Labels Or Markers Utilized (e.g., Radiotracer, Affinity, Fluoroescent, Phosphorescent, Markers, Etc.) Patents (Class 536/25.32)
  • Patent number: 9428797
    Abstract: This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
    Type: Grant
    Filed: July 29, 2004
    Date of Patent: August 30, 2016
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Elazar Rabbani, Jannis G. Stavrianopoulos, James J. Donegan, Jack Coleman
  • Patent number: 9347092
    Abstract: The present invention provides a solid support which is preferably a bead comprising at least two sequence specific amplification primers wherein at least one primer is bound to the support with an inducible cleavable linker. The present invention also provides various method for preparing a solid support comprising at least two sequence specific primers, further characterized in that at least one of the primers is cleavable.
    Type: Grant
    Filed: February 15, 2010
    Date of Patent: May 24, 2016
    Assignee: ROCHE MOLECULAR SYSTEM, INC.
    Inventors: Angelika Roesler, Thomas Froehlich, Dieter Heindl
  • Patent number: 9243284
    Abstract: Nucleotide triphosphate probes containing a molecular and/or atomic tag on a ? and/or ? phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: January 26, 2016
    Assignee: Life Technologies Corporation
    Inventors: Susan H. Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Patent number: 9212387
    Abstract: Provided is a detection kit for detecting target genes of miRNA. Also provided is a method of detecting target genes of miRNA in a simple manner without the need for performing a transfection operation of a gene into cells via a vector. The detection kit is a detection kit for target genes of microRNA, including a cell extraction reagent, and a labeling reagent for microRNA or labeled microRNA, and further including a reaction reagent for the labeling substance for microRNA. mRNA corresponding to target genes of miRNA can be easily pulled down by producing a cell extract under mild conditions, adding labeled miRNA to the cell extract, and recovering the labeling substance. cDNA is produced from the pulled down mRNA to detect target genes of miRNA.
    Type: Grant
    Filed: June 15, 2011
    Date of Patent: December 15, 2015
    Assignee: National University Corporation Okayama University
    Inventors: Mamoru Ouchida, Sachio Ito
  • Patent number: 9040674
    Abstract: The present disclosure provides reagents that can be used to label synthetic oligonucleotides with rhodamine dyes or dye networks that contain rhodamine dyes.
    Type: Grant
    Filed: April 2, 2007
    Date of Patent: May 26, 2015
    Assignee: Applied Biosystems, LLC
    Inventors: Scott C. Benson, Ruiming N. Zou, Krishna G. Upadhya, Paul M. Kenney, Jonathan M. Cassel
  • Patent number: 9035036
    Abstract: Disclosed herein are methods and reagents for determining the responsiveness of cancer to an epidermal growth factor receptor (EGFR) targeting treatment. The detection of these mutations will allow for the administration of gefitinib, erlotinib and other tyrosine kinase inhibitors to those patients most likely to respond to the drug.
    Type: Grant
    Filed: May 17, 2013
    Date of Patent: May 19, 2015
    Assignees: The General Hospital, Dana-Farber Cancer Institute, Inc.
    Inventors: Daphne Winifred Bell, Daniel A. Haber, Pasi Antero Janne, Bruce E. Johnson, Thomas J. Lynch, Matthew Meyerson, Juan Guillermo Paez, William R. Sellers, Jeffrey E. Settleman, Raffaella Sordella
  • Patent number: 9029526
    Abstract: Polynucleotides and polypeptides which participate in influenza virus infection of cells and nucleic acid molecules, which include a polynucleotide sequence capable of specifically binding the polypeptides of the present invention. Also provided are methods of using such nucleic acid molecules, polynucleotides and antibodies directed thereagainst for diagnosing, treating and preventing influenza virus infection.
    Type: Grant
    Filed: November 19, 2012
    Date of Patent: May 12, 2015
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Ruth Arnon, Sung-Ho Jeon, Basak Kayhan, Tamar Ben-Yedidia
  • Publication number: 20150126723
    Abstract: The present invention is generally related to composition, kits and methods for synthesizing nucleic acid molecules and particularly for synthesizing labeled nucleic acid molecules. Specifically, the invention relates to methods, kits and compositions for synthesizing indirectly labeled nucleic acid molecules. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection, diagnostics, and array analysis.
    Type: Application
    Filed: November 4, 2014
    Publication date: May 7, 2015
    Inventors: Jun LEE, Weidong ZHENG
  • Publication number: 20150119565
    Abstract: The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphoramidites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides, and deuterated RNA's of defined sequences which can exhibit biochemically useful and biologically valuable properties, thus having potential for therapeutic uses.
    Type: Application
    Filed: October 10, 2014
    Publication date: April 30, 2015
    Inventors: Suresh C. Srivastava, Amy Yasko
  • Publication number: 20150099277
    Abstract: Provided herein, inter alia, are compositions and methods of synthesis and detection of tetrazines and diazonorcaradienes.
    Type: Application
    Filed: October 6, 2014
    Publication date: April 9, 2015
    Inventors: Neal K. Devaraj, Jun Yang, Jolita Seckute
  • Patent number: 8981078
    Abstract: An agent for inhibiting translesion DNA replication comprises a non-natural adenine ribose analog represented by those as set forth in FIG. 1.
    Type: Grant
    Filed: January 4, 2012
    Date of Patent: March 17, 2015
    Assignee: Case Western Reserve University
    Inventors: Anthony J. Berdis, Irene Lee, Xuemei Zhang
  • Publication number: 20150038691
    Abstract: A mixed polynucleotide includes a first double stranded (ds) portion, a second portion including at least one single stranded (ss) portion, and a third ds portion. The second portion connects the first ds portion and the third ds portion to provide a modified polynucleotide.
    Type: Application
    Filed: August 28, 2013
    Publication date: February 5, 2015
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Binquan Luan, Ajay K. Royyuru, Gustavo A. Stolovitzky, Chao Wang, Deqiang Wang
  • Patent number: 8945851
    Abstract: The present invention relates to a kit for detection of association of a peripheral cellular membrane binding protein with cellular membranes in living cells and methods thereof. The kit includes a first nucleic acid construct comprising a first nucleic acid molecule encoding a first fusion protein comprising a peripheral cellular membrane binding protein or membrane binding domain thereof operatively coupled to DNA binding and transactivation domains of a naturally occurring or chimeric transcription factor and a first promoter operatively associated with the first nucleic acid molecule. A second nucleic acid construct comprises a second nucleic acid molecule encoding a reporter protein and a second promoter responsive to the DNA binding and transactivation domains of the first fusion protein. The second promoter is operatively associated with the second nucleic acid molecule. Activation of the second promoter results in expression of the reporter protein. Also disclosed is a transgenic non-human animal.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: February 3, 2015
    Assignee: New York University
    Inventors: Mark R. Philips, Nicole Fehrenbacher, Joseph Wynne
  • Patent number: 8916346
    Abstract: The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.
    Type: Grant
    Filed: December 6, 2011
    Date of Patent: December 23, 2014
    Assignee: Marker Gene Technology, Inc.
    Inventors: John J. Naleway, Ying Jiang, Ryan Link-Cole
  • Publication number: 20140349282
    Abstract: The invention pertains to a near-infrared fluorescent dye that is cell permeable and can be attached to selected proteins in living cells. The dye has the general formula or its corresponding spirolactone wherein Y is chosen from the group consisting of Si, Ge and Sn; R0 is —COO? or COOH; R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13, R14, R15 and R16 are substituents, including hydrogen, independently from each other. The dye (i) absorbs and emits light at wavelengths above 600 nm; (ii) possesses high photostability; (iii) has high extinction coefficients and high quantum yields; (iv) can be derivatized with different molecules; and (v) is membrane-permeable and shows minimal background binding to biomolecules and biomolecular structures.
    Type: Application
    Filed: August 26, 2011
    Publication date: November 27, 2014
    Inventors: Keitaro Umezawa, Lukinavicius Grazvydas, Kai Johnsson
  • Patent number: 8889851
    Abstract: A reagent for oligonucleotide synthesis or purification, wherein the reagent has a structure of: X—C—L—H??(Formula A) wherein X is a phosphoramidite group, an H-phosphonate group, an acetal group, or an isocyanate; C is a direct bond or a cleavable adaptor represented by —Ca—Cb—; L is a hydrocarbyl chain; and H is a terminal alkyne or an activated cyclooctyne. The reagent of Formula (A) can be used in the synthesis and purification of oligonucleotides.
    Type: Grant
    Filed: November 6, 2012
    Date of Patent: November 18, 2014
    Assignee: Agilent Technologies, Inc.
