Abstract: The present invention relates to cleaning compositions comprising protease enzyme which is a carbonyl hydrolase variant having an amino acid sequence not found in nature, which is derived by replacement of a plurality of amino acid residues of a precursor carbonyl hydrolase with different amino acids, wherein said plurality of amino acid residues replaced in the precursor enzyme correspond to position +76 in combination with one or more of the following residues: +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, and/or +274, where the numbered positions corresponds to naturally-occurring subtilisin from Bacillus amyloliquefaciens or to equivalent amino acid residues in other carbonyl hydrolases or subtilisins (such as Bacillus lentus subtilisin).

Abstract: A purified novel cellulase composition is provided which may be isolated from a fermentation culture of Trichoderma longibrachiatum and has a molecular weight of about 95-105 kD as approximated on SDS-PAGE (see FIG. 1), a pl of about 5.6-6.8 as estimated on an IEF gel and a pH optimum of about 5.0 on RBB-CMC when measured at 65.degree. C. and pH 4 or lower at temperatures of 40.degree. C. and 50.degree. C.

Abstract: The present invention provides a contact lens cleaning solution which is capable of, simultaneously and in a simplified manner, rendering surfaces of the contact lens hydrophilic and cleaning the contact lens for removal of protein deposits, without suffering from reduction of the cleaning effect of a protease, and also provides a method of cleaning a contact lens which is capable of rendering the surfaces of the contact lens hydrophilic while, at the same time, cleaning the contact lens for removal of the protein deposits, by using the contact lens cleaning solution as described above.A desired contact lens cleaning solution is prepared, which contains a protease in an amount effective for removing protein adhering or clinging to the contact lens and gum arabic as a hydrophilicity rendering component, wherein divalent metal ions in the gum arabic are removed or inactivated.

Abstract: The present invention relates to a liquid composition comprising an enzyme and a phenyl boronic acid derivative enzyme stabilizer of the following formula: ##STR1## wherein R is selected from the group consisting of hydrogen, hydroxy, C.sub.1 -C.sub.6 alkyl, substituted C.sub.1 -C.sub.6 alkyl, C.sub.1 -C.sub.6 alkenyl and substituted C.sub.1 -C.sub.6 alkenyl.

Abstract: A process is presented for producing stable multi-enzyme containing granules. The process involves the steps of mixing a reversible competitive inhibitor with a first enzyme, adding a second incompatible enzyme, adding a carrier, extruding and granulating the resulting mixture and reducing the moisture content if necessary.

Abstract: Automatic dishwashing detergent compositions comprising a nonionic surfactant having the formula:R.sub.1 O[CH.sub.2 CH(CH.sub.3)O].sub.x [CH.sub.2 CH.sub.2 O].sub.y [CH.sub.2 CH(OH)R.sub.2 ]wherein R.sub.1 is a linear or branched, aliphatic hydrocarbon radical having from about 4 to about 18 carbon atoms including mixtures thereof; R.sub.2 is a linear or branched aliphatic hydrocarbon radical having from about 2 to about 26 carbon atoms including mixtures thereof; x is an integer having an average value of from 0.5 to about 1.5; and y is an integer having a value of least about 15; employed in conjunction with a detersive enzyme such as amylase are provided.

Abstract: This invention concerns cleaning compositions like laundry compositions containing a chondroitinase enzyme. More in particular, the invention relates to compact detergent compositions, granular and liquids as well containing said chondroitinase enzyme.

Abstract: Mild detergent compositions which exhibit good cleaning performance comprise detergent surfactants and small amounts of protease. A preferred embodiment additionally contains suds boosters and divalent ions.

Abstract: Stable liquid enzyme compositions containing an ophthalmically acceptable enzyme and methods involving the combined use of these compositions with a polymeric antimicrobial agent are disclosed for the simultaneous cleaning and disinfecting of contact lens. Methods for a daily use regimen are also disclosed.

Abstract: Stable liquid enzyme compositions containing an ophthalmically acceptable enzyme and methods involving the combined use of these compositions with a polymeric antimicrobial agent are disclosed for the simultaneous cleaning and disinfecting of contact lens. Methods for a daily use regimen are also disclosed.

Abstract: A process for simultaneously desizing and bleaching of a sized fabric containing starch or starch derivatives, which process comprises treating the fabric with a bleaching composition and an oxidation stable .alpha.-amylase.

Abstract: The present invention relates to an alkaline lipolytic enzyme derivable from a strain of Botryosphaeria or Guignardia, to a lipolytic enzyme-producing microbial strain, to methods for the production of lipolytic enzyme and to a detergent composition comprising the lipolytic enzyme.

