Probes For Detection Of Microbial Nucleotide Sequences Patents (Class 536/24.32)
  • Patent number: 10400244
    Abstract: The present invention comprises a novel artificial oligonucleotide sequence which can initiate the transcription of a gene under various conditions at a high level. Further the invention relates to a recombinant DNA fragment comprising the artificial oligonucleotide sequence, an expression plasmid comprising the recombinant DNA fragment and a host cell transformed with the recombinant DNA fragment.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: September 3, 2019
    Assignee: Clariant International Ltd.
    Inventors: Zdravko Dragovic, Christoph Reisinger, Heiko Dietz
  • Patent number: 9987298
    Abstract: Provided by the present invention are an aptamer having an inhibitory activity on FGF2; a complex containing an aptamer having a binding activity or an inhibitory activity on FGF2, and a functional substance (e.g., affinity substance, labeling substance, enzyme, drug delivery vehicle, or drug and the like); a medicament, diagnostic reagent or labeling agent containing an aptamer having a binding activity or an inhibitory activity on FGF2, or a complex containing said aptamer and a functional substance; and the like.
    Type: Grant
    Filed: March 24, 2015
    Date of Patent: June 5, 2018
    Assignee: RIBOMIC INC.
    Inventor: Ling Jin
  • Patent number: 9885092
    Abstract: Provided are nucleic acids capable of hybridizing to HPV 16 and/or HPV 18 nucleic acids, in particular, mRNA encoding E2 and E6-7 gene products. Such nucleic acids are useful in methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, methods and means for predicting the progression of precancerous cervical lesions, and methods and means for detecting disruption of genes or gene expression.
    Type: Grant
    Filed: February 23, 2012
    Date of Patent: February 6, 2018
    Assignee: QIAGEN GAITHERSBURG INC.
    Inventors: Brian Lowe, Anna K. Fulbright, Irina Nazarenko
  • Patent number: 9499848
    Abstract: In a method for generating a long nucleic acid molecule, nucleic acids immobilized on a surface and having overlapping complementary sequences is released into solution. The overlapping complementary sequences are hybridized to form hybridized nucleic acids, followed by extension or ligation of the hybridized nucleic acids to synthesize the long nucleic acid molecule. The nucleic acids may comprise first and second series of nucleic acids having redundant overlapping sequences, wherein nucleic acids from the first and second series are complementary to each other. The complementary nucleic acids are hybridized to form the hybridized nucleic acids. The generated long nucleic acid molecule may have a predetermined sequence element, and it may be introduced into a system wherein the predetermined sequence element is required for replication, such that replication of the synthesized long nucleic acid molecule is indicative of the presence of the predetermined sequence element in the long nucleic acid molecule.
    Type: Grant
    Filed: August 13, 2013
    Date of Patent: November 22, 2016
    Assignee: Massachusetts Institute of Technology
    Inventors: Peter A. Carr, Brian Y. Chow, Joseph M. Jacobson, David W. Mosley, Christopher Emig
  • Patent number: 9434986
    Abstract: The invention encompasses method of using quantitative PCR to detect fungal organisms in clinical and environmental samples to generate standards that allow the quantification of fungal organisms in the samples.
    Type: Grant
    Filed: May 12, 2014
    Date of Patent: September 6, 2016
    Assignees: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE, ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY
    Inventors: Cindy Liu, Sergey Kachur, Lance Price, Paul Keim
  • Patent number: 9371534
    Abstract: Provided is a method for producing an expression product of an exogenous gene in a yeast with superior output regulation of gene expression. This is a method for producing an expression product of an exogenous gene in a yeast, including using one or two or more kinds of yeast gene terminator regions selected based on expression intensity data related to the expression intensity of yeast gene terminator regions to cause expression of a gene in a yeast.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: June 21, 2016
    Assignee: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO
    Inventors: Takashi Matsuyama, Mamoru Yamanishi, Chie Imamura, Satoshi Katahira, Akinori Ikeuchi, Yoichiro Ito
  • Patent number: 9347938
    Abstract: Compositions, methods, and devices for the detection of multiple analytes with a single signal are provided.
    Type: Grant
    Filed: March 7, 2013
    Date of Patent: May 24, 2016
    Assignee: Invisible Sentinel, Inc.
