Oxygen Double Bonded And Nitrogen Bonded Directly To The Same Carbon Patents (Class 536/53)
  • Methods for use of genetically modified tumor-targeted bacteria with reduced virulence
    Publication number: 20030170276
    Abstract: The present invention is directed to mutant Salmonella sp. having a genetically modified msbB gene in which the mutant Salmonella is capable of targeting solid tumors. The present invention further relates to the therapeutic use of the mutant Salmonella for growth inhibition and/or reduction in volume of solid tumors.
    Type: Application
    Filed: April 18, 2002
    Publication date: September 11, 2003
    Applicant: Vion Pharmaceuticals, Inc.
    Inventors: David Bermudes, Kenneth Brooks Low
  • Drug complex
    Publication number: 20030171262
    Abstract: A drug complex characterized in that a residue of a drug compound such as antineoplastic agents and a carboxy(C1-4)alkyldextran polyalcohol obtained by treating a dextran under conditions that enable substantially complete polyalcoholization are bound to each other by means of a spacer comprising an amino acid or a spacer comprising peptide-bonded 2 to 8 amino acids. Said complex is characterized in that it has excellent selectivity to tumorous sites so as to exhibit high antineoplastic activity and also achieves reduced appearance of toxicity.
    Type: Application
    Filed: May 28, 2002
    Publication date: September 11, 2003
    Applicant: Daiichi Pharmaceutical Co., Ltd.
    Inventors: Kazuhiro Inoue, Hiroshi Susaki, Masahiro Ikeda, Hiroshi Kuga, Eiji Kumazawa, Akiko Togo
  • Synthesis of complex carbohydrates
    Publication number: 20030170828
    Abstract: A tailor-assembly approach is employed for synthesis of complex carbohydrates wherein a polysaccharide is degraded and the shorter product obtained from the degradation is subjected to enzymatic modification to add a sugar moiety. The products may be useful in the preparation of a cancer vaccine. In one example, oligosaccharides of the type Ia group B Streptococcus (GBSIa) capsular polysaccharide and multivalent sialyl Lex antigens are specifically described. GBSIa polysaccharide was depolymerized by partial Smith degradation to fragments representing asialo core repeating units. Enzymatic sialylation of these oligomers furnished GBSIa repeating units (from monomer to pentamer). Fucosylation on GlcNAc residues of GBSIa oligomers afforded oligosaccharides that carry multiple sialyl Lex epitopes.
    Type: Application
    Filed: February 19, 2003
    Publication date: September 11, 2003
    Inventors: Wei Zou, Harold J Jennings
  • Sphingolpid ceramide N-deacylase, methods for producing sphingolipids and sphingolipid derivatives, and sphingolipid ceramide N-deacylase gene
    Publication number: 20030166250
    Abstract: The present invention relates to a novel sphingolipid ceramide N-deacylase (SCDase) having a wide substrate specificity; a method for enzymatically producing a lysosphingolipid or a sphingolipid derivative using the SCDase which is useful in the fields of medicine, carbohydrate engineering, cell engineering, and the like; the lysosphingolipid or sphingolipid derivative obtained by this production method; a gene which encodes a polypeptide having an SCDase activity useful in sphingolipid technology; a method for industrially producing a polypeptide having an SCDase deacylase activity and a recombinant polypeptide thereof using a transformant to which the gene is introduced; a probe or primer which hybridizes to the gene; and an antibody or a fragment thereof which specifically binds to the polypeptide.
    Type: Application
    Filed: May 20, 2002
    Publication date: September 4, 2003
    Applicant: TAKARA SHUZO CO., LTD.
    Inventors: Makoto Ito, Toyohisa Kurita, Katsuhiro Kita, Noriyuki Sueyoshi, Susumu Mitsutake, Masanori Fujita, Nozomu Okino, Hiroyuki Izu, Ikunoshin Kato
  • Dds compound and process for the preparation thereof
    Publication number: 20030166513
    Abstract: A DDS compound in which amino group at 1-position of (1S,9S)-1-amino-9-ethyl-5-fluoro-2,3-dihydro-9-hydroxy-4-methyl-1H,12H-benzo[de]pyrano[3′,4′:6,7]-indolizino[1,2-b]quinoline-10,13(9H,15H)-dione as a drug compound is bound to a carboxyl group of a carboxymethyldextran polyalcohol with a spacer containing one amino acid or two to eight amino acids linked by peptide bond(s); characterized in that an introduced amount of residue of the drug compound is in a range of from 3.2% to 8.4% by weight; a weight-average molecular weight of the carboxymethyldextran polyalcohol is in a range of from 240,000 to 480,000; and degree of carboxymethylation is in a range of from 0.14 to 0.47; and a method for preparing said DDS compound, which comprises the steps of, for example, adding an aqueous solution containing sodium periodate to an aqueous solution containing dextran at a temperature of 4° C.±2° C.
