Enzyme Component Of Specific Activity Or Source (e.g., Protease, Of Bacterial Origin, Etc.) Patents (Class 510/300)
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Patent number: 7605115Abstract: New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.Type: GrantFiled: April 17, 2007Date of Patent: October 20, 2009Assignees: Novozymas Als, Maxygen Inc.Inventors: Jon E. Ness, Mark Welch, Lorraine J. Giver, Joel R. Cherry, Torben V. Borchert, Jeremy Minshull
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Patent number: 7601529Abstract: The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.Type: GrantFiled: May 14, 2003Date of Patent: October 13, 2009Assignee: Novozymes A/SInventors: Sanne Schroder Glad, Carsten Andersen, Torben Vedel Borchert, Katja Salomon Johansen, Henrik Frisner, Thomas Thisted, Mads Eskelund Bjornvad
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Publication number: 20090233837Abstract: Laundry detergent compositions that provide soil release benefits to all fabric comprising an organophosphorus soil release agents and optional non-cotton secondary soil release agents. The present invention further relates to a method for providing soil release benefits to cotton fabric by contacting cotton articles with a water soluble and/or dispersible organophosphorus material. The contacting can be during washing or by pretreating by applying the composition directly to stains or by presoaking the clothing in the composition prior to washing. The present invention further relates to providing soil release benefits to all fabric in the laundry wash load in the presence of a bleaching agent.Type: ApplicationFiled: May 25, 2009Publication date: September 17, 2009Applicant: Rhodia Inc.Inventors: Tobias Johannes FUTTERER, Lawrence Alan HOUGH, Robert Lee REIERSON
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Publication number: 20090192066Abstract: Detergent compositions containing high efficiency lipase enzymes and specific detergent formulations comprising less than 10 wt % zeolite and phosphate builder are described. Preferred formulations comprise surfactants selected from alkyl benzene sulphonates in combination with alky ethoxylated sulfates or MES or non-ionic surfactants.Type: ApplicationFiled: March 18, 2009Publication date: July 30, 2009Inventors: Neil Joseph Lant, Steven George Patterson
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Publication number: 20090181874Abstract: This invention relates to compositions comprising certain cellobiose dehydrogenase enzymes and processes for making and using such compositions, including the use of such compositions to clean and/or treat a situs.Type: ApplicationFiled: January 6, 2009Publication date: July 16, 2009Inventors: Philip Frank Souter, Eva Maria Perez-Prat Vinuesa, Enrique Herrero Acero, Sina Pricelius, Georg Maximillian Guebitz
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Publication number: 20090176680Abstract: The present invention relates to the use of a cellulase to impart soil release benefits to cotton during a subsequent laundering process.Type: ApplicationFiled: December 22, 2008Publication date: July 9, 2009Inventors: Steven George Patterson, Neil Joseph Lant
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Patent number: 7553807Abstract: Washing is carried out using a detergent composition having main detergency obtained through an alkaline inorganic salt and further including at least an anti-soil redeposition agent. There is provided a clothes washing method, and a detergent composition for the same, that uses a detergent having detergency equivalent to or greater than that of synthetic detergents containing a surface active agent as the main detergency ingredient and also has excellent anti-soil redeposition efficiency wherein main detergency is obtained by an alkaline inorganic salt.Type: GrantFiled: July 3, 2008Date of Patent: June 30, 2009Assignee: Miz Co., Ltd.Inventors: Kazuyoshi Arai, Tomoki Seo
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Patent number: 7550420Abstract: A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.Type: GrantFiled: October 27, 2006Date of Patent: June 23, 2009Assignee: E. I. DuPont De Nemours and CompanyInventors: Robert DiCosimo, Mark Scott Payne, Eugenia Costa Hann, Vincent Brian Croud, John Edward Gavagan, Lorraine Winona Wagner
