Chromatography Or By Septum Selective As To Material, E.g., Gel Filtration, Molecular Sieve Dialysis, Etc. Patents (Class 530/417)
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Patent number: 8129507Abstract: An atopic dermatitis inducer binding to a human own IgE antibody and activating mast cells and basophiles, which includes a purified human secretion fraction, or an antigenic molecule or an antigenic determinant in the purified fraction, and obtained through the following steps of: filtering a human secretion, removing insoluble matters and collecting the filtrate; mixing the filtrate with a ConA-affinity carrier and collecting the supernatant; and separating a component having a histamine-releasing activity from the supernatant by column chromatography. This inducer is effective in diagnosing and treating human atopic dermatitis.Type: GrantFiled: August 10, 2009Date of Patent: March 6, 2012Assignee: Shionogi & Co., Ltd.Inventors: Michihiro Hide, Toshihiko Tanaka, Akio Tanaka, Kaori Ishii, Hidenori Suzuki
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Patent number: 8124377Abstract: The present invention relates to a new and nonobvious method of producing the C-terminal histidine tagged TAT-HOXB4 fusion protein (TAT-HOXB4H), providing unexpected benefits of increased yield and stability to allow for in vivo administration of this protein, and pharmaceutical composition comprising an effective ingredient, TAT-HOXB4H, having stimulatory activity on the production of hematopoietic cells. More specifically, recombinant TAT-HOXB4H protein enhances engraftment of bone marrow transplants, hematopoietic reconstruction, bone marrow re-population and number of circulating stem cells, particularly after chemotherapy or irradiation.Type: GrantFiled: October 18, 2010Date of Patent: February 28, 2012Assignee: Taiwan Advance Bio-Pharm Inc.Inventors: Kou-Juey Wu, Chi-Hung Huang
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HYDROPHOBIC MONOMERS, HYDROPHOBICALLY-DERIVATIZED SUPPORTS, AND METHODS OF MAKING AND USING THE SAME
Publication number: 20120039920Abstract: A composition is disclosed comprising a hydrophobic monomer having the structure: CH2?CR4C(O)NHC(R1R1)(C(R1R1))nC(O)XR3 wherein n is an integer of 0 or 1; R1 is independently selected from at least one of: a hydrogen atom, alkyls aryls, and alkylaryls, wherein the alkyls, aryls, and alkylaryls have a total of 10 carbon atoms or less; R3 is a hydrophobic group selected from at least one of: alkyls, aryls, alkylaryls and ethers, wherein the alkyls, aryls, alkylaryls and ethers have a total number of carbon atoms ranging from 4 to 30; R4 H or CH3; X is O or NH. In some embodiments the hydrophobic monomer is derived from an amine or an alcohol (HXR3) that has a hydrophilicity index of 25 or less. A polymerizable composition comprising the hydrophobic monomer is disclosed, which optionally may comprise a cross-linking monomer and/or a non-cross-linking monomer.Type: ApplicationFiled: March 19, 2010Publication date: February 16, 2012Inventors: Jerald K. Rasmussen, Cary A. Kipke, James I. Hembre, Peter D. Wickert -
Publication number: 20120029176Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).Type: ApplicationFiled: July 27, 2011Publication date: February 2, 2012Applicant: MILLIPORE CORPORATIONInventors: David Yavorsky, John Amara, Joaquin Umana, William Cataldo, Mikhail Kozlov, Matthew Stone
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Publication number: 20120022228Abstract: A process for purifying at least one product from a substrate containing at least one product, includes subjecting the substrate to centrifugal partition chromatography in an ion exchange displacement mode to purify the at least one product from the substrate, the at least one product being an amphoteric product.Type: ApplicationFiled: June 16, 2011Publication date: January 26, 2012Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, LONZA AG, UNIVERSITE DE REIMS CHAMPAGNE-ARDENNEInventors: Matthieu GIRAUD, John MCGARRITY, Jean-Hugues RENAULT, Leslie BOUDESOCQUE
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Publication number: 20120018380Abstract: A chromatography column that captures components in a process liquid in a free flow state and allows elution in steps is described.Type: ApplicationFiled: September 27, 2011Publication date: January 26, 2012Applicant: Therapeutic Proteins Inc.Inventor: Sarfaraz K. Niazi
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Publication number: 20120016113Abstract: A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification.Type: ApplicationFiled: September 26, 2011Publication date: January 19, 2012Applicant: Therapeutic Proteins IncInventor: Sarfaraz K. Niazi
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Publication number: 20120010390Abstract: The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase.Type: ApplicationFiled: January 7, 2010Publication date: January 12, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: James Van Alstine, Jamil Shanagar, Rolf Hjorth, Martin Hall, Camilla Estmer Nilsson