    Inventors: Nicholas Siciliano, Louis Leong, Martin Patrick Keough, Ashley Shaniece Brown
  • Patent number: 9175353
    Abstract: The disclosed invention is related to compositions, kits and methods comprising one or more oligomers targeting 16S rRNA target nucleic acid from Campylobacter species jejuni, coli and/or lari. Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.
    Type: Grant
    Filed: November 16, 2009
    Date of Patent: November 3, 2015
    Assignee: Gen-Probe Incorporated
    Inventors: Michael R. Reshatoff, Jr., Jennifer J. Bungo, Shannon K. Kaplan, James J. Hogan
  • Publication number: 20150132260
    Abstract: The present invention relates to the use of the measure of anelloviral load for the determination of immunosuppression. More precisely, the present invention provides a method for characterizing the immunosuppressed or non-immunosuppressed status of a subject, comprising the steps of determining the anelloviral load from a biological sample of the said subject, and determining from the said comparison the immunosuppressed or non-immunosuppressed status. The determination of the immunosuppressed status of the subject can then be used to design or adapt a therapeutic treatment.
    Type: Application
    Filed: April 19, 2013
    Publication date: May 14, 2015
    Inventors: Marc Eloit, Justine Cheval, Charles Hebert, Marc Lecuit
  • Publication number: 20150126435
    Abstract: Disclosed are primers, probes, and single nucleotide polymorphisms (SNP) specific to a distinct subclones of the E. coli sequence type 131 (ST131). Also disclosed are methods and assay kits useful in detecting the presence of the distinct subtype of E. coli and methods of treating a subject suffering from an infection from a subclone of ST131.
    Type: Application
    Filed: July 2, 2013
    Publication date: May 7, 2015
    Inventors: Lance B. Price, Evgueni V. Sokurenko, James R. Johnson
  • Publication number: 20150119277
    Abstract: The invention discloses an in vitro method for the identification of Candida tropicalis, the sequences associated to said identification, as well as diagnosis kits for identifying Candida tropicalis.
    Type: Application
    Filed: October 21, 2014
    Publication date: April 30, 2015
    Inventors: Irene Beatriz CASTAÑO NAVARRO, Alejandro DE LAS PEÑAS NAVA
  • Publication number: 20150111207
    Abstract: Sequences having specificity for Mycoplasma and related Mollicutes genus strains and uses thereof. Methods of use include detection of samples contaminated with Mycoplasma. Kits are provided and comprise one or more oligonucleotides for the detection of Mycoplasma and related Mollicutes genus strains.
    Type: Application
    Filed: May 31, 2013
    Publication date: April 23, 2015
    Inventors: Claudia Zylberberg, Juan Manuel Rodriguez, Andres Clemente Hernando Insua, Ricardo Ariel Lopez
  • Publication number: 20150111206
    Abstract: The invention relates to a method for specifically detecting and optionally for quantifying Mycobacterium avium ssp. paratuberculosis (MAP) in a sample of an individual. According to the method according to the invention, for this purpose the detection of the presence of the IS900 region in a sample is performed by means a nucleic acid amplification and specific oligonucleotides. In a further aspect, the invention relates to a test kit for specifically detecting MAP in a sample by means of amplification methods. Finally, the invention relates to specific oligonucleotides that are suitable for specifically detecting MAP.
    Type: Application
    Filed: April 26, 2013
    Publication date: April 23, 2015
    Inventors: Claus-Peter Czerny, Pia Muenster
  • Publication number: 20150105282
    Abstract: The invention concerns a screening method for the detection of Clostridium difficile in a sample, wherein said method comprises the detection of conserved target regions in the Clostridium genome through the binding of at least one oligonucleotide probe to said target site.
    Type: Application
    Filed: May 2, 2013
    Publication date: April 16, 2015
    Inventors: Lesley William James Baillie, Lovleen Tina Joshi
  • Publication number: 20150099265
    Abstract: Improved dipstick assays for testing for the presence of a target nucleic acid in a sample solution are described. A dipstick is provided which comprises a contact end for contacting the sample solution and a capture zone remote from the contact end for capturing target nucleic acid. Sample solution is contacted with the contact end to cause sample solution to move by capillary action to the capture zone. Target nucleic acid in the sample solution is captured at the capture zone and is detected by a plurality of different labelled detection probes each capable of hybridizing to a different region of the target nucleic acid. The detection signal is thereby enhanced. In other methods a plurality of different capture probes are added to the sample solution which can then be bound by a capture moiety at the capture zone to indirectly capture target nucleic acid. Capture of target nucleic acid is thereby improved. Kits and dipsticks for carrying out such methods are also described.
