Glycoprotein Hormones Patents (Class 530/397)
  • Patent number: 10155046
    Abstract: Derivatives of PSAs are synthesised, in which a reducing and/or non-reducing end terminal sialic acid unit is transformed into a N-hydroxysuccinimide (NHS) group. The derivatives may be reacted with substrates, for instance substrates containing amine or hydrazine groups, to form non-cross-linked/crosslinked polysialylated compounds. The substrates may, for instance, be therapeutically useful drugs, peptides or proteins or drug delivery systems.
    Type: Grant
    Filed: December 19, 2015
    Date of Patent: December 18, 2018
    Assignee: Lipoxen Technologies Limited
    Inventors: Sanjay Jain, Ioannis Papaioannou, Smita Thobhani
  • Patent number: 10155045
    Abstract: Derivatives of PSAs are synthesized, in which a reducing and/or non-reducing end terminal sialic acid unit is transformed into a N-hydroxysuccinimide (NHS) group. The derivatives may be reacted with substrates, for instance substrates containing amine or hydrazine groups, to form non-cross-linked/crosslinked polysialylated compounds. The substrates may, for instance, be therapeutically useful drugs, peptides or proteins or drug delivery systems.
    Type: Grant
    Filed: December 19, 2015
    Date of Patent: December 18, 2018
    Assignee: Lipoxen Technologies Limited
    Inventors: Sanjay Jain, Ioannis Papaioannou, Smita Thobhani
  • Patent number: 9920107
    Abstract: The present invention refers to a method of purification of HMG (human menopausal gonadotropin) by multimodal chromatography in order to obtain HMG-UP (human menopausal gonadotropin with ultra-purity grade) composition.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: March 20, 2018
    Assignees: INSTITUTO MASSONE S.A., RAUL ENRIQUE MASSONE
    Inventors: Liliana Balanián, Mariana Cancela, Claudio Wolfenson, José Groisman
  • Patent number: 9701730
    Abstract: The present invention relates to the field of Biotechnology and more particularly to the mutated polypeptides of TGF? molecule whose primary sequence has a high homology with the sequence of human TGF?. These muteins lose their ability to interact with ALK5 but maintain their ability to interact with other receptors that are part of the receptor complex (T?RII and T?RIII). They have the property of antagonizing the signaling of all natural variants of TGF? ligands, dependent of ALK5 recruitment in the receptor complex, and have significant immunomodulatory effects. The present invention relates to pharmaceutical compositions comprising as active principle the polypeptides or fusion proteins disclosed and their use thereof given their modulating effect on the immune system in diseases such as cancer, diseases accompanied by fibrosis and chronic infectious diseases.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: July 11, 2017
    Assignee: Centro De Inmunologia Molecular
    Inventors: Ángel de Jesús Corria Osorio, Kalet León Monzón, Tania Carmenate Portilla, Amaury Pupo Meriño, Saumel Pérez Rodríguez
  • Patent number: 9320777
    Abstract: Methods and compositions for the use of long-acting hematopoietic factor protein analogs for accelerating hematopoietic recovery in subjects who have been or will be exposed to radiation are disclosed.
    Type: Grant
    Filed: May 14, 2012
    Date of Patent: April 26, 2016
    Assignee: Bolder Biotechnology, Inc.
    Inventors: George N. Cox, Christie M. Orschell
  • Publication number: 20150119325
    Abstract: The present invention provides methods for the high-level production of recombinant human erythropoietin (rhuEPO) derivative, asialoerythropoietin (asialo-rhuEPO), in plants. The methods for producing asialo-rhuEPO comprise making a plant or at least one plant cell that comprises a promoter that drives expression in a plant cell operably linked to a polynucleotide encoding a human erythropoieting fusion protein and a promoter that drives expression in a plant cell operably linked to a polynucleotide encoding N-glycosylation modification enzyme, particularly a mammalian ?1,4-galactosyltransferase. The present invention further provides plants, plant cells, and seeds that have been genetically modified to produce high levels of asialo-rhuEPO. Additionally, provided are methods for purifying asialo-rhuEPO from plant tissues.
    Type: Application
    Filed: March 14, 2013
    Publication date: April 30, 2015
    Applicant: NORTH CAROLINA CENTRAL UNIVERSITY
    Inventors: Jiahua Xie, Farooqahmed S. Kittur, Chiu-Yueh Hung
  • Patent number: 8993609
    Abstract: The present invention relates to polyamide compositions and therapies for treating cells and subjects infected with papilloma virus.
    Type: Grant
    Filed: January 25, 2012
    Date of Patent: March 31, 2015
    Assignees: Nanovir, LLC, The Curators of the University of Missouri
    Inventors: James K. Bashkin, Kevin J. Koeller, Terri Grace Edwards, Christopher Fisher
  • Patent number: 8993732
    Abstract: The present invention, pertains in general to the field of stabilization of FSH formulations, in particular liquid FSH formulations. The stabilization is achieved by the addition of salts comprising pharmaceutically acceptable alkali metal cations, in preferred embodiments by the addition of pharmaceutically acceptable salts, i.e. sodium salts or potassium salts.
    Type: Grant
    Filed: July 28, 2011
    Date of Patent: March 31, 2015
    Assignee: Ferring B.V.
    Inventors: Helen Ulrika Sjögren, Heidi Louise Bagger
  • Publication number: 20150056254
    Abstract: A transdermal delivery system of drug and a method of preparing the same are provided. More specifically, the invention can be a transdermal delivery system applied for various protein drugs such as vaccines and chemical drugs, because the drug delivery system has a biocompatibility, biodegradation property, transdermal delivery property, the safety of human body, maximum activity of protein drugs, good bio-conjugation efficiency and a long-term efficacy, a method of preparing the same and its use.
    Type: Application
    Filed: February 7, 2013
    Publication date: February 26, 2015
    Applicant: PHI BioMed Co., Ltd.
    Inventors: Sei Kwang Hahn, Eung-Sam Kim, Jeonga Yang, Hyemin Kim, Kwan Yong Choi, Ji Hye Shin, Jung-Hee Kwon
  • Patent number: 8962811
    Abstract: Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.
    Type: Grant
    Filed: April 3, 2013
    Date of Patent: February 24, 2015
    Assignee: Ohio University
    Inventors: Marcia J. Kieliszewski, Jianfeng Xu, John Kopchick, Shigeru Okada, Gary Meyer
  • Patent number: 8940876
    Abstract: The present disclosure relates to a method for preparing recombinant glycoproteins with high sialic acid content. More specifically, for UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE/MNK) enzyme where point mutation was induced by substituting arginine at position 263 by leucine only or by further substituting arginine at position 266 by glutamine, epimerase activity is constantly maintained, and overexpressed cells thereof experience an increase in intracellular cytidine monophosphate (CMP)-sialic acid content, irrespective of CMP-sialic acid concentration.
    Type: Grant
    Filed: February 1, 2011
    Date of Patent: January 27, 2015
    Assignee: Korea Advanced Institute of Science and Technology
    Inventors: Jung Hoe Kim, Young Dok Son, Jin Young Hwang, Yeon Tae Jeong
  • Publication number: 20150024455
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Application
    Filed: July 17, 2014
    Publication date: January 22, 2015
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • Publication number: 20150018274
    Abstract: The present invention provides isolated stabilized EPO-derived peptides and their mimics that protect against tissue damage in subjects having diverse forms of neural and non-neural organ system injury, pharmaceutical compositions containing the isolated stabilized EPO-derived peptides, methods for treating symptoms of a disease, disorder or condition having an inflammatory or an autoimmune component in a subject in need thereof, and methods for downregulating immune mediator activity in a subject in need thereof.