    Type: Application
    Filed: May 2, 2003
    Publication date: September 4, 2003
    Inventors: Akihiro Imura, Shigeru Noguchi, Tatsuya Yamaguchi, Tsutomu Yagi, Takefumi Kawabe
  • C-glucosyl ether lipids
    Patent number: 6613748
    Abstract: A C-glucosyl ether lipid of the following formula: wherein R1 is a C12-C20 alkyl or C12-C20 alkenyl; R2 is a C1-C3 alkyl or C3 cycloalkyl; and X nitrogen-containing group.
    Type: Grant
    Filed: December 11, 2001
    Date of Patent: September 2, 2003
    Assignees: The Research Foundation of the City University of New York, The University of Manitoba
    Inventors: Robert Bittman, Gilbert Arthur, Richard W. Frank
  • Polymers and their use
    Publication number: 20030162688
    Abstract: A substituted polysaccharide comprising &bgr;1-4 linkages having covalently bonded on the polysaccharide moiety thereof, at least one deposition enhancing group which undergoes a chemical change in water at a use temperature to increase the affinity of the substituted polysaccharide to a substrate, the substituted polysaccharide further comprising one or more independently selected silicone chains.
    Type: Application
    Filed: August 22, 2002
    Publication date: August 28, 2003
    Applicant: Unilever Home & Personal Care USA, Division of Conopco, Inc.
    Inventors: Paul Hugh Findlay, Christopher Clarkson Jones, Dax Kukulj
  • Chromogenic substrates of sialidase and methods of making and using the same
    Publication number: 20030162240
    Abstract: The subject invention discloses materials and methods for the design, synthesis, and biochemical evaluation of chromogenic substrate compounds for sialidases of bacterial, viral, protozoa, and vertebrate (including humans) origin. In particular, this invention provides a novel class of effective compounds as chromogenic substrates of these sialidases which yield chromogenic products after reactions catalyzed by sialidase take place. Also provided are methods of making these substrate compounds, methods of diagnosis and prognosis of sialidase related diseases using these substrate compounds.
    Type: Application
    Filed: January 21, 2003
    Publication date: August 28, 2003
    Inventors: Stephen C. Johnson, Ashraf Saeed, Ming Luo
  • Combination of aminosugars and cysteine or cysteine derivatives
    Publication number: 20030162732
    Abstract: The present invention relates to chemical complexes consisting of cysteine or derivatives of cysteine and an aminosugar as well as pharmaceutical compositions and dietary supplements comprising such complexes. The invention further relates to the use of such compositions or complexes for the preparation of a medicament or a dietary supplement in the suppression of hypersensitivity and inflammatory reactions such as rheumatic or dermatological disorders or to a method of treating such diseases by administering such compositions and complexes.
    Type: Application
    Filed: June 28, 2002
    Publication date: August 28, 2003
    Applicant: Astion Development A/S
    Inventor: Morten Sloth Weidner
  • Nucleic acids encoding polypeptides with beta-1,3-galactosyl transferase activity
    Publication number: 20030157655
    Abstract: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an &agr;2,3- and an &agr;2,8-activity. A &bgr;1,4-GalNAc transferase and a &bgr;1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
    Type: Application
    Filed: November 21, 2002
    Publication date: August 21, 2003
    Applicant: National Research Council of Canada
    Inventors: Michel Gilbert, Warren W. Wakarchuk
  • Polypeptides having beta-1,3-galactosyl transferase activity
    Publication number: 20030157657
    Abstract: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an &agr;2,3- and an &agr;2,8-activity. A &bgr;1,4-GalNAc transferase and a &bgr;1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
    Type: Application
    Filed: November 21, 2002
    Publication date: August 21, 2003
    Applicant: National Research Council of Canada
    Inventors: Michel Gilbert, Warren W. Wakarchuk
  • Nucleic acids encoding beta-1,4-GaINAc transferase
    Publication number: 20030157656
    Abstract: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an &agr;2,3- and an &agr;2,8-activity. A &bgr;1,4-GalNAc transferase and a &bgr;1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
    Type: Application
    Filed: November 21, 2002
    Publication date: August 21, 2003
    Applicant: National Research Council of Canada
    Inventors: Michel Gilbert, Warren W. Wakarchuk
  • Polypeptides having beta-1,4-GalNAc transferase activity
    Publication number: 20030157658
    Abstract: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an &agr;2,3- and an &agr;2,8-activity. A &bgr;1,4-GalNAc transferase and a &bgr;1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
    Type: Application
    Filed: November 21, 2002
    Publication date: August 21, 2003
    Applicant: National Research Council of Canada
    Inventors: Michel Gilbert, Warren W. Wakarchuk
  • Functionalized tannin and improved protein extractant
    Publication number: 20030158124
    Abstract: A tannin functionalized with an aliphatically unsaturated, hydroxy reactive compound selected from the group of a carboxylic acid, a carboxylic acid ester, an isocyanate and an epoxide, which functionalized tannin is particularly useful in crosslinked form as an extractant in beverage clarification.
    Type: Application
    Filed: February 20, 2002
    Publication date: August 21, 2003
    Applicant: ISP INVESTMENTS INC.
    Inventors: Jenn S. Shih, Mustafa Rehmanji, Michael A. Drzewinski
  • Remedies for joint diseases
    Patent number: 6608043
    Abstract: The present invention provides an agent for treatment of arthritic diseases such as rheumatoid arthritis that has for its active ingredient a complex of hyaluronic acid and zinc. This complex synergistically inhibits proliferation of synovial cells and suppresses matrix metalloproteinase MMP-9, which is produced by synovial cells, as compared with its constituents, hyaluronic acid and zinc, alone.
    Type: Grant
    Filed: September 7, 2001
    Date of Patent: August 19, 2003
    Assignees: Takata Seiyaku Co., Ltd., Richter Gedeon Vegyeszeti Gyar Rt.
    Inventors: Isao Serizawa, Keisei Maekawa, Janos Illes, Erzsebet Neszmeli
  • Novel glycoconjugates, glycoamino acids, intermediates thereto, and uses thereof
    Publication number: 20030153492
    Abstract: The present invention provides novel glycosides and glycoconjugates, glycoamino acids, and methods for the synthesis thereof. In another aspect, the present invention provides novel clustered glycopeptides and methods for the synthesis thereof. In still another aspect, the present invention provides methods for the treatment of cancer, preferably for the prevention of recurrence of cancer, and methods for inducing antibodies in a subject, comprising administering to a subject in need, an effective amount of any of the inventive glycopeptides as disclosed herein, either in conjugated form or unconjugated and in combination with a suitable immunogenic carrier.
    Type: Application
    Filed: July 31, 2002
    Publication date: August 14, 2003
    Inventors: Samuel J. Danishefsky, Don M. Coltart, Stacy J. Keding, Kaustav Biswas, Philip O. Livingston, Govindaswami Ragupathi, Jennifer R. Allen, Lawrence Williams
  • Tissues or organs for use in xenotransplantation
    Publication number: 20030153044
    Abstract: The present invention provides cells, tissues or organs for use in cell therapy or xenotransplantation in which at least one gene comprising an antigenic determinant recognized by a recipient organism has been disrupted. The present invention also includes methods of administering such cells and transplanting such tissues or organs in which genes encoding antigenic determinants recognized by the recipient organism have been disrupted.
    Type: Application
    Filed: November 21, 2002
    Publication date: August 14, 2003
    Inventors: Monika Liljedahl, Daniela Marcantonio, Simon Eric Aspland
  • Polysaccharide crosslinking, hydrogel preparation, resulting polysaccharide(s) and hydrogel(s), uses thereof
    Publication number: 20030148995
    Abstract: The invention relates to a process for the crosslinking of polysaccharides. The process of the invention is a process for the crosslinking of at least one polymer selected from polysaccharides and their derivatives, under the action of at least one polyfunctional crosslinking agent, characterized in that said polyfunctional crosslinking agent is reacted with said polymer, in the solid state, during hydration. The invention is applied to the manufacture of hydrogels and gels that can be used, in particular, in plastic or cosmetic surgery.