    Type: Application
    Filed: December 19, 2013
    Publication date: April 9, 2015
    Applicant: Diagnostics for the Real World, Ltd.
    Inventors: Helen Lee, Magda Anastassova Dineva
  • Publication number: 20150093748
    Abstract: The present invention provides a method for detecting the presence of microcystin-producing toxic cyanobacteria in a sample comprising the step of contacting nucleic acid obtained from an environmental sample with primers specifically hybridizing with the nucleic acid sequence of mcy B gene present in Microcystis aeruginosa, Anabaena sp. and Planktothrix agardhii. The present invention further provides primers, primer pairs and probes designed for the method of the invention.
    Type: Application
    Filed: November 9, 2012
    Publication date: April 2, 2015
    Inventors: Henna Savela, Markus Vehniäinen
  • Publication number: 20150086577
    Abstract: Described herein are the clinical and laboratory characteristics of two patients bitten by ticks and infected with a unique member of the genus Phlebovirus (family Bunyaviridae) with a proposed name of Heartland virus (HRTLV). Provided herein are nucleotide and amino acid sequences of the Phlebovirus isolates, primers and probes that specifically hybridize with the Phlebovirus isolates, and antibodies specific for the Phlebovirus proteins. Also provided are detection assays using the Phlebovirus nucleic acid molecules, proteins, probes, primers and antibodies. Further provided are recombinant Phleboviruses and their use for eliciting an immune response in a subject.
    Type: Application
    Filed: March 22, 2013
    Publication date: March 26, 2015
    Applicant: The Government of the United States of America as represented by the Secretary of the Department of
    Inventors: Laura K. McMullan, Cynthia Goldsmith, Aubree Kelly, William L. Nicholson, Stuart T. Nichol
  • Publication number: 20150086987
    Abstract: Disclosed herein are methods for detecting presence of one or more of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Toxoplasma gondii, Moraxella catarrhalis, Escherichia coli, Shigella, Staphylococcus aureus, Pneumocystis jirovecii, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Ureaplasma spp., Bartonella spp., Streptococcus agalactiae, and Neisseria meningitidis nucleic acids in a sample, such as a biological sample obtained from a subject, or an environmental sample. This disclosure also provides probes, primers, and kits for detecting one or more of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Toxoplasma gondii, Moraxella catarrhalis, Escherichia coli, Shigella, Staphylococcus aureus, Pneumocystis jirovecii, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Ureaplasma spp., Bartonella spp., Streptococcus agalactiae, and Neisseria meningitidis in a sample.
    Type: Application
    Filed: February 27, 2013
    Publication date: March 26, 2015
    Inventors: Bernard Wolff, Jonas M. Winchell, Maureen Diaz
  • Publication number: 20150079209
    Abstract: The present invention provides a diabetes-inducing bacterium and use thereof, and a reagent and a detection method for detecting the bacterium. According to the present invention, enteric colonization of the diabetes-inducing bacterium can be prevented, and diabetes can be treated and/or prevented.
    Type: Application
    Filed: September 26, 2014
    Publication date: March 19, 2015
    Applicant: AJINOMOTO CO., INC.
    Inventors: Keishi KAMEYAMA, Kikuji Itoh
  • Publication number: 20150079589
    Abstract: This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistant Mycobacterium tuberculosis in a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistant M. tuberculosis in a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wildtype RRDR indicates the presence of rifampicin resistant M. tuberculosis in the sample.
    Type: Application
    Filed: April 10, 2013
    Publication date: March 19, 2015
    Applicant: Rutgers, The State University of New Jersey
    Inventors: David Alland, Soumitesh Chakravorty
  • Patent number: 8980562
    Abstract: The invention relates to a method of detecting HPV and determining HPV type.