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Patent number: 8084224Abstract: A method to facilitate recovery troponin I and/or troponin T from a sample comprising addition of troponin C to the sample or to a surface from which the troponin I and/or troponin T are recovered.Type: GrantFiled: April 5, 2010Date of Patent: December 27, 2011Assignee: Alere San Diego, Inc.Inventors: Kenneth F. Buechler, Paul H. McPherson
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Patent number: 8080272Abstract: The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.Type: GrantFiled: January 9, 2008Date of Patent: December 20, 2011Assignee: Biova, L.L.C.Inventors: Ronald E. Strohbehn, Jesse I. Figgins
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Publication number: 20110301333Abstract: The present invention provides novel and improved compositions containing calcium phosphate and methods of using the same for the removal of protein aggregates from biopharmaceutical compositions containing a product of interest, e.g., a therapeutic antibody or protein.Type: ApplicationFiled: May 10, 2011Publication date: December 8, 2011Applicant: Millipore CorporationInventors: Ajish Potty, Alex Xenopolous
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Publication number: 20110301342Abstract: The invention is directed to an apparatus and method for purifying a protein. The apparatus involves the use of a capture chromatography resin, a depth filter arranged after the capture chromatography resin, and a mixed-mode chromatography resin arranged after the depth filter. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, a depth filter, and a mixed-mode chromatography resin. A membrane adsorber or monolith may be substituted for the mixed-mode chromatography column.Type: ApplicationFiled: April 13, 2011Publication date: December 8, 2011Applicant: ABBOTT LABORATORIES, INC.Inventors: Chen Wang, Robert K. Hickman, Edwin O. Lundell, Roy D. Hegedus
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Patent number: 8058014Abstract: The present invention is directed to methods of diagnosing a disease or predicting an increased risk of a disease, such as obesity, obesity-dependent subacute inflammation, atherosclerosis, cardiovascular disease and a metabolic disease, by determining the levels of omentin 1 and 2 protein in a subject, or by determining the levels of omentin 1 and 2 gene expression in a subject. The present invention is also directed to methods of disease treatment using omentin 1 protein and omentin 2 protein.Type: GrantFiled: September 29, 2007Date of Patent: November 15, 2011Assignee: University of Maryland, BaltimoreInventors: Da-Wei Gong, John C. McLenithan, Alan R. Shuldiner, Rong-Ze Yang
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Patent number: 8058411Abstract: The present invention relates to a method for producing a mature von Willebrand Factor (VWF) from von Willebrand Factor pro-peptide comprising the steps: immobilizing VWF pro-peptide on an ion exchange resin, incubating the immobilized VWF pro-peptide with furin to obtain immobilized mature VWF, and isolating mature VWF from the ion exchange resin by elution.Type: GrantFiled: May 16, 2008Date of Patent: November 15, 2011Assignees: Baxter International Inc., Baxter Healthcare S.A.Inventors: Wolfgang Mundt, Artur Mitterer, Meinhard Hasslacher, Christa Mayer
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Publication number: 20110263834Abstract: This invention is directed to methods for removing, preferably simultaneously and in one step, multiple impurities form crude culture samples, and, in particular, the removal of media components, protein, nucleic acids, lipids, and lipopolysaccharides to ultralow levels. Preferably the purification process comprises: (1) binding of the target substance containing one or more contaminants to a chromatography matrix; (2) washing the bound target substance with one or more buffers containing a synergistic combination of a lyotropic agent or organic solvent, a detergent, and a salt component; and (3) desorbing the target substance from the chromatography matrix, so that the eluate contains ultra low levels of contaminants. The reduction of impurities that can be achieved is preferably 91-99.9% as compared to the amount of impurities in the target substance before purification. The invention is also directed to the targets products that have been so purified.Type: ApplicationFiled: December 20, 2010Publication date: October 27, 2011Applicants: FinaBioSolutions, LLC, Serum Institute of India, Ltd.Inventors: Andrew Lees, Jayant Sakaram Joshi
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Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol
Patent number: 7998705Abstract: The present invention generally relates to novel processes for protein purification in high salt solutions such as cell culture broth by increasing the dynamic binding capacity of a resin with the addition of polyethylene glycol.Type: GrantFiled: November 12, 2002Date of Patent: August 16, 2011Assignee: FUJIFILM Diosynth Biotechnologies U.S.A., IncInventors: Min Wan, Mark David Chavez, Jeffrey Lee Schrimsher -
Publication number: 20110150911Abstract: Immunogenic compositions for use in treating, preventing and diagnosing infection caused by the California (CAL) serotype of the genus Bunyavirus, such as La Crosse virus (LACV), are disclosed. Also described are reagents for use in diagnostic assays.Type: ApplicationFiled: November 19, 2004Publication date: June 23, 2011Inventors: Qui-Lim Choo, Michael Houghton, Elizabeth Scott, Amy Weiner
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Patent number: 7959926Abstract: The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH comprising non-naturally encoded amino acids and hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules.Type: GrantFiled: October 17, 2007Date of Patent: June 14, 2011Assignee: Ambrx, Inc.Inventors: Ying Buechler, Ricky Lieu, Michael Ong, Stuart Bussell, Nick Knudsen, Ho Sung Cho
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Publication number: 20110120947Abstract: There is provided a crosslinked cellulose hydrate membrane having a porous double structure which consists of micropores having a diameter in the range from >100 nm to 20 ?m and ultrapores which have a diameter of <100 nm and which are not accessible to Blue Dextran having an average molecular weight Mw of 2 000 000, wherein the fraction of the volume of the ultrapores is more than 15% of the entire pore volume accessible to water, and wherein hydrophobic ligands, selected from C1-C20-alkyl and their derivatives or C6-C25-aryl and their derivatives or C7-C25-arylalkyl and their derivatives or —[(CH2)m—O—]n—R, where m is 2 or 3, n is a whole number greater than or equal to 1, and R is —H or —C1-C5-alkyl, are bonded to the membrane. In addition, methods for producing the membrane, an apparatus for hydrophobic interaction chromatography and comprising the membrane, and the use of the membrane in hydrophobic interaction chromatography are specified.Type: ApplicationFiled: February 10, 2009Publication date: May 26, 2011Applicant: SARTORIUS STEDIM BIOTECH GMBHInventor: René Faber
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Patent number: 7943046Abstract: Embodiments of the present invention provide a method of chromatographic delipidation comprising separating a lipid-containing sample on a superficially porous stationary phase at greater than about 70° C., at least about 80° C., having at least one mobile phase comprising an ion-pairing agent in water, an ion-pairing agent in an organic modifier, an acid in an organic modifier, and an alcohol. The invention provides minimal protein losses and high run-to-run reproducibility. The on-column delipidation method aventageously utilize reversed phase liquid chromatography.Type: GrantFiled: June 22, 2006Date of Patent: May 17, 2011Assignee: Agilent Technologies, IncInventors: James D. Martosella, Nina I. Zolotarjova
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Patent number: 7928191Abstract: A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.Type: GrantFiled: August 29, 2008Date of Patent: April 19, 2011Assignee: Fujifilm CorporationInventors: Koichi Minami, Yohsuke Takeuchi, Taisei Nishimi
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Publication number: 20110082283Abstract: ELP fusion proteins, multimeric ELP spider complexes formed of ELP fusion proteins, and methods of using the same. The construct may be in the form of an ELP spider structure complex including multi-leg moieties comprising ELP fusion proteins capable of forming covalent disulfide bonds. The multimeric fusion constructs may be employed in peptide production and purification and/or to enhance proteolytic resistance of a protein or peptide moiety in a fusion construct, by provision of the fusion protein in an ELP spider complex.Type: ApplicationFiled: February 25, 2010Publication date: April 7, 2011Inventor: Suzanne DAGHER
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Publication number: 20110065897Abstract: The present invention relates to a method for isolating and/or purifying at least one polypeptide from a polypeptide-containing sample, characterized in that the sample is contacted with a boron carbide support material at a pH which allows the binding of the polypeptide to the boron carbide support material. Such isolating can, for example, be used to remove polypeptides from a sample or else to purify and/or to concentrate polypeptides. A matrix comprising a boron carbide support material for purification of polypeptides is further disclosed according to the invention.Type: ApplicationFiled: April 9, 2009Publication date: March 17, 2011Inventor: Christian Feckler
