Bovine Patents (Class 800/15)
  • Patent number: 11274316
    Abstract: A method of expanding TCRalpha deficient T-cells by expressing pTalpha or functional variants thereof into said cells, thereby restoring a functional CD3 complex. This method is particularly useful to enhance the efficiency of immunotherapy using primary T-cells from donors. This method involves the use of pTalpha or functional variants thereof and polynucleotides encoding such polypeptides to expand TCRalpha deficient T-cells. Such engineered cells can be obtained by using specific rare-cutting endonuclease, preferably TALE-nucleases. The use of Chimeric Antigen Receptor (CAR), especially multi-chain CAR, in such engineered cells to target malignant or infected cells. The invention opens the way to standard and affordable adoptive immunotherapy strategies for treating cancer and viral infections.
    Type: Grant
    Filed: March 11, 2021
    Date of Patent: March 15, 2022
    Assignee: CELLECTIS
    Inventors: Roman Galetto, Agnes Gouble, Stephanie Grosse, Cecile Mannioui, Laurent Poirot, Andrew Scharenberg, Julianne Smith
  • Patent number: 11242366
    Abstract: According to aspects of the present invention, a peptide with any one sequence of SEQ ID NOS:1 to 3 exhibits high selective binding affinity to a target and the microcapsule has superior physicochemical stability. Therefore, the cosmetic composition containing the microcapsule linked to the peptide manifests high delivery efficiency of an active ingredient included in the capsule to target cells, thereby exhibiting superior skin-condition improvement effects.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: February 8, 2022
    Assignee: KOLMAR KOREA CO., LTD.
    Inventors: Sang Keun Han, Hyun Sook Lee, Eun Ah Kim, Seung Min Hyun, Hyeong Choi, So Yoon Baek, Jae Hwa Hong, Chae Mi Lim, Da Jeong Bak, Hye Jin Jo, Hak Sung Lee, Ji Hun Park, Eun Young Lee
  • Patent number: 10767195
    Abstract: The present invention relates in part to nucleic acids encoding proteins, therapeutics comprising nucleic acids encoding proteins, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods and products for altering the DNA sequence of a cell are described, as are methods and products for inducing cells to express proteins using synthetic RNA molecules. Therapeutics comprising nucleic acids encoding gene-editing proteins are also described.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: September 8, 2020
    Assignee: Factor Bioscience Inc.
    Inventors: Matthew Angel, Christopher Rohde
  • Patent number: 9745561
    Abstract: The genetic basis for severe combined immunodeficiency disease (SCID) in pigs is described. In addition, tests for detecting pigs that are carriers for SCID or pigs with SCID are also described. Further, methods for producing pigs or herds of pigs with SCID are also described. Further, methods and compositions for treating, ameilioraing, inhibiting or correcting SCID are provided.
    Type: Grant
    Filed: January 8, 2015
    Date of Patent: August 29, 2017
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Jack C. M. Dekkers, Christopher K. Tuggle, Emily H. Waide, Jason W. Ross, N. Matthew Ellinwood, Martine Schroyen
  • Patent number: 9085767
    Abstract: The present invention relates to a novel class of gene trap vector (enhanced gene trap vectors, eGTV) for efficiently identifying silent or weakly expressed target genes in mammalian genomes, methods of their production and methods for identifying and mutating target genes by using the enhanced gene trap vectors. The gene trap vectors of the present invention can also be used for inducing the expression of silent genes and enhancing the expression of weakly expressed genes. The use of the enhanced gene trap vectors for creating transgenic organisms to identify gene function and to validate pharmaceutical compounds prior to clinical applications is a further aspect of the present invention.
    Type: Grant
    Filed: November 28, 2005
    Date of Patent: July 21, 2015
    Assignees: FRANKGEN BIOTECHNOLOGIE AG, GSF FORSCHUNGSZENTRUM FUR UMWELT UND GESUNDHEIT GMBH, MPG MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V.
    Inventors: Harald Von Melchner, Frank Schnütgen, Wolfgang Wurst, Patricia Ruiz
  • Publication number: 20150150153
    Abstract: The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca2+ in the oocyte prior to reduction of the intracellular Zn2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods.
