Glycoprotein, E.g., Mucins, Proteoglycans, Etc. Patents (Class 530/395)
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Publication number: 20130035471Abstract: The present invention relates to a new streptavidin muteins. This mutein is The muteins are capable of oligomerization to form tetramers, with relatively strong subunit interactions, dissociation constant (KD) for biotin in this mutein in the range of 10?7 to 10?8M, off-rate (koff) for the bound biotin in the streptavidin-biotin complex in the range of 10?4 sec?1, stable enough to allow reuse, and producible producable with reasonable production yield via secretion in a soluble functional state without the requirement of refolding streptavidin via the tedious and expensive denaturation and renaturation processes.Type: ApplicationFiled: October 13, 2011Publication date: February 7, 2013Inventors: Sui-Lam Wong, Sau-Ching Wu, Isabelle Barrette-Ng, Ken Ng
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Patent number: 8367065Abstract: The present invention provides single chain antibody-directed polymeric prodrugs containing multifunctional linkers. Methods of making the polymeric delivery systems and methods of treating mammals using the same are also disclosed.Type: GrantFiled: March 12, 2009Date of Patent: February 5, 2013Assignee: Enzon Pharmaceuticals, Inc.Inventors: Hong Zhao, Prasanna Reddy, Maria Belen Rubio
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Publication number: 20130028924Abstract: Chimeric protein constructs including a herpesvirus glycoprotein D (gD) and a heterologous polypeptide that interact with herpes virus entry mediator (HVEM) and enhance and enhance an immune response against the heterologous polypeptide and methods for their use are provided.Type: ApplicationFiled: September 22, 2011Publication date: January 31, 2013Applicant: THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGYInventors: Hildegund C.J. ERTL, Marcio O. LASARO
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Publication number: 20130029396Abstract: The present disclosure relates to a multifunctional biomemory device in which a protein having a redox potential substituted with a metal ion is directly immobilized on a substrate. The present disclosure provides an operating method in which the redox state of the protein is controlled by applying three different potentials. The present disclosure provides a biomemory device in which the metal ion of a metalloprotein is substituted to allow for artificial control of the redox potential. The present disclosure provides a new-concept biomemory device as an information storage device based on the principle of electron transfer of a naturally occurring biomolecule.Type: ApplicationFiled: February 24, 2012Publication date: January 31, 2013Applicant: Industry-University Cooperation Foundation Sogang UniversityInventors: Jeong Woo Choi, Taek Lee, Jun Hong Min
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Publication number: 20130028926Abstract: The invention is directed to bioconjugate vaccines comprising N-glycosylated proteins. Further, the present invention is directed to a recombinant prokaryotic biosynthetic system comprising nucleic acids encoding an epimerase that synthesizes an oligo- or polysaccharide having N-acetylgalactosamine at the reducing terminus. The invention is further directed to N-glycosylated proteins containing an oligo- or polysaccharide having N-acetylgalactosamine at the reducing terminus and an expression system and methods for producing such N-glycosylated proteins.Type: ApplicationFiled: November 16, 2010Publication date: January 31, 2013Applicant: GLYCOVAXYN AGInventors: Michael Wacker, Charles Waechter
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Publication number: 20130029358Abstract: Immunostimulatory NY-ESO-1 epitopes recognized by MHC-DRB3*0202 (DR52b) or DRB1*0101 (DR1) restricted T cells are described. Methods for their use in diagnostic and therapeutic approaches are also provided. Further, methods for the generation and isolation of MHC class II molecules, either “empty” or peptide-loaded, are provided. Methods for the assembly of MHC class II multimers, for example, tetramers, are also provided. Methods for the detection of T cells binding to specific peptide-loaded MHC class II molecules are also described herein.Type: ApplicationFiled: October 1, 2010Publication date: January 31, 2013Applicant: LUDWIG INSTITUTE FOR CANCER RESEARCH LTD.Inventors: Danila Valmori, Maha Ayyoub, Immanuel F. Luescher, Danijel Dojcinovic