Abstract: Detergent compositions, essentially free of peroxygen or chlorine bleach compounds, containing a surfactant, builder, enzyme and from about 0.001% to about 5% by weight polyethyleneimine (PEI) or salts thereof are disclosed. These compositions provide enhanced removal of organic stains, particularly on polyphenolic stains such as morello juice (cherry juice), blueberry juice, red wine, tea and coffee. Improvement was also demonstrated on certain non-polyphenolic stains such as grass.

Abstract: Glycolic acid or its salts is effective in stabilizing enzymes in liquid laundry detergent compositions.

Abstract: There is provided an enzymatic detergent composition comprising one or more surfactants and an endoglucanase which is not a Family 7 cellulase, which contains no cellulose binding domain and wherein the catalytic domain contains at least two disulphide bridges. Preferably, the endoglucanase is producible from Thermomonospora fusca, or mutants or variants thereof.

Abstract: This invention relates to a laundry detergent composition as well as a method of laundrying fabric or soiled garments in which the composition requires a surfactant, a pectinase enzyme, a specific hemicellulase enzyme or enzymes, a cellulase enzyme, and additional component which includes bleaching agents, builders, an amylase enzyme and/or a protease enzyme.

Abstract: A mutant cellulase obtainable from Thermomonospora spp is provided which differs from a precursor cellulase in that it has been genetically engineered to introduce a substitution, deletion or addition of an amino acid residue to said precursor cellulase which provided improved activity in a detergent. Preferably, the substitution is at a residue corresponding to T140 in Thermomonospora fusca.

Abstract: This invention is a manual dishwashing composition which comprises surfactant, calcium or magnesium ions, amylase enzymes, lipase enzymes, and polycarboyxlic polymer thickeners. An additional agent includes a calcium stabilizing agent such as malic acid or malic acid salt, maleic acid or maleic acid salt.

Abstract: Aqueous liquid detergent compositions are described which comprise a proteolytic enzyme wherein the proteolytic activity is reversibly inhibited by a peptide trifluoromethyl ketone.

Abstract: The invention concerns detergent compositions comprising lipase variant D96L of the native lipase derived from Humicola lanuginosa present at a level of from 50 LU to 8500 LU per liter wash solution.Using these levels of lipase delivers an improved whiteness maintenance on fabrics.

Abstract: A liquid detergent composition comprising a surfactant, an enzyme and a naphthalene boronic acid derivative enzyme stabilizer.

Abstract: The invention relates to a detergent composition which is a liquid detergent composition which comprises a surfactant, a proteolytic enzyme, and an enzyme inhibitor which is a peptide aldehyde. Said peptide aldehyde inhibitor contain 4 or 5 amino acids. Said peptide aldehydes are used to enhance the stability of proteolytic enzymes in liquid detergent environments.

Abstract: Compositions including one or more enzymes and as the compatible chelating surfactant, salts of N-acyl ethylenediaminetriacetic acid ("ED3A"). Salts of N-acyl ED3A do not readily denature various enzymes, and thus are highly compatible with such enzymes, and enhance their detergent effectiveness to an unexpected degree.

Abstract: The invention relates to a cleanser concentrate having the following composition: 0.5-8.0% by weight of at least one C.sub.5 -C.sub.12 -alkyl sulphate salt, 4.0-15.0% by weight of at least one formulation aid, 4.0-10% by weight of at least one alkanolamine, and at least one commercially conventional proteolytic enzyme in an amount of 0.005-0.1 Anson and, if appropriate, conventional acids or bases for pH adjustment, conventional complexing agents and conventional preservatives, remainder, to 100% by weight, water. A process for cleaning surgical instruments has the following steps: applying a 0.5-100% strength aqueous solution of a cleanser concentrate according to one of claims 1-6 which has a pH of 8-9, allowing the solution to act at a temperature of room temperature to 55.degree. C. for a period of 2 s-20 min, rinsing, thermal disinfection, if appropriate.

Abstract: This invention relates to proteases with improved application properties in cleaners and detergents. The improvement's achieved by substituting positively charged or uncharged amino acids in the substrate binding region of the wild type subtilisin protease.

Abstract: There is provided an enzymatic detergent composition comprising a surfactant and an endoglucanase producible from Thermomonospora fusca, preferably E5, or mutants or variants thereof.

Abstract: The invention discloses stabilized enzymes suitable for use in a contact lens cleansing composition. Specific stabilizers for the protease enzyme include sodium lauroyl sarcosinate, lauroyl-L-glutamic triethanolamine, sodium myristyl sarcosinate, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, hydrochloric alkyldiaminoglycine, polyoxyethylene sorbitan monooleate, polyoxyethylene castor oil 60, polyoxyl 40 stearate, and polyoxyethylene lauryl ether. The invention further discloses that the enzyme and surfactant mixture are lyophilized.

