Separation Or Purification Of Polynucleotides Or Oligonucleotides Patents (Class 536/25.4)
  • Patent number: 10407713
    Abstract: The present invention provides a special reagent for the pretreatment of sample materials. The pretreatment with the reagent according to the invention enables the isolation of nucleic acids by a uniform method from very diverse sample materials, in particular different bioprocess samples, even if the nucleic acids are only present in small amounts in the sample materials. The reagent used for the pretreatment comprises, as key component, at least one compound comprising an amino group. The invention further relates to methods of purification of nucleic acids, in which the sample material is pretreated correspondingly, and suitable kits.
    Type: Grant
    Filed: July 4, 2012
    Date of Patent: September 10, 2019
    Assignee: QIAGEN GmbH
    Inventor: Peter Porschewski
  • Patent number: 10227582
    Abstract: The present disclosure relates to systems and methods for nucleic acid isolation from cellular samples. In particular, the present disclosure provides systems and methods for lysing cells and recovering nucleic acids.
    Type: Grant
    Filed: May 9, 2016
    Date of Patent: March 12, 2019
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Thomas N. Chiesl, Michelle Toro, Steven Gerald Haupt
  • Patent number: 10201620
    Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.
    Type: Grant
    Filed: December 31, 2012
    Date of Patent: February 12, 2019
    Assignee: CELLSCRIPT, LLC
    Inventors: Judith Meis, Anthony Person, Cynthia Chin, Jerome Jendrisak, Gary Dahl
  • Patent number: 10184145
    Abstract: The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps: binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture; separating the solid phase with the bound extracellular nucleic acids; optionally washing the extracellular nucleic acids; optionally eluting extracellular nucleic acids from the solid phase. The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.
    Type: Grant
    Filed: September 25, 2012
    Date of Patent: January 22, 2019
    Assignee: QIAGEN GmbH
    Inventors: Martin Horlitz, Annette Nocon, Markus Sprenger-Haussels, Peter Grünefeld, Christoph Erbacher
  • Patent number: 10131935
    Abstract: Parallel isolation of a double-stranded nucleic acid and a single-stranded nucleic acid is possible from a sample that contains these acids, without separating the acids, by mixing the sample with a lysis buffer having high salt concentration or low salt concentration, or having a proteolytic enzyme. The sample that contains nucleic acid before its lysis, or the sample that has already been lysed or homogenized, is adjusted with a binding buffer in such a manner that the total nucleic acid is adsorbed onto a solid carrier. The binding buffer contains at least one non-ionic detergent in a high concentration. With the exception of the detergent, the sample contains no other non-acidic organic component miscible in water. The carrier with the adsorbed total nucleic acid is removed. The adsorbed total nucleic acid is washed and eluted.
    Type: Grant
    Filed: January 12, 2009
    Date of Patent: November 20, 2018
    Assignee: AJ INNUSCREEN GmbH
    Inventor: Timo Hillebrand
  • Patent number: 10087439
    Abstract: The invention is directed to compositions and methods for rapidly and efficiently extracting nucleic acids and/or targeted nucleic acids sequences from biological samples. The methods of the invention comprise combining the sample with a buffer and magnetic silicon beads and concentrating the beads with a magnet or other electrical field. Liquid may be removed, or not, and an alkaline buffer is added followed by magnetic carboxy beads in a binding buffer so that nucleic acids transfer to the carboxy beads, which can be easily and quickly isolated once again with a magnet. Total nucleic acid extraction is greatly enhanced. Extracted nucleic acids can be analyzed, for example, by PCR wherein the nucleic acids can be identified and characterized. Carboxy beads may also contain a ligand so as to target specific nucleic acid sequences. The invention is also directed to kits comprising the tools and compositions for performing the methods of the invention.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: October 2, 2018
    Assignee: Longhorn Vaccines and Diagnostics, LLC
    Inventors: Gerald W. Fischer, Luke T. Daum
  • Patent number: 9976136
    Abstract: The invention is directed to compositions and methods for rapidly and efficiently extracting nucleic acids and/or targeted nucleic acids sequences from biological samples. The methods of the invention comprise combining the sample with a buffer and magnetic silicon beads and concentrating the beads with a magnet or other electrical field. Liquid may be removed, or not, and an alkaline buffer is added followed by magnetic carboxy beads in a binding buffer so that nucleic acids transfer to the carboxy beads, which can be easily and quickly isolated once again with a magnet. Total nucleic acid extraction is greatly enhanced. Extracted nucleic acids can be analyzed, for example, by PCR wherein the nucleic acids can be identified and characterized. Carboxy beads may also contain a ligand so as to target specific nucleic acid sequences. The invention is also directed to kits comprising the tools and compositions for performing the methods of the invention.