    Inventors: Emily Marine Leproust, Jeremy Lackey
  • Patent number: 8889843
    Abstract: To provide an excellent dimer amidite which can be subjected to purification, preferably, whose protective groups can be removed under mild conditions, and a method for synthesizing a nucleic acid using the dimer amidite, a dimer amidite having a structure represented by the following General Formula (1) and a method for synthesizing a nucleic acid including performing condensation reaction of the dimer amidite are provided: wherein in General Formula (1), R1 and R2 each independently represent any one of groups selected from General formulas (2) to (4) and Structural Formulas (12) to (15) with a compound where R1 and R2 are each represent Structural Formulas (12) being excluded: ?and wherein in the General Formulas (2) to (4), R3 represents any one group represented by the following Structural Formulas (16) to (25):
    Type: Grant
    Filed: February 26, 2010
    Date of Patent: November 18, 2014
    Assignee: Apta Biosciences Ltd.
    Inventor: Tsuyoshi Fujihara
  • Publication number: 20140329234
    Abstract: Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system.
    Type: Application
    Filed: June 3, 2014
    Publication date: November 6, 2014
    Applicants: AMINA CELL METROLOGY, THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: BARRY S. COOPERMAN, ZEEV SMILANSKY, YALE E. GOLDMAN, DONGLI PAN
  • Patent number: 8865407
    Abstract: This document provides methods and materials for detecting target nucleic acid. For example, methods and materials for detecting the presence or absence of target nucleic acid, methods and materials for detecting the amount of target nucleic acid present within a sample, kits for detecting the presence or absence of target nucleic acid, kits for detecting the amount of target nucleic acid present within a sample, and methods for making such kits are provided.
    Type: Grant
    Filed: December 19, 2013
    Date of Patent: October 21, 2014
    Assignee: Cascade Biosystems, Inc.
    Inventors: Kenneth D. Smith, Nina Yazvenko, Mariya Smit
  • Patent number: 8859266
    Abstract: The present disclosure encompasses systems, and their methods of use, for detecting a target analyte. The systems include a first and second oligonucleotide probe that associate together to form a complex that binds to a target analyte; a cleavable reporter molecule that binds to the complex; and cleaving agent.
    Type: Grant
    Filed: April 28, 2011
    Date of Patent: October 14, 2014
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventors: Yuliva V. Gerasimova, Dmitry M. Kolpashchikov
  • Patent number: 8859754
    Abstract: The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphoramidites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides, and deuterated RNA's of defined sequences which can exhibit biochemically useful and biologically valuable properties, thus having potential for therapeutic uses.
    Type: Grant
    Filed: July 31, 2012
    Date of Patent: October 14, 2014
    Assignee: Ased, LLC
    Inventors: Suresh C. Srivastava, Amy Yasko
  • Patent number: 8859753
    Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. In particular, the methods include a [3+2] cycloaddition between a nucleotide analog incorporated into a nucleic acid polymer and a reagent attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.
    Type: Grant
    Filed: October 28, 2010
    Date of Patent: October 14, 2014
    Assignee: President and Fellows of Harvard College
    Inventors: Adrian Salic, Timothy J. Mitchison
  • Patent number: 8815509
    Abstract: The inventors have developed a rapid and sensitive fluorescence-based assay to quantify dNTPs. This assay relies on the principle that incorporation of a limiting dNTP is required for primer-extension and polymerase-mediated 5-3? exonuclease hydrolysis of a quenched fluorophore-labeled probe resulting in fluorescence. The concentration of limiting dNTPs is directly proportional to the fluorescence generated. This assay has important applications in research that investigates the influence of pathological conditions or pharmacological agents on dNTP biosynthesis and regulation.
    Type: Grant
    Filed: June 14, 2012
    Date of Patent: August 26, 2014
    Assignee: University of Southern California
    Inventors: Peter M. Wilson, Robert D. Ladner
  • Patent number: 8808683
    Abstract: The present invention relates to a genetically modified bacterium of the species Listeria monocytogenes, wherein the genomic locus of the transcriptional factor PrfA has been deleted, characterized in that it comprises on genomic level an artificial sequence that acts as internal amplification control, the use of this bacterium as well as a method for detecting and determining qualitatively and/or quantitatively the occurrence of wild type Listeria monocytogenes in a sample suspected to be contaminated with said micro-organism and a kit.
    Type: Grant
    Filed: April 29, 2011
    Date of Patent: August 19, 2014
    Assignee: Merck Patent GmbH
    Inventors: Peter Rossmanith, Karin Fruehwirth, Martin Wagner, Sabine Fuchs
  • Patent number: 8765934
    Abstract: A method for conjugating a nucleic acid with a molecule is provided. The method includes steps of (a) reacting the nucleic acid having a 5?-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a nucleophile thereinto to react the intermediate with the nucleophile.