Abstract: The invention provides a high water liquid enzyme prewash composition essentially free of hydrotropes, solvents, dispersants and surfactants, other than nonionic surfactants, combining: a hydrolase enzyme stabilized with a first enzyme stabilizer, wherein the first enzyme stabilizer is a soluble alkaline earth salt; a more hydrophilic, first nonionic surfactant having an HLB of greater than about 11; a more hydrophobic, second nonionic surfactant having an HLB of less than or equal to about 11; and at least about 80-99% water; wherein the difference in HLB between the first and the second nonionic surfactants is at least 2; the nonionic surfactants interact with the water to form an opalescent, structured liquid; the first and the second nonionic surfactants are selected from the group consisting of alkoxylated alcohols and alkoxylated alkylphenols; the structured liquid both suspends the hydrolase and protects the hydrolase against deactivation with water.

Abstract: Laundry additive compositions and methods for their use are provided. The compositions include a dispersible polyolefin, preferably polyethylene, and at least one compound selected from the group consisting of dye fixative agents, chelating agents, dye transfer inhibiting agents, chlorine scavenging agents, free radical scavenging agents, cellulase enzymes and mixtures thereof. Preferably, the composition is a liquid composition added to the rinse cycle of a consumer laundry process or to a pre-treatment cycle before a wash cycle of a consumer laundry process.

Abstract: There is provided a detergent composition comprising lipoxidase enzymes. The lipoxidase enzyme is preferably incorporated into the composition at a level of from 0.0001% to 2% active enzymes by weight of the composition.

Abstract: A two-part enzyme-based cleaning system useful in clean-in-place operations to remove proteinaceous soils from dairy equipment is described. The system comprises two liquid concentrates stored in separate containers, the concentrates being diluted and mixed for use.A. The first concentrate consists of:i) 1.5 to 7.5 percent by weight of a source of alkalinity selected from the sources of hydroxide based alkaline compositions;ii) 1 to 16 percent by weight of a water conditioner selected from the group consisting of polyacrylic acids and polyphosphates; andiii) balance water.B. The second concentrate consists of:i) 5 to 45 percent by weight of an enzyme stabilizing blend of an alkali salt of a (C.sub.6 to C.sub.12) fatty acid and a linear (C.sub.8 -C.sub.

Abstract: Enzyme compositions and methods employing enzyme compositions are disclosed which are useful for cleaning contact lenses. In one embodiment, a composition in accordance with the present invention comprises an enzyme component effective when released in a liquid medium to remove debris from a contact lens located in the liquid medium; and an activity regulating component effective when released in the liquid medium to deactivate the enzyme component located in the liquid medium. This composition is preferably structured so that the enzyme component is released in the liquid medium before the activity regulating component is so released. The period of time between the release of the enzyme component and the activity regulating component is sufficient to allow the enzyme component to effectively remove debris from a contact lens which is introduced into the liquid medium before or at the same time the enzyme component is released in the liquid medium.

Abstract: A method for stabilizing an enzyme against decomposition at elevated temperatures or by water is described which comprises combining the enzyme with stabilizing amounts of a non-ionic polyether-polyol block-copolymer surfactant. Stabilized compositions based on the enzyme and surfactant are also described.

Abstract: A method of cleaning soiled dishes comprising contacting said dishes with a composition comprising a lipased derived from pseudomonas pseudolacaligenes, a lime soap dispersant having an lime soapdispersing power of no more than eight, and a suds suppressing agent which contains either a silicone suds suppressing agent or a 2-alkyl alcanol suds suppressing agent.

Abstract: Methods for stabilizing enzymes in liquid compositions, including those liquid compositions having a high water content and those stabilized enzyme liquid compositions formed thereby. The method involves forming the enzyme, so that it is in an insoluble form thereof and then adding thereto an agent for maintaining the enzyme in the insoluble form thereof. Examples of such insoluble forms are crystal forms of the enzyme. Enzymes which are stabilized in this manner are useful for combining with liquid compositions, including liquid compositions having a high water content. The method is particularly useful for the preparation of stable enzyme-containing liquid detergent compositions.

Abstract: The present invention relates to a process for providing a bleached look in the color density of the surface of dyed fabric, especially cellulosic fabric such as denim, comprising use of a phenol oxidizing enzyme such as a peroxidase or a laccase, a hydrogen peroxide source and an enhancing agent represented by formula (I).

Abstract: A warewashing composition for a machine dishwasher and a method of using it is described. The composition comprises effective amounts of an organic peroxy acid, and an amylase enzyme which, when incubated at 55.degree. C. in a solution of 2 mM sodium citrate, 1 mM epsilon phthalimidoperoxyhexanoic acid in 36 ppm water at pH 8.0, has a half-life of two minutes or greater based on an activity vs. time plot obtained via monitoring color development at 405 nm of solution samples incubated with p-nitrophenyl-.alpha.-D-maltoheptaoside as substrate and gluco amylase and .alpha.-glucosidase as coupled enzymes; and 1% to 75% by weight of a builder. A 1% aqueous solution of the composition must have a pH of less than 10.