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Patent number: 7537922Abstract: The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.Type: GrantFiled: February 27, 2007Date of Patent: May 26, 2009Assignee: Novozymes A/SInventors: Mads Norregaard-Madsen, Peter Rahbek Østergaard, Claus Bo Voge Christensen, Søren Flensted Lassen
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Patent number: 7538079Abstract: The present invention relates to a process for the manufacture of a spray dried powder comprising: (a) forming a warm, stirred aqueous slurry comprising: inorganic salts, at least one binding agent and 0.001 to 20% by weight of capsules based on the weight of spray dried powder, said capsules containing benefit agents including at least perfume, and (b) spray drying the resultant slurry to form a spray dried powder, said capsules being such that: 1) more than 40% of the benefit agents remain encapsulated 60 minutes after dispersion thereof at 70° C. in the slurry as defined in the “slurry survival test” in a sealed vessel without agitation; and 2) more than 30% of the benefit agents added for 15 minutes to an ambient slurry as defined in the “spray dry test” survive spray drying through a laboratory scale spray drier.Type: GrantFiled: September 22, 2006Date of Patent: May 26, 2009Assignee: Takasago International CorporationInventors: Jonathan Warr, Stuart Fraser, Emmanuel Aussant
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Patent number: 7517846Abstract: The invention is a solid chemical concentrate system of at least two cooperative shapes. The first shape is an inwardly curved bar having an inner opening. The second shape is an insert which is capable of interlocking with the bar by insertion into the bar inner opening. The solid chemical concentrate provides chemical systems having active constituents which may be the same, different but compatible or functionally and chemically incompatible combined within one matrix to provide at least one substantially continuous surface. The system may also comprise an aqueous soluble or dispersible polymeric film cover.Type: GrantFiled: October 20, 2005Date of Patent: April 14, 2009Assignee: Ecolab Inc.Inventors: Elizabeth J. Gladfelter, Tina O. Outlaw, James L. Copeland, Rhonda K. Schulz, Daniel K. Boche, Jeff W. Peterson
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Patent number: 7517847Abstract: Bleaching product in the form of a coated substrate comprising a water-soluble film and a coating comprising a bleaching agent.Type: GrantFiled: May 11, 2006Date of Patent: April 14, 2009Assignee: The Procter & Gamble CompanyInventors: Vincenzo Catalfamo, Frank William Denome, Dennis Allen Beckholt, Yousef Georges Aouad, James Michael Archbold
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Patent number: 7510859Abstract: The invention relates to novel perhydrolases, derived from the protease Carlsberg by amino acid exchange in positions 11, 15, 21, 38, 50, 54, 58, 77, 83, 89, 93, 96, 107, 117, 120, 134, 135, 136, 140, 147, 150, 154, 155, 160, 161, 171, 179, 180, 181, 194, 205, 208, 213, 216, 217, 238, 239, 251, 253, 257, and/or 261. The invention further relates to methods for production of said novel perhydrolases, products comprising said novel perhydrolases, particularly bodycare, haircare, shampoo, hair-dyeing, hair-bleaching, oral-care, dental-dare, dental-prosthesis-care, cosmetic, therapeutic (textile) washing, cleaning, rinsing, handwash, washing-up and dish-washing products, and corresponding applications of said novel perhydrolases.Type: GrantFiled: June 17, 2005Date of Patent: March 31, 2009Assignee: Henkel Kommanditgesellschaft Auf AktienInventors: Susanne Wieland, Laura Polanyi-Bald, Inken Prueser, Regina Stehr, Karl-Heinz Maurer
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Patent number: 7507569Abstract: Novel subtilases having an improved wash performance on egg stains are disclosed. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.Type: GrantFiled: May 6, 2004Date of Patent: March 24, 2009Assignees: Novozymes A/S, Maxygen Inc.Inventors: Stefan Minning, Jurgen Carsten Franz Knotzel, Niels Henrik Sorensen, Jon E. Ness, Mark D. Welch, Lorraine J. Giver, Joel Cherry, Torben Vedel Borchert, Jeremy Minshull
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Patent number: 7476528Abstract: The present invention relates to novel protein variants that exhibit reduced allergenicity when compared to the parental proteins. Also included are DNA molecules that encode the novel variants, host cells comprising the DNA and methods of making proteins less allergenic.Type: GrantFiled: May 12, 2005Date of Patent: January 13, 2009Assignees: Genencor International, Inc., The Procter & Gamble CompanyInventors: David A. Estell, Grant C. Ganshaw, Fiona A. Harding, Edmund A. Larenas, Ayrookaran J. Poulose, Elizabeth E. Sikorski, Elliott P. Russell
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Patent number: 7473544Abstract: The present invention provides an alkaline protease exhibiting high detergency and productivity, which is obtained by further enhancing the detergency of an alkaline protease effective against complex dirt and enhancing the specific activity.Type: GrantFiled: September 27, 2005Date of Patent: January 6, 2009Assignee: Kao CorporationInventors: Mitsuyoshi Okuda, Tsuyoshi Sato, Yasushi Takimura, Nobuyuki Sumitomo, Tohru Kobayashi
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Patent number: 7445922Abstract: This invention relates to a protein that is a Zygomycetes-derived endoglucanase lacking the cellulose-binding domain and exhibits endoglucanase activity, and a method for using the same. This invention can enhance effects of an endoglucanase enzyme on fabric treatment such as reduction of fuzzing, improvement in feel and appearance, color clarification, partial color change, and softening of cellulose-containing fabrics and on performance improvement in the deinking of waste paper and drainage of paper pulp.Type: GrantFiled: November 21, 2001Date of Patent: November 4, 2008Assignee: Meiji Seika Kaisha, Ltd.Inventors: Akitaka Nakane, Yuko Baba, Jinichiro Koga, Hidetoshi Kubota
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Patent number: 7439217Abstract: The invention is directed to a liquid laundry detergent composition comprising at least one detergent ingredient selected from the group consisting of anionic surfactant, zwitterionic surfactant, amphoteric surfactant, and mixtures thereof; a coacervate phase forming cationic polymer; and one or more fabric care ingredients selected from the group consisting of one or more cationic silicone polymers comprising one or more polysiloxane units and one or more nitrogen moieties; one or more amino silicone polymers; one or more nitrogen-free silicone polymers; and mixtures thereof; and a liquid carrier for providing cleaning and fabric care benefits. A process for preparing such compositions, a method for treating substrates, a method for providing certain fabric care benefits and the use of such compositions are also described.Type: GrantFiled: June 15, 2007Date of Patent: October 21, 2008Assignee: The Procter & Gamble CompanyInventors: Jean-Pol Boutique, Patrick Firmin August Delplancke, Roland Wagner, Matthew David Butts, Sarah Elizabeth Genovese, Stefano Scialla
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Patent number: 7429476Abstract: The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.Type: GrantFiled: December 20, 2005Date of Patent: September 30, 2008Assignee: Genencor International, Inc.Inventors: Kathleen A. Clarkson, Nigel Dunn-Coleman, Suzanne E. Lantz, Craig E. Pilgrim, Piet van Solingen, Michael Ward
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Patent number: 7425528Abstract: The present invention relates to granules comprising a core matrix and one or more coatings, wherein the core matrix comprises: An active compound, a synthetic polymer, where the amount of polymer added is 0.1 to 10% by weight of the matrix and one or more antioxidants and/or reducing agents, where the amount of antioxidant and/or reducing agent is 0.2 to 5% by weight of the matrix.Type: GrantFiled: June 30, 2003Date of Patent: September 16, 2008Assignee: Novozymes A/SInventors: Ole Simonsen, Erik Kjaer Markussen, Hanne Rojel, Svend Kaasgaard, Thomas Honger Callisen, Christian Isak Jorgensen
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Patent number: 7424891Abstract: Combination products comprising packaging and two liquid cleansers or detergents A and B separated from each other in the packaging and having the following composition: A: 10% to 75% by weight of detergent builder(s), 0.1% to 10% by weight of enzyme(s), 24.9% to 89.9% by weight of water; and B: 10% to 74.9% by weight of detergent builder(s), 25% to 89.9% by weight of water, 0.1% to 15% by weight of bleach, characterized in that the liquid detergent A has a pH value (at 20° C.) between 6 and 9. The combination products of the invention demonstrate improved cleaning power over conventional solid or liquid dishwashing detergents.Type: GrantFiled: March 16, 2006Date of Patent: September 16, 2008Assignee: Henkel AG & Co. KGaAInventors: Pavel Gentschev, Christian Nitsch, Ulrich Pegelow, Arnd Kessler, Maren Jekel, Johannes Zipfel