    Type: Grant
    Filed: June 12, 2008
    Date of Patent: March 17, 2015
    Assignee: Physicians Reference Laboratory
    Inventor: Pradip Manna
  • Patent number: 8980545
    Abstract: Presented herein is the discovery of a new human picornavirus, Cosavirus (previously termed Dekavirus), methods of detecting the Cosavirus and diagnosing Cosavirus infection, methods of treating or preventing Cosavirus infection, and methods for identifying anti-Cosavirus compounds.
    Type: Grant
    Filed: March 19, 2009
    Date of Patent: March 17, 2015
    Assignee: Blood Systems, Inc.
    Inventors: Eric Delwart, Amit Kapoor, Joseph Victoria
  • Publication number: 20150044671
    Abstract: The invention relates to a method for detection of enterohemorrhagic Escherichia coli (EHEC), a probe for/the detection of enterohemorrhagic Escherichia coli (EHEC), sequences for the fragment of the gene encoding the Shiga toxin, the use of probes and sequences. More particularly, the invention relates to a method of visual detection and diagnostics of enterohemorrhagic Escherichia coli (EHEC) by detecting the signal from a probe using a transilluminator that emits UV radiation. Invention also relates to a method of visual detection and diagnosis of any other pathogen and/or biological material by detecting the signal from a probe using a transilluminator that emits UV radiation.
    Type: Application
    Filed: September 7, 2012
    Publication date: February 12, 2015
    Applicant: 3G THERAPEUTICS INC.
    Inventors: Grzegorz Wegrzyn, Bozena Nejman-Falenczyk
  • Publication number: 20150038353
    Abstract: The present invention provides a molecular marker for detecting Nosema infection, comprising at least one sequence selected from a gene sequence or a complementary sequence thereof of SR-22, SR-28, SR-71, and SR-85. By designing specific primer sets based on the sequences of molecular marker, the early stage and latent infections of Nosema can be detected, and the infection levels can also be determined.
    Type: Application
    Filed: April 30, 2014
    Publication date: February 5, 2015
    Applicant: National Taiwan University
    Inventors: Chu-Fang LO, Chung-Hsiung WANG, Yu-Shin NAI
  • Publication number: 20150037364
    Abstract: Baboon Adenovirus (BaAdV)-2/4 and BaAdV-3 are disclosed herein. BaAdV-2/4 and BaAdV-3 polynucleotide, polypeptides and antibodies that specifically bind BaAdV-2/4 and/or BaAdV-3 are disclosed. Methods are disclosed for detecting BaAdV-2/4 and BaAdV-3. Methods are also disclosed for treating, preventing, and inducing an immune response to BaAdV-2/4 and/or BaAdV-3. Kits are also provided.
    Type: Application
    Filed: January 15, 2014
    Publication date: February 5, 2015
    Inventors: Charles Chiu, Jean Patterson, Mary Michelle Leland, Kenneth Dee Carey, Dean Ehrdman
  • Publication number: 20150038348
    Abstract: In alternative embodiments, the invention provides products of manufacture and compositions, e.g., nucleic acid probes, for use as identifying agents or indicators to detect the presence of a hydrocarbon in a sample, e.g., in marine sediments, muds, sands and the like, or in a solution, e.g., an aqueous solution, such as a production water, fresh water, underground water or seawater. In alternative embodiments, the invention provides compositions, e.g., nucleic acid probes or primers or primer pairs, for use as sensors and/or identifying agents to detect the presence of a hydrocarbon in a sample (e.g., in fresh water, underground water or seawater, or a marine mud, sand or sediment), where the presence of the hydrocarbon indicates e.g., the presence of a subsurface oil, petroleum or gas accumulation or deposit. In alternative embodiments, the invention provides compositions and methods for use as tools for offshore oil exploration activities.
    Type: Application
    Filed: July 29, 2011
    Publication date: February 5, 2015
    Applicant: TAXON BIOSCIENCES, INC.
    Inventors: Matthew Ashby, Dago Dimster-Denk, Ulrika Elisa Lidstrom
  • Patent number: 8945850
    Abstract: The present invention relates to a method for the specific detection and/or identification of Staphylococcus species, in particular Staphylococcus aureus, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Staphylococcus species, in particular of S. aureus, in a biological sample. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Staphylococcus species in a sample.