    Type: Application
    Filed: January 23, 2014
    Publication date: January 15, 2015
    Applicant: UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY
    Inventors: Yasuhiro Maeda, Rui Rong Yuan, Joseph Menonna, Wei Ping Li, Peter Dowling
  • Publication number: 20150010494
    Abstract: A method of preparing an oxidised polysaccharide-protein conjugate by oxidising a polysaccharide with an oxidising agent to form an oxidised polysaccharide and combining such oxidised polysaccharide with a protein. The oxidised polysaccharide is reacted with a protein to form a composition comprising a conjugate wherein the oxidised polysaccharide and the protein are conjugated via one or more imine bonds and wherein the oxidised polysaccharide comprises essentially no alpha-hydroxy aldehyde units. The conjugate may be used to provide sustained or latent activity of the protein.
    Type: Application
    Filed: September 22, 2014
    Publication date: January 8, 2015
    Inventors: Gord Adamson, David Bell, Steven Brookes
  • Publication number: 20140357846
    Abstract: Described herein are compositions of Thyroid Stimulating Hormone (TSH), wherein at least one polyalkylene glycol polymer is attached to a carbohydrate site of the TSH. Also described are compositions of mutated Thyroid Stimulating Hormone (TSH) and at least one polyalkylene glycol polymer, wherein the mutated TSH comprises a TSH in which one or more amino acid residues has been substituted with cysteine residue, and the polyalkylene glycol polymer is attached to the mutated TSH at the site of the substituted cysteine residue. Pharmaceutical compositions comprising these TSH compositions and method of treating a thyroid condition in a patient in need thereof, by administering to the patient an effective amount of the pharmaceutical compositions are also described.
    Type: Application
    Filed: June 18, 2014
    Publication date: December 4, 2014
    Inventors: Clark PAN, Huawei QIU, Sunghae PARK
  • Patent number: 8889837
    Abstract: The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
    Type: Grant
    Filed: January 24, 2011
    Date of Patent: November 18, 2014
    Assignee: Hoffman-La Roche Inc.
    Inventors: Josef Burg, Klaus Reichert, Axel Schroth, Hartmut Schurig, Axel Wessner
  • Publication number: 20140336117
    Abstract: We disclose growth hormone fusion proteins that have increased in vivo stability and activity; nucleic acid molecules encoding said proteins and methods of treatment of growth hormone related diseases that would benefit from growth hormone agonists or antagonists.
    Type: Application
    Filed: March 14, 2012
    Publication date: November 13, 2014
    Applicant: ASTERION LIMITED
    Inventors: Peter Artymiuk, Ian Wilkinson, Richard Ross
  • Publication number: 20140323324
    Abstract: The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.
    Type: Application
    Filed: December 16, 2011
    Publication date: October 30, 2014
    Applicant: National Institute of Advanced Industrial Science and Technology
    Inventors: Hisashi Narimatsu, Jun Hirabayashi, Atsushi Kuno, Hideki Matsuzaki, Yuzuru Ikehara, Hiromi Ito, Yasuhiro Hashimoto, Keiro Shirotani, Satoshi Futakawa, Hajime Arai, Masakazu Miyajima, Kazuo Fujihara
  • Publication number: 20140308744
    Abstract: Compositions of purified biologically active Wnt proteins are provided. Wnt proteins are found to be hydrophobic and post-translationally modified by addition of a lipid moiety at a conserved cysteine residue. Methods for isolation of Wnt utilize detergents that maintain the solubility of the modified protein.
    Type: Application
    Filed: January 16, 2014
    Publication date: October 16, 2014
    Inventors: Roeland Nusse, Karl H. Willert
  • Publication number: 20140309407
    Abstract: The present invention relates to a compound which is a polysaccharide derivative of EPO, or of an EPO like protein, wherein the polysaccharide is anionic and comprises between 2 and 200 saccharide units. The present invention also relates to pharmaceutical compositions comprising the novel compounds, and methods for making the novel compounds.