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Patent number: 7901911Abstract: The present invention intends to provide an IL-6R.IL-6 fusion protein and the like in which IL-6R and IL-6 are directly linked without a linker. The IL-6 receptor.IL-6 fusion protein of the present invention has a structure in which one amino acid residue constituting IL-6 receptor and one amino acid residue constituting IL-6 are directly bonded.Type: GrantFiled: March 19, 2009Date of Patent: March 8, 2011Assignee: Tosoh CorporationInventors: Teiji Ekida, Harutaka Yagame, Hiroshi Iida, Kiyoshi Yasukawa, Shigeo Tsuchiya, Teruhiko Ide
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Patent number: 7858341Abstract: The expression vectors and methods using an E. coli expression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the FGF18 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains, as well as OmpT and fhuA negative strains transformed with an FGF18 expression vector.Type: GrantFiled: December 12, 2005Date of Patent: December 28, 2010Assignee: ZymoGenetics, Inc.Inventors: Brian J. Reardon, Susan H. Julien, Chung-leung Chan, Hong Y. Liu
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METHOD FOR MANUFACTURING MESOPOROUS MATERIALS, MATERIALS SO PRODUCED AND USE OF MESOPOROUS MATERIALS
Publication number: 20100310539Abstract: The present invention relates to a new synthetise for the preparation of mesoporous structures including mesoporous materials with chiral morphologies and mesoporous materials with local or surface chirality. The method can be used for manufacturing controlled drug delivery devices, for example for delivery of folic acid, and fluorescent particles.Type: ApplicationFiled: February 11, 2009Publication date: December 9, 2010Inventor: Alfronso Garcia-Bennett -
Publication number: 20100311953Abstract: The invention relates to a process for purifying a protein by mixing a protein preparation with a solution having a first salt and a second salt, wherein each salt has a different lyotropic value, and loading the mixture onto a hydrophobic interaction chromatography column. The dynamic capacity of the column for a protein using the two salt combination will be increased compared with the dynamic capacity of the column for either single salt alone.Type: ApplicationFiled: June 23, 2010Publication date: December 9, 2010Applicant: Amgen Inc.Inventors: Anna Senczuk, Ralph Klinke
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Publication number: 20100286071Abstract: Members of the TGF-? superfamily and peptide fragments based on member proteins are employed to purify solutions containing member proteins or as therapeutics.Type: ApplicationFiled: June 17, 2010Publication date: November 11, 2010Applicant: Wyeth LLCInventors: Zhijian Lu, Wei Liu, Jimin Zhang, Paul John Yaworsky, Stephane H. Olland, Christopher Todd Brown, Emily Sheng-ming Shen
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Patent number: 7820800Abstract: The invention relates to a process for the purification of IL-18 binding protein (IL-18BP) from a fluid comprising hydrophobic charge-induction chromatography.Type: GrantFiled: November 4, 2004Date of Patent: October 26, 2010Assignee: Ares Trading S.A.Inventors: Mara Rossi, Thierry Ziegler, Laure Valognes
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Patent number: 7816495Abstract: The present invention relates to processes for the purification of fibrinogen, and to readily solubilised fibrinogen preparations.Type: GrantFiled: July 7, 2003Date of Patent: October 19, 2010Assignee: NHS Blood and TransplantInventors: Sarah Kingsland, Robert Clemmitt, David Evans, Peter Feldman
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Publication number: 20100261886Abstract: The invention provides methods for isolating proteins in purified form from mixtures by precipitation with citrate. The methods are advantageous in that they effectively separate a protein from lower molecular weight contaminants, including fragments or portions of the protein. Such methods are particularly useful for purifying antibodies from mixtures containing antibody proteolytic fragments and unpaired chains.Type: ApplicationFiled: April 13, 2010Publication date: October 14, 2010Applicant: Bristol-Myers Squibb CompanyInventors: Alahari Arunakumari, Gisela M. Ferreira
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Patent number: 7795405Abstract: After the sequencing of the human genome, great interest has developed in trying to discern the complementary proteome of humans and other species. The present disclosure provides devices, systems, and methods for proteomic fractionation that may increase the number of protein spots visualized by 2DE analysis, and may allow enrichment of proteins normally not detectable by standard 2DE analysis. According to some embodiments of the disclosure, devices, systems, and methods of the disclosure relate to fractionating a proteome on the basis of surface charge, hydrophobicity, isoelectric point and/or size.Type: GrantFiled: January 29, 2007Date of Patent: September 14, 2010Assignee: Guild Associates, Inc.Inventor: Augustine DiNovo
