Separation Or Purification Of Polynucleotides Or Oligonucleotides Patents (Class 536/25.4)
  • Patent number: 10801042
    Abstract: The present invention is directed to methods for increasing the efficiencies with which recombinant adeno-associated virus (rAAV) are packaged, so as to increase their production titers. More specifically, the invention relates to a method for increasing the production titer of rAAV by transfected cells by increasing the ionic strength of the cell culture media through the administration of additional ions.
    Type: Grant
    Filed: July 15, 2019
    Date of Patent: October 13, 2020
    Assignee: Vigene Biosciences, Inc.
    Inventor: Qizhao Wang
  • Patent number: 10761000
    Abstract: In a liquid stirring method, after a second liquid is discharged into a reaction container accommodating a first liquid from a dispensing probe provided with a dispensing tip at the leading end thereof, a mixture of the first liquid and second liquid in the container is stirred by being sucked out and discharged by the dispensing probe. The number of stirrings through sucking out and discharging is changed according to the total volume of the first liquid and second liquid. If the total volume of the first liquid and second liquid is below a preset threshold, sucking out and discharging is repeated for a prescribed number of times.
    Type: Grant
    Filed: June 15, 2015
    Date of Patent: September 1, 2020
    Assignee: HITACHI HIGH-TECH CORPORATION
    Inventors: Shunsuke Sasaki, Yoshihiro Yamashita
  • Patent number: 10744504
    Abstract: A microscale sampling device including a frame is provided in the present invention, a sample container, a communicating channel and a resistance channel are defined in the frame. At least one sampling chamber is defined in the communicating channel. An end of the communicating channel is communicated with the sample container and the communicating channel is arranged below the sample container. An end of the resistance channel is communicated with the sampling chamber, and the other end of the resistance channel is communicated to an output joint. The resistance channel is shaped with at least one discontinuous shape change.
    Type: Grant
    Filed: December 29, 2017
    Date of Patent: August 18, 2020
    Assignee: DELTA ELECTRONICS, INC.
    Inventors: Song-Bin Huang, Wei-Yu Chung, Shing-Lun Liu, Qian Liang, Yu-Kai Kao
  • Patent number: 10745687
    Abstract: Aspects of the disclosure include methods for the preparation of a polynucleotide. In some embodiments, the method includes contacting a first polynucleotide composition including: a polynucleotide having a sequence of 7 or more nucleoside subunits and at least two of the nucleoside subunits are joined by a N3??P5? thiophosphoramidate inter-subunit linkage; and non-target synthetic products and reagents; with a multivalent cation salt to precipitate a polynucleotide salt including at least one multivalent cation counterion; and separating the polynucleotide salt from the contacted first polynucleotide composition to produce a second polynucleotide composition including the polynucleotide salt. In certain embodiments, the method further includes contacting the polynucleotide salt with a reverse phase chromatography support; and eluting from the chromatography support a third polynucleotide composition including the polynucleotide.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: August 18, 2020
    Assignee: Geron Corporation
    Inventor: Premchandran H. Ramiya
  • Patent number: 10655122
    Abstract: The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.
    Type: Grant
    Filed: April 20, 2015
    Date of Patent: May 19, 2020
    Assignee: QIAGEN GMBH
    Inventors: Johann Kubicek, Thorsten Singer, Antje-Katrin Sander, Eva Hänssler, Dominic O'Neil
  • Patent number: 10590410
    Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples are provided. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations. The binding particles can be coated with PAMAM (Generation 0) dendrimer which is amide-bonded to the binding particles, wherein at least a portion of the nucleic acids in the biological sample become reversibly bonded to the binding particles.
    Type: Grant
    Filed: October 12, 2018
    Date of Patent: March 17, 2020
    Assignee: HANDYLAB, INC.
    Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
  • Patent number: 10576396
    Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: March 3, 2020
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Brian Reed, James A. Ball
  • Patent number: 10557853
    Abstract: The present invention is directed to fluorescent molecular sensor based on Thiazole Orange for protein detection. Interaction of the protein target with the molecular sensors of this invention results in a significant increase in the fluorescence emission. The generation of light output signal enables one to detect protein biomarkers associated with different diseases or detecting the protein of interest also in living cells.
    Type: Grant
    Filed: July 2, 2017
    Date of Patent: February 11, 2020
    Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.
