Abstract: The disclosure relates to metal organic frameworks or isoreticular metal organic frameworks, methods of production thereof, and methods of use thereof.
Type:
Application
Filed:
October 12, 2012
Publication date:
April 18, 2013
Applicant:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Abstract: It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.
Abstract: Methods are provided for purifying non-chromosomal nucleic acid molecules from cells, comprising: the general steps of (a) lysing cells to form a nucleic acid-containing lysate, and (b) applying the lysate to a depth filter in order to obtain a clarified solution containing purified non-chromosomal nucleic acid molecules.
Type:
Grant
Filed:
July 29, 1999
Date of Patent:
July 31, 2001
Assignee:
Chiron Corporation
Inventors:
Denice M. Mittelstaedt, David Chi-Tang Hsu