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Publication number: 20130029901Abstract: Bone targeted compounds and methods are provided. Compounds can include a Bone Targeting Portion (RT), having an affinity for bone; a Bone Active Portion (RA) for interacting with and affecting bone; and a Linking Portion (RL) connecting the Bone Targeting Portion and the Bone Active Portion.Type: ApplicationFiled: July 30, 2012Publication date: January 31, 2013Inventors: William M. PIERCE, JR., K. Grant TAYLOR, Leonard C. WAITE, Kevyn MERTEN
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Patent number: 8362224Abstract: This invention is directed to a marker gene for autoimmune disease. Specifically, the invention is directed to the use of a polymorph of CD93 in methods and compositions for the detection, prognosis and therapy of Type I Diabetes and systemic lupus erythematosus (SLE).Type: GrantFiled: December 19, 2007Date of Patent: January 29, 2013Assignee: The Trustees of the University of PennsylvaniaInventors: Hooman Noorchashm, Ali Naji
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Publication number: 20130023426Abstract: Methods and compositions for diagnosing the presence of a cancer cell in an individual are provided. Methods and compositions for identifying a tumor-specific signature in an individual having cancer are also provided. Methods and compositions for diagnosing the presence of an infectious agent in an individual and/or for identifying an infectious agent-specific signature in an infected individual are provided. Methods and compositions for diagnosing the presence of a disease in an individual are also provided. Methods and compositions for identifying a disease-specific signature in an individual having the disease are also provided.Type: ApplicationFiled: January 13, 2012Publication date: January 24, 2013Applicant: President and Fellows of Harvard CollegeInventor: Amin I. Kassis
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Publication number: 20130022968Abstract: Nucleic acid compositions, methods of making and using such compositions that comprise modular functional groups that can be configured to provide desired functionality to different nucleotide types, through a swappable and preferably non-covalent linkage component. Such compositions are useful in a variety of applications including nucleic acid analyses.Type: ApplicationFiled: July 19, 2012Publication date: January 24, 2013Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.Inventors: Jonas Korlach, Jeffrey Wegener
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Publication number: 20130022544Abstract: The present invention is directed to polymerized products and compositions useful for the treatment and prevention of amyloid disease in a subject. The invention further relates to isolated antibodies that recognize a common conformational epitope of amyloidogenic proteins or peptides that are useful for the diagnosis, treatment, and prevention of amyloid disease.Type: ApplicationFiled: July 16, 2012Publication date: January 24, 2013Applicant: NEW YORK UNIVERSITYInventors: Thomas M. Wisniewski, Fernando Goni
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Publication number: 20130023643Abstract: Disclosed herein are isolated peptides having nuclear localization activity and derived from the VP2 protein of chicken anemia virus (CAV). The isolated peptides are proven to be useful and effective in the nuclear delivery of a selected target substance, such as proteins, peptides, nucleic acids, pharmaceutically active agents, chemical substances, etc. The isolated peptide can transport a target substance, in particular a protein, into the nucleus of a mammalian cell by forming a conjugate with the target substance, or via an expression cassette capable of expressing a fusion protein having the isolated peptide and the target protein.Type: ApplicationFiled: July 20, 2012Publication date: January 24, 2013Applicant: China Medical UniversityInventors: Meng-Shiou Lee, Jai-Hong Cheng, Yi-Yang Lien
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Publication number: 20130018177Abstract: The present invention relates to eukaryotic host cells which have been modified to produce sialylated glycoproteins by the heterologous expression of a set of glycosyltransferases, including sialyltransferase and/or trans-sialidase, to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. Novel eukaryotic host cells expressing a CMP-sialic acid biosynthetic pathway for the production of sialylated glycoproteins are also provided. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities (such as those involved in sialylation) to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation.Type: ApplicationFiled: July 20, 2012Publication date: January 17, 2013Applicant: GlycoFi, Inc.Inventor: Stephen R. Hamilton