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Publication number: 20080214425Abstract: Detergent compositions containing high efficiency lipase enzymes and specific detergent formulations comprising a high reserve alkalinity, greater than 7.5, and less than 15 wt % zeolite and phosphate builder are described. Preferred formulations comprise surfactants selected from alkyl benzene sulphonates in combination with alkyl ethoxylated sulfates or MES or non-ionic surfactants.Type: ApplicationFiled: February 11, 2008Publication date: September 4, 2008Inventors: Neil Joseph Lant, Steven George Patterson
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Patent number: 7407924Abstract: Washing is carried out using a detergent composition having main detergency obtained through an alkaline inorganic salt and further including at least an anti-soil redeposition agent. There is provided a clothes washing method, and a detergent composition for the same, that uses a detergent having detergency equivalent to or greater than that of synthetic detergents containing a surface active agent as the main detergency ingredient and also has excellent anti-soil redeposition efficiency wherein main detergency is obtained by an alkaline inorganic salt.Type: GrantFiled: December 5, 2001Date of Patent: August 5, 2008Assignee: MIZ Co., Ltd.Inventors: Kazuyoshi Arai, Tomoki Seo
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Patent number: 7368273Abstract: An alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 17-0, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 2 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (e): histidine, aspartic acid, phenylalanine, lysine, asparagine, seine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (K): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): seine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine.Type: GrantFiled: March 12, 2003Date of Patent: May 6, 2008Assignee: Kao CorporationInventors: Mitsuyoshi Okuda, Tsuyoshi Sato, Kazuhiro Saito, Nobuyuki Sumitomo, Yoshifumi Izawa, Katsuhisa Saeki, Tohru Kobayashi, Masafumi Nomura
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Patent number: 7335630Abstract: The invention is directed to aqueous liquid laundry detergent compositions for cleaning and imparting fabric care benefits to fabrics laundered therewith. Such compositions comprise (A) at least one detersive surfactant; (B) droplets of a silicone blend comprising a nitrogen-containing amino or ammonium functionalized polysiloxane and a nitrogen-free non-functionalized polysiloxane; and (C) at least one additional non-silicone laundry adjunct selected from detersive enzymes, dye transfer inhibiting agents, optical brighteners, suds suppressors and combinations thereof. The functionalized polysiloxane component of the silicone blend has a relatively low, i.e., less than 30 mol %, content of reactive/curable groups, a nitrogen content which ranges from 0.05% to 0.30% by weight and a viscosity which ranges from 0.00002 m2/s to 0.2 m2/s. The nitrogen-free non-functionalized polysiloxane material ranges in viscosity from 0.01 m2/sec to 2.0 m2/sec.Type: GrantFiled: April 15, 2005Date of Patent: February 26, 2008Assignee: The Procter & Gamble CompanyInventors: Patrick Firmin August Delplancke, Jean-Pol Boutique, Stefano Scialla, Connie Lynn Sheets, James Charles Theophile Roger Burckett St Laurent, Hugo Robert Germain Denutte
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Patent number: 7332320Abstract: The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins.Type: GrantFiled: December 20, 2002Date of Patent: February 19, 2008Assignee: Genencor International, Inc.Inventors: David A Estell, Fiona A. Harding