    Type: Grant
    Filed: July 22, 2011
    Date of Patent: July 1, 2014
    Assignee: Kaohsiung Medical University
    Inventors: Tzu-Pin Wang, Yi-Jhang Ciou
  • Publication number: 20140178877
    Abstract: The present invention is directed to methods for generating a labeled oligonucleotide probe that contains a fluorescent rhodamine-derived dye for use in PCR reactions to detect a target nucleic acid.
    Type: Application
    Filed: December 6, 2013
    Publication date: June 26, 2014
    Applicant: Roche Molecular Systems, Inc.
    Inventors: Concordio Anacleto, Teodorica Bugawan, Nancy Schoenbrunner
  • Patent number: 8741567
    Abstract: The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and/or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule.
    Type: Grant
    Filed: April 27, 2011
    Date of Patent: June 3, 2014
    Assignee: The University of Chicago
    Inventors: Chuan He, Chunxiao Song
  • Patent number: 8680260
    Abstract: The present invention provides an 18F-labeled azide compound usable in the Huisgen reaction which enables 18F-labeling although only a small quantity of alkyne compound is available as a counterpart substrate, more specifically the 18F-labeled azide compound enabling the PET to be applied to peptides or oligonucleotides and enabling the 18F-labeling of any sites of oligonucleotide other than the 5? end or 3? end thereof, a reagent for 18F-labeling, and a method for 18F-labeling of an alkyne compound using the same.
    Type: Grant
    Filed: May 14, 2010
    Date of Patent: March 25, 2014
    Assignee: Riken
    Inventors: Yasuyoshi Watanabe, Masaaki Suzuki, Hisashi Doi, Takeshi Kuboyama, Satoshi Obika, Takeshi Imanishi
  • Publication number: 20140065605
    Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation.
    Type: Application
    Filed: August 21, 2013
    Publication date: March 6, 2014
    Applicants: Life Technologies Corporation, President and Fellows of Harvard College
    Inventors: Kyle R. Gee, Brian Agnew, Adrian Salic, Timothy J. Mitchison
  • Patent number: 8652851
    Abstract: An oligonucleotide-based molecular probe includes at least one pin loop, the pin loop including a loop sequence complementary to a target sequence. A first stem sequence is attached to one end of the pin loop, the first stem having at least one fluorescent label attached thereto. A second stem sequence is attached to the other end of the pin loop. The second stem has a plurality of quencher molecules attached thereto.
    Type: Grant
    Filed: June 17, 2005
    Date of Patent: February 18, 2014
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Weihong Tan, Chaoyong Yang
  • Patent number: 8649983
    Abstract: The present invention relates to a method and to compounds useable in the method for analysis of cells for the presence of an analyte and sorting the cells on the basis of the analyte. The compounds used in the method are optically detectable because of the content of a fluorochrome and contain a binder fraction which can specifically bind an analyte, in particular an oligonucleotide.
    Type: Grant
    Filed: July 15, 2009
    Date of Patent: February 11, 2014
    Assignee: Masterrind, GmbH
    Inventors: Detlef Rath, Wilfried Kues, Ulrike Taylor, Stephan Barcikowski, Svea Petersen
  • Publication number: 20140039175
    Abstract: The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphoramidites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides, and deuterated RNA's of defined sequences which can exhibit biochemically useful and biologically valuable properties, thus having potential for therapeutic uses.
    Type: Application
    Filed: July 31, 2012
    Publication date: February 6, 2014
    Inventors: Suresh C. Srivastava, Amy Yasko
  • Patent number: 8642754
    Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.
    Type: Grant
    Filed: February 13, 2009
    Date of Patent: February 4, 2014
    Assignee: Japan Science and Technology Agency
    Inventors: Kenzo Fujimoto, Masayuki Ogino, Yoshinaga Yoshimura
  • Patent number: 8637322
    Abstract: The present invention relates to a method for labeling biological molecules of interest contained in a biological sample, consisting in: a) providing a reaction vessel, b) immobilizing capture molecules, which are capable of binding a label of the biological molecules of interest, on a solid support of the vessel, c) introducing the biological sample but also at least one label of the biological molecules of interest into said vessel and optionally any ingredient required for labeling or prelabeling the molecules of interest, d) incubating the vessel content and immobilizing the label which is not reacted with the molecules of interest by binding to the capture molecules, and e) using the labeled molecules of interest for subsequent steps. A method for treating a biological sample is also disclosed. Said invention is preferably used in a manual or automated method for purifying nucleic acids.