    Type: Application
    Filed: November 26, 2014
    Publication date: May 28, 2015
    Applicant: The Curators of The University of Missouri
    Inventors: Kiho Lee, Randall S. Prather
  • Patent number: 9006510
    Abstract: A method for producing a heart valve xenograft from bovine tissue expressing reduced or undetectable levels of I1-3 galactosyl transferase are provided.
    Type: Grant
    Filed: June 12, 2007
    Date of Patent: April 14, 2015
    Assignee: Mayo Foundation for Medical Education and Research
    Inventors: Guerard W. Byrne, Christopher G. A. McGregor
  • Publication number: 20150067899
    Abstract: The present invention relates to the field of biotechnology or genetic engineering. More specifically, the present invention relates to a multiple inducible gene regulation system that functions within cells to simultaneously control the quantitative expression of multiple genes.
    Type: Application
    Filed: September 5, 2014
    Publication date: March 5, 2015
    Inventors: Tarlochan Singh DHADIALLA, Dean Ervin Cress, Glenn Richard Carlson, Robert Eugene Hormann, Subba Reddy Palli, Arthur John Kudla, Ronald Phillip Herzig, Mohan Philip
  • Publication number: 20150013025
    Abstract: Disclosed are materials and methods for creating customizable traits in animals. In the demonstration of the principle of the subject invention, a keratin-14 specific promoter is used with red fluorescent protein in the loxp cassette, dominant black (?G23) beta defensin 103 in the pigment cassette, and an SV40 (with intron) polyadenylation sequence. When Cre recombinase (or HTNCre) is applied to the animal's skin in a carrier base (e.g., lipid bilayers), fur is permanently genetically modified to turn black in the shape in which the HTNCre was applied.
    Type: Application
    Filed: May 7, 2014
    Publication date: January 8, 2015
    Inventor: JAMES WEST
  • Publication number: 20150007359
    Abstract: The present invention is directed generally to eukaryotic cells comprising single-celled organisms that are introduced into the eukaryotic cell through human intervention and which transfer to daughter cells of the eukaryotic cell through at least five cell divisions, and methods of introducing such single-celled organisms into eukaryotic cells. The invention also provides methods of using such eukaryotic cells. The invention further provides single-celled organisms that introduce a phenotype to eukaryotic cells that is maintained in daughter cells. The invention additionally provides eukaryotic cells containing magnetotactic bacteria.
    Type: Application
    Filed: September 17, 2014
    Publication date: January 1, 2015
    Inventors: Caleb B. BELL, III, Alexey Bazarov
  • Patent number: 8912386
    Abstract: The present invention provides transgenic, large non-human animal models of diseases and conditions, as well as methods of making and using such animal models in the identification and characterization of therapies for the diseases and conditions.
    Type: Grant
    Filed: November 3, 2011
    Date of Patent: December 16, 2014
    Assignees: University of Iowa Research Foundation, The Curators of the University of Missouri
    Inventors: Michael J. Welsh, Christopher S. Rogers, Randall S. Prather, John Engelhardt, Ziying Yan
  • Publication number: 20140359796
    Abstract: A genetically modified livestock animal, and methods of making and using the same, the animal comprising a genetic modification to disrupt a target gene selectively involved in gametogenesis, wherein the disruption of the target gene prevents formation of functional gametes of the animal. Animals that create progeny with donor genetics, and methods of making and using the same. Cells, and methods of making and using the cells, with a genetic modification to disrupt a target gene selectively involved in gametogenesis.
    Type: Application
    Filed: April 28, 2014
    Publication date: December 4, 2014
    Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
  • Patent number: 8785718
    Abstract: Methods of using hypermethylated transposons to create genetically modified animals that express interfering RNAs are described.
    Type: Grant
    Filed: October 5, 2012
    Date of Patent: July 22, 2014
    Assignee: Recombinetics, Inc.
    Inventors: Scott C. Fahrenkrug, Daniel F. Carlson, Aron M. Geurts
  • Publication number: 20140201857
    Abstract: Compositions and methods for making livestock with a polled allele are presented, including migrating a polled allele into a bovine species without changing other genes or chromosomal portions.
