The Polynucleotide Alters Fat, Fatty Oil, Ester-type Wax, Or Fatty Acid Production In The Plant Patents (Class 800/281)
  • Patent number: 11319545
    Abstract: As a means for artificially inducing functional endosperm in a seed plant without fertilization, provided is a nucleic acid molecule that contains a base sequence encoding a polypeptide capable of inducing endosperm development, said nucleic acid molecule being to be transferred into the genome of the seed plant and expressed therein so as to induce endosperm development in the seed plant without fertilization.
    Type: Grant
    Filed: March 5, 2019
    Date of Patent: May 3, 2022
    Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Masaru Takagi, Miho Ikeda, Nobutaka Mitsuda, Yoshimi Oshima
  • Patent number: 11248244
    Abstract: Provided is a Schizochytrium limacinum strain, a building method therefor and an application thereof. The strain disclosed is classified and named as Schizochytrium sp. HX-RS, and the preservation number is CCTCC NO: M2017046. An acyltransferase functional domain originating from Shewanella PKS enzyme is adopted instead of an acyltransferase functional domain originating from Schizochytrium sp. PKS enzyme, and the strain is obtained by performing flat panel screening and acclimation screening with a high rotation seed and a low temperature.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: February 15, 2022
    Assignee: Nanjing University of Technology
    Inventors: He Huang, Lujing Ren, Shenglan Chen, Lingjun Geng, Xiaojun Ji, Xuechao Hu
  • Patent number: 11242536
    Abstract: Compositions and methods to produce maize with improved nