gene, NPFFR2 gene, ANXA10 gene, CTAG2 gene, ZDHHC2 gene, KRT20 gene, PPP1R14D gene, FGD3 gene, AHNAK2 gene, SEMA3D gene, ZNF707 gene, LOC100652931 gene, and LINC00565 gene. After clinical validation, the kit provided by the present invention is used to detect bladder cancer with a high accuracy rate and objective interpretation of results. Meanwhile, as a non-invasive detection, the compliance of patients is greatly improved comparing with the existing cystoscopy, which has an important clinical significance for the early detection and postoperative monitoring of bladder cancer.Type: GrantFiled: January 17, 2019Date of Patent: August 2, 2022Assignee: HANGZHOU CANHELP GENOMICS TECHNOLOGY CO. LTD.Inventors: Qinghua Xu, Dingwei Ye, Qifeng Wang, Chengshu Chen, Jinying Chen, Yifeng Sun, Wanli Ren, Hongying Wang, Yiwang Wu, Tao Huo
-
Patent number: 11402373Abstract: The present invention relates to detection molecules comprising at least one binding molecule, at least one linker and at least one label, and detection methods making use of same. The invention provides a high-throughput method for detection, isolation and/or identification of specific entities or cells.Type: GrantFiled: February 10, 2022Date of Patent: August 2, 2022Assignees: Immudex ApS, Herlev HospitalInventors: Henrik Pedersen, Søren Jakobsen, Sine Reker Hadrup, Amalie Kai Bentzen, Kristoffer Haurum Johansen
-
Patent number: 11398296Abstract: Methods, sample vessels, and instruments are provided for melting curve analysis. A sample is amplified by thermal cycling the sample well between at least an annealing temperature and a denaturation temperature through n cycles, where each cycle includes an in-cycle denaturation step. Fluorescent data is collected during the in-cycle denaturation step of n cycles where n is at least two. Then a composite melting curve is generated using the fluorescent data collected during the denaturation step of each of the n cycles, and the sample is called using the composite melting curve, where the call is a positive or a negative call.Type: GrantFiled: June 21, 2018Date of Patent: July 26, 2022Assignee: BIOFIRE DIAGNOSTICS, LLCInventors: Benjamin William Galvin, Thomas Charles Robbins, Charles Benjamin Cox
-
Patent number: 11397182Abstract: The present invention is directed to a method for identifying an individual who is at risk for developing metastatic liver disease that involves measuring, in a sample isolated from the individual, exosomal levels of one or more markers of metastatic liver disease. Kits for carrying out this method are also disclosed. The present invention also relates to a method of preventing metastatic liver disease in an individual who are at risk for developing the disease that involves administering one or more inhibitors of liver pre-metastatic niche formation.Type: GrantFiled: October 7, 2015Date of Patent: July 26, 2022Assignee: CORNELL UNIVERSITYInventors: David C. Lyden, Bruno Costa Da Silva
-
Patent number: 11397882Abstract: Disclosed herein are methods and systems for determining the numbers of targets. In some embodiments, the method comprise: stochastically barcoding targets using stochastic barcodes; obtaining sequencing data; for one or more of the targets: counting the number of molecular labels with distinct sequences associated with the target in the sequencing data; identifying clusters of molecular labels of the target using directional adjacency; collapsing the sequencing data using the clusters of molecular labels of the target identified; and estimating the number of the target.Type: GrantFiled: May 25, 2017Date of Patent: July 26, 2022Assignee: Becton, Dickinson and CompanyInventors: Jue Fan, Jennifer Tsai, Eleen Shum, Lisha Deng, Glenn K. Fu
-
Patent number: 11377690Abstract: The subject of the invention is a method for making an in vitro prognosis of severity for a patient in septic shock, including the following steps: (i) the level of expression of the expression product of at least one gene chosen from the lilrb2 and lilrb1 genes is measured in vitro on the basis of a biological sample taken from the patient, (ii) the level of expression of the expression product of the at least one gene is compared with a control level of expression, of the expression product of the same gene, with a good prognosis of severity, in which a level of expression of the expression product of the at least one gene below the control level of expression indicates a poor prognosis of severity for the patient, and also a kit for implementing the method.Type: GrantFiled: January 10, 2014Date of Patent: July 5, 2022Assignees: BIOMERIEUX, HOSPICES CIVILS DE LYONInventors: Marie-Angelique Cazalis, Maud Tournoud, Fabienne Venet, Alain Lepape, Guillaume Monneret
-
Patent number: 11371096Abstract: The invention relates to methods and kits for diagnosing and/or treating appendicitis in a subject, comprising performing one or more assays configured to detect one or more biomarkers on a body fluid sample obtained from the subject to provide one or more assay result(s) and correlating the assay result(s) to the occurrence or nonoccurrence of appendicitis in the subject or likelihood of the future outcome to the subject.Type: GrantFiled: October 22, 2015Date of Patent: June 28, 2022Assignee: The George Washington University A Congressionally Chartered Not-For-Profit CorporationInventors: Lakhmir S. Chawla, Timothy A. McCaffrey