    Inventors: David Margulies, Leila Motiei, Linor Unger
  • Patent number: 10537106
    Abstract: A gold nanoparticle-based assay for the detection of a target molecule, such as Hepatitis C Virus (HCV) RNA in serum samples, that uses positively charged gold nanoparticles (AuNPs) in solution based format. The assay has been tested on 74 serum clinical samples suspected of containing HCV RNA, with 48 and 38 positive and negative samples respectively. The developed assay has a specificity and sensitivity of 96.5% and 92.6% respectively. The results obtained were confirmed by Real-Time PCR, and a concordance of 100% for the negative samples and 89% for the positive samples has been obtained between the Real-Time PCR and the developed AuNPs based assay. Also, a purification method for the HCV RNA has been developed using HCV RNA specific probe conjugated to homemade silica nanoparticles. These silica nanoparticles have been synthesized by modified Stober method. This purification method enhanced the specificity of the developed AuNPs assay.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: January 21, 2020
    Assignee: AMERICAN UNIVERSITY IN CAIRO (AUC)
    Inventors: Hassan Mohamed El-Said Azzazy, Sherif Mohamed Shawky Abduo, Kamel Abdelmenem Mohamed Eid, Bassem Samy Shenouda Guirgis
  • Patent number: 10494663
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Grant
    Filed: August 29, 2019
    Date of Patent: December 3, 2019
    Assignee: HandyLab, Inc.
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Patent number: 10457931
    Abstract: Disclosed is a process for purifying nucleic acids, especially plasmid DNA, from a nucleic acid-containing, biologic sample, consisting of (a) preparation of a fluid sample containing nucleic acid and endotoxin each in dissolved form; (b) precipitation at least of nucleic acid and endotoxin from the fluid sample; (c) washing of the components of the fluid sample precipitated out in step b) in order to at least partially remove the endotoxin with at least one washing solution, which contains at least one amine compound with at least two carbon atoms and with a molar mass of ?500 g/mol and; at least one organic solvent different from the aforementioned amine compound and has a pH-value (20° C.) in the range from pH 3.0 to pH 8.5, and; (d) dissolution of the remaining nucleic acid from the washed, precipitated constituents from step c) using a dissolving buffer and collection of the dissolved nucleic acids in a separate receptacle. The process is suitable for the effective and economical removal of endotoxins.
    Type: Grant
    Filed: April 3, 2017
    Date of Patent: October 29, 2019
    Assignee: AXAGARIUS GMBH & CO. KG
    Inventors: Stefan Heymans, Thorsten Rosenbaum
  • Patent number: 10450558
    Abstract: The present invention relates to a new method for enriching and/or isolating nucleic acids from microbial cells which comprises filtering a liquid sample through a nucleic acid-binding matrix which has a pore size small enough to retain microbial cells, lysing the microbial cells on the matrix to release the nucleic acids from the microbial cells, binding the nucleic acids to the matrix and subsequently eluting the DNA. The invention also relates to a method for enriching and/or isolating nucleic acids from microbial cells which are present in a liquid sample that comprises microbial cells and higher eukaryotic cells and/or tissues. The invention also provides a cartridge for carrying out the methods of the invention. Finally, the invention relates to kits for carrying out the methods of the invention.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: October 22, 2019
    Assignee: Molzym GmbH & Co. KG
    Inventor: Michael Lorenz
  • Patent number: 10407713
    Abstract: The present invention provides a special reagent for the pretreatment of sample materials. The pretreatment with the reagent according to the invention enables the isolation of nucleic acids by a uniform method from very diverse sample materials, in particular different bioprocess samples, even if the nucleic acids are only present in small amounts in the sample materials. The reagent used for the pretreatment comprises, as key component, at least one compound comprising an amino group. The invention further relates to methods of purification of nucleic acids, in which the sample material is pretreated correspondingly, and suitable kits.
    Type: Grant
    Filed: July 4, 2012
    Date of Patent: September 10, 2019
    Assignee: QIAGEN GmbH
    Inventor: Peter Porschewski
  • Patent number: 10227582
    Abstract: The present disclosure relates to systems and methods for nucleic acid isolation from cellular samples. In particular, the present disclosure provides systems and methods for lysing cells and recovering nucleic acids.