Abstract: Novel .alpha.-amylase enzymes are disclosed in which one or more asparagine residues are substituted with a different amino acid or deleted. The disclosed .alpha.-amylase enzymes show altered or improved low pH starch hydrolysis performance, stability and activity profiles.

Abstract: Low cost, highly aqueous, thickened heavy duty liquid laundry detergent compositions are provided. Such compositions contain relatively low levels of surfactant materials, a formate-based viscosity-enhancing agent, a selected type of thickening perfume and relatively large amounts of water. Only minimal amounts of other detergent composition adjuvants are permitted in such compositions.

Abstract: Mixed chain alkyl (alkyl glycosid) uronates as nonionic surfactants are disclosed.

Abstract: The present invention relates to a specific ternary enzyme stabilization system which unexpectedly enhances stability of a specific cellulase enzyme in isotropic liquid detergent compositions.

Abstract: This invention relates to enzymes, to rDNA techniques applicable for example to their production, to mutated genes, vectors and mutant and transformed microorganisms useful in their production, and to their uses including for example enzymatic detergent and cleaning compositions containing them.

Abstract: Compositions containing a stable, liquid, ophthalmically acceptable enzyme and methods involving the combined use of these compositions with a polymeric antimicrobial agent, and complexing agent are disclosed for the simultaneous cleaning and disinfecting of contact lens. Methods for a daily use regimen are also disclosed.

Abstract: A polyvinyl ether and paraffin wax blend is described which is useful as a coating for encapsulates which are stable in an alkaline environment and which exhibit a volume % compressibility of 20.degree. or less at 30.degree. C. The polyvinyl ether material has a formula [C.sub.x H.sub.2x O].sub.y.

Abstract: Low cost, effective aqueous heavy duty liquid laundry detergent compositions are provided. Such compositions contain relatively low levels of an anionic/nonionic-based suffactant system along with a protease-containing enzyme component, a low-cost viscosity-enhancing agent and relativly large amounts of water. The anionic component of the surfactant system comprises a combination of alkyl sulfate and alkyl ether sulfate. The nonionic component of the suffactant system comprises fatty alcohol ethoxylates. Only minimal amounts of other detergent composition adjuvants are permitted in such compositions.

Abstract: Aqueous liquid detergent compositions are described which comprise a proteolytic enzyme wherein the proteolytic activity is reversibly inhibited by an .alpha.-amino boronic acid.

Abstract: Compositions containing an ophthalmically acceptable enzyme of high purity and integrity in a liquid medium and methods involving the use of these compositions for cleaning a contact lens, and in combination with an antimicrobial agent for the simultaneous cleaning and disinfecting of contact lens are disclosed.

Abstract: Aqueous liquid detergent compositions are described which comprise a proteolytic enzyme wherein the proteolytic activity is reversibly inhibited by a peptide aldehyde.

Abstract: A two-part enzyme-based cleaning system useful in clean-in-place operations to remove proteinaceous soils from dairy equipment is described. The system comprises two liquid concentrates stored in separate containers, the concentrates being diluted and mixed for use.A. The first concentrate consists of:i) 2.5 to 7.5 percent by weight of a source of alkalinity selected from the sources of hydroxide based alkaline composition;ii) 1 to 16 percent by weight of a water conditioner selected from the group consisting of polyacrylic acids and polyphosphates; andiii) balance water.B. The second concentrate consists of:i) 35 to 45 percent by weight of a blend of an alkali salt of a (C.sub.6 to C.sub.12) fatty acid and a linear (C.sub.8 -C.sub.18) polyoxyalkylene;ii) 0.2 to 1.6 percent of a proteolytic enzyme; andiii) balance water.

Abstract: An alkaline protease which is suitable for detergent formulations is produced by three strains of a species of Bacillus proteolyticus. The alkaline protease has the amino acid terminal sequence of Seq. ID NO.: 1 as follows: Ala-Gln-Ser-Val-Pro-Trp-Gly-Ile-Ser-Arg-Val-Gln-Ala-Pro-Ala-Ala-His-Asn-Ar g-Gly-. In addition, the alkaline protease has a molecular weight of 28 kdaltons, an isoelectric point from 10-11.5, an optimum pH for proteolytic activity at a pH in the range of 8.5 and 11.5, and retains at least 70% of its original activity after being held at a pH of 8.0 at a temperature of 43.degree. C. for a period of 11 days.