    Type: Application
    Filed: January 13, 2003
    Publication date: August 7, 2003
    Inventors: Estelle Piron, Raymonde Tholin
  • Process for producing desulfated polysaccharide, and desulfated heparin
    Publication number: 20030149253
    Abstract: This invention relates to a process for producing a desulfated polysaccharide, which comprises reacting a sulfated polysaccharide having a saccharide in which a primary hydroxyl group is sulfated, as a constituent sugar, with a silylating agent represented by the following formula (I) 1
    Type: Application
    Filed: December 6, 2002
    Publication date: August 7, 2003
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Saburo Hara, Keiichi Yoshida, Masayuki Ishihara
  • Sialidase and recombinant cell lines
    Publication number: 20030148493
    Abstract: A recombinant cell line has a constitutive sialidase whose functional expression is disrupted, for example by homologous recombination or using antisense RNA. Sialidase is purified from cell culture fluid of Chinese hamster ovary cells. Nucleic acid encoding sialidase is obtained using an oligonucleotide probe designed using amino acid sequence data on the sialidase.
    Type: Application
    Filed: December 19, 2002
    Publication date: August 7, 2003
    Applicant: Genentech, Inc.
    Inventors: Thomas G. Warner, Mary B. Sliwkowski
  • Polypeptides having sialyltransferase activity
    Publication number: 20030148459
    Abstract: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an &agr;2,3- and an &agr;2,8-activity. A &bgr;1,4-GalNAc transferase and a &bgr;1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
    Type: Application
    Filed: November 21, 2002
    Publication date: August 7, 2003
    Applicant: National Research Council of Canada
    Inventors: Michel Gilbert, Warren W. Wakarchuk
  • Fluorinated glucosamine analogs useful for modulating post-translational glycosylations on cells
    Publication number: 20030148997
    Abstract: The present invention provides compositions and methods for inhibiting cell migration, e.g., lymphocytes and inflammation. The invention also provides an improved process for preparing fluorinated N-acetylglucosamines.
    Type: Application
    Filed: November 26, 2002
    Publication date: August 7, 2003
    Inventors: Robert Sackstein, Charles J. Dimitroff, Ralph J. Bernacki, Moheswar Sharma, Khushi L. Matta, Brajeswar Paul
  • Photocurable hyaluronic acid derivative and process for producing the same, and photocured crosslinked-hyaluronic acid derivative and medical material using the same
    Patent number: 6602859
    Abstract: A photocurable hyaluronic acid derivative of the present invention comprises hyaluronic acid and a photoreactive crosslinking group bonded thereto, wherein a 1.0 wt. % aqueous solution of the photocurable hyaluronic acid derivative obtained by dissolving the solid photocurable hyaluronic acid derivative in an aqueous solvent, is capable of passing through a porous filter having a pore size of 0.45 &mgr;m and a diameter of 25 mm at a rate of not less than 2 ml/minute at 24° C. under a pressure of 5.0 Kg/cm2; and a process for producing the photocurable hyaluronic acid derivative; and a photocured crosslinked-hyaluronic acid derivative; a medical material using the photoreactive hyaluronic acid derivative and a medical material using the photocured crosslinked-hyaluronic acid derivative.
    Type: Grant
    Filed: December 18, 2001
    Date of Patent: August 5, 2003
    Assignee: Seikagaku Corporation
    Inventors: Kenji Miyamoto, Yasuhiro Kurahashi, Hiroki Kano
  • Novel glycosidase inhibitors and their pharmacological uses, in particular for treating diabetes
    Publication number: 20030143713
    Abstract: The invention concerns the use of a polyamine derivative or a polyamine for inhibiting the active site of glycosidase enzymes intervening in the transformation of polysaccharides into sugars, in particular into glucose, in a living organism.
    Type: Application
    Filed: October 24, 2002
    Publication date: July 31, 2003
    Inventors: Nushin Banu Helene Aghajari, Xavier Guy Robert, Richard Michel Haser
  • Substituted liposaccharides useful in the treatment and prevention of endotoxemia
    Publication number: 20030144503
    Abstract: Novel substituted liposaccharides useful as in the prophylactic and affirmative treatment of endotoxemia including sepsis, septicemia and various forms of septic shock and methods of using these agents are provided. Also provided are methods of preparing these agents and intermediates useful therein.
    Type: Application
    Filed: May 13, 2002
    Publication date: July 31, 2003
    Inventors: William J. Christ, Daniel P. Rossignol
  • Anti-sepsis conjugate vaccine
    Publication number: 20030138448
    Abstract: The present invention provides an immunogenic conjugate comprising biologically deacylated gram-negative bacterial moieties linked to D. discoideum proteinase 1, as well as novel subunits thereof, and methods of making and using the conjugates in vaccines to treat sepsis and other infectious complications.
    Type: Application
    Filed: October 15, 2002
    Publication date: July 24, 2003
    Inventors: Gary L. Gustafson, Dan C. DeBorde
  • Prophylactic and therapeutic treatment of infectious and other diseases with mono- and disaccharide-based compounds
    Publication number: 20030139356
    Abstract: Methods and compositions for treating or ameliorating diseases and other conditions, such as infectious diseases, autoimmune diseases and allergies are provided. The methods employ mono- and disaccharide-based compounds for selectively stimulating immune responses in animals and plants.
    Type: Application
    Filed: November 20, 2001
    Publication date: July 24, 2003
    Inventors: David H. Persing, Richard T. Crane, Gary T. Elliot, J. Terry Ulrich, Michael J. Lacy, David A. Johnson, Jory R. Baldridge, Rong Wang
  • Methods for production of hyaluronic acid and isolated strains of supercapsulated streptococci
    Publication number: 20030134393
    Abstract: Isolated strains of supercapsulated streptococci bend at a density of no greater than 1.03 g/cm3 in a Percoll gradient and are capable of producing hyaluronic acid with molecular weight exceeding 6 million Da. Methods of producing high molecular weight hyaluronic acid employ a supercapsulated strain of streptococcus which bands at a density of no greater than 1.03 g/cm3 in a Percoll gradient. Methods of selecting streptococcus strains capable of producing hyaluronic acid with a molecular weight exceeding 6 million Da comprise, inter alia, cultivating supercapsulated strains of streptococci which band at a density of no greater than 1.03 g/cm3 in a Percoll gradient.
    Type: Application
    Filed: January 29, 2003
    Publication date: July 17, 2003
    Inventor: Sten Stahl
  • Bacterial transglycosylases: assays for monitoring the activity using Lipid II substrate analogs and methods for discovering new antibiotics
    Publication number: 20030129683
    Abstract: This invention provides a direct method for monitoring bacterial transglycosylase activity using labeled substrates produced by chemo-enzymatic synthesis wherein the labels are selected to permit the detection of both polymeric and non-polymeric products simultaneously, either directly or following the separation of product from starting material. The invention promotes the discovery of new antibiotics with activity against bacterial transglycosylases by a) laying the groundwork for structural analysis of purified, active transglycosylase (which permits structure-based design); and b) providing an assay that can be used to screen for inhibitors.
    Type: Application
    Filed: October 2, 2002
    Publication date: July 10, 2003
    Inventor: Suzanne Walker Kahne
  • Practical in vitro sialylation of recombinant glycoproteins
    Publication number: 20030124645
    Abstract: This invention provides methods for practical in vitro sialylation of glycoproteins, including recombinantly produced glycoproteins. The methods are useful for large-scale modification of sialylation patterns.
    Type: Application
    Filed: August 13, 2002
    Publication date: July 3, 2003
    Applicant: Neose Technologies, Inc.
    Inventors: James C. Paulson, Robert J. Bayer, Eric Sjoberg
  • Novel proteins involved in the synthesis and assembly of O-antigen in pseudomonas aeruginosa
    Publication number: 20030124634
    Abstract: Novel nucleic acid molecules encoding proteins involved in the synthesis and assembly of O-antigen in P. aeruginosa; and novel proteins encoded by the nucleic acid molecules are described. Methods are disclosed for detecting P.aeruginosa in a sample by determining the presence of the proteins or a nucleic acid molecule encoding the proteins in the sample.
    Type: Application
    Filed: August 12, 2002
    Publication date: July 3, 2003
    Applicant: University of Guelph
    Inventors: Joseph S. Lam, Lori L. Burrows, Deborah Charter, Teresa de Kievit
  • Forming and modifying dielectrically-engineered microparticles
    Publication number: 20030119057
    Abstract: Engineered microparticles, libraries of microparticles, and methods relating thereto. The microparticles are distinguishable based on differences in dielectric response to an applied electric field. In different embodiments, the dielectric differences may be engineered through, but not limited to, dielectrically dispersive materials, surface charge, and/or fluorescence. Gangliosides may be incorporated with the microparticles to control aggregation. Vesicles including erythrocyte ghosts may be used as a basis for microparticles. The microparticles may utilize a biotin streptavidin system for surface functionalization.
    Type: Application
    Filed: December 20, 2001
    Publication date: June 26, 2003
    Applicant: Board of Regents
    Inventors: Peter R.C. Gascoyne, Jody Vykoukal, Daynene Vykoukal, Susan Sharma, Frederick F. Becker
  • Polysaccharide-polypeptide conjugate
    Publication number: 20030120033
    Abstract: Disclosed is a method of producing a polysaccharide-polypeptide conjugate by reacting a polysaccharide with a polypeptide which contains at least one free amino group, wherein a polysaccharide carrier comprising vicinal hydroxyl groups is oxidized under ring opening to create vicinal aldehyde groups and is reacted with one or more base-instable antigenic polypeptide(s) containing at least one free amino group, the polypeptide(s) being bound directly to the polysaccharide carrier via at least one azomethine bond.
    Type: Application
    Filed: September 17, 2002
    Publication date: June 26, 2003
    Inventors: Hans Loibner, Helmut Eckert
  • Hyaluronic acid microspheres for sustained gene transfer
    Publication number: 20030114406
    Abstract: A microsphere composition comprising materials which include substances which provide increased safety and bioavailability of nucleic acids when used in gene therapy applications. The microspheres of the present invention include hyaluronic acid which has been derivatized with a dihydrazide, preferably adipic dihydrazide, which is crosslinked to a nucleic acid. These microspheres are useful in gene therapy applications for the treatment of a variety of medical conditions, such as myocardial ischemia. In the treatment of myocardial ischemia, the microspheres of the invention include the VEGF gene. When cardiac cells are transfected with VEGF, angiogenesis in cardiac tissue is a result. Angiogenesis in cardiac tissue is likely to provide a therapeutic effect in the treatment of myocardial ischemia in that blocked or damaged blood vessels may be bypassed by newly grown blood vessels.
    Type: Application
    Filed: October 21, 2002
    Publication date: June 19, 2003
    Applicant: Collaborative Laboratories, Inc.
    Inventor: Weiliam Chen
  • Antithrombotic compound
    Publication number: 20030114361
    Abstract: The present invention relates to compounds of the formula (I), wherein R is independently SO3− or CH3; the spacer is a flexible spacer of a length of 13-25 atoms the charge of the pentasaccharide residue is compensated by positively charged counterions; and the total number of sulfate groups in the pentasaccharide residue is 4, 5 or 6; or a pharmaceutically acceptable salt, a prodrug or a solvate thereof. The compounds of the invention have antithrombotic activity and can be used in treating or preventing thrombin-related diseases.
    Type: Application
    Filed: August 29, 2002
    Publication date: June 19, 2003
    Inventors: Constant Adriaan Anton Van Boeckel, Cornelis Maria Tromp, Tamara Theodora Maria Geersten
  • DNA encoding hyaluronan synthase from pasturella multocida and methods of use
    Publication number: 20030113845
    Abstract: The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Pasturella multocida hyaluronate synthase (PmHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known as hyaluronic acid or hyaluronan. The present invention also relates to the use of the PmHAS in constructing “knock-out” mutant strains of P. multocida for use in vaccinations. The present invention further relates to the use of the PmHAS in diagnostic tests in the field determinations of livestock P. multocida infection.
    Type: Application
    Filed: August 12, 2002
    Publication date: June 19, 2003
    Inventor: Paul DeAngelis
  • Glycoconjugate vaccines for use in immune-compromised populations
    Publication number: 20030113350
    Abstract: Staphylococcal and Enterrococcal glycoconjugate vaccines are disclosed for use in preventing or treating bacterial infection in an immune-compromised individual. Such vaccines contain an immunocarrier and a conjugate of a polysaccharide or glycopeptide surface antigen from a clinically-significant bacterial strain. The vaccines can be used for active protection in immune-compromised individuals who are to be subjected to conditions that place them at immediate risk of developing a bacterial infection, as would be case in the context of a catheterization or a surgical procedure.
    Type: Application
    Filed: September 19, 2001
    Publication date: June 19, 2003
    Inventors: Ali I. Fattom, Robert B. Naso
  • Biomaterials comprising N-sulphated hyaluronic acid compounds or derivatives thereof
    Patent number: 6579978
    Abstract: The present invention is directed to novel sulphated compounds of hyaluronic acid and derivatives thereof, optionally salified, wherein the glucosamines are partially N-sulphated or partially N-sulphated and partially or totally O-sulphated in position 6. The compounds of the invention have anticoagulant and antithrombotic activities and are useful in the preparation of pharmaceutical compositions and biomaterials and in the production of coatings for biomedical objects.