    Type: Grant
    Filed: May 9, 2016
    Date of Patent: March 12, 2019
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Thomas N. Chiesl, Michelle Toro, Steven Gerald Haupt
  • Patent number: 10201620
    Abstract: The present invention relates to compositions, kits and methods for making and using RNA compositions comprising in vitro-synthesized ssRNA inducing a biological or biochemical effect in a mammalian cell or organism into which the RNA composition is repeatedly or continuously introduced. In certain embodiments, the invention provides compositions and methods for changing the state of differentiation or phenotype of a human or other vertebrate cell. For example, the present invention provides mRNA and methods for reprogramming cells that exhibit a first differentiated state or phenotype to cells that exhibit a second differentiated state or phenotype, such as to reprogram human somatic cells to pluripotent stem cells.
    Type: Grant
    Filed: December 31, 2012
    Date of Patent: February 12, 2019
    Assignee: CELLSCRIPT, LLC
    Inventors: Judith Meis, Anthony Person, Cynthia Chin, Jerome Jendrisak, Gary Dahl
  • Patent number: 10184145
    Abstract: The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps: binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture; separating the solid phase with the bound extracellular nucleic acids; optionally washing the extracellular nucleic acids; optionally eluting extracellular nucleic acids from the solid phase. The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.
    Type: Grant
    Filed: September 25, 2012
    Date of Patent: January 22, 2019
    Assignee: QIAGEN GmbH
    Inventors: Martin Horlitz, Annette Nocon, Markus Sprenger-Haussels, Peter Grünefeld, Christoph Erbacher
  • Patent number: 10131935
    Abstract: Parallel isolation of a double-stranded nucleic acid and a single-stranded nucleic acid is possible from a sample that contains these acids, without separating the acids, by mixing the sample with a lysis buffer having high salt concentration or low salt concentration, or having a proteolytic enzyme. The sample that contains nucleic acid before its lysis, or the sample that has already been lysed or homogenized, is adjusted with a binding buffer in such a manner that the total nucleic acid is adsorbed onto a solid carrier. The binding buffer contains at least one non-ionic detergent in a high concentration. With the exception of the detergent, the sample contains no other non-acidic organic component miscible in water. The carrier with the adsorbed total nucleic acid is removed. The adsorbed total nucleic acid is washed and eluted.
    Type: Grant
    Filed: January 12, 2009
    Date of Patent: November 20, 2018
    Assignee: AJ INNUSCREEN GmbH
    Inventor: Timo Hillebrand
  • Patent number: 10087439
    Abstract: The invention is directed to compositions and methods for rapidly and efficiently extracting nucleic acids and/or targeted nucleic acids sequences from biological samples. The methods of the invention comprise combining the sample with a buffer and magnetic silicon beads and concentrating the beads with a magnet or other electrical field. Liquid may be removed, or not, and an alkaline buffer is added followed by magnetic carboxy beads in a binding buffer so that nucleic acids transfer to the carboxy beads, which can be easily and quickly isolated once again with a magnet. Total nucleic acid extraction is greatly enhanced. Extracted nucleic acids can be analyzed, for example, by PCR wherein the nucleic acids can be identified and characterized. Carboxy beads may also contain a ligand so as to target specific nucleic acid sequences. The invention is also directed to kits comprising the tools and compositions for performing the methods of the invention.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: October 2, 2018
    Assignee: Longhorn Vaccines and Diagnostics, LLC
    Inventors: Gerald W. Fischer, Luke T. Daum
  • Patent number: 9976136
    Abstract: The invention is directed to compositions and methods for rapidly and efficiently extracting nucleic acids and/or targeted nucleic acids sequences from biological samples. The methods of the invention comprise combining the sample with a buffer and magnetic silicon beads and concentrating the beads with a magnet or other electrical field. Liquid may be removed, or not, and an alkaline buffer is added followed by magnetic carboxy beads in a binding buffer so that nucleic acids transfer to the carboxy beads, which can be easily and quickly isolated once again with a magnet. Total nucleic acid extraction is greatly enhanced. Extracted nucleic acids can be analyzed, for example, by PCR wherein the nucleic acids can be identified and characterized. Carboxy beads may also contain a ligand so as to target specific nucleic acid sequences. The invention is also directed to kits comprising the tools and compositions for performing the methods of the invention.