    Type: Application
    Filed: January 14, 2014
    Publication date: July 17, 2014
    Applicant: Recombinetics, Inc.
    Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
  • Publication number: 20140182005
    Abstract: The present invention provides sperm cell targeting ligands, including DNA-binding proteins, that bind target molecules on the surface of, accessible from the surface of, or inside mammalian sperm cells and methods for producing the sperm cell targeting ligands. The sperm cell targeting ligands are used to separate sperm cells based upon sperm cell qualities, such as whether the cells contain X chromosomes or Y chromosomes. The invention also provides methods of sperm cell purification using targeted radiofrequency absorption enhancers and transgenic animals with sex-skewed ejaculate.
    Type: Application
    Filed: April 10, 2013
    Publication date: June 26, 2014
    Applicant: BIOCERN, INC.
    Inventor: Biocern, Inc.
  • Patent number: 8715155
    Abstract: An embryo that is transferred into the uterus of a recipient female is protected from toxic effects of prostaglandin F2? by exposing the embryo, or an oocyte from which the embryo is produced, to a prostaglandin antagonist.
    Type: Grant
    Filed: July 29, 2008
    Date of Patent: May 6, 2014
    Assignee: University of Tennessee Research Foundation
    Inventor: F. Neal Schrick
  • Publication number: 20140123330
    Abstract: A genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature. Methods of using, and processes of making, the animals are taught.
    Type: Application
    Filed: October 30, 2013
    Publication date: May 1, 2014
    Inventors: Daniel F. Carlson, Scott C. Fahrenkrug, Xavier Lauth
  • Patent number: 8703485
    Abstract: The invention relates to transgenic animals lacking endogenous Ig and capable of producing transgenic antibodies, as well as methods of making the same. The invention further relates to methods for producing transgenic antibodies in such animals, and transgenic antibodies so produced.
    Type: Grant
    Filed: May 30, 2008
    Date of Patent: April 22, 2014
    Assignee: OMT, Inc.
    Inventor: Roland Buelow
  • Publication number: 20140068796
    Abstract: A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.
    Type: Application
    Filed: October 18, 2013
    Publication date: March 6, 2014
    Applicant: CRYSTAL BIOSCIENCE, INC.
    Inventors: William Don Harriman, Robert Etches, Phil Leighton
  • Publication number: 20140053287
    Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.
    Type: Application
    Filed: August 14, 2013
    Publication date: February 20, 2014
    Applicant: VERENIUM CORPORATION
    Inventors: David P. WEINER, Lishan ZHAO, Peter LUGINBUHL, Ellen BURKE, Toby H. RICHARDSON, Paula M. HICKS
  • Patent number: 8598409
    Abstract: This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to a novel ecdysone receptor/chimeric retinoid X receptor-based inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy, large-scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.
    Type: Grant
    Filed: August 24, 2007
    Date of Patent: December 3, 2013
    Assignee: Intrexon Corporation
    Inventors: Marianna Zinovievna Kapitskaya, Subba Reddy Palli
  • Publication number: 20130298268
    Abstract: Disclosed are materials and methods for creating customizable traits in animals. In the demonstration of the principle of the subject invention, a keratin-14 specific promoter is used with, red fluorescent protein in the loxp cassette, dominant black (?G23) beta defensin 103 in the pigment cassette, and an SV40 (with intron) polyadenylation sequence. When Cre recombinase (or HTNCre) is applied to the animal's skin in a carrier base (e.g., lipid bilayers), fur is permanently genetically modified to turn black in the shape in which it was applied.
    Type: Application
    Filed: July 16, 2013
    Publication date: November 7, 2013
    Inventor: JAMES WEST
  • Patent number: 8569574
    Abstract: Single nucleotide polymorphic sites at positions 3117, 12195, 13244, 13319, and 13516 of the bovine STAT5 gene are associated with improved fertilization rate and/or improved embryo survival rate. Also disclosed are nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods.
    Type: Grant
    Filed: November 7, 2008
    Date of Patent: October 29, 2013
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Hasan Khatib, Ricky L. Monson
  • Publication number: 20130219533
    Abstract: In some aspects, the invention provides compositions and methods for inhibiting viral infection. In some aspects, the invention provides compositions and methods useful for identifying antiviral compounds.