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Patent number: 7326676Abstract: The invention is directed to aqueous liquid laundry detergent compositions for cleaning and imparting fabric care benefits to fabrics laundered therewith. Such compositions comprise (A) at least one detersive surfactant; (B) droplets of a silicone blend comprising a nitrogen-containing amino or ammonium functionalized polysiloxane and a nitrogen-free non-functionalized polysiloxane; and (C) at least one additional non-silicone laundry adjunct selected from detersive enzymes, dye transfer inhibiting agents, optical brighteners, suds suppressors and combinations thereof. The functionalized polysiloxane component of the silicone blend has a relatively low, i.e., less than 30 mol %, content of reactive/curable groups, a nitrogen content which ranges from 0.05% to 0.50% by weight and a viscosity which ranges from 0.00002 m2/s to 0.2 m2/s. The nitrogen-free, non-functionalized polysiloxane material ranges in viscosity from 0.01 m2/sec to 2.0 m2/sec.Type: GrantFiled: July 2, 2004Date of Patent: February 5, 2008Assignee: The Procter & Gamble CompanyInventors: Patrick Firmin August Delplancke, Jean-Pol Boutique, Stefano Scialla, Gregory Leo Jervier, Connie Lynn Sheets
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Patent number: 7326677Abstract: The invention is directed to liquid laundry detergent compositions for treating non-keratinous substrates under domestic wash conditions, such composition comprise (A) at least one surfactant selected from the group consisting of anionic surfactants, nonionic surfactants, zwitterionic surfactants, amphoteric surfactants, and combinations thereof; (B) a silicone blend comprising a non-functionalized silicone and a functionalized silicone; and (C) at least one additional non-silicone laundry adjunct selected from the group consisting of detergent builders, detersive enzymes, dye transfer inhibiting agents, and combinations thereof. The claimed compositions are further essentially free of any coacervate phase-forming polymer and essentially free of any cationic deposition aid.Type: GrantFiled: July 2, 2004Date of Patent: February 5, 2008Assignee: The Procter & Gamble CompanyInventors: Patrick Firmin August Delplancke, Jean-Pol Boutique, Stefano Scialla, Gregory Leo Jervier, Connie Lynn Sheets
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Patent number: 7320887Abstract: The invention relates to novel alkaline protease variants. These variants have, when enumerating the alkaline protease from Bacillus lentus, variations in amino acid position 61, positions 199 and/or 211 and, optionally, at least one modification that contributes to the stabilization of the molecule, said modification preferably being point mutations in positions 3 and/or 4. Particularly preferred are variants S3T/V41/G61A/V199] and S3T/V41/G61A/V1991/L211D of B. lentus alkaline protease. The invention also relates to the possible use of these enzymes in diverse technical processes and, in particular, to detergents and cleansers containing these novel alkaline protease variants.Type: GrantFiled: April 30, 2004Date of Patent: January 22, 2008Assignee: Henkel Kommanditgesellschaft auf AktienInventors: Beatrix Kottwitz, Karl-Heinz Maurer, Roland Breves
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Patent number: 7306937Abstract: Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or re-combinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.Type: GrantFiled: January 16, 2003Date of Patent: December 11, 2007Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, David A Estell, James T Kellis, Jr., Richard R. Bott
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Patent number: 7294499Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: GrantFiled: February 24, 2004Date of Patent: November 13, 2007Assignee: Novozymes A/SInventors: Allan Svendsen, Henriette Draborg
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Patent number: 7288401Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.Type: GrantFiled: April 12, 2005Date of Patent: October 30, 2007Assignee: Novozymes A/SInventors: Peter Kamp Hansen, Peter Bauditz, Frank Mikkelsen, Kim Vilbour Andersen
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Patent number: 7273837Abstract: The invention is directed to a liquid laundry detergent composition comprising at least one detergent ingredient selected from the group consisting of anionic surfactant, zwitterionic surfactant, amphoteric surfactant, and mixtures thereof; a coacervate phase forming cationic polymer; and one or more fabric care ingredients selected from the group consisting of one or more cationic silicone polymers comprising one or more polysiloxane units and one or more nitrogen moieties; one or more amino silicone polymers; one or more nitrogen-free silicone polymers; and mixtures thereof; and a liquid carrier for providing cleaning and fabric care benefits. A process for preparing such compositions, a method for treating substrates, a method for providing certain fabric care benefits and the use of such compositions are also described.Type: GrantFiled: November 4, 2003Date of Patent: September 25, 2007Assignee: The Procter & Gamble CompanyInventors: Jean-Pol Boutique, Patrick Firmin August Delplancke, Roland Wagner, Matthew David Butts, Sarah Elizabeth Genovese, Stefano Scialla