    Type: Grant
    Filed: May 31, 2006
    Date of Patent: January 28, 2014
    Assignee: Biomerieux
    Inventors: Eloy Bernal-Mendez, Ali Laayoun, Lionel Menou
  • Publication number: 20140024819
    Abstract: The present invention relates to a method of preparing triphosphate-modified oligonucleotides using a capture tag. The method allows the synthesis and purification of triphosphate-modified oligonucleotides in high yield and purity suitable for pharmaceutical applications.
    Type: Application
    Filed: March 28, 2012
    Publication date: January 23, 2014
    Applicant: RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITAET BONN
    Inventors: Janos Ludwig, Marion Goldeck, Brian Sproat
  • Patent number: 8592202
    Abstract: The invention generally provides molecular biosensors. In particular, the invention provides molecular biosensors having one or more aptamers. The molecular biosensors are useful in several methods including in the identification and quantification of target molecules.
    Type: Grant
    Filed: December 6, 2010
    Date of Patent: November 26, 2013
    Assignee: Saint Louis University
    Inventors: Tomasz Heyduk, Ewa Heyduk, Eric Knoll
  • Patent number: 8547533
    Abstract: Composite probes for super resolution optical techniques using super resolution via transiently activated quenchers (STAQ) include a donor moiety and an acceptor moiety joined by a linker, wherein the acceptor moiety, when excited by incident radiation, is excited to a state which, for example, absorbs in the donor emission region, such that the acceptor moiety in its excited state quenches at least a portion of the donor moiety emission. Other transiently activated quenching mechanisms and moieties could accomplish the same task by reducing donor population. Also disclosed are methods for irradiating a selected region of a target material including the composite probe, wherein the composite probe enables improved resolution by point spread function modification and/or nanoscale chemical reactions.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: October 1, 2013
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Jay R. Knutson, Gary L. Griffiths
  • Publication number: 20130253179
    Abstract: The present invention relates to a process of functionalising at least one ribonucleic acid (RNA) molecule which comprises the following steps: a) having at least: a binding molecule constituted by an isatoic anhydride or a derivative thereof, a group of interest, and a binding arm linking the binding molecule with the group of interest, b) reacting the anhydride function of the binding molecule with at least one hydroxyl group in: position 2? of the ribose of one of the RNA nucleotides, and/or position(s) 2? and/or 3? of the ribose of the nucleotide at the 3? terminal end of the RNA, and c) obtaining an anthranilate linking, via the binding arm, the RNA to the group of interest. The invention also relates to a functionalising reagent able to be used in such processes, a functionalised biological RNA molecule capable of being obtained by these processes and a kit for detecting a target RNA molecule comprising such a reagent.
    Type: Application
    Filed: December 5, 2011
    Publication date: September 26, 2013
    Inventors: Arnaud Burr, Ali Laayoun, Alain Laurent
  • Publication number: 20130251637
    Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
    Type: Application
    Filed: December 20, 2011
    Publication date: September 26, 2013
    Applicants: DYOMICS GMBH, PIERCE BIOTECHNOLOGY, INC.
    Inventors: Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa Dworecki, Frank G. Lehmann
  • Publication number: 20130231473
    Abstract: A method for joining oligonucleotides. The method includes joining together one or more oligonucleotides by reacting an alkyne group lined to an oligonucleotide with an azide group linked to an oligonucleotide to form a triazole linkage. The alkyne group is a strained alkyne group. The method can include ligating together ends of one or more oligonucleotides or cross-linking strands of an oligonucleotide duplex. The methods described allow oligonucleotide strands to be ligated together without the need for a ligase enzyme.
    Type: Application
    Filed: March 1, 2012
    Publication date: September 5, 2013
    Inventors: Tom Brown, Afaf Helmy El-Sagheer
  • Patent number: 8524503
    Abstract: The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
    Type: Grant
    Filed: March 6, 2012
    Date of Patent: September 3, 2013
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Hye Eun Hoover, Wai-Yee Leung
  • Patent number: 8519153
    Abstract: The present application is directed to compounds of Formula I: wherein R1-R9, X, Y and Z are as defined in the application, and to the use of the compounds of Formula I, for example, for the fluorescent labeling of oligonucleotides.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: August 27, 2013
    Assignee: Brock University
    Inventors: Hongbin Yan, Kha Tram
  • Patent number: 8492536
    Abstract: A method for modifying nucleic acid bases by a chemical means, which enables the discrimination of every base species in plural species of bases in a nucleic acid comprising plural nucleotide units, while retaining the base sequence information of the nucleic acid. A nucleic acid base-modified product provided by the method. The nucleic acid base-modified product is essentially a single strand. In accordance with the invention, a novel means for sequencing a nucleic acid by a microscopic means is provided.
    Type: Grant
    Filed: August 8, 2008
    Date of Patent: July 23, 2013
    Assignees: Inter-University Research Institute Corporation National Institute of Natural Sciences, Nagayama IP Holdings, LLC
    Inventors: Masanori Kataoka, Kuniaki Nagayama
  • Patent number: 8440635
    Abstract: Oligonucleotide conjugates, where an oligonucleotide is covalently attached to an aromatic system, are provided. In particular embodiments the oligonucleotide is complementary to the RNA component of human telomerase and is covalently attached to a nucleobase via an optional linker. The conjugates inhibit telomerase enzyme activity.
    Type: Grant
    Filed: July 17, 2009
    Date of Patent: May 14, 2013
    Assignee: Geron Corporation
    Inventors: Sergei M. Gryaznov, Krisztina Pongracz, Richard L. Tolman, Gregg B. Morin
  • Patent number: 8435800
    Abstract: The present invention relates in general to labeling reagents useful for labeling biomolecules. In particular, the invention provides activated labeling reagents having the formula L-Ph, wherein L is an activated labeling molecule and Ph is a phenol. The invention further provides methods of preparing the labeling reagents, methods of using the labeling reagents for synthesizing a labeled biomolecule, kits that include reagents for labeling a biomolecule and kits containing labeled biomolecules.
    Type: Grant
    Filed: November 22, 2005
    Date of Patent: May 7, 2013
    Assignee: BioSight Ltd.
    Inventor: Stela Gengrinovitch
  • Patent number: 8394948
    Abstract: Novel CE-phosphoramidites and CPG reagents have been synthesized from a serinol backbone. These reagents are useful to introduce functional groups or directly label oligonucleotides. The versatile serinol scaffold allows for labeling at any position (5? or 3? termini, or any internal position) during automated DNA synthesis. Multiple labels or functional groups can be achieved by repetitive coupling cycles. Optimal spacer arms and protected label moieties have been specially designed. Further, the natural 3-carbon atom internucleotide phosphate distance is retained when inserted internally.
    Type: Grant
    Filed: September 28, 2010
    Date of Patent: March 12, 2013
    Assignees: Glen Research Corporation, Nelson Biotechnologies, Inc.
    Inventors: Paul S. Nelson, Hugh Mackie, Andrew Murphy
  • Patent number: 8361727
    Abstract: Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3?-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: January 29, 2013
    Assignee: Lasergen, Inc.
    Inventors: Weidong Wu, Vladislav A. Litosh, Brian P. Stupi, Michael L. Metzker
  • Patent number: 8362234
    Abstract: The present invention relates to a compound according to the formula: wherein R1, R2, R3, R4, R5, R6, R7, R8, Y, Z, n, s, and t are as defined herein. These compounds are useful for methods of solid phase synthesis.
    Type: Grant
    Filed: March 26, 2007
    Date of Patent: January 29, 2013
    Inventors: Paul J. Hatala, Markus Kurz
  • Patent number: 8357789
    Abstract: Polynucleotides and polypeptides which participate in influenza virus infection of cells and nucleic acid molecules, which include a polynucleotide sequence capable of specifically binding the polypeptides of the present invention. Also provided are methods of using such nucleic acid molecules, polynucleotides and antibodies directed thereagainst for diagnosing, treating and preventing influenza virus infection.
    Type: Grant
    Filed: September 18, 2009
    Date of Patent: January 22, 2013
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Ruth Arnon, Sung-Ho Jeon, Basak Kayhan, Tamar Ben-Yedidia
  • Patent number: 8334370
    Abstract: Provided are nucleic acid compositions modified with intercalating aromatic compounds attached directly or indirectly through a linker arm thereto. Modifications to the nucleotides or to the oligo- or polynucleotides involve the pentosyl moieties, the abasic moieties (without a base) and the base moieties. Useful property changes are effected and can be measured or detected when such modified nucleic acids compositions have hybridized specifically to target nucleic acids.
    Type: Grant
    Filed: October 5, 1992
    Date of Patent: December 18, 2012
    Assignee: Enzo Biochem, Inc.
    Inventors: Jannis Stavrianopoulos, Elazar Rabbani