    Type: Application
    Filed: June 17, 2011
    Publication date: August 22, 2013
    Applicant: WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
    Inventors: Thijn R. Brummelkamp, Jan E. Carette
  • Publication number: 20130212723
    Abstract: Disclosed are materials and methods for creating customizable traits in animals. In the demonstration of the principle of the subject invention, a keratin-14 specific promoter is used with red fluorescent protein in the loxp cassette, dominant black (?G23) beta defensin 103 in the pigment cassette, and an SV40 (with intron) polyadenylation sequence. When Cre recombinase (or HTNCre) is applied to the animal's skin in a carrier base (e.g., lipid bilayers), fur is permanently genetically modified to turn black in the shape in which the HTNCre was applied.
    Type: Application
    Filed: March 15, 2013
    Publication date: August 15, 2013
    Applicant: MICE WITH HORNS, LLC
    Inventor: Mice With Horns, LLC
  • Publication number: 20130212722
    Abstract: Disclosed are materials and methods for creating customizable traits in animals. In the demonstration of the principle of the subject invention, a keratin-14 specific promoter is used with, red fluorescent protein in the loxp cassette, dominant black (?G23) beta defensin 103 in the pigment cassette, and an SV40 (with intron) polyadenylation sequence. When Cre recombinase (or HTNCre) is applied to the animal's skin in a carrier base (e.g., lipid bilayers), fur is permanently genetically modified to turn black in the shape in which it was applied.
    Type: Application
    Filed: February 15, 2013
    Publication date: August 15, 2013
    Applicant: MICE WITH HORNS, LLC
    Inventor: Mice With Horns, LLC
  • Publication number: 20130131317
    Abstract: The invention features a process of expressing secreted recombinant human alpha-fetoprotein (rHuAFP) in the milk or urine of transgenic mammals.
    Type: Application
    Filed: January 25, 2013
    Publication date: May 23, 2013
    Applicant: Merrimack Pharmaceuticals, Inc.
    Inventor: Merrimack Pharmaceuticals, Inc.
  • Publication number: 20130039991
    Abstract: The application is in the field of transgenic (non-human) organisms, sialic acid chemistry, metabolism and antigenicity. More particularly, the invention is related to a method to produce Neu5Gc-free animals and products therefrom comprising disrupting the CMAH gene and thereby reducing or eliminating Neu5Gc from biological material of non-humans.
    Type: Application
    Filed: June 20, 2012
    Publication date: February 14, 2013
    Inventors: Ajit Varki, Anna Maria Hedlund, Dzung Nguyen
  • Publication number: 20130031648
    Abstract: The present relates to use of follistatin-like related gene (FLRG) to increase muscle mass in a subject. As such, methods of ameliorating the severity of a pathologic condition characterized, at least in part, by a decreased amount, development or metabolic activity of muscle are provided. In addition transgenic non-human mammals expressing FLRG and having increased muscle mass as compared to a corresponding mammal having a myostatin-null mutation or a decreased level of myostatin are provided.
    Type: Application
    Filed: August 6, 2012
    Publication date: January 31, 2013
    Inventor: Se-Jin Lee
  • Publication number: 20130024959
    Abstract: The present invention relates to a method of producing a cell comprising a conditionally active transgene in its genome, the method comprising (a) introducing into the cell a targeting vector, wherein the targeting vector comprises (i) a 5? recombinase recognition site specifically recognised by a first recombinase, wherein the first recombinase is endogenously present in the cell or wherein the first recombinase or a nucleic acid molecule encoding said first recombinase in expressible form is introduced into the cell; followed by (ii) a 5? recombinase recognition site specifically recognised by a second recombinase, wherein the second recombinase is not endogenously present or is not active in the cell; followed by (iii) a selection cassette comprising a positively selectable marker gene; followed by (iv) a 3? recombinase recognition site specifically recognised by a third recombinase, wherein the third recombinase is not endogenously present or is not active in the cell; followed by (v) the transgene; follo
    Type: Application
    Filed: November 24, 2010
    Publication date: January 24, 2013
    Applicants: UNIVERSITY OF FRANKFURT-MEDICAL SCHOOL, HELMHOLTZ ZENTRUM MÜNCHEN-DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH)
    Inventors: Laura Schebelle, Frank Schnütgen, Thomas Floss
  • Patent number: 8329980
    Abstract: The invention relates to the production of proteins and other substances of interest in saliva of transgenic animals, particularly in mammals that produce large quantities of saliva, particularly monogastric ruminants, and ovine, caprine and bovine mammals. Preferred embodiments of the invention relate in particular to the production of foreign and modified proteins in the transgenic saliva of these animals, including particularly human fibrinogen, human prothrombin and human thrombin, among others. The invention relates as well to methods, devices, genetic constructs and to transgenic constructs for making the proteins and other substances of interest, to novel saliva and saliva-derived compositions, novel products produced from the saliva, and to uses of the saliva, saliva-derived compositions and novel products.