    Type: Application
    Filed: June 24, 2014
    Publication date: October 16, 2014
    Applicant: LIPOXEN TECHNOLOGIES LIMITED
    Inventors: Sanjay Jain, Peter Laing, Gregory Gregoriadis, Norbert Oskar Rumpf
  • Publication number: 20140295521
    Abstract: A new use of a molecule comprising at least one moiety which is a biologically active protein and at least one moiety capable of binding to a serum albumin of a mammal is provided, for preparation of a medicament which elicits no or a reduced immune response upon administration to the mammal, as compared to the immune response elicited upon administration to the mammal of the biologically active protein per se. Also provided is a method of reducing or eliminating the immune response elicited upon administration of a biologically active protein to a human or non-human mammal, which comprises coupling the polypeptide to at least one moiety capable of binding to a serum albumin of the mammal.
    Type: Application
    Filed: January 21, 2014
    Publication date: October 2, 2014
    Applicant: AFFIBODY AB
    Inventor: Nina Herne
  • Publication number: 20140294762
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Application
    Filed: April 7, 2014
    Publication date: October 2, 2014
    Applicant: Novo Nordisk A/S
    Inventors: Shawn DeFrees, David A. Zopf, Robert J. Bayer, David James Hakes, Caryn L. Bowe, Xi Chen
  • Publication number: 20140256622
    Abstract: The disclosure provides cross-linked materials that include multivalent cross-linking agents that bind an exogenous target molecule; and conjugates that include two or more separate affinity ligands bound to a conjugate framework, wherein the two or more affinity ligands compete with the exogenous target molecule for binding with the cross-linking agents and wherein conjugates are cross-linked within the material as a result of non-covalent interactions between cross-linking agents and affinity ligands on different conjugates. The conjugates also include a drug.
    Type: Application
    Filed: May 1, 2014
    Publication date: September 11, 2014
    Applicant: SmartCells, Inc.
    Inventors: Todd C. Zion, Thomas M. Lancaster
  • Publication number: 20140248234
    Abstract: The invention discloses the use of the G-CSF dimer in the preparation of a medicament for the treatment of neurodegenerative diseases. Use of the G-CSF dimer of the present invention can significantly increase the number of dopaminergic neuron in the substantia nigra in PD model animals and enhance the function of dopaminergic neurons. In addition, the G-CSF dimer can significantly reduce apoptosis of neuron in hippocampus and improve learning and memory ability of AD model rats. Serum half-life of the G-CSF dimer of the invention is prolonged and the loss of neurons is effectively prevented, providing a better therapeutic effect in treatment of neurodegenerative disease.
    Type: Application
    Filed: July 24, 2012
    Publication date: September 4, 2014
    Applicant: GENERON SHANGHAI CORPORATION LTD.
    Inventors: Dong-Dong Wu, Zhihua Huang, Yuliang Huang, Xiaoqiang Yan
  • Publication number: 20140248334
    Abstract: The present invention concerns methods and compositions that employ peptides that target dorsal root ganglion (DRG) neurons. In particular, the peptides are used to target therapeutic agents, such as proteins, liposomes, or viral particles comprising therapeutic polynucleotides, to one or more peripheral neuropathies or neuropathic pain, for example. In particular cases, the peripheral neuropathies or neuropathic pain is caused directly or indirectly by DRG neuronopathy.
    Type: Application
    Filed: February 7, 2014
    Publication date: September 4, 2014
    Applicant: BAYLOR COLLEGE OF MEDICINE
    Inventors: Lawrence Chan, Hideto Kojima, Tomoya Terashima
  • Publication number: 20140243510
    Abstract: The present invention relates to a method for purifying an erythropoietin analog having a low isoelectric point below 4 by adding an N-linked sugar chain with high purity. In accordance with the present invention, the erythropoietin analog having an isoelectric point below 4, which is an isoform having more sialic acid residues, can be effectively purified via three-step chromatographic processes in short time at lower cost.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 28, 2014
    Applicant: CHONG KUN DANG PHARMACEUTICAL CORP.
    Inventors: Yeo-Wook Koh, Sang-Yong Lee, Cook-Hee Kim, Seung-Hui Lee, Ha-Na Kim, Su-Yon Kim, Jin-Hyun Seong, Yong-Hyun Cho
  • Patent number: 8815544
    Abstract: The present invention provides a novel lower eukaryotic host cell producing human-like glycoproteins characterized as having a terminal ?-galactose residue and essentially lacking fucose and sialic acid residues. The present invention also provides a method for catalyzing the transfer of a galactose residue from UDP-galactose onto an acceptor substrate in a recombinant lower eukaryotic host cell, which can be used as a therapeutic glycoprotein.
    Type: Grant
    Filed: July 21, 2010
    Date of Patent: August 26, 2014
    Assignee: Glycofi, Inc.
    Inventors: Robert Davidson, Tillman Gerngross, Stefan Wildt, Byung-Kwon Choi, Juergen Hermann Nett, Piotr Bobrowicz, Stephen Robin Hamilton
  • Patent number: 8809501
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Grant
    Filed: June 4, 2012
    Date of Patent: August 19, 2014
    Assignees: Baxter International Inc., Baxter Healthcare SA
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • Patent number: 8765924
    Abstract: This invention relates to novel protein conjugates, in particular, to novel pegylated proteins, and their methods of making and use. One aspect of the present invention relates to pegylated-erythropoietin having greater clinical efficacy and stability during shipment and storage than current erythropoietin formulations.
    Type: Grant
    Filed: July 16, 2007
    Date of Patent: July 1, 2014
    Assignee: Prolong Pharmaceuticals, Inc.
    Inventors: Abraham Abuchowski, Lihsyng Stanford Lee
  • Publication number: 20140162956
    Abstract: The present disclosure relates to a class of engineered polypeptides having a binding affinity for albumin. It also relates to new methods and uses that exploit binding by these and other compounds to albumin in different contexts, some of which have significance for treatment or diagnosis of disease in mammals including humans.
    Type: Application
    Filed: July 8, 2011
    Publication date: June 12, 2014
    Inventors: Caroline Ekblad, Lars Abrahmsen
  • Publication number: 20140163204
    Abstract: The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby.
    Type: Application
    Filed: November 25, 2013
    Publication date: June 12, 2014
    Applicant: Bolder Biotechnology, Inc.
    Inventor: George N. Cox
  • Publication number: 20140121159
    Abstract: The present disclosure provides inter alia conjugates of formula (I): wherein n, R1, R2, Rx, Z, X, Y and Z are as defined herein. A conjugate of formula (I) can also be converted to a conjugate of formulae (II) or (III) as described herein. Without limitation, the conjugates can be used to make controlled release materials and chemical sensors.
    Type: Application
    Filed: December 3, 2013
    Publication date: May 1, 2014
    Applicant: SmartCells, Inc.
    Inventors: Todd C. Zion, Thomas M. Lancaster
  • Publication number: 20140121351
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Application
    Filed: December 20, 2013
    Publication date: May 1, 2014
    Applicants: BAXTER HEALTHCARE SA, BAXTER INTERNATIONAL INC.
    Inventors: Stefan Haider, Andreas Ivens, Hanspeter Rottensteiner, Juregen Siekmann, Peter Turecek
  • Patent number: 8691767
    Abstract: Method for treatment and/or prophylaxis of schizophrenia and related psychoses of a human being, erythropoietin being administered to the human being.
    Type: Grant
    Filed: January 31, 2012
    Date of Patent: April 8, 2014
    Inventor: Hannelore Ehrenreich
  • Publication number: 20140073566
    Abstract: The present application discloses a method of treating a disease that is treatable by therapeutic angiogenesis comprising administering to a needy subject an effective amount of a chimeric coiled coil molecule comprising a coiled-coil domain linked to a receptor binding domain of a ligand.
    Type: Application
    Filed: March 20, 2013
    Publication date: March 13, 2014
    Applicant: Aprogen, Inc.
    Inventor: Aprogen, Inc.
  • Publication number: 20140058790
    Abstract: Methods and compositions described herein relate to processes for the production of deuterated peptides, and the deuterated peptides produced accordingly. Deuterated peptides produced according to methods and compositions described herein may be produced more efficiently than such peptides produced according to prior art processes. The production process of according to methods and compositions described herein may lead to advantages in yield, purity, and/or price for deuterated peptides. Methods of marketing deuterated peptides are also disclosed.
    Type: Application
    Filed: November 30, 2011
    Publication date: February 27, 2014
    Applicant: AmideBio LLC
    Inventors: Michael H.B. Stowell, Mikhail Plam
  • Publication number: 20140050693
    Abstract: Increased in vivo and/or in vitro stability is imparted to a biologically active protein by fusing to an amino acid sequence consisting of at least about 100 amino acid residues, which consist essentially of Alanine, Serine and Proline, which form a random coil conformation. Specific examples are described. Also described are related nucleic acids, vectors and cells encoding such amino acids; compositions of biologically active proteins fused to a random coil domain, and methods of making and using the compounds and compositions of the invention.
    Type: Application
    Filed: August 9, 2013
    Publication date: February 20, 2014
    Applicant: TECHNISCHE UNIVERSITAT MUNCHEN
    Inventors: Ame Skerra, Ina Theobald, Martin Schlapschy
  • Publication number: 20140045748
    Abstract: The present invention relates to a composition comprising erythropoietin (EPO) as an active ingredient for aiding surgical procedures for treating ischemic vascular diseases. The present invention also relates to a method for treating ischemic vascular diseases using said composition. The composition for aiding surgical procedures according to the present invention may improve the success rate of a variety of surgical procedures including multiple cutting operations performed on an adult patient suffering from a variety of ischemic vascular diseases including moyamoya disease, and can be widely used in the treatment of a variety of ischemic vascular diseases.
    Type: Application
    Filed: April 26, 2012
    Publication date: February 13, 2014
    Applicant: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventor: Ji Man Hong
  • Publication number: 20140039158
    Abstract: Methods of preparing polymer target conjugates which are substantially free of polymer attachment on the N-terminal of the targets are provided. Also provided are compositions comprising a plurality of polymer-polypeptide conjugates, said polymer-polypeptide conjugate comprising a polypeptide covalently attached to at least one polymer through an epsilon amino group of a Lysine or a Histidine found on the polypeptide and said conjugates containing less than 5% of the polymer-polypeptide conjugates having a polymer attached to the N-terminal of the polypeptide; and polymer target conjugates comprising a target moiety selected from the group consisting of polypeptides, proteins and the like having at least one polymer attached thereto at a site which is not the N-terminal of the target.
    Type: Application
    Filed: July 25, 2013
    Publication date: February 6, 2014
    Applicant: Belrose Pharma, Inc.
    Inventor: Hong Zhao
  • Patent number: 8642737
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Grant
    Filed: July 29, 2011
    Date of Patent: February 4, 2014
    Assignees: Baxter International Inc., Baxter Healthcare SA
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Andreas Ivens, Peter Turecek, Oliver Zoechling
  • Publication number: 20140031530
    Abstract: This invention provides long-acting, superactive analogs of glycoprotein hormones demonstrating enhanced bioactivity both in vitro and in vivo as compared to wild type counterparts. The analogs are particularly useful for treating subjects showing low receptor expression or poor receptor responsiveness, and for the treatment of any condition associated with glycoprotein hormone activity.
    Type: Application
    Filed: July 30, 2013
    Publication date: January 30, 2014
    Applicant: Trophogen Inc.
    Inventors: Mariusz Szkudlinski, Bruce D. Weintraub
  • Patent number: 8618266
    Abstract: The present invention relates to hydroxyalkylstarch (HAS)-polypeptide-conjugate (HAS-polypeptide) comprising one or more HAS molecules, wherein each HAS is conjugated to the polypeptide via a carbohydrate moiety or a thioether as well as to methods for the production thereof. In a preferred embodiment, the polypeptide is erythropoietin (EPO).