    Type: Grant
    Filed: April 18, 2008
    Date of Patent: February 3, 2015
    Assignees: Fujirebio Europe N.V., Roche Diagnostics GmbH
    Inventors: Sofie Claeys, Geert Jannes, Gerd Haberhausen, Thomas Emrich, Lia Verdoodt
  • Publication number: 20150030587
    Abstract: Methods of treating a head and neck cancer are disclosed.
    Type: Application
    Filed: February 11, 2014
    Publication date: January 29, 2015
    Applicant: FOUNDATION MEDICINE, INC.
    Inventors: Chris Hendrik Boshoff, Timothy Robert Fenton, Matthias Alexander Lechner, Philip James Stephens, Matthew J. Hawryluk, Garrett Michael Frampton, Roman Yelensky
  • Publication number: 20150031015
    Abstract: The present invention relates to methods for identifying and/or distinguishing polioviral strains, in particular polioviral strains used in vaccine production. The methods are based on selective hybridisation with oligonucleotides, i.e. primers and/or probes, that allow to distinguish between closely related but different polioviral strains on the basis of nucleotidepolymorphisms existing between those polioviral strains. Preferably, the methods employ amplification or amplification-ligation assays for detecting the selective hybridisation. The invention further relates to oligonucleotides for use in the methods of the invention and kits comprising such oligonucleotides and optionally enzymes and buffers for carrying out the methods of the invention.
    Type: Application
    Filed: December 14, 2012
    Publication date: January 29, 2015
    Applicants: en Sport
    Inventors: Bernardus Metz, Olaf Egidius Marie Nijst, Justin Johannes Mouthaan, Dirk Ronald Mekkes
  • Patent number: 8932832
    Abstract: Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and de
    Type: Grant
    Filed: August 26, 2011
    Date of Patent: January 13, 2015
    Assignee: Medical Diagnostic Laboratories, LLC
    Inventors: Martin E. Adelson, Melanie Feola, Jason Trama, Eli Mordechai
  • Patent number: 8927701
    Abstract: A variant phylogenetic group of HPIV-2, more particularly a novel variant phylogenetic sub-group of HPIV-2, and a means for diagnosing HPIV-2 which take into account this novel group and this novel sub-group.
    Type: Grant
    Filed: July 18, 2008
    Date of Patent: January 6, 2015
    Assignees: Centre National de la Recherche Scientifique, Les Hospices Civils de Lyon
    Inventors: Bruno Lina, Olivier Terrier, Danielle Françoise Thouvenot
  • Patent number: 8927703
    Abstract: The present invention provides compositions, methods and kits for the species-specific detection of Pseudomonas aeruginosa.
    Type: Grant
    Filed: April 2, 2008
    Date of Patent: January 6, 2015
    Assignee: Gen-Probe Incorporated
    Inventors: Shannon K. Kaplan, Kristin Livezey, Jennifer J. Bungo, James J. Hogan
  • Publication number: 20140371441
    Abstract: This invention relates to a rapid method for detection and characterization of Escherichia coli bacteria serotype O157:H7 based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect E. coli O157:H7 in a food or water sample, such as a beef enrichment. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.
    Type: Application
    Filed: September 2, 2014
    Publication date: December 18, 2014
    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY
    Inventor: Frank R. BURNS
  • Publication number: 20140363811
    Abstract: The invention provides nucleic acids, collections of nucleic acids, supports, assay kits, and methods for the sensitive and specific detection of microorganisms in a foodstuff. The nucleic acid comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-15 and having a length of no more than 35 nucleotides.
    Type: Application
    Filed: June 6, 2013
    Publication date: December 11, 2014
    Inventors: Yu Wang, Yun Bao
  • Patent number: 8901287
    Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA:RNA hybrids which can be detected by a variety of methods.
    Type: Grant
    Filed: October 9, 2009
    Date of Patent: December 2, 2014
    Assignee: Qiagen Gaithersburg, Inc.
    Inventors: Irina Nazarenko, Attila Lorincz, Paul Eder, Brian Lowe, Richard Mallonee, Ha Thai
  • Publication number: 20140349276
    Abstract: This disclosure describes a structured polynucleotide, devices that include the structured polynucleotide, and methods involving the structured polynucleotide and/or devices. Generally, the structured polynucleotide includes five domains. A first domain acts as a toehold for an input DNA logic gate to initiate binding to an SCS biomolecule. A second domain acts as a substrate recognition sequence for an upstream DNA logic gate. A third domain acts as a toehold for a output DNA logic gate to initiate binding of the SCS biomolecule to the gate. A fourth domain acts as an effector sequence to alter the state of the output logic gate. A fifth domain acts as a cage sequence to lock the effector sequence in an inactive state until an input gate binds to the structured polynucleotide.
    Type: Application
    Filed: May 21, 2014
    Publication date: November 27, 2014
    Applicant: STC.UNM
    Inventors: CARL BROWN III, STEVEN WAYDE GRAVES, DARKO STEFANOVIC, MATTHEW RICHARD LAKIN
  • Patent number: 8895247
    Abstract: The present invention describes a method for detection of human papillomavirus (HPV) types and a kit for detection of said HPV types.
    Type: Grant
    Filed: April 22, 2010
    Date of Patent: November 25, 2014
    Assignee: Allum-Jenkins AS
    Inventors: Andrew Jenkins, Anne-Gry Allum, Linda Strand
  • Publication number: 20140335519
    Abstract: Oligomers useful for determining the presence of Trichomonas vaginalis in a test sample.
    Type: Application
    Filed: July 21, 2014
    Publication date: November 13, 2014
    Inventors: Barbara Susan WEINBAUM, Janel M. DOCKTER, Astrid R.W. SCHRODER
  • Patent number: 8883423
    Abstract: This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.
    Type: Grant
    Filed: October 11, 2012
    Date of Patent: November 11, 2014
    Assignee: T2 Biosystems, Inc.
    Inventor: Lori A. Neely
  • Publication number: 20140323710
    Abstract: This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.
    Type: Application
    Filed: July 8, 2014
    Publication date: October 30, 2014
    Inventors: Stephen Varkey, Daniel R. DeMarco, Mark A. Jensen
  • Publication number: 20140315745
    Abstract: Processes for detecting Dengue virus (DENV) nucleic acid in a sample are provided including producing an amplification product by amplifying DENV nucleotide sequence and detection of an amplification by hybridization of a probe or other technique. The processes use primers or probes with introduced mutations and or degenerate bases that provide excellent superiority in detection and serotyping of DENV in a sample.
    Type: Application
    Filed: October 25, 2012
    Publication date: October 23, 2014
    Inventors: Jorge L. Munoz-Jordan, Edgardo Vergne, Gilberto Santiago
  • Publication number: 20140315750
    Abstract: A process for detecting norovirus nucleic acid in a sample is provided including producing an amplification product by amplifying a norovirus nucleotide sequence using a forward primer of SEQ ID NO: 1, 4, or 10 and a reverse primer of SEQ ID NO: 2, 5, or 11, and detecting the amplification product to detect norovirus in the sample. Also provided are reagents and methods for detecting and distinguishing GI or Gil norovirus from other infectious agents. A kit is provided for detecting and quantifying norovirus in a sample.
    Type: Application
    Filed: November 15, 2012
    Publication date: October 23, 2014
    Applicants: and Human Services
    Inventors: Jan Vinje, Nicole Gregoricus, Preeti Chhabra, Leslie Barclay
  • Patent number: 8859203
    Abstract: The present invention relates to a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG.
    Type: Grant
    Filed: June 27, 2003
    Date of Patent: October 14, 2014
    Assignee: Fujirebio Europe N.V.
    Inventors: Lieven Stuyver, Rudi Rossau, Geert Maertens
  • Publication number: 20140294892
    Abstract: The present invention relates to attenuated, immunogenic West Nile virus chimeras built on a dengue virus backbone for the production of immunogenic, live, attenuated West Nile virus vaccines.