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Patent number: 7256032Abstract: The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.Type: GrantFiled: December 22, 2005Date of Patent: August 14, 2007Assignee: AB Enzymes OyInventors: Leena Valtakari, Marika Alapuranen, Satu Hooman, Matti Siika-Aho, Jarno Kallio, Liisa Viikari, Pentti Ojapalo, Jari Vehmaanperä
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Patent number: 7250281Abstract: Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc. The substituted amino acid residues correspond to positions +76 in combination with one or more of the following residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 in Bacillus amyloliquefaciens subtilisin.Type: GrantFiled: December 18, 2002Date of Patent: July 31, 2007Assignee: Genencor International, Inc.Inventors: Thomas P. Graycar, Richard R. Bott, Lori J. Wilson
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Patent number: 7220566Abstract: New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.Type: GrantFiled: December 15, 2003Date of Patent: May 22, 2007Assignees: Novozymes A/s, Maxygen, Inc.Inventors: Jon E. Ness, Mark Welch, Lorraine J. Giver, Joel R. Cherry, Torben V. Borchert, Jeremy Minshull
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Patent number: 7220716Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: GrantFiled: August 25, 2004Date of Patent: May 22, 2007Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7217554Abstract: The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.Type: GrantFiled: August 11, 2003Date of Patent: May 15, 2007Assignee: Novozymes A/SInventors: Mads Nørregaard-Madsen, Peter Rahbek Østergaard, Claus Bo Vøge Christensen, Søren Flensted Lassen
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Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
Patent number: 7202074Abstract: The present invention provides nucleic acid and corresponding amino acid sequences of two isoforms of cold adapted trypsin-like activity protein, insolated from antarctic marine origin, preferably from antarctic krill (Euphausia superba) that can be used in a variety of industrial contexts and commercial purposes including laundry detergents, food processing, drugs and skin care products. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the cold adapted trypsin-like protein.Type: GrantFiled: July 22, 2004Date of Patent: April 10, 2007Assignee: University of ChileInventors: Juan A. Asenjo, Barbara A. Andrews, Fernando Reyes, Mauricio Salamanca, Luis Burzio -
Patent number: 7192757Abstract: The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42–43, 51–56, 155–161, 187–190, 216–217, 217–218 or 218–219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.Type: GrantFiled: July 2, 2004Date of Patent: March 20, 2007Assignee: Novozymes A/SInventors: Mads Nørregard-Madsen, Line Bloch Larsen, Peter Kamp Hansen
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Patent number: 7189553Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.Type: GrantFiled: December 6, 2002Date of Patent: March 13, 2007Assignee: Novozymes A/SInventors: Dorrit Aaslyng, Sven Branner, Sven Hastrup, Leif Norskov-Lauritsen, Ole Hvilsted Olsen, Merete Simonsen, Eric Castelijn, Maarten Robert Egmond, Johan Haverkamp, John David Marugg, Arnoldus Theodorus Anthonius Mooren
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Patent number: 7179630Abstract: The present invention relates to thermostable proteases having an amino acid sequence which homologous to the amino acid sequence of proteases derived from Nocardiopsis, and the production thereof by wild-type and recombinant host cells including transgenic plants and non-human transgenic animals. The proteases are effective in animal feed, in particular fish feed, and detergents. The proteases are capable of degrading the soybean Bowman-Birk inhibitor, and other antinutritional factors such as soybean agglutinin and the Kunitz trypsin inhibitor, as well as the isolated soy storage proteins glycinin and beta-conglycinin. Characteristic structural features of relevance for the thermostability of these proteases of peptidase family S2A or S1E are disclosed.Type: GrantFiled: June 21, 2004Date of Patent: February 20, 2007Assignee: Novozymes A/SInventors: Soeren Flensted Lassen, Carsten Sjoeholm, Peter Rahbek Oestergaard, Carsten Andersen, Morten Fischer