    Type: Grant
    Filed: May 12, 2016
    Date of Patent: May 22, 2018
    Assignee: Longhorn Vaccines and Diagnostics, LLC
    Inventors: Gerald W. Fischer, Luke T. Daum
  • Patent number: 9976993
    Abstract: An apparatus for extracting an analyte from a liquid sample having a container with an entrance, an exit, and a passage therebetween for passage of a liquid sample containing an analyte, the container having a full diameter bed region and a reduced diameter bed region. The container includes a layered construction extending across the passage, having from top to bottom one or more of: (i) an upper flow distributor/support layer, (ii) an upper compression layer, (iii) an extraction layer of microparticulate extraction medium adjacent to the layer (ii), and (iv) a lower compression layer located adjacent to the extraction layer (iii), optionally including one or more air gap layers. At least some of the layers are located in the full diameter bed region, and some of the layers are located in the reduced diameter bed region. The apparatus may have a plurality of containers arranged in an array and/or in series so as to provide multi-stage filtration or extraction.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: May 22, 2018
    Assignee: TECAN SP, INC.
    Inventor: Philip A. Dimson
  • Patent number: 9907702
    Abstract: Wound dressing articles comprising a nonwoven web comprising a plurality of fibers having grafted pendant hydrophilic groups, methods that use high energy irradiation for making a plurality of fibers having grafted pendant hydrophilic groups, useful for making wound dressing articles.
    Type: Grant
    Filed: August 13, 2012
    Date of Patent: March 6, 2018
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Cary A. Kipke, John J. Rogers, Michael R. Berrigan, Clinton P. Waller, Jr., Douglas E. Weiss
  • Patent number: 9896682
    Abstract: Methods and compositions are described herein for protecting RNA from autocatalytic and divalent cation induced degradation in an aqueous solution.
    Type: Grant
    Filed: March 7, 2016
    Date of Patent: February 20, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Xiao-Song Gong, Cindy Wan
  • Patent number: 9890413
    Abstract: The invention related to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out this method. The basis of the invention is, in particular, a method for purification, stabilization or/and isolation of nucleic acids from material samples, whereby a buffer is added to the sample containing the nucleic acids, with a pH value of 2 to 7, a salt concentration of at least 100 mM, or/and a phenol neutralizing substance. According to the invention, pure nucleic acids which may be amplified can be obtained from faecal samples by a simple method, which are suitable for diagnostic proof of infection, in particular, bacterial or viral infection, or mutation, in particular, for tumor-specific DNA mutations.
    Type: Grant
    Filed: January 24, 2006
    Date of Patent: February 13, 2018
    Assignee: QIAGEN GMBH
    Inventor: Markus Sprenger-Haussels
  • Patent number: 9765108
    Abstract: The present invention relates to polymorphic forms of 5-Azacytidine and the process for preparation thereof. The present invention further relates to Crystalline 5-azacytidine 5 designated as Form-SA-1 characterized by an X-ray powder diffraction pattern having at least four characteristic diffraction angle peaks at about 12.00, 12.60, 13.90, 15.15 and 31.40±0.20 2?°, which is useful as active pharmaceutical ingredient in pharmaceutical compositions for the treatment of myelodysplastic syndrome.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: September 19, 2017
    Assignee: SHILPA MEDICARE LIMITED
    Inventors: Pradeep Shivakumar, Nagaraju Dasari, Ravi Kishore, Rizwan Ahmed, Akshaykant Chaturvedi
  • Patent number: 9708645
    Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support while maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.
    Type: Grant
    Filed: November 28, 2011
    Date of Patent: July 18, 2017
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Darren Ellis, Geir Fonnum, Nini Hofslokken Kjus
  • Patent number: 9683229
    Abstract: The present invention relates to matrix materials suitable for use in purifying and/or isolating nucleic acids from a biological sample, which matrix comprises a surface comprising at least one element selected from the group consisting of Germanium, Tin and/or Lead, or at least one salt thereof, and methods related therewith.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: June 20, 2017
    Inventor: Ralph Markus Wirtz
  • Patent number: 9513196
    Abstract: The present invention relates to methods and systems for microfluidic DNA sample preparation. More specifically, embodiments of the present invention relate to methods and systems for the isolation of DNA from patient samples on a microfluidic device and use of the DNA for downstream processing, such as performing amplification reactions and thermal melt analysis on the microfluidic device.