    Type: Grant
    Filed: May 12, 2016
    Date of Patent: May 22, 2018
    Assignee: Longhorn Vaccines and Diagnostics, LLC
    Inventors: Gerald W. Fischer, Luke T. Daum
  • Patent number: 9976993
    Abstract: An apparatus for extracting an analyte from a liquid sample having a container with an entrance, an exit, and a passage therebetween for passage of a liquid sample containing an analyte, the container having a full diameter bed region and a reduced diameter bed region. The container includes a layered construction extending across the passage, having from top to bottom one or more of: (i) an upper flow distributor/support layer, (ii) an upper compression layer, (iii) an extraction layer of microparticulate extraction medium adjacent to the layer (ii), and (iv) a lower compression layer located adjacent to the extraction layer (iii), optionally including one or more air gap layers. At least some of the layers are located in the full diameter bed region, and some of the layers are located in the reduced diameter bed region. The apparatus may have a plurality of containers arranged in an array and/or in series so as to provide multi-stage filtration or extraction.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: May 22, 2018
    Assignee: TECAN SP, INC.
    Inventor: Philip A. Dimson
  • Patent number: 9907702
    Abstract: Wound dressing articles comprising a nonwoven web comprising a plurality of fibers having grafted pendant hydrophilic groups, methods that use high energy irradiation for making a plurality of fibers having grafted pendant hydrophilic groups, useful for making wound dressing articles.
    Type: Grant
    Filed: August 13, 2012
    Date of Patent: March 6, 2018
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Cary A. Kipke, John J. Rogers, Michael R. Berrigan, Clinton P. Waller, Jr., Douglas E. Weiss
  • Patent number: 9896682
    Abstract: Methods and compositions are described herein for protecting RNA from autocatalytic and divalent cation induced degradation in an aqueous solution.
    Type: Grant
    Filed: March 7, 2016
    Date of Patent: February 20, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Xiao-Song Gong, Cindy Wan
  • Patent number: 9890413
    Abstract: The invention related to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out this method. The basis of the invention is, in particular, a method for purification, stabilization or/and isolation of nucleic acids from material samples, whereby a buffer is added to the sample containing the nucleic acids, with a pH value of 2 to 7, a salt concentration of at least 100 mM, or/and a phenol neutralizing substance. According to the invention, pure nucleic acids which may be amplified can be obtained from faecal samples by a simple method, which are suitable for diagnostic proof of infection, in particular, bacterial or viral infection, or mutation, in particular, for tumor-specific DNA mutations.
    Type: Grant
    Filed: January 24, 2006
    Date of Patent: February 13, 2018
    Assignee: QIAGEN GMBH
    Inventor: Markus Sprenger-Haussels
  • Patent number: 9765108
    Abstract: The present invention relates to polymorphic forms of 5-Azacytidine and the process for preparation thereof. The present invention further relates to Crystalline 5-azacytidine 5 designated as Form-SA-1 characterized by an X-ray powder diffraction pattern having at least four characteristic diffraction angle peaks at about 12.00, 12.60, 13.90, 15.15 and 31.40±0.20 2?°, which is useful as active pharmaceutical ingredient in pharmaceutical compositions for the treatment of myelodysplastic syndrome.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: September 19, 2017
    Assignee: SHILPA MEDICARE LIMITED
    Inventors: Pradeep Shivakumar, Nagaraju Dasari, Ravi Kishore, Rizwan Ahmed, Akshaykant Chaturvedi
  • Patent number: 9708645
    Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support while maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.
    Type: Grant
    Filed: November 28, 2011
    Date of Patent: July 18, 2017
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Darren Ellis, Geir Fonnum, Nini Hofslokken Kjus
  • Patent number: 9683229
    Abstract: The present invention relates to matrix materials suitable for use in purifying and/or isolating nucleic acids from a biological sample, which matrix comprises a surface comprising at least one element selected from the group consisting of Germanium, Tin and/or Lead, or at least one salt thereof, and methods related therewith.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: June 20, 2017
    Inventor: Ralph Markus Wirtz
  • Patent number: 9513196
    Abstract: The present invention relates to methods and systems for microfluidic DNA sample preparation. More specifically, embodiments of the present invention relate to methods and systems for the isolation of DNA from patient samples on a microfluidic device and use of the DNA for downstream processing, such as performing amplification reactions and thermal melt analysis on the microfluidic device.
    Type: Grant
    Filed: November 8, 2012
    Date of Patent: December 6, 2016
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Weidong Cao, Hiroshi Inoue, Kevin Louder
  • Patent number: 9498737
    Abstract: [Object] To provide a method of purifying nucleic acids where the operation is simple and the nucleic acids can be extracted in a short time with high efficiency. [Solving Means] A method of purifying nucleic acids including the step of adsorbing substances in a sample containing nucleic acids with an ion exchange resin 10 including a positive ion exchange resin and a negative ion exchange resin. As the positive ion exchange resin, a first positive ion exchange resin and a second positive ion exchange resin having an exclusion limit molecular weight lower than that of the first positive ion exchange resin may be used.
    Type: Grant
    Filed: August 28, 2012
    Date of Patent: November 22, 2016
    Assignee: Sony Corporation
    Inventors: Tomohiko Nakamura, Naohisa Sakamoto, Tasuku Yotoriyama, Kazumine Ito
  • Patent number: 9493736
    Abstract: The present invention relates to a method for the lysis of cells, especially bacterial cells and the isolation of nucleic acids. The sample is treated with lysis solution that comprises at least one liquid that is not miscible with water and heated. Afterwards the mixture is cooled down and water is added so that after cooling a two phase system is generated. The nucleic acids can be found in the aqueous phase.
    Type: Grant
    Filed: March 28, 2012
    Date of Patent: November 15, 2016
    Assignee: MERCK PATENT GMBH
    Inventors: Joerg Slaghuis, Peter Rossmanith, Sabine Fuchs, Patrick Julian Mester, Martin Wagner
  • Patent number: 9487820
    Abstract: The invention discloses a method of extracting nucleic acid analyte solution from biological sample, comprising: placing the biological sample into a heating container; optionally, adding solvent medium into the biological sample; heating the biological sample under high pressure; optionally, centrifuging the biological sample; and obtaining the solution including the nucleic acid analyte. The invention also discloses a method of detecting the nucleic acid analyte obtained by said extraction method.
    Type: Grant
    Filed: April 27, 2011
    Date of Patent: November 8, 2016
    Assignee: Peking University
    Inventor: Haohao Zhong
  • Patent number: 9487550
    Abstract: The present invention pertains to a method of isolating RNA from a sample comprising RNA, and DNA, comprising: a) adding an acidic denaturing composition comprising a chaotropic agent and phenol to the sample; b) adding a water-insoluble organic solvent and separating the resulting phases thereby forming a multi-phase mixture comprising an aqueous phase, optionally an interphase and an organic phase, wherein the RNA is concentrated in said aqueous phase and DNA and proteins are concentrated in said organic phase and/or in said interphase; and c) isolating said RNA from said aqueous phase, wherein at least one cationic detergent is added before separating the phases. It was found that the addition of at least one cationic detergent considerably reduces the amount of DNA in the aqueous, RNA containing phase. Therefore, the present invention allows to easily isolate pure RNA which comprises considerably less DNA contaminations.
    Type: Grant
    Filed: September 6, 2011
    Date of Patent: November 8, 2016
    Assignee: QIAGEN GmbH
    Inventor: Gabriele Christoffel
  • Patent number: 9458452
    Abstract: The invention relates to a chromatographic device for isolating and/or purifying double-stranded nucleic acids, preferably double-stranded DNA, from a mixture of such nucleic acids with single-stranded nucleic acids, oligonucleotides, mononucleotides, salts and/or other such impurities. The invention also relates to a method for chromatographically isolating and/or purifying same, and to a kit for this purpose.
