Abstract: A novel leaching process for separating extractable material from a particulate solid material that comprises extractable and non-extractable portions includes the steps of introducing the particulate solid material and a liquid solvent to an extraction zone; agitating the liquid solvent to a degree sufficient to suspend the particulate solid material and effect segregation of particles thereof in relation to their propensity to settle; maintaining contact between the liquid solvent and the particulate solid material for a time sufficient to leach extractable material therefrom; and selectively withdrawing particles of the solid material from the extraction zone. In a preferred embodiment, the leaching process is a multi-stage process in which the selectively withdrawn particles from each stage are introduced to the next successive stage of the series.

Abstract: A method for the recovery of proteins which method includes providing a source of protein in an insoluble form, a source of at least one cationic surfactant; treating the insoluble protein with the at least one cationic surfactant in an amount sufficient to effect solubilization without substantial modification to the structural backbone of the protein.

Abstract: The invention relates to an immunogenic complex containing antigenic membrane proteins or peptides from viruses mycoplasmas, bacteria, parasites or animal cells or prepared synthetically or with hybride DNA technique and glycoside. The invention also relates to a process for producing the complex, whereby microorganisms, animal cells, proteins and peptides are mixed with solubilizing agents in buffered, possibly saline, solution, whereby complexes are formed between charged monomeric antigenic proteins and detergent or peptides and solubilizing agents, whereafter the charged monomeric antigenic proteins or peptides are separated from solubilizing agents in the presence of, or are separated from solubilizing agent and directly transferred to, a glycoside solution which contains one or more glycosides with hydrophobic and hydrophilic regions in a concentration of at least the critical micellular concentration, whereby a protein complex is formed, which is isolated and purified.

Abstract: Lewis acid-Lewis base high molecular weight salt microparticulate material of the type referred to in U.S. Pat. No. 3,959,457, which include biologically active peptides and proteins solubilized in a non-aqueous, non-denaturing manufacturing solvent.

Abstract: A method for extracting protein produced by procaryotic or eucaryotic cells comprising contacting said cells with a solution containing from about 50 to about 100 volume percent of an organic acid having from 1 to 5 carbon atoms and mixtures thereof is disclosed.

Abstract: A method is provided for forming ordered macromolecular protein arrays by avoiding a binding pathway that leads to densely packed, disordered states during protein crystal growth, by a diffusion limited process or by allowing controlled growth to occur by removal of the initial supported protein layer to a different environment.

Abstract: This invention discloses a novel process for the solubilization, purification and characterization of a protein or proteins from insoluble protein aggregates or complexes. The novel process comprises the use of a dissociating step gradient which can be followed by further purification and concentration. Also disclosed are compositions of matter and vaccines comprising one or more proteins purified according to the novel process of this invention.