    Type: Grant
    Filed: November 8, 2012
    Date of Patent: December 6, 2016
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Weidong Cao, Hiroshi Inoue, Kevin Louder
  • Patent number: 9498737
    Abstract: [Object] To provide a method of purifying nucleic acids where the operation is simple and the nucleic acids can be extracted in a short time with high efficiency. [Solving Means] A method of purifying nucleic acids including the step of adsorbing substances in a sample containing nucleic acids with an ion exchange resin 10 including a positive ion exchange resin and a negative ion exchange resin. As the positive ion exchange resin, a first positive ion exchange resin and a second positive ion exchange resin having an exclusion limit molecular weight lower than that of the first positive ion exchange resin may be used.
    Type: Grant
    Filed: August 28, 2012
    Date of Patent: November 22, 2016
    Assignee: Sony Corporation
    Inventors: Tomohiko Nakamura, Naohisa Sakamoto, Tasuku Yotoriyama, Kazumine Ito
  • Patent number: 9493736
    Abstract: The present invention relates to a method for the lysis of cells, especially bacterial cells and the isolation of nucleic acids. The sample is treated with lysis solution that comprises at least one liquid that is not miscible with water and heated. Afterwards the mixture is cooled down and water is added so that after cooling a two phase system is generated. The nucleic acids can be found in the aqueous phase.
    Type: Grant
    Filed: March 28, 2012
    Date of Patent: November 15, 2016
    Assignee: MERCK PATENT GMBH
    Inventors: Joerg Slaghuis, Peter Rossmanith, Sabine Fuchs, Patrick Julian Mester, Martin Wagner
  • Patent number: 9487820
    Abstract: The invention discloses a method of extracting nucleic acid analyte solution from biological sample, comprising: placing the biological sample into a heating container; optionally, adding solvent medium into the biological sample; heating the biological sample under high pressure; optionally, centrifuging the biological sample; and obtaining the solution including the nucleic acid analyte. The invention also discloses a method of detecting the nucleic acid analyte obtained by said extraction method.
    Type: Grant
    Filed: April 27, 2011
    Date of Patent: November 8, 2016
    Assignee: Peking University
    Inventor: Haohao Zhong
  • Patent number: 9487550
    Abstract: The present invention pertains to a method of isolating RNA from a sample comprising RNA, and DNA, comprising: a) adding an acidic denaturing composition comprising a chaotropic agent and phenol to the sample; b) adding a water-insoluble organic solvent and separating the resulting phases thereby forming a multi-phase mixture comprising an aqueous phase, optionally an interphase and an organic phase, wherein the RNA is concentrated in said aqueous phase and DNA and proteins are concentrated in said organic phase and/or in said interphase; and c) isolating said RNA from said aqueous phase, wherein at least one cationic detergent is added before separating the phases. It was found that the addition of at least one cationic detergent considerably reduces the amount of DNA in the aqueous, RNA containing phase. Therefore, the present invention allows to easily isolate pure RNA which comprises considerably less DNA contaminations.
    Type: Grant
    Filed: September 6, 2011
    Date of Patent: November 8, 2016
    Assignee: QIAGEN GmbH
    Inventor: Gabriele Christoffel
  • Patent number: 9458452
    Abstract: The invention relates to a chromatographic device for isolating and/or purifying double-stranded nucleic acids, preferably double-stranded DNA, from a mixture of such nucleic acids with single-stranded nucleic acids, oligonucleotides, mononucleotides, salts and/or other such impurities. The invention also relates to a method for chromatographically isolating and/or purifying same, and to a kit for this purpose.