    Type: Grant
    Filed: March 11, 2005
    Date of Patent: December 31, 2013
    Assignee: Fresenius Kabi Deutschland GmbH
    Inventors: Harald S. Conradt, Eckart Grabenhorst, Manfred Nimtz, Norbert Zander, Ronald Frank, Wolfram Eichner
  • Publication number: 20130338067
    Abstract: The present invention provides fusion proteins comprising a mucin-domain polypeptide covalently linked to an active protein that has improved properties (e.g. pharmacokinetic and/or physicochemical properties) compared to the same active protein not linked to mucin-domain polypeptide, as well as methods for making and using the fusion proteins of the invention.
    Type: Application
    Filed: June 6, 2013
    Publication date: December 19, 2013
    Inventors: JUAN ALVAREZ, JEAN CHAMOUN, HEATHER C. LOSEY
  • Patent number: 8604175
    Abstract: The invention relates to a method for purifying a glycoprotein, preferably FSH or a FSH mutant, comprising the steps of subjecting a liquid containing FSH or a FSH mutant to: (1) a dye affinity chromatography; (2) a weak anion exchange chromatography; (3) a hydrophobic interaction chromatography; and (4) a strong anion exchange chromatography; which may be carried out in any order.
    Type: Grant
    Filed: December 6, 2006
    Date of Patent: December 10, 2013
    Assignee: Ares Trading S.A.
    Inventors: Thierry Ziegler, Mara Rossi, Antonello Datola, Sabrina Fiumi
  • Publication number: 20130323774
    Abstract: The present invention provides homogenous, fully-glycosylated, full length erythropoietin and the methods of producing the same.
    Type: Application
    Filed: April 30, 2013
    Publication date: December 5, 2013
    Applicant: SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH
    Inventors: Samuel J. Danishefsky, Ping Wang, Suwei Dong, Malcolm Andrew Stephen Moore, Jae-hung Shieh, John Andrew Brailsford
  • Publication number: 20130296252
    Abstract: Provided are MUC18 targeting peptides which may be used, e.g., to therapeutically target B-1 lymphocytes to reduce the influence of these cells on the metastatic potential of melanoma cells and/or to target cancerous cells, including certain melanoma and leukemia cells. MUC18 targeting peptides may be comprised in fusion constructs, imaging constructs, and/or therapeutic constructs such as fusion constructs which may be used for diagnosing or treating a cancer.
    Type: Application
    Filed: April 29, 2013
    Publication date: November 7, 2013
    Inventors: Fernanda STAQUICINI, Renata PASQUALINI, Wadih ARAP
  • Publication number: 20130261059
    Abstract: Compositions and methods for identifying patients at increased risk for PIGFD and ISS are disclosed.
    Type: Application
    Filed: May 26, 2011
    Publication date: October 3, 2013
    Applicant: THE CHILDREN'S HOSPITAL OF PHILADELPHIA
    Inventors: Yair Argon, Adda Grimberg
  • Publication number: 20130253176
    Abstract: Improved host cells and culture methods involving overexpression of MAN1C1 activity to improve protein production are provided.
    Type: Application
    Filed: May 30, 2013
    Publication date: September 26, 2013
    Applicant: Amgen Inc.
    Inventor: Christopher Kenyon Crowell
  • Patent number: 8541245
    Abstract: The present invention provides methods and kits for assessing the state of oocyte maturation in a female mammal based on the level of PAPP-A found in the female's bodily fluid sample.
    Type: Grant
    Filed: June 11, 2010
    Date of Patent: September 24, 2013
    Assignee: Beckman Coulter, Inc.
    Inventor: William E. Roudebush
  • Patent number: 8530191
    Abstract: A method for reducing or substantially preventing formation of a trisulfide derivative of a polypeptide in a liquid medium containing the polypeptide ijn question comprises stripping the liquid medium with a gas, suitably a chemically unreactive gas such as nitrogen or argon.
    Type: Grant
    Filed: March 1, 2010
    Date of Patent: September 10, 2013
    Assignee: Novo Nordisk A/S
    Inventors: Peter Becker, Thorkild Christensen