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Patent number: 7771725Abstract: The invention concerns a new medicinal product for the treatment of arboviruses, i.e.. a concentrate of immunoglobulins and F(ab)?2 and/or Fab fragments specific to said arbovirus as well as its process of preparation.Type: GrantFiled: April 2, 2007Date of Patent: August 10, 2010Assignee: Laboratoire Fancais du Fractionnement et des BiotechnologiesInventor: Roland Schmitthaeusler
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Patent number: 7756646Abstract: A method of predicting whether a peptide present in a biological sample will be detected by analysis with a mass spectrometer. The method uses at least one mass spectrometer to perform repeated analysis of a sample containing peptides from proteins with known amino acids. The method then generates a data set of peptides identified as contained within the sample by the repeated analysis. The method then calculates the probability that a specific peptide in the data set was detected in the repeated analysis. The method then creates a plurality of vectors, where each vector has a plurality of dimensions, and each dimension represents a property of one or more of the amino acids present in each peptide and adjacent peptides in the data set. Using these vectors, the method then generates an algorithm from the plurality of vectors and the calculated probabilities that specific peptides in the data set were detected in the repeated analysis.Type: GrantFiled: March 31, 2006Date of Patent: July 13, 2010Assignee: Battelle Memorial InstituteInventors: Lars Kangas, Richard D. Smith, Konstantinos Petritis
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Patent number: 7754689Abstract: Members of the TGF-? superfamily and peptide fragments based on member proteins are employed to purify solutions containing member proteins or as therapeutics.Type: GrantFiled: June 1, 2007Date of Patent: July 13, 2010Assignee: Wyeth LLCInventors: Zhijian Lu, Wei Liu, Jimin Zhang, Paul John Yaworsky, Stephane H. Olland, Christopher Todd Brown, Emily Sheng-ming Shen
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Patent number: 7754860Abstract: The invention provides a method for purifying recombinant FSH.Type: GrantFiled: December 16, 2004Date of Patent: July 13, 2010Assignee: Ares Trading S.A.Inventor: Mara Rossi
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Patent number: 7744890Abstract: Methods for reducing the opalescent appearance of a protein preparation by modifying the ionic strength of the preparation, as well as compositions, e.g., pharmaceutical compositions, of concentrated protein with decreased opalescence are disclosed. Purification methods which monitor and/or reduce the salt concentrations at selected steps are also disclosed.Type: GrantFiled: October 12, 2007Date of Patent: June 29, 2010Assignee: Wyeth LLCInventors: Pilarin Elizabeth Nichols, Donna L. Luisi
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Patent number: 7745582Abstract: The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matrices, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.Type: GrantFiled: October 31, 2007Date of Patent: June 29, 2010Assignee: Upfront ChromatographyInventors: Allan Otto Fog Lihme, Marie Bendix Hansen
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Publication number: 20100160605Abstract: To provide a novel packing material for liquid chromatography capable of separating and purifying, or collecting and recovering, a biopolymer such as a protein or a peptide by adsorption and desorption by a pH change without being influenced by the isoelectric point of the protein or by the salt concentration in a solvent in which the biopolymer such as the protein is dissolved, and to provide a process for concentrating and recovering a desired biopolymer such as a protein or a peptide from a large amount of dilute cell culture solution by means of such a packing material.Type: ApplicationFiled: December 17, 2009Publication date: June 24, 2010Applicant: TOSOH CORPORATIONInventors: Katsuo KOMIYA, Koji NAKAMURA
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Publication number: 20100151581Abstract: The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.Type: ApplicationFiled: February 22, 2010Publication date: June 17, 2010Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: GUNNAR GLAD, BO-LENNART JOHANSSON, JEAN-LUC MALOISEL, NILS NORRMAN
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Publication number: 20100144622Abstract: Methods of producing properly refolded recombinant plasminogen and plasmin polypeptide are provided. Denatured recombinant plasminogen polypeptide is refolded by first solubilizing the polypeptide with a chaotroph and reducing and oxidizing agents at high pH, followed by refolding in the presence of reduced concentration of chaotroph and reducing and oxidizing agents and in the presence of arginine.Type: ApplicationFiled: September 28, 2007Publication date: June 10, 2010Inventors: Xinli Lin, Daniel Medynski
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Patent number: 7728116Abstract: In a method of isolating osteogenic protein from bone, in which an osteogenic protein-containing fraction is extracted from bone and enriched by a sequence of enrichment steps selected from ultrafiltration and chromatography, the invention provides the improvement of removing higher molecular weight components from the osteogenic protein-containing fraction prior to the enrichment steps. The higher molecular weight components have a molecular weight of about 100-300 kDa and are selected from collagen, collagen fragments, collagen aggregates and mixtures thereof.Type: GrantFiled: June 18, 2003Date of Patent: June 1, 2010Assignee: Altis Biologics (Proprietary) LimitedInventor: Nicolaas Duneas
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Patent number: 7723492Abstract: Uses of recombinant procalcitonin 3-116 in the diagnosis and therapy of septic diseases and the measurement of prohormones other than procalcitonin, and of dipeptidyl peptidase IV, as biomarkers in the diagnosis of sepsis.Type: GrantFiled: September 29, 2008Date of Patent: May 25, 2010Assignee: B.R.A.H.M.S. AktiengesellschaftInventors: Andreas Bergmann, Joachim Struck, Wolfgang Weglöhner
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Patent number: 7718845Abstract: The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos.Type: GrantFiled: September 29, 2004Date of Patent: May 18, 2010Assignee: Sterrenbeld Biotechnologie North America, Inc.Inventors: Carlos Alberto Melo, Lino Baranao, Cesar Horacio Carbonetto
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Publication number: 20100113751Abstract: The present invention provides a method of removing product-related inactive or partially active species, high molecular weight aggregates, as well as other process-related impurities from preparations of acidic proteins by using ceramic hydroxyapatite chromatography.Type: ApplicationFiled: October 29, 2009Publication date: May 6, 2010Applicant: WyethInventors: Shujun Sun, Yin Luo, Priscilla Jennings
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Publication number: 20100105871Abstract: The invention relates to a process for the removal of glycoalkaloids, in particular from process streams such as those encountered during isolation of proteins from potatoes.Type: ApplicationFiled: October 25, 2007Publication date: April 29, 2010Inventors: Marco Luigi Federico Giuseppin, Robin Eric Jacobus Spelbrink
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Patent number: 7695973Abstract: The present invention provides methods for quantitation of glycated protein in a biological sample using a solid support matrix by making a first bound protein measurement total bound protein under conditions where both glycated and non-glycated protein bind to the support in making a second bound protein measurement under conditions where glycated protein is bound to the support and non-glycated protein is not substantially bound. Diagnostic devices and kits comprising the methods of the present invention are also provided.Type: GrantFiled: February 7, 2007Date of Patent: April 13, 2010Assignee: Scripps Laboratories, Inc.Inventors: Ralph P. McCroskey, Cameron E. Melton
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RECOMBINANT VITAMIN K DEPENDENT PROTEINS WITH HIGH SIALIC ACID CONTENT AND METHODS OF PREPARING SAME
Publication number: 20100081187Abstract: Methods of isolating highly sialylated recombinant vitamin K dependent proteins, particularly Factor IX, by chromatographic methods are described. The highly sialylated recombinant proteins are characterized. The improved Factor IX has at least 62% N-glycosylation with 3 or 4 sialic acid residues and improved bioavailability and pharmokinetic properties.Type: ApplicationFiled: April 28, 2008Publication date: April 1, 2010Inventors: Michael J. Griffith, William N. Drohan, Marian J. Drohan -
Patent number: RE41555Abstract: This invention relates to the application of hydrophobic interaction chromatography combination chromatography to the purification of antibody molecular proteins.Type: GrantFiled: May 18, 2007Date of Patent: August 24, 2010Assignee: Glaxosmithkline LLCInventors: Paula Shadle, John C. Erickson, Robert G. Scott, Thomas M. Smith
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Patent number: RE41595Abstract: This invention relates to the application of hydrophobic interaction chromatography combination chromatography to the purification of antibody molecule proteins.Type: GrantFiled: March 13, 2009Date of Patent: August 31, 2010Assignee: GlaxoSmithKline LLCInventors: Paul J. Shandle, John C. Erickson, Robert G. Scott, Thomas M. Smith