    Type: Grant
    Filed: November 8, 2011
    Date of Patent: October 4, 2016
    Assignee: QIAGEN GmbH
    Inventors: Thorsten Singer, Holger Wedler
  • Patent number: 9428544
    Abstract: It is an object of the present invention to provide a method for eluting an adsorbed protein that suppresses a decrease in protein adsorption ability, in a method for purifying a protein using a protein-adsorbing porous membrane. The present invention provides a method for purifying a protein, comprising an adsorption step and an elution step, wherein in the elution step, at least one eluent is passed in the opposite direction with respect to the direction of the passage of a stock solution containing an adsorption target protein, in the adsorption step.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: August 30, 2016
    Assignee: ASAHI KASEI CHEMICALS CORPORATION
    Inventors: Yuta Sato, Naoyuki Shinohara, Hironobu Shirataki
  • Patent number: 9416386
    Abstract: A cDNA synthesis method includes: mixing a lysis solution containing a chaotropic substance and a nucleic acid-binding solid-phase carrier in a sample containing a ribonucleic acid (RNA), thereby adsorbing the RNA on the carrier; reverse-transcribing the RNA adsorbed on the carrier while keeping the RNA adsorbed on the carrier in a reverse transcription reaction mixture, thereby synthesizing cDNA; and eluting the synthesized cDNA with an eluent.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: August 16, 2016
    Assignee: Seiko Epson Corporation
    Inventors: Yuji Saito, Fumio Takagi
  • Patent number: 9410193
    Abstract: The present invention is directed to methods to prepare a DNA molecule or a plurality of DNA molecules by random fragmentation. In some embodiments, the present invention regards preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3? end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, a library is prepared with methods of the present invention.
    Type: Grant
    Filed: August 4, 2014
    Date of Patent: August 9, 2016
    Assignee: Rubicon Genomics, Inc.
    Inventors: Vladimir L. Makarov, Irina Sleptsova, Emmanuel Kamberov, Eric Bruening
  • Patent number: 9334491
    Abstract: The present disclosure relates to systems and methods for nucleic acid isolation from cellular samples. In particular, the present disclosure provides systems and methods for lysing cells and recovering nucleic acids.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: May 10, 2016
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Thomas N. Chiesl, Michelle Toro, Steven Gerald Haupt
  • Patent number: 9220734
    Abstract: Composition based on polynucleotides extracted from natural sources for use in therapeutic treatment and/or as a therapeutic co-adjuvant in the treatment of degenerative diseases of the joints, in particular osteoarthritis.
    Type: Grant
    Filed: October 28, 2009
    Date of Patent: December 29, 2015
    Assignee: MASTELLI S.R.L.
    Inventors: Laura Cattarini Mastelli, Giulia Cattarini Mastelli
  • Patent number: 9222084
    Abstract: A method for i) parallel isolation of a double-stranded and/or a single-stranded nucleic acid and/or ii) selective removal of a double-stranded nucleic acid from a mixture of a double-stranded and a single-stranded nucleic acid or from a source comprising a double-stranded and a single-stranded nucleic acid includes absorbing the double-stranded nucleic acid onto a first solid carrier, while the single-stranded nucleic acid is not adsorbed and remains in solution, removing the first carrier with the adsorbed nucleic acid from the solution, mixing the solution comprising the single-stranded nucleic acid with an alcoholic solution having a concentration of 1 to 90 vol.-%, and contacting the resulting solution with second solid carrier, to absorb the single-stranded nucleic acid onto the second solid carrier.
    Type: Grant
    Filed: January 6, 2009
    Date of Patent: December 29, 2015
    Assignee: AJ Innuscreen GmbH
    Inventor: Timo Hillebrand
  • Patent number: 9217143
    Abstract: Disclosed are methods for isolating polynucleotides (e.g., RNA and DNA) from a cell-containing sample. The disclosed methods employ polyamidoamine (PAMAM) dendrimer molecules bound to the surface of binding particles to capture and isolate the polynucleotides from the samples. The polynucleotides are released from the binding particles by contacting the binding particles with a release solution having a basic pH.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: December 22, 2015
    Assignee: HANDYLAB, INC.
    Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
  • Patent number: 9133510
    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.
    Type: Grant
    Filed: October 15, 2013
    Date of Patent: September 15, 2015
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark Andersen, Steven Roman
  • Patent number: 9051563
    Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, the invention provides a method for nucleic acid purification from biological samples extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for differential isolation of RNA and DNA.
    Type: Grant
    Filed: January 12, 2012
    Date of Patent: June 9, 2015
    Assignee: Zymo Research Corporation
    Inventors: Stanislav Forman, Xiyu Jia
  • Publication number: 20150148255
    Abstract: This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a plurality of processing vessels and a collector. The collector funnels portions of the target material from the suspension through a cannula and into the processing vessels. Sequential density fractionation is the division of a sample into fractions or of a fraction of a sample into sub-fractions by a step-wise or sequential process, such that each step or sequence results in the collection or separation of a different fraction or sub-fraction from the preceding and successive steps or sequences. In other words, sequential density fractionation provides individual sub-populations of a population or individual sub-sub-populations of a sub-population of a population through a series of steps.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 28, 2015
    Applicant: RareCyte, Inc.
    Inventors: Lance U'ren, Daniel Campton, Joshua Nordberg, Steve Quarre, Jonathan Lundt
  • Patent number: 9040678
    Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.
    Type: Grant
    Filed: August 2, 2012
    Date of Patent: May 26, 2015
    Assignee: Illumina, Inc.
    Inventors: Frank J. Steemers, Kevin L. Gunderson, Kerri York, Ryan Christopher Smith
  • Publication number: 20150133649
    Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
    Type: Application
    Filed: November 7, 2014
    Publication date: May 14, 2015
    Inventors: Brian REED, James A. Ball
  • Publication number: 20150132758
    Abstract: Disclosed is a process for retrieval of nucleotide sequence. The process includes mixing iron chloride tetrahydrate with iron (III) chloride hexahydrate in solution; adding ammonium hydroxide to the mixture and stirring to form maghemite nanoparticles; stirring the maghemite nanoparticles in a solution with an inorganic acid, a surfactant and a monomer precursor of a conducting polymer; initiating polymerization of the monomer by adding the inorganic acid and an oxidizing agent to the stirred solution and further stirring to yield Polyaniline/maghemite nanocomposites; adding the nanocomposites to an first aqueous solution of the nucleotide sequence and stirring so as to electrostatically interact the nanocomposites with the nucleotide sequence; and weakening the electrostatic interaction between the nanocomposite and the nucleotide sequence to recover the nanocomposite independently of the nucleotide sequence.
    Type: Application
    Filed: January 12, 2015
    Publication date: May 14, 2015
    Inventors: Juan Carlos Medina-Llamas, Alicia Elizabeth Chávez-Guajardo, Cesar Augusto Souza de Andrade, Kleber Goncalves Bezerra Alves, Celso Pinto de Melo
  • Publication number: 20150133618
    Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2?CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
    Type: Application
    Filed: April 22, 2013
    Publication date: May 14, 2015
    Inventors: Jesper Hanssen, Gustav Rodrigo, Tobias E Soderman
  • Patent number: 9029529
    Abstract: Disclosed herein are processes for collecting nucleic acids from particulate samples. One embodiment disclosed herein relates to the use of ultrasonic energy to simultaneously shear large nucleic acid molecules and large particulates to very small sizes prior to or during a chemical binding step to a nucleic acid binding surface. Another embodiment involves crushing the nucleic acid binding surface prior to eluting the bound nucleic acid molecules to enable better wetting of the nucleic acid binding surface and easier diffusion of bound nucleic acid molecules out of the nucleic acid binding surface.
    Type: Grant
    Filed: March 14, 2012
    Date of Patent: May 12, 2015
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: T. Joseph Dennes, Michael P. Perry
  • Patent number: 9024008
    Abstract: Procedure for the specific isolation of total DNA content of bacterial germs of different samples, in the course of which the cells are lysated, the DNA content of the lysate is bound selectively, it is washed and then the desalinated linear polymer nucleic acid is eluted from the binding surface in an aqueous solution. Before cell lysis the nonviable bacterial cells are separated from the viable cells on the basis of their different cell surface physical-chemical characteristics, the viable cells of the sample are kept and then lysated using a mechanical and/or enzymatic, favorably lysozyme enzymatic method. After this exclusively double-stranded DNA deriving from the lysate of viable cells is bound on a —SiO2—TiO2- matrix containing chemically activated —OH and dodecylamine groups, and after washing it, the desalinated linear polymer nucleic acid is eluted in an aqueous solution.
