Abstract: A method is provided for producing an effective adjuvant response or stimulating the immune response of a warm blooded animal which comprises administering to said warm blooded animal an effective amount of a composition comprising refined detoxified endotoxin in combination with a pharmaceutically acceptable carrier.

Abstract: A fusion between DNA sequences coding for hAT and hGRF via a synthetic adaptor coding for an in vitro cleavable amino acid sequence is used to express hGRF at high levels in E. coli.

Abstract: Methods are disclosed for preparing (1) a pure, biologically active, immunogenic tumor-associated antigen by forming a soluble pool of membrane proteins from antigenic tissue and, by separating, identifying and characterizing antigenic and/or immunogenic proteins therefrom; (2) an ultrapure, biologically active TAA by further subjecting the pure TAA to isotachophoresis and/or affinity chromatography; and (3) making an epitope from purified TAA by injecting TAA into a host animal to stimulate the production by lymphocytes of antibodies specific to TAA, forming hybridomas capable of secreting monoclonal antibodies reactive with the TAA, dividing the TAA into fragments including epitopes, forming epitope-monoclonal antibody complexes, separating the complexes to recover the epitopes, and thereafter identifying and characterizing the epitopes.

Abstract: Vaccines containing both E.coli and herpes virus were found not to provide adequate protection against subsequent herpes virus infections. The present invention is concerned with a E.coli herpes virus combined vaccine which provides protection against both types of pathogen.

Abstract: The invention relates to a process and compounds useful therein for producing a compound having an amino acid sequence defining a biologically active peptide or protein from a compound of the formulaH--X--Pro--Peptidein which X is the residue of a naturally occurring amino acid and Peptide is a sequence of amino acids defining a biologically active peptide or protein, which comprises subjecting H--X--Pro--Peptide to conditions under which a diketopiperazine of the H--X--Pro-- moiety is formed with accompanying cleavage and release of Peptide.

Abstract: Methods for controlling the viscosity of protein hydrolysates by adjusting their magnesium and calcium content are disclosed. The viscosity may be reduced by adding a pyrophosphate or other precipitating agent to the hydrolysate and then removing the magnesium and/or calcium precipitate that is formed thereby. Alternatively, the viscosity may be increased by adding a magnesium and/or calcium containing source.

Abstract: Oligopeptide derivates corresponding to the general formulaMOOC--CHR.sup.1 --CHR.sup.2 --COHN--R.sup.3 (I)in which one of the groups R.sup.1 or R.sup.2 represents hydrogen or a C.sub.1 -C.sub.4 alkyl group while the other is a C.sub.6 -C.sub.22 alkyl or alkenyl group; R.sup.3 represents the residue of an oligopeptide which optionally contains other MOOC--CHR.sup.1 --CHR.sup.2 --CO-- groups attached to the nitrogen atom of basic amino acid side groups, and M represents hydrogen or an alkali, alkaline-earth, ammonium, mono-, di- or tri-alkanolammonium ion, are produced by partial hydrolysis of an animal or vegetable protein to a hydrolyzate having an average molecular weight of from 200 to 20,000 and reaction of the hydrolyzate with a substituted succinic acid anhydride corresponding to the following general formula ##STR1## in the presence of a base at a pH value above 8. These oligopeptide derivatives are suitable for use as surfactants which are gentle to the skin.

Abstract: A process for preparing nucleoproteic material which comprises immersing organic material into a suitable solvent for a sufficient time to extract nucleoproteins from said material, adding a sufficient amount of an acid to form a precipitate of nucleoproteic material, and recovering said nucleoproteic material precipitate. A composition of nucleoproteic material produced according to this process. A method for alleviating symptoms of neoplastic diseases which comprises sterilizing the composition of nucleoproteic material, preparing a formulation comprising an effective amount of said sterilized composition, and administering said formulation to a patient having symptoms of a neoplastic disease.

Abstract: Seeds of a grain legume of relatively low lipid content are processed under conditions found to yield an improved protein isolate which is well suited for human consumption. The seeds of the grain legume are wet-milled in an aqueous medium to form a finely milled slurry with the protein being substantially dissolved in water (as described). Solid material is removed from the protein slurry, and low molecular weight coagulation inhibitors inherently present are removed from the extract of protein by ultrafiltration with the aid of diafiltration to form a protein retentate. The protein retentate is coagulated through the action of proteolytic enzyme (as described) to produce the improved protein isolate of the present invention which optionally may be recovered in a particulate solid form. The resulting protein isolate of the present invention exhibits excellent emulsifying properties and can be used to advantage as an additive for food systems.

Abstract: Methods for controlling the viscosity of protein hydrolysates by adjusting their magnesium and calcium content are disclosed. The viscosity may be reduced by adding a pyrophosphate or other precipitating agent to the hydrolysate and then removing the magnesium and/or calcium precipitate that is formed thereby. Alternatively, the viscosity may be increased by adding a magnesium and/or calcium containing source.

Abstract: Dehydrated protein materials are treated with gaseous HCl without temperature control, the reaction temperature being susceptible to reach, momentarily, 150.degree. C. Then the material thus treated is degassed and, after drying, a non hygroscopic powder usable in the food industry or in the pharmaceutical industry is obtained.

Abstract: A pharmaceutical composition comprising a purified pyridine-soluble extract of a microorganism and a refined detoxified endotoxin which is effective in producing an immunological response in warm blooded animals and humans. Methods of using the composition for these purposes are also disclosed.

Abstract: New immunostimulant substances are obtained by hydrolyzing reduced and alkylated bovine fibrinogen with a proteolytic enzyme and fractionating the product.

Abstract: The present invention relates to a new class of novel Rhodamine derivatives. These derivatives which are bisamide substitution products are nonfluorescent. Whereas the monoamide substitution products exhibit high fluorescence. Cleavage of a single specified amide bond by bond-specific proteinases will therefore convert the nonfluorescent bisamide derivative into a highly fluorescent monoamide derivative.

Abstract: A method is provided for recovering useful products from de-oiled soymeal. At least two useful products, a soluble proteic substance and a mixture of free amino acids, are recovered.