    Type: Application
    Filed: June 16, 2014
    Publication date: October 2, 2014
    Inventors: Alexander G. Pletnev, Joseph R. Putnak, Robert M. Chanock, Brian R. Murphy, Stephen S. Whitehead, Joseph E. Blaney
  • Publication number: 20140295409
    Abstract: The present invention provides an isolated mammalian negative strand RNA virus, metapneumovirus (MPV), within the sub-family Pneumoviridae, of the family Paramyxoviridae. The invention also provides isolated mammalian negative strand RNA viruses identifiable as phylogenetically corresponding or relating to the genus Metapneumovirus and components thereof. In particular the invention provides a mammalian MPV, subgroups and variants thereof. The invention relates to genomic nucleotide sequences of different isolates of mammalian metapneumoviruses, in particular human metapneumoviruses. The invention relates to the use of the sequence information of different isolates of mammalian metapneumoviruses for diagnostic and therapeutic methods. The present invention relates to nucleotide sequences encoding the genome of a metapneumovirus or a portion thereof, including both mammalian and avian metapneumovirus. The invention further encompasses chimeric or recombinant viruses encoded by said nucleotide sequences.
    Type: Application
    Filed: May 5, 2014
    Publication date: October 2, 2014
    Inventors: Ronaldus Adrianus Maria Fouchier, Bernadetta Gerarda Van Den Hoogen, Albertus D M E Osterhaus, Aurelia Haller, Roderick Tang
  • Publication number: 20140296110
    Abstract: Described herein are primers and probes useful for the binding, detecting, differentiating, isolating, and sequencing of influenza A, influenza B, 2009 influenza A/H1N1, and a 2009 influenza A/H1N1 RNA sequence mutation associated with oseltamivir resistance.
    Type: Application
    Filed: June 17, 2014
    Publication date: October 2, 2014
    Inventors: James R. Hully, David L. Dolinger, Alice A. Jacobs, Damien Slater, Heather L.B. Kiefer, Chesley Leslin, Juan Manuel Anzola
  • Publication number: 20140272966
    Abstract: A method for diagnosing increased risk of developing Methicillin-resistant Staphylococcus aureus (MRSA) hospital-acquired (HA-MRSA) or community-acquired MRSA (CA-MRSA) which includes obtaining a biological sample from a subject, detecting in the sample a single nucleotide polymorphism (SNP) in the FAM129B gene at position 17 of SEQ ID NO 1, and comparing the nucleotide at position 17 of SEQ ID NO. 1 in the sample with the nucleotide at position 17 in SEQ ID NO. 1, wherein an adenine at position 17 of SEQ ID NO. 1 in the sample indicates an increased risk of developing MRSA or CA-MRSA in the subject.
    Type: Application
    Filed: March 13, 2014
    Publication date: September 18, 2014
    Applicant: William Beaumont Hospital
    Inventor: Matthew Sims
  • Patent number: 8835106
    Abstract: The present invention relates to a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG.
    Type: Grant
    Filed: May 22, 2007
    Date of Patent: September 16, 2014
    Assignee: Fujirebio Europe N.V.
    Inventors: Lieven Stuyver, Rudi Rossau, Geert Maertens
  • Patent number: 8835117
    Abstract: Methods of detecting Chlamydophila, including differentiating between species of Chlamydophila and/or strains of Chlamydophila psittaci are disclosed, for example to detect and genotype a Chlamydophila psittaci infection. A sample suspected of containing a nucleic acid of a Chlamydophila, is screened for the presence of that nucleic acid. The presence of the Chlamydophila nucleic acid indicates the presence of the Chlamydophila bacterium. Determining whether a Chlamydophila nucleic acid is present in a sample can be accomplished by detecting hybridization between a Chlamydophila specific primer, a Chlamydophila psittaci specific primer, and/or a Chlamydophila psittaci genotype-specific primer and the Chlamydophila nucleic acid containing sample. Thus, primers for the detection, species-specific and/or genotype-specific identification of Chlamydophila psittaci are disclosed. Kits that contain the disclosed primers also are disclosed.
    Type: Grant
    Filed: May 28, 2010
    Date of Patent: September 16, 2014
    Assignee: The United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and Prevention
    Inventors: Stephanie L. Mitchell, Jonas M. Winchell
  • Publication number: 20140256583
    Abstract: The invention relates to human endogenous retrovirus env (HERV-WL) polypeptides, nucleotide sequences, HERV-WL antibodies, methods to detect cancer, and methods to determine the effectiveness of the treatment of cancer.
    Type: Application
    Filed: March 5, 2014
    Publication date: September 11, 2014
    Applicant: Rutgers, The State University of New Jersey
    Inventors: Vincent K. Tsiagbe, Yu Li