    Type: Grant
    Filed: November 8, 2011
    Date of Patent: October 4, 2016
    Assignee: QIAGEN GmbH
    Inventors: Thorsten Singer, Holger Wedler
  • Patent number: 9428544
    Abstract: It is an object of the present invention to provide a method for eluting an adsorbed protein that suppresses a decrease in protein adsorption ability, in a method for purifying a protein using a protein-adsorbing porous membrane. The present invention provides a method for purifying a protein, comprising an adsorption step and an elution step, wherein in the elution step, at least one eluent is passed in the opposite direction with respect to the direction of the passage of a stock solution containing an adsorption target protein, in the adsorption step.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: August 30, 2016
    Assignee: ASAHI KASEI CHEMICALS CORPORATION
    Inventors: Yuta Sato, Naoyuki Shinohara, Hironobu Shirataki
  • Patent number: 9416386
    Abstract: A cDNA synthesis method includes: mixing a lysis solution containing a chaotropic substance and a nucleic acid-binding solid-phase carrier in a sample containing a ribonucleic acid (RNA), thereby adsorbing the RNA on the carrier; reverse-transcribing the RNA adsorbed on the carrier while keeping the RNA adsorbed on the carrier in a reverse transcription reaction mixture, thereby synthesizing cDNA; and eluting the synthesized cDNA with an eluent.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: August 16, 2016
    Assignee: Seiko Epson Corporation
    Inventors: Yuji Saito, Fumio Takagi
  • Patent number: 9410193
    Abstract: The present invention is directed to methods to prepare a DNA molecule or a plurality of DNA molecules by random fragmentation. In some embodiments, the present invention regards preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3? end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, a library is prepared with methods of the present invention.
    Type: Grant
    Filed: August 4, 2014
    Date of Patent: August 9, 2016
    Assignee: Rubicon Genomics, Inc.
    Inventors: Vladimir L. Makarov, Irina Sleptsova, Emmanuel Kamberov, Eric Bruening
  • Patent number: 9334491
    Abstract: The present disclosure relates to systems and methods for nucleic acid isolation from cellular samples. In particular, the present disclosure provides systems and methods for lysing cells and recovering nucleic acids.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: May 10, 2016
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Thomas N. Chiesl, Michelle Toro, Steven Gerald Haupt
  • Patent number: 9220734
    Abstract: Composition based on polynucleotides extracted from natural sources for use in therapeutic treatment and/or as a therapeutic co-adjuvant in the treatment of degenerative diseases of the joints, in particular osteoarthritis.
    Type: Grant
    Filed: October 28, 2009
    Date of Patent: December 29, 2015
    Assignee: MASTELLI S.R.L.
    Inventors: Laura Cattarini Mastelli, Giulia Cattarini Mastelli
  • Patent number: 9222084
    Abstract: A method for i) parallel isolation of a double-stranded and/or a single-stranded nucleic acid and/or ii) selective removal of a double-stranded nucleic acid from a mixture of a double-stranded and a single-stranded nucleic acid or from a source comprising a double-stranded and a single-stranded nucleic acid includes absorbing the double-stranded nucleic acid onto a first solid carrier, while the single-stranded nucleic acid is not adsorbed and remains in solution, removing the first carrier with the adsorbed nucleic acid from the solution, mixing the solution comprising the single-stranded nucleic acid with an alcoholic solution having a concentration of 1 to 90 vol.-%, and contacting the resulting solution with second solid carrier, to absorb the single-stranded nucleic acid onto the second solid carrier.
    Type: Grant
    Filed: January 6, 2009
    Date of Patent: December 29, 2015
    Assignee: AJ Innuscreen GmbH
    Inventor: Timo Hillebrand
  • Patent number: 9217143
    Abstract: Disclosed are methods for isolating polynucleotides (e.g., RNA and DNA) from a cell-containing sample. The disclosed methods employ polyamidoamine (PAMAM) dendrimer molecules bound to the surface of binding particles to capture and isolate the polynucleotides from the samples. The polynucleotides are released from the binding particles by contacting the binding particles with a release solution having a basic pH.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: December 22, 2015
    Assignee: HANDYLAB, INC.
    Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
  • Patent number: 9133510
    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.
    Type: Grant
    Filed: October 15, 2013
    Date of Patent: September 15, 2015
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark Andersen, Steven Roman
  • Patent number: 9051563
    Abstract: Methods and composition for nucleic acid isolation are provided. In one embodiment, the invention provides a method for nucleic acid purification from biological samples extracted with phenol-based denaturing solvents, which does not require phase separation or nucleic acid precipitation. Methods according to the invention may also be used for differential isolation of RNA and DNA.
    Type: Grant
    Filed: January 12, 2012
    Date of Patent: June 9, 2015
    Assignee: Zymo Research Corporation
    Inventors: Stanislav Forman, Xiyu Jia
  • Publication number: 20150148255
    Abstract: This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a plurality of processing vessels and a collector. The collector funnels portions of the target material from the suspension through a cannula and into the processing vessels. Sequential density fractionation is the division of a sample into fractions or of a fraction of a sample into sub-fractions by a step-wise or sequential process, such that each step or sequence results in the collection or separation of a different fraction or sub-fraction from the preceding and successive steps or sequences. In other words, sequential density fractionation provides individual sub-populations of a population or individual sub-sub-populations of a sub-population of a population through a series of steps.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 28, 2015
    Applicant: RareCyte, Inc.