    Type: Grant
    Filed: July 7, 2010
    Date of Patent: May 5, 2015
    Assignee: Diagon Ltd.
    Inventors: Gabor Kiss, Janos Kiss, Katalin Sztancsik Ambrusné Kovács, Georgina Bernath
  • Publication number: 20150119563
    Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.
    Type: Application
    Filed: December 29, 2014
    Publication date: April 30, 2015
    Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde
  • Publication number: 20150118684
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Application
    Filed: October 3, 2014
    Publication date: April 30, 2015
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Patent number: 9017621
    Abstract: Systems and methods for preparing a sample for further analysis are provided. The system can include an enclosure. A membrane can be disposed within the enclosure. First and second reservoirs can be disposed within the enclosure, and at least one of the first and second reservoirs can be adapted to have a reagent disposed therein. A valve can be disposed within the enclosure and in fluid communication with the first or second reservoirs or both. The valve can also be in fluid communication with the membrane. The valve can be adapted to selectively regulate the flow of the reagent from the first reservoir, through the membrane, and into the second reservoir.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: April 28, 2015
    Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space Administration
    Inventors: Ye Zhang, Honglu Wu
  • Patent number: 9012212
    Abstract: A method and system is provided for yielding biopharmaceutical products involving a chromatographic separation process. The method comprises: providing a plurality of membrane adsorber cartridges; providing a plurality of valves, communicatively coupled to said plurality of membrane adsorber cartridges; and switching the valves, so as to interconnect said membrane adsorber cartridges to operate in a countercurrent flow mode. The system comprises multiple membrane adsorber cartridges that are interconnected and configured to operate in a countercurrent flow mode. Furthermore, the configuration comprises a valve assembly that allows the cartridges to be subjected to different steps in the process by automatic switching of the valves. In this way, cartridges are recycled many times during the purification of a batch.
    Type: Grant
    Filed: April 17, 2007
    Date of Patent: April 21, 2015
    Assignee: Xendo Holding B.V.
    Inventors: Marc Antonius Theodorus Bisschops, Jozef Anton Mari Pennings, Jacob Arthur Tijsterman
  • Patent number: 9006419
    Abstract: A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.
    Type: Grant
    Filed: October 15, 2010
    Date of Patent: April 14, 2015
    Assignee: Industrial Technology Research Institute
    Inventors: Pei-Shin Jiang, Kun-Chan Wu, Yu-Ting Su, Chia-Yun Lin, Siou-Cing Su, Yuh-Jiuan Lin
  • Patent number: 9006420
    Abstract: A simple and convenient method for concentrating a biomolecule, including protein or nucleic acid molecules, from a sample. Purified and isolated biomolecules obtained by this method. Methods for improving the specificity or sensitivity of detecting a biomolecule by concentration and/or purification or isolation of the biomolecule according to the method of the invention.
    Type: Grant
    Filed: November 8, 2010
    Date of Patent: April 14, 2015
    Inventor: Timo Hillebrand
  • Patent number: 9000146
    Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting multiple DNA targets from a human stool sample with, for example, methods employing spin filtration techniques.
    Type: Grant
    Filed: May 11, 2012
    Date of Patent: April 7, 2015
    Assignee: Exact Sciences Corporation
    Inventors: Janelle J. Bruinsma, Michael J. Domanico, Graham P. Lidgard, Hongzhi Zou, William G. Weisburg, Hemanth D. Shenoi, James P. Light, II
  • Publication number: 20150093750
    Abstract: The invention relates to a processing device (100) and a method for processing a medium in a processing chamber (121). The processing comprises the addition of magnetic particles (M) to the medium and the mixing of the medium by manipulating said magnetic particles with a time-variable magnetic field (B), particularly a partially oscillating or rotating field. The magnetic field (B) may be generated with a multipole magnetic field generator (110) comprising four subunits (111A,111B), each having a core (113A,113B) with a surrounding coil (112A,112B) and with a top surface (114A,114B), wherein all top surfaces of said subunits are preferably arranged in the same plane and wherein all cores are substantially parallel to each other.