    Type: Grant
    Filed: July 12, 2010
    Date of Patent: December 11, 2012
    Inventor: Jeffrey P. Erickson
  • Patent number: 8319008
    Abstract: The invention provides methods for managing livestock for breeding or production based on one or more measurements of mitochondrial function. Measurement of mitochondrial function may also be correlated with a calculated or known feed efficiency of livestock animals to yield a predicted feed efficiency for the animal. The invention overcomes deficiencies associated with phenotypic assays for predicted breeding and production value.
    Type: Grant
    Filed: January 14, 2011
    Date of Patent: November 27, 2012
    Assignee: The Curators of The University of Missouri
    Inventors: Monty S. Kerley, William Kolath, Joseph Golden
  • Publication number: 20120259093
    Abstract: The invention features a process of expressing secreted recombinant human alpha-fetoprotein (rHuAFP) in the milk or urine of transgenic mammals.
    Type: Application
    Filed: April 13, 2012
    Publication date: October 11, 2012
    Applicant: Merrimack Pharmaceuticals, Inc.
    Inventors: Stace LINDSAY, Robert Mulroy, Daniel Semeniuk
  • Publication number: 20120233715
    Abstract: A human artificial chromosome vector comprising a human antibody heavy chain gene, a human antibody light chain gene, and a human antibody surrogate light chain gene.
    Type: Application
    Filed: November 17, 2010
    Publication date: September 13, 2012
    Applicant: KYOWA HAKKO KIRIN CO., LTD
    Inventors: Yoshimi Kuroiwa, Hiroaki Matsushita, Akiko Sano
  • Publication number: 20120222140
    Abstract: The present invention provides a human artificial chromosome vector comprising a gene encoding the human antibody heavy chain, a gene encoding the human antibody light chain, and a gene encoding IgM heavy chain constant region derived from a nonhuman animal; and being capable of producing a human antibody with a higher efficiency when the vector is introduced into an animal. By immunizing the animal produced using a human artificial chromosome vector of the present invention with a desired antigen, a large quantity of human polyclonal antibodies can be supplied.
    Type: Application
    Filed: November 17, 2010
    Publication date: August 30, 2012
    Applicant: KYOWA HAKKO KIRIN CO., LTD.
    Inventors: Yoshimi Kuroiwa, Hiroaki Matsushita, Akiko Sano
  • Publication number: 20120222143
    Abstract: Compositions and methods for use of TALENs to make genetically modified livestock are set forth. The methods may include reporters for selecting cells or embryos that have been modified by TALENs for use as progenitor cells to make founder animals.
    Type: Application
    Filed: February 24, 2012
    Publication date: August 30, 2012
    Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
  • Publication number: 20120185954
    Abstract: This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.
    Type: Application
    Filed: November 4, 2011
    Publication date: July 19, 2012
    Applicant: INTREXON Corporation
    Inventors: Subba Reddy PALLI, Mohan Basavaraju Kumar
  • Publication number: 20120167239
    Abstract: This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to a novel ecdysone receptor/invertebrate retinoid X receptor-based inducible gene expression system and methods of modulating gene expression in a host cell for applications such as gene therapy, large-scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.
    Type: Application
    Filed: February 20, 2002
    Publication date: June 28, 2012
    Applicant: TUTOGEN MEDICAL GMBH
    Inventors: Subba Reddy Palli, Marianna Z. Kapitskaya
  • Publication number: 20120159653
    Abstract: The present invention provides genetically modified animals and cells comprising edited chromosomal sequences encoding proteins associated with MD. In particular, the animals or cells are generated using a zinc finger nuclease-mediated editing process. Also provided are methods of using the genetically modified animals or cells disclosed herein to study MD development and methods of assessing the effects of agents in genetically modified animals and cells comprising edited chromosomal sequences encoding proteins associated with MD.
    Type: Application
    Filed: July 23, 2010
    Publication date: June 21, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Edward Weinstein, Xiaoxia Cui, Phil Simmons
  • Publication number: 20120159654
    Abstract: The present invention provides genetically modified animals and cells comprising edited chromosomal sequences involved in ADME and toxicology. In particular, the animals or cells are generated using a zinc finger nuclease-mediated editing process. The invention also provides zinc finger nucleases that target chromosomal sequence involved in ADME and toxicology and the nucleic acids encoding said zinc finger nucleases. Also provided are methods of assessing the effects of agents in genetically modified animals and cells comprising edited chromosomal sequences involved in ADME and toxicology.
    Type: Application
    Filed: July 23, 2010
    Publication date: June 21, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Edward Weinstein, Xiaoxia Cui, Phil Simmons
  • Publication number: 20120128682
    Abstract: An isolated human Apolipoprotein L-I corresponding to a wild type human Apolipoprotein sequence is modified by a deletion at its C-terminal end.
    Type: Application
    Filed: August 18, 2010
    Publication date: May 24, 2012
    Applicant: Universite Libre de Bruxelles
    Inventors: Etienne Pays, Laurence Lecordier, Benolt Vanhollebeke
  • Publication number: 20120066781
    Abstract: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate.
    Type: Application
    Filed: May 20, 2010
    Publication date: March 15, 2012
    Inventors: David P. Weiner, Arne I. Solbak, JR., Ryan McCann
  • Publication number: 20120023599
    Abstract: The present invention provides genetically modified animals and cells comprising edited chromosomal sequences encoding cytochrome P450 (CYP) proteins. In particular, the animals or cells are generated using a zinc finger nuclease-mediated editing process. The invention also provides zinc finger nucleases that target chromosomal sequence encoding CYP proteins, as well as methods of using the genetically modified animals or cells disclosed herein to screen agents for toxicity and other effects.
    Type: Application
    Filed: July 23, 2010
    Publication date: January 26, 2012
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Edward Weinstein, Xiaoxia Cui, Victoria Brown-Kennerly
  • Publication number: 20110296544
    Abstract: The present invention provides a method for diagnosing and detecting diseases associated with colon. The present invention provides one or more proteins or fragments thereof, peptides or nucleic acid molecules differentially expressed in colon diseases (CCAT) and antibodies binds to CCAT. The present invention provides that CCAT is used as targets for screening agents that modulates the CCAT activities. Further the present invention provides methods for treating diseases associated with colon.
    Type: Application
    Filed: May 16, 2011
    Publication date: December 1, 2011
    Applicant: CELERA CORPORATION
    Inventors: Bruno DOMON, Alqun Li, Tao He, Ian McCaffery
  • Publication number: 20110296545
    Abstract: Particular aspects show that the signal peptide remains intact on the mature CD18 molecule on ruminant leukocytes rendering these cells susceptible to cytolysis by Lkt. Comparative amino acid sequence analysis of the signal peptide of CD18 of eight ruminants and five non-ruminants revealed that the ruminant CD18 signal peptides contain ‘cleavage-inhibiting’ glutamine (Q), compared to ‘cleavage-conducive’ glycine in non-ruminants, at position ?5 relative to the cleavage site. Mutagenesis of Q at position ?5 of the bovine CD18 signal peptide to G resulted in the abrogation of Lkt-mediated cytolysis of transfectants expressing bovine CD18 carrying the Q(?5)G mutation. Provided is novel technology to clone cattle and other ruminants expressing CD18 without the signal peptide on their leukocytes, providing ruminants that are less susceptible to M. haemolytica. Methods for treating conditions and/or diseases associated with M. haemolytica (e.g.
    Type: Application
    Filed: February 2, 2010
    Publication date: December 1, 2011
    Applicant: Washington State University
    Inventors: Subramaniam Srikumaran, Sudarvili Shanthalingam
  • Publication number: 20110283375
    Abstract: The invention provides, among other things, activatable prothrombin, compositions comprising prothrombin, transgenic organisms for making prothrombin, methods for making the transgenic organisms, methods for making prothrombin-comprising compositions and for further purifying prothrombin from the compositions. Illustrative embodiments of the invention particularly provide transgenic mammals that express an exogenous gene for prothrombin and excrete the prothrombin encoded by the gene into their milk. In a highly particular illustrative embodiment in this regard the invention provides transgenic female pigs that express prothrombin in their milk. In this regard, the invention relates particularly to female pigs having stably incorporated in their genomes a DNA comprising a region that encodes prothrombin operably linked to a mammary gland-specific promoter. Further in this regard the invention relates to the milk containing the prothrombin and to prothrombin-containing compositions derived from the milk.
    Type: Application
    Filed: June 1, 2011
    Publication date: November 17, 2011
    Applicant: Bellweather Farms
    Inventor: William Hugold Velander
  • Patent number: 8030538
    Abstract: The present invention relates to a bovine beta-casein gene targeting vector comprising (1) a first region having a length of 5 to 12 kb which is homologous to the promoter and its flanking nucleic acid sequences of bovine beta-casein gene, and comprising exon 1, intron 1, and exon 2 of bovine beta-casein gene; (2) a region for cloning a nucleic acid coding for desired proteins; (3) a region for coding a positive selection marker; (4) a second region having a length of 2.8 to 3.5 kb which is homologous to the nucleic acid sequences of bovine beta-casein gene, and comprising exon 5, 6, 7 and 8, and intron 5, 6 and 7 of bovine beta-casein gene; wherein the nucleic acid segment corresponding to the first region is located upstream to the nucleic acid segment corresponding to the second region in the 5?-3? arrangement of beta-casein gene.
    Type: Grant
    Filed: November 18, 2005
    Date of Patent: October 4, 2011
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Yong-Mahn Han, Kyung-Kwang Lee, Mira Chang, Deog-Bon Koo
  • Patent number: 8030537
    Abstract: The present invention relates to a method for recombinant manufacturing of substances, wherein cells are transformed suing a nucleotide sequence coding for the substance, the transformed cells are subjected to a cloning process, and the cells obtained in this fashion are introduced into a host organism. The present invention relates in particular to the use of the method in the production of recombinant proteins, cells and tissues. According to a further aspect, the invention relates to a method, wherein the cells of an individual are isolated, said sells are introduced into an immunoincompetent animal for further growth and the cells, tissue and/or organs cultivated in the animal are again isolated and introduced into an individual.
    Type: Grant
    Filed: July 27, 2000
    Date of Patent: October 4, 2011
    Assignee: Apogene GmbH & Co. KG
    Inventor: Gottfried Brem
  • Publication number: 20110239314
    Abstract: The present invention relates to a porcine alpha-S1-casein gene, a porcine alpha-S1-casein gene promoter, an expression comprising the same promoter, and a method for the production of a target protein using the same expression vector. The promoter of the present invention facilitates the mammary gland-specific expression of the target protein. Accordingly, an animal transformed with the promoter secretes the target protein in milk at high concentration, and thus can be advantageously used for the production of useful proteins.
    Type: Application
    Filed: June 29, 2009
    Publication date: September 29, 2011
    Inventors: Myeong Goo Yeo, Sung-Jo Kang, Jong Deok Ahn
  • Publication number: 20110239317
    Abstract: The present invention provides isolated polynucleotides encoding promyostatin polypeptides or a peptide portion thereof, polynucleotides complementary thereto, and oligonucleotides that can specifically hybridize to such polynucleotides. The present invention also provides an isolated polynucleotide encoding a mature myostatin peptide.
    Type: Application
    Filed: January 26, 2011
    Publication date: September 29, 2011
    Inventors: Se-Jin Lee, Alexandra C. McPherron