    Type: Grant
    Filed: December 6, 1999
    Date of Patent: June 17, 2003
    Assignee: Fidia Farmaceuti S.p.A.
    Inventors: David Renier, Lanfranco Callegaro
  • Use of heparinoid derivatives for the treatment and diagnosis of disorders which can be treated with heparinoids
    Publication number: 20030109491
    Abstract: Heparinoid derivatives comprising a chelating agent which is covalently bonded to the heparinoid, and a paramagnetic metal cation from the series of transition metals Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Mo, Ru or of the lanthanides, are suitable for producing medicaments both for therapy and for diagnostic purposes, for localizing the dose employed, and for monitoring the result of treatment of disorders such as thrombosis and osteoarthrosis.
    Type: Application
    Filed: August 19, 2002
    Publication date: June 12, 2003
    Inventors: Wolfgang Ulmer, Hans-Paul Juretschke, Christopher Kern
  • Lipopolysaccharides (lps) extracted from escherichia coli
    Publication number: 20030108573
    Abstract: Lipopolysaccharides and processes for producting the lipopolysaccharides are provided. The lipopolysaccharide has a lipid A portion, a core oligosaccharide portion, and an O-specific chain having a single repeating unit 06. A lipopolysaccharide may be produced by washing and drying and E. coli bacterial mass, subjecting the washed and dried bacterial mass to a phenol/water extraction, and treating the extract with RNases, DNases, and proteinase K.
    Type: Application
    Filed: December 23, 2002
    Publication date: June 12, 2003
    Inventors: Hans Proppert, Jurgen Malinka, Jurgen Schulze, Ulrich Sonnenborn, Ulrich Zahringer, Artur Ulmer, Ernst Theodor Rietschel
  • Process for producing N-acetylneuraminic acid
    Publication number: 20030109007
    Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.
    Type: Application
    Filed: January 9, 2003
    Publication date: June 12, 2003
    Applicant: KYOWA HAKKO KOGYO CO., LTD.
    Inventors: Satoshi Koizumi, Kazuhiko Tabata, Tetsuo Endo, Akio Ozaki
  • Genetically modified tumor-targeted bacteria with reduced virulence
    Publication number: 20030109026
    Abstract: The present invention is directed to mutant Salmonella sp. having a genetically modified msbB gene in which the mutant Salmonella is capable of targeting solid tumors. The invention is also directed to Salmonella sp. containing a genetically modified msbB gene as well as an genetic modification in a biosynthetic pathway gene such as the purI gene. The present invention further relates to the therapeutic use of the mutant Salmonella for growth inhibition and/or reduction in volume of solid tumors.
    Type: Application
    Filed: June 27, 2002
    Publication date: June 12, 2003
    Applicant: Vion Pharmaceuticals, Inc.
    Inventors: David Bermudes, Kenneth Brooks Low
  • Thioester polymer derivatives and method of modifying the N-terminus of a polypeptide therewith
    Publication number: 20030105224
    Abstract: The invention provides reagents and methods for conjugating polymers specifically to the &agr;-amine of polypeptides in high yield. The invention provides monofunctional, bifunctional, and multifunctional PEGs and related polymers having a thioester moiety capable of specifically conjugating to the &agr;-amine of a polypeptide having a cysteine or histidine at the N-terminus. The invention provides active thioester derivatives of PEG that have suitable reactivity with an N-terminal cysteine or histidine residue of a polypeptide to produce an amide bond between the PEG and polypeptide. Use of these active esters to prepare PEG-proteins and PEG-peptides is described.
    Type: Application
    Filed: October 9, 2001
    Publication date: June 5, 2003
    Applicant: Shearwater Corporation
    Inventors: Michael J. Roberts, Zhihao Fang
  • Phophylactic and therapeutic treatment of infectious and other diseases with mono-and disaccharide-based compounds
    Publication number: 20030105032
    Abstract: Methods and compositions for treating or ameliorating diseases and other conditions, such as infectious diseases, autoimmune diseases and allergies are provided. The methods employ mono- and disaccharide-based compounds for selectively stimulating immune responses in animals and plants.
    Type: Application
    Filed: April 22, 2002
    Publication date: June 5, 2003
    Inventors: David H. Persing, Richard Thomas Crane, Gary T. Elliott, J. Terry Ulrich, Michael J. Lacy, David A. Johnson, Jory R. Baldridge, Rong Wang
  • Pneumococcus polysaccharide conjugates for use as vaccine against tetanus an diphtheria
    Publication number: 20030099672
    Abstract: The invention relates to the use of a composition comprising n Streptococcus pneumoniae polysaccharides conjugated to the tetanus toxoid and p Streptococcus pneumoniae polysaccharides conjugated to the diphtheria toxoid, for manufacturing a vaccine which protects against Clostridium tetani and/or Corynebacterium diphtheriae infections in which:
    Type: Application
    Filed: September 17, 2002
    Publication date: May 29, 2003
    Inventor: Dominique Schultz
  • Sodium hyaluronate microspheres
    Publication number: 20030096734
    Abstract: The present invention relates to microspheres comprising hyaluronan derivatized with a bifunctional crosslinker to form microspheres. Methods of making such microspheres, comprising mixing hyaluronic acid and a dihydrazide with a crosslinker in an aqueous solution, adding a solvent and an emulsifying agent to form an emulsion, and lowering the pH of the emulsion to allow intramolecular and intermolecular crosslinking to occur, are also disclosed. The invention also provides for pharmaceutical or cosmetic formulations based on the microspheres described herein, further containing one or more active or cosmetic agents, and methods of using such formulations.
    Type: Application
    Filed: December 5, 2002
    Publication date: May 22, 2003
    Applicant: Clear Solutions Biotech, Inc.
    Inventors: Philip Dehazya, Cheng Lu
  • Methods and compositions for production of lipo-chito oligosaccharides by rhizobacteria
    Publication number: 20030096375
    Abstract: Lipo-chito oligosaccharides (LCOs) are produced by culturing rhizobacteria cells in or on a culture medium comprising at least one of: jasmonic acid or a derivative thereof; linoleic acid or a derivative thereof; or linolenic acid or a derivative thereof. Preferably, the rhizobacteria cells are Bradyrhizobium japonicum cells having the identifying characteristics of B. japonicum strain USDA 3. Preferably, the derivative of jasmonic acid is an ester thereof, preferably methyl jasmonate. Also provided are methods for improving LCO production at low temperatures, particularly temperatures below 25° C.
    Type: Application
    Filed: May 16, 2002
    Publication date: May 22, 2003
    Inventors: Donald L. Smith, Fazli Mabood, Hao Zhang
  • Hydrophobe biomolecular structure
    Publication number: 20030096266
    Abstract: The present invention relates to a new hydrophobe biomolecular structure, which is compacted due to the passing of the structure over its point of collapse, a method for the preparation of the structure and use of the new structure for the manufacture of a medicament.
    Type: Application
    Filed: July 2, 2002
    Publication date: May 22, 2003
    Inventor: Gustaf Jederstrom
  • Aminoalkyl glucosaminide phosphate compounds and their use as adjuvants and immunoeffectors
    Publication number: 20030092643
    Abstract: Aminoalkyl glucosaminide phosphate (AGP) compounds that are adjuvants and immunoeffectors are described and claimed. The compounds have a 2-deoxy-2-amino glucose in glycosidic linkage with an aminoalkyl (aglycon) group. Compounds are phosphorylated at the 4 or 6 carbon on the glucosaminide ring and comprise three 3- alkanoyloxyalkanoyl residues. The compounds augment antibody production in immunized animals as well as stimulate cytokine production and activate macrophages. Compositions and methods for using the compounds as adjuvants and immunoeffectors are also disclosed.
    Type: Application
    Filed: January 8, 2002
    Publication date: May 15, 2003
    Applicant: Corixa Corporation
    Inventors: David A. Johnson, C. Gregory Sowell
  • Pharmaceutical composition for inhibiting the metastasis or preventing the recurrence of malignant tumor
    Publication number: 20030092608
    Abstract: A pharmaceutical composition for inhibiting the metastasis or preventing the recurrence of a malignant tumor, which comprises as the active ingredient a polysaccharide derivative comprising a polysaccharide having a carboxyl group bound to an active substance having an anti-tumor activity via an amino acid or a peptide consisting of 2 to 8 amino acids which are the same or different, or a salt thereof.
    Type: Application
    Filed: August 21, 2002
    Publication date: May 15, 2003
    Inventors: Takayuki Kawaguchi, Satoshi Okuno, Toshiro Yano