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Patent number: 7138263Abstract: There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.Type: GrantFiled: May 22, 2001Date of Patent: November 21, 2006Assignee: Meiji Seika Kabushiki KaishaInventors: Koichiro Murashima, Naomi Sumida, Akitaka Nakane, Koji Yanai, Tomoko Nishimura, Jinichiro Koga, Takeshi Murakami, Toshiaki Kono
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Patent number: 7129076Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease.Type: GrantFiled: April 25, 2003Date of Patent: October 31, 2006Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Patent number: 7109016Abstract: The present invention relates to subtilases having a reduced tendency towards inhibition by substances present in eggs, such as the trypsin inhibitor type IV-0. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: GrantFiled: August 16, 2001Date of Patent: September 19, 2006Assignee: Novozymes A/SInventors: Helle Outtrup, Poul Erik Pedersen, Marianne Vind Sørensen
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Patent number: 7098017Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.Type: GrantFiled: September 6, 2001Date of Patent: August 29, 2006Assignee: Novozymes A/SInventors: Claus von der Osten, Torben Halkier, Carsten Andersen, Peter Bauditz, Peter Kamp Hansen
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Patent number: 7078372Abstract: The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: August 24, 2004Date of Patent: July 18, 2006Assignee: Genencor International, Inc.Inventor: David A. Estell
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Patent number: 7056879Abstract: Laundry product compositions containing a stable mixture of at least one water insoluble fabric care benefit agent and at least one delivery enhancing agent that is preferably a silicone derivative. Detergent laundry product compositions containing from about 1% to about 80%, by weight of the composition, of a detersive surfactant that is an anionic surfactant, cationic surfactant, nonionic surfactant, amphoteric surfactant, zwitterionic surfactant, or a mixture thereof; from about 0.1% to about 10%, by weight of the composition, of a water insoluble fabric care benefit agent; from about 0.01% to about 5%, by weight of the composition, of a delivery enhancing agent; and wherein the ratio of the delivery enhancing agent to the fabric care benefit agent is from about 1:50 to about 1:1.Type: GrantFiled: September 9, 2003Date of Patent: June 6, 2006Assignee: The Procter & Gamble CompanyInventors: Jiping Wang, Rajan Keshav Panandiker, Paul Francis Kindel, Mary Ruth Leyendecker
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Patent number: 7056880Abstract: Laundry product compositions containing a stable mixture of at least one water insoluble fabric care benefit agent and at least one delivery enhancing agent that is preferably a dispersible polyolefin, polymer latex, or mixture thereof. Detergent laundry product compositions containing from about 1% to about 80%, by weight of the composition, of a detersive surfactant that is an anionic surfactant, cationic surfactant, nonionic surfactant, amphoteric surfactant, zwitterionic surfactant, or a mixture thereof; from about 0.1% to about 10%, by weight of the composition, of a water insoluble fabric care benefit agent; from about 0.01% to about 5%, by weight of the composition, of a delivery enhancing agent; and wherein the ratio of the delivery enhancing agent to the fabric care benefit agent is from about 1:50 to about 1:1.Type: GrantFiled: September 9, 2003Date of Patent: June 6, 2006Assignee: The Procter & Gamble CompanyInventors: Jiping Wang, Rajan Keshav Panandiker, Paul Francis Kindel, Mary Ruth Leyendecker
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Patent number: 7033817Abstract: The present invention relates to the identification of a novel metalloproteases in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: June 21, 2002Date of Patent: April 25, 2006Assignee: Genencor International, Inc.Inventor: David A. Estell