    Type: Application
    Filed: April 23, 2013
    Publication date: April 2, 2015
    Inventors: Mikhail Mikhaylovich Ovsyanko, Pieter Jan Van Der Zaag, Harma Martine Feitsma, Reinhold Wimberger-Friedl, Theodorus Antonius Johannes Löring, Martinus Johannes Van Zelst
  • Patent number: 8980552
    Abstract: The invention describes a method of and kits for isolating and/or purifying nucleic acids, more specifically short-chain nucleic acids such as miRNA, from a nucleic acid-containing starting material, characterized by the following method steps of: (a) binding the nucleic acids to a nucleic acid-binding support material by contacting the starting material with said nucleic acid-binding support material in the presence of at least one chaotropic compound, at least two different detergents and at least one branched and/or unbranched alcohol, preferably isopropanol, with the concentration of said alcohol being 40% (v/v); (b) optionally eluting the bound nucleic acids from the nucleic acid-binding support material. The method of the invention is particularly suitable for purifying circulating, extracellular miRNA from blood.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: March 17, 2015
    Assignee: QIAGEN GmbH
    Inventors: Martin Horlitz, Markus Sprenger-Haussels
  • Patent number: 8980553
    Abstract: Compositions and methods are provided for enriching non-target polynucleotides from a mixture of non-target and target polynucleotides where differences between the target polynucleotides and the non-target polynucleotides include the extent of modified bases that are present in a greater density in the target polynucleotides than in the non-target polynucleotides. This permits the target polynucleotides to be selectively and rapidly bound to an affinity matrix such as affinity protein-coated magnetic beads providing enrichment of the non-target polynucleotides in the supernatant. One use of this enrichment is to remove human genomic DNA from a mixture of DNAs obtained from human tissue samples to enrich for polynucleotides in a microbiome so as to characterize the microbiome by DNA sequencing.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: March 17, 2015
    Assignee: New England Biolabs, Inc.
    Inventors: George R. Feehery, Fiona Stewart, James McFarland, Sriharsa Pradhan
  • Publication number: 20150064764
    Abstract: A device for transporting, trapping and escaping a single biomaterial using a magnetic structure, and a method of transporting, trapping and escaping of the single biomaterial using the same are provided, and a method is provided for controlling movement and direction of the single biomaterial including soft magnetic micro structure and magnetic structure in a linear, square storage, apartment type, radial soft magnetic micro structure. Accordingly, the device for transporting, trapping and escaping a single biomaterial and the method for transporting, trapping and escaping single biomaterial using the same can control movement on the lap-on-a-chip with increased precision and ease, by using magnetic force, and thus can be advantageously used in the field of magneto-resistive sensor, or categorization of single cells or biomolecules.
    Type: Application
    Filed: August 29, 2013
    Publication date: March 5, 2015
    Applicant: The Industry & Academic Cooperation in Chungnam National University (IAC)
    Inventors: Cheolgi Kim, Byeonghwa Lim, Reddy Venu, Hu XingHao, KunWoo Kim, Benjamin B. Yellen
  • Patent number: 8969545
    Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.
    Type: Grant
    Filed: October 18, 2012
    Date of Patent: March 3, 2015
    Assignee: Life Technologies Corporation
    Inventors: Anilkumar R. Kore, Shanmugasundaram Muthian, Kyle Gee
  • Publication number: 20150057377
    Abstract: A method for breaking emulsions includes applying a polymer mixture to an emulsion. The emulsion can be energized, such as through centrifugation or vibration. In particular, the polymer mixture can be in liquid form. The polymer mixture includes first and second liquid polymer, the second liquid polymer being less hydrophilic than the first liquid polymer. In a water-in-oil emulsion, the less hydrophilic polymer can preferentially reside within the oil phase.
    Type: Application
    Filed: October 30, 2014
    Publication date: February 26, 2015
    Inventors: Ryan JONES, Maximilian CARPINO
  • Publication number: 20150050727
    Abstract: The invention provides methods of purifying one or more biological analytes from a sample. The method comprises incubating the sample with nanoparticles and centrifuging a biphasic system. The biological analytes can be one or more of an enzyme, a protein, a nucleic acid, an organelle, a cell, a bacteria, a virus, or any other biological material which requires purification.
    Type: Application
    Filed: August 13, 2014
    Publication date: February 19, 2015
    Inventors: Joshua Benno Edel, Vladimir Alexander Turek, Anthony Edward George Cass