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Patent number: 8354247Abstract: A chemoenzymatic method for the preparation of a homogeneous glycoprotein or glycopeptide, including (a) providing an acceptor selected from the group consisting of GlcNAc-protein and GlcNAc-peptide; and (b) reacting the acceptor with a donor substrate including an activated oligosaccharide moiety, in the presence of a catalyst comprising endoglycosidase (ENGase), to transfer the oligosaccharide moiety to the acceptor and yield the homogeneous glycoprotein or glycopeptide. The donor substrate includes, in a specific implementation, a synthetic oligosaccharide oxazoline. A related method of glycoprotein or glycopeptide remodeling with a predetermined natural N-glycan or a tailor-made oligosaccharide moiety, and a method of remodeling an antibody including a heterogeneous sugar chain, are also described. The disclosed methodology enables glycoprotein drugs to be modified for prolonged half-life in vivo, reduced immunogenicity, and enhanced in vivo activity, and for targeting and drug delivery.Type: GrantFiled: October 5, 2010Date of Patent: January 15, 2013Assignee: University of Maryland, BaltimoreInventor: Lai-Xi Wang
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Publication number: 20130011432Abstract: The invention is directed to an adenovirus-antigen conjugate comprising (a) a disrupted adenovirus with a coat protein and (b) an antigen conjugated to the coat protein of the disrupted adenovirus, as well as a conjugate comprising (a) a disrupted adenovirus with a coat protein and (b) an antigen conjugated to the coat protein of the disrupted adenovirus. The invention also provides a method of inducing an immune response against an antigen in a human using the aforementioned conjugates. The invention further provides an adeno-associated viral vector comprising a nucleic acid sequence which encodes an antibody directed against cocaine.Type: ApplicationFiled: March 17, 2011Publication date: January 10, 2013Applicant: CORNELL UNIVERSITY (CCTEC)Inventors: Ronald G. Crystal, Bishnu De, Martin Hicks, Jonathan Rosenberg, Stephen M. Kaminsky
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Publication number: 20130012435Abstract: The present invention relates to multivalent polypeptides comprising at least two fibronectin scaffold domains connected via a polypeptide linker. The invention also relates to multivalent polypeptides for use in diagnostic, research and therapeutic applications. The invention further relates to cells comprising such proteins, polynucleotide encoding such proteins or fragments thereof, and to vectors comprising the polynucleotides encoding the innovative proteins.Type: ApplicationFiled: June 26, 2012Publication date: January 10, 2013Applicant: Bristol-Myers Squibb CompanyInventors: Ray Camphausen, Eric Furfine, Irvith M. Carvajal, H. Nicholas Marsh, Marco Gottardis, Joan Carboni, Ricardo Attar
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Publication number: 20130012453Abstract: The present invention relates to fusion constructs of glycosylphosphatidylinositol (GPI)-anchored tissue inhibitors of metalloproteinases (TIMPs) and their use for the treatment of cancer and in regenerative medicine. By this approach, the GPI-anchored TIMP proteins are incorporated into the surface membrane of cells. The fusion constructs of the present invention are effective agents in accelarating wound healing.Type: ApplicationFiled: November 22, 2010Publication date: January 10, 2013Applicant: GMG Beratungs-und Beteiligungs Verwaltungs BmbHInventor: Peter Jon Nelson
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Publication number: 20130005661Abstract: The present invention discloses a pharmaceutical polymer and a method for quenching free radicals. Said pharmaceutical polymer comprises a glycopeptides and an aminothiol moiety which covalently bonds together. The disclosed pharmaceutical polymer and method can be applied before or after the occurrence of radiation exposure.Type: ApplicationFiled: May 30, 2012Publication date: January 3, 2013Applicant: TAIWAN HOPAX CHEMS. MFG. CO., LTD.Inventors: Chau-Hui Wang, Chia-Hung Chen, Jing-Yi Chen, Chih-Wei Hsu
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Publication number: 20130005945Abstract: Scaffold comprises a polymer defining macropores and comprising hydroxypropylcellulose partially substituted by a substituent comprising a self-crosslinkable group, which is crosslinked through the self-crosslinkable group. The macropores have an average pore size larger than 50 microns and are at least partially interconnected. In one method, bicontinuous emulsion comprising a continuous aqueous phase and a continuous polymer phase is formed. The polymer phase comprises hydroxypropylcellulose partially substituted by a substituent comprising a self-crosslinkable group, and is crosslinked through the self-crosslinkable group to form a polymer defining at least partially interconnected pores. In another method, phase separation is induced in a solution comprising a polymer precursor and water to form a bicontinuous emulsion comprising a continuous polymer phase and a continuous aqueous phase.Type: ApplicationFiled: September 12, 2012Publication date: January 3, 2013Inventors: Zhilian Yue, Feng Wen, Hanry Yu
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Publication number: 20130004980Abstract: A regulatable gene expression construct comprising a nucleic acid molecule comprising a two-way riboswitch operably linked to a target sequence. Also provided is a library screening strategy for efficient creation of target-specific riboswitches. A theophylline-repressible and IPTG-inducible riboswitch device achieves portable control of gene expression control in a ‘two-way’ manner. The default state of target genes is ON; the targets are switched off by adding theophylline, and switched back to the ON-state by adding IPTG without changing growth medium. The riboswitch device regulates gene expression in a portable, adjustable, and two-way manner with a variety of scientific and biotechnological applications.Type: ApplicationFiled: June 27, 2012Publication date: January 3, 2013Applicant: UNIVERSITY OF HONG KIONGInventors: Jian-Dong Huang, Ye Jin
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Publication number: 20130004543Abstract: Isolated protein complexes are provided comprising growth factors such as IGF-I, IGF-II, EGF, bFGF, or KGF and fibronectin, or at least domains thereof that enable binding to and activation of both a growth factor receptor, and an integrin receptor-binding domain of fibronectin. These protein complexes include synthetic proteins where the growth factor and fibronectin sequences are joined by a linker sequence. Also provided are uses of these protein complexes for stimulating or inducing cell migration and/or proliferation in wound healing, tissue engineering, cosmetic and therapeutic treatments such as skin replacement, skin replenishment and treatment of burns where epithelial cell migration is required. In other embodiments, the invention provides inhibition of cancer cell metastasis, particularly in relation to breast cancer.Type: ApplicationFiled: November 30, 2010Publication date: January 3, 2013Applicant: Queensland University of TechnologyInventors: Zee Upton, Derek Van Lonkhuyzen
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Publication number: 20120328628Abstract: The present invention provides methods for generating antibodies to specific conformations of proteins. The conformation specific antibodies of the invention can be put to a variety of uses including diagnosis and treatment of diseases and for screening for compounds that induce conformational changes in proteins upon binding.Type: ApplicationFiled: July 6, 2007Publication date: December 27, 2012Applicant: The Regents of the University of CaliforniaInventors: James A. Wells, Junjun Gao
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Publication number: 20120328641Abstract: Stabilized forms of gp120 polypeptide, nucleic acids encoding these stabilized forms, vectors comprising these nucleic acids, and methods of using these polypeptides, nucleic acids, vectors and host cells are disclosed. Crystal structures and computer systems including atomic coordinates for stabilized forms of gp120, and gp120 with an extended V3 loop, and methods of using these structures and computer systems are also disclosed.Type: ApplicationFiled: August 14, 2012Publication date: December 27, 2012Inventors: Peter Kwong, John Mascola, Gary Nabel, Richard Wyatt, Barna Dey, Ling Xu, Tongqing Zhou, Joseph Sodroski, Wen Yuan, Shi-Hua Xiang
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Publication number: 20120328632Abstract: This invention relates to the inhibition of intercellular adhesion mediated by L-selectin by administering a newly identified L-selectin ligand, CD34. More particularly, the invention concerns a method for inhibiting leukocyte adhesion to endothelial cells by administering an effective amount of an isolated, purified CD34 polypeptide or an antibody capable of binding native CD34.Type: ApplicationFiled: December 21, 2011Publication date: December 27, 2012Applicant: Genentech, Inc.Inventors: Laurence A. Lasky, Susanne Baumhueter, Steven D. Rosen, Mark S. Singer
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Publication number: 20120328560Abstract: Provided is a method of obtaining biologically active recombinant human G-CSF from inclusion bodies, wherein the solubilization and refolding process can be performed at ambient temperature and the purification step comprises reversed phase chromatography (RP), in particular RP-HPLC. The G-CSF preparation so obtained is characterized by high purity and homogeneity.Type: ApplicationFiled: March 17, 2011Publication date: December 27, 2012Applicant: BIOGENERIX AGInventors: Walter Hinderer, Christian Scheckermann
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Publication number: 20120329129Abstract: A magnetic nanoparticle is provided in the disclosure. The magnetic nanoparticle includes a magnetic nanoparticle; a biocompatible polymer of the following formula (II) covalently coupled to the magnetic nanoparticle, wherein R1 is alkyl, aryl, carboxyl, or amino; n is an integer from 5 to 1000; and m is an integer from 1 to 10.Type: ApplicationFiled: August 30, 2012Publication date: December 27, 2012Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Wen-Hsiang Chang, Wen-Uan Hsieh, Shiu-Hua Huang, Chin-I Lin, Shian-Jy Jassy Wang, Kelly Teng
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Publication number: 20120328519Abstract: The present invention relates to the novel compound classes of dendritic polyglycerol sulfates and sultanates as well as to their production and use for the treatment of diseases, particularly inflammatory diseases, and to their use as selectin inhibitors and selectin indicators. The dendritic polyglycerol sulfates and sulfonates are also suitable for imaging diagnostics, particularly with respect to inflammatory diseases.Type: ApplicationFiled: August 3, 2007Publication date: December 27, 2012Inventors: Rainer Haag, Jens Dernedde, Rudolf Tauber, Gesche Bernhard, Sven Enders, Heidemarie Weinhart, Arne Von Bonin, Ulrich Zügel, Holger Türk
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Publication number: 20120329092Abstract: The present invention relates to a process for the purification of a glycoprotein comprising subjecting a liquid containing said glycoprotein to the steps of: a) reverse phase chromatography, b) boronate affinity chromatography, and c) size exclusion chromatography. Also provided is a manufacturing process for producing a glycoprotein of interest.Type: ApplicationFiled: January 25, 2011Publication date: December 27, 2012Applicant: Glycotope GmbHInventors: Steffen Goletz, Lars Stöckl
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Publication number: 20120329127Abstract: The present disclosure relates to materials and methods of conjugating a water soluble polymer to a therapeutic protein.Type: ApplicationFiled: May 25, 2012Publication date: December 27, 2012Applicants: BAXTER HEALTHCARE S.A., BAXTER INTERNATIONAL INC.Inventors: Juergen Siekmann, Alfred Weber, Hanspeter Rottensteiner, Peter Turecek
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Patent number: 8338183Abstract: Disclosed relates to an electrochemical determination system of glycated proteins, the system comprising: a filtering means for filtering labeled compounds bound to glycated proteins and non-glycated proteins after adding labeling compounds, capable of selectively binding to the glycated proteins to a solution, in which glycated/non-glycated proteins coexist, to be bound all to the glycated proteins; and a quantifying means for quantifying the filtered labeling compounds, not bound to the glycated proteins. The system of the present invention filters the residual labeled compounds left after binding to glycated proteins to quantify, instead of directly quantifying glycated proteins via the known glycated protein determination methods, thus simplifying the configuration of the system that can provide exact determinations with a low cost.Type: GrantFiled: July 29, 2006Date of Patent: December 25, 2012Assignee: i-SENS, Inc.Inventors: Hakhyun Nam, Fenghua Zhang, Soon Hye Yang, Bo Kyeong Kang, Gang Cui, Moon Hee Choi, Joung Su Lee, Geun Sig Cha
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Publication number: 20120321660Abstract: Process for conjugation of bacterial saccharides including Streptococcus pneumoniae and Haemophilus influenzae saccharides by reductive amination are provided herein.Type: ApplicationFiled: March 7, 2011Publication date: December 20, 2012Inventors: Ralph Leon Biemans, Pierre Duvivier, Ollivier Francis Nicolas Gavard
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Publication number: 20120322684Abstract: Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker.Type: ApplicationFiled: July 11, 2012Publication date: December 20, 2012Inventors: Sang Yup Lee, Nae Choon Yoo, So Young Yoo, Hyun Cheol Chung, Ki Chang Keum, Won Min Yoo, Ki Jun Jeong
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Publication number: 20120321659Abstract: This invention relates to hepatitis B virus (“HBV”) core antigen particles that are characterized by multiple immunogen specificities. More particularly, the invention relates to HBV core antigen particles comprising immunogens, epitopes, or other related structures, crosslinked thereto by ligands which are HBV capsid-binding peptides that selectively bind to HBV core protein. Such particles may be used as delivery systems for a diverse range of immunogenic epitopes, including the HBV capsid-binding peptides, which advantageously also inhibit and interfere with HBV viral assembly by blocking the interaction between HBV core protein and HBV surface proteins. Mixtures of different immunogens and/or capsid-binding peptide ligands may be crosslinked to the same HBV core particle. Such resulting multicomponent or multivalent HBV core particles may be advantageously used in therapeutic and prophylactic vaccines and compositions, as well as in diagnostic compositions and methods using them.Type: ApplicationFiled: April 26, 2012Publication date: December 20, 2012Applicant: BIOGEN IDEC MA INC.Inventor: Kenneth Murray
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Publication number: 20120321658Abstract: The present invention relates to an immunogenic composition comprising at least 2 different S. pneumoniae capsular saccharides, wherein one or more is/are selected from a first group consisting of serotypes 1, 3, 19A and 19F which is/are linked to a protein carrier(s) either directly or indirectly through a chemistry other than reductive amination, and one or more different saccharides is/are selected from a second group consisting of serotypes 4, 5, 6A, 6B, 7F, 9V, 14, 18C and 23F which is/are linked to a protein carrier(s) by reductive amination. Uses of such compositions are also disclosed.Type: ApplicationFiled: August 17, 2010Publication date: December 20, 2012Inventors: Ralph Leon Biemans, Pierre Duvivier, Ollivier Francis Nicolas Gavard, Jan Poolman
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Publication number: 20120315685Abstract: Disclosed herein are coated articles and methods of preparing the same.Type: ApplicationFiled: August 20, 2012Publication date: December 13, 2012Applicant: ECOLOGY COATINGS, INC. A Nevada CorporationInventor: Sally Judith Weine Ramsey
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Patent number: 8329659Abstract: Polypeptides are susceptible to denaturation or enzymatic degradation in the blood, liver or kidney. Due to the low stability of some polypeptides, it has been required to administer polypeptide drugs in a sustained frequency to a subject in order to maintain an effective plasma concentration of the active substance. Furthermore, pharmaceutical compositions of therapeutic peptides preferably have a shelf-life of several years in order to be suitable for common use. However, peptide compositions are inherently unstable due to sensitivity towards chemical and physical degradation. In part, the invention provides SAP variant proteins, compositions, pharmaceutical preparations and formulations having a prolonged in vivo half-life, prolonged shelf-life, or rather increased in vitro stability, or increased manufacturing efficiency compared to human SAP. Advantages of increased plasma half-life include, but are not limited to, reducing the amount and/or frequency of dosing.Type: GrantFiled: June 17, 2010Date of Patent: December 11, 2012Assignee: Promedior, Inc.Inventor: W. Scott Willett
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Patent number: 8329653Abstract: The invention provides methods and compositions for reducing or inhibiting net beta-amyloid peptide production and/or amyloid plaque formation. The methods and compositions involve administering a netrin-1 polypeptide or netrin-1 therapeutic to a subject in need thereof.Type: GrantFiled: April 16, 2007Date of Patent: December 11, 2012Assignees: Buck Institute for Age Research, Centre National de la Recherche ScientifiqueInventors: Patrick Mehlen, Dale E. Bredesen, Filipe Calheiros-Lourenco, Veronica Galvan
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Publication number: 20120308600Abstract: E. coli proteins have been identified that are useful as carrier proteins to improve a response to a polysaccharide immunogen conjugated to such protein. In particular, AcfD precursor protein (orf3526 polypeptide), Flu antigen 43 protein (orf1364 polypeptide), and Sel1 repeat-containing protein (upec-5211 polypeptide) have been shown to be effective. Additionally, these E. coli proteins can enhance the immune response to glucans, particularly fungal glucans.Type: ApplicationFiled: December 30, 2010Publication date: December 6, 2012Applicant: Novartis AGInventors: Paolo Costantino, Francesco Berti, Francesca Micoli, Anna Kasanova, Laura Serino, Maria Rosaria Romano, Marta Tortini
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Publication number: 20120309936Abstract: Disclosed are compositions and methods for producing fusion proteins with reduced immunogenicity. Fusion proteins of the invention include a junction region having an amino acid change that reduces the ability of a junctional epitope to bind to MHC Class II, thereby reducing its interaction with a T-cell receptor. Methods of the invention involve analyzing, changing, or modifying one or more amino acids in the junction region of a fusion protein in order to identify a T-cell epitope and reduce its ability to interact with a T cell receptor. Compositions and methods of the invention are useful in therapy.Type: ApplicationFiled: May 26, 2011Publication date: December 6, 2012Applicant: Merck Patent GmbHInventors: Stephen D. Gillies, Jeffrey Way, Anita A. Hamilton
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Publication number: 20120301480Abstract: Dextrans produced by lactic acid bacteria Leuconostoc mesenteroides and consisting of linear ?1,6-glucan chains modified with short side-chains composed of consecutive ?1,6-linked glucose residues were linked to a carrier protein. Three dextrans, namely Dextran-5K, Dextran-3.5K and Dextran-1.5K, with molecular masses of 5,000 Da, 3,500 Da and 1,500 Da, respectively, were modified with a diamino group-containing linker, followed by the introduction of maleimido functionality and conjugation to thiolated tetanus toxoid (TT), yielding Dextran-5K-TT, Dextran-3.5K-TT and Dextran-1.5K-TT conjugates. Studies performed with post-immune sera of mice and rabbits immunized with dextran-based glycococonjugates demonstrated cross-reactivity with LPS from typeable and non-typeable H. pylori strains and selected mutants. The post-immune sera from rabbits that received the conjugates exhibited functional activity against ?1,6-glucan-positive strains of H.Type: ApplicationFiled: January 10, 2012Publication date: November 29, 2012Inventors: Eleanora Altman, Blair A. Harrison, Vandana Chandan
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Publication number: 20120302512Abstract: The present disclosure provides isolated laminin-421, methods for making recombinant laminin-421, and host cells that express recombinant laminin-421. The present disclosure also provides nucleic acid sequences encoding full length human laminin ?2 chain, expression vectors and host cells thereof.Type: ApplicationFiled: March 1, 2012Publication date: November 29, 2012Applicant: BioLamina ABInventors: Karl Tryggvason, Sergey Rodin
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Publication number: 20120301457Abstract: The present invention embraces methods and kits for facilitating the propogation of PrPSc, and use of the same in increasing the sensitivity diagnostic assays and in identifying compounds that modulate the conversion of PrPc to PrPSc.Type: ApplicationFiled: January 19, 2011Publication date: November 29, 2012Inventors: Surachai Supattapone, Nathan R. Deleault
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Publication number: 20120301962Abstract: Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented.Type: ApplicationFiled: May 4, 2012Publication date: November 29, 2012Inventors: James A. Thomson, Zhonggang Hou, Guokai Chen
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Publication number: 20120295964Abstract: Provided is a lentiviral vector system which sustains a high-frequency retrograde transportation ability in animal brain and has a higher titer. A kit for preparing a retrograde transport viral vector, which comprises: (1) a packaging plasmid containing the gag gene and pol gene of HIV-1; (2) a packaging plasmid containing an accessory gene of HIV-1; (3) a transfer plasmid containing a target gene; and (4) an envelope plasmid containing, as an envelope gene, a gene encoding a fused polypeptide comprising the extracellular domain of rabies virus glycoprotein (RV-G), the transmembrane domain of rabies virus glycoprotein (RV-G) or vesicular stomatitis virus glycoprotein (VSV-G) and the intracellular domain of vesicular stomatitis virus glycoprotein (VSV-G).Type: ApplicationFiled: November 11, 2010Publication date: November 22, 2012Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventor: Kazuto Kobayashi
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Publication number: 20120295287Abstract: This document provides methods and materials related to detecting neurotransmitters and other biologically active small molecules. For example, methods for detecting and measuring hapten levels in a biological sample using antibodies specific for conjugated haptens are provided.Type: ApplicationFiled: November 8, 2010Publication date: November 22, 2012Applicant: Pharmasan Labs, Inc.Inventors: Gottfried H. Kellermann, Han J.G. Huisman
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Publication number: 20120288518Abstract: This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.Type: ApplicationFiled: December 17, 2010Publication date: November 15, 2012Applicant: Fina Bio Solutions ,LLCInventor: Andrew Lees
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Publication number: 20120288935Abstract: Modified nanoparticles are disclosed. More specifically, nanoparticles modified with an agent through a triazole linkage are disclosed. Also disclosed are methods of preparing modified nanoparticles and methods of using these modified nanoparticles.Type: ApplicationFiled: September 22, 2010Publication date: November 15, 2012Applicant: NORTHWESTERN UNIVERSITYInventors: Chad A. Mirkin, Joshua I. Cutler
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Publication number: 20120283420Abstract: The invention provides methods for producing multi-antennary glycoproteins in plant and plant cells. In particular the invention provides plants comprising a chimeric gene comprising glucosaminyltransferase IV and plants comprising two chimeric genes comprising glucosaminyltransferase IV and V.Type: ApplicationFiled: April 19, 2010Publication date: November 8, 2012Inventors: Bieke Nagels, Koen Weterings
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Publication number: 20120282285Abstract: The present invention relates to novel ficolin-associated polypeptides, and polypeptides derived from these ficolin-associated polypeptides for the use in the treatment of conditions associated with inflammation, apoptosis, autoimmunity, coagulation, thrombotic or coagulopathic related diseases, as well as the use as biomarkers. The present invention further relates to anti-bodies recognising such novel ficolin-associated polypeptides, and polypeptides derived thereof, nucleic acid molecules encoding such polypeptides, vectors and host cells used in the production of the polypeptides.Type: ApplicationFiled: July 16, 2010Publication date: November 8, 2012Applicants: RIGSHOSPITALET, SYDDANSK UNIVERSITET, KØBENHAVNS UNIVERSITETInventors: Peter Garred, Tina Hummelshøj Glue, Mikkel-Ole Skjødt
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Patent number: 8303956Abstract: The present invention is directed to ligand/receptor and antigen/antibody specificity exchangers comprising a saccharide or glycoconjugate. Methods of making these specificity exchangers and methods of using said specificity exchangers to treat or prevent human disease are described herein.Type: GrantFiled: March 26, 2010Date of Patent: November 6, 2012Assignee: Chrontech Pharma ABInventors: Matti Sällberg, Anders Vahlne, Maria Perdomo