    Inventors: Lance U'ren, Daniel Campton, Joshua Nordberg, Steve Quarre, Jonathan Lundt
  • Patent number: 9040678
    Abstract: Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags.
    Type: Grant
    Filed: August 2, 2012
    Date of Patent: May 26, 2015
    Assignee: Illumina, Inc.
    Inventors: Frank J. Steemers, Kevin L. Gunderson, Kerri York, Ryan Christopher Smith
  • Publication number: 20150133649
    Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
    Type: Application
    Filed: November 7, 2014
    Publication date: May 14, 2015
    Inventors: Brian REED, James A. Ball
  • Publication number: 20150133618
    Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2?CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
    Type: Application
    Filed: April 22, 2013
    Publication date: May 14, 2015
    Inventors: Jesper Hanssen, Gustav Rodrigo, Tobias E Soderman
  • Publication number: 20150132758
    Abstract: Disclosed is a process for retrieval of nucleotide sequence. The process includes mixing iron chloride tetrahydrate with iron (III) chloride hexahydrate in solution; adding ammonium hydroxide to the mixture and stirring to form maghemite nanoparticles; stirring the maghemite nanoparticles in a solution with an inorganic acid, a surfactant and a monomer precursor of a conducting polymer; initiating polymerization of the monomer by adding the inorganic acid and an oxidizing agent to the stirred solution and further stirring to yield Polyaniline/maghemite nanocomposites; adding the nanocomposites to an first aqueous solution of the nucleotide sequence and stirring so as to electrostatically interact the nanocomposites with the nucleotide sequence; and weakening the electrostatic interaction between the nanocomposite and the nucleotide sequence to recover the nanocomposite independently of the nucleotide sequence.
    Type: Application
    Filed: January 12, 2015
    Publication date: May 14, 2015
    Inventors: Juan Carlos Medina-Llamas, Alicia Elizabeth Chávez-Guajardo, Cesar Augusto Souza de Andrade, Kleber Goncalves Bezerra Alves, Celso Pinto de Melo
  • Patent number: 9029529
    Abstract: Disclosed herein are processes for collecting nucleic acids from particulate samples. One embodiment disclosed herein relates to the use of ultrasonic energy to simultaneously shear large nucleic acid molecules and large particulates to very small sizes prior to or during a chemical binding step to a nucleic acid binding surface. Another embodiment involves crushing the nucleic acid binding surface prior to eluting the bound nucleic acid molecules to enable better wetting of the nucleic acid binding surface and easier diffusion of bound nucleic acid molecules out of the nucleic acid binding surface.
    Type: Grant
    Filed: March 14, 2012
    Date of Patent: May 12, 2015
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: T. Joseph Dennes, Michael P. Perry
  • Patent number: 9024008
    Abstract: Procedure for the specific isolation of total DNA content of bacterial germs of different samples, in the course of which the cells are lysated, the DNA content of the lysate is bound selectively, it is washed and then the desalinated linear polymer nucleic acid is eluted from the binding surface in an aqueous solution. Before cell lysis the nonviable bacterial cells are separated from the viable cells on the basis of their different cell surface physical-chemical characteristics, the viable cells of the sample are kept and then lysated using a mechanical and/or enzymatic, favorably lysozyme enzymatic method. After this exclusively double-stranded DNA deriving from the lysate of viable cells is bound on a —SiO2—TiO2- matrix containing chemically activated —OH and dodecylamine groups, and after washing it, the desalinated linear polymer nucleic acid is eluted in an aqueous solution.
    Type: Grant
    Filed: July 7, 2010
    Date of Patent: May 5, 2015
    Assignee: Diagon Ltd.
    Inventors: Gabor Kiss, Janos Kiss, Katalin Sztancsik Ambrusné Kovács, Georgina Bernath
  • Publication number: 20150118684
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Application
    Filed: October 3, 2014
    Publication date: April 30, 2015
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Publication number: 20150119563
    Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.
    Type: Application
    Filed: December 29, 2014
    Publication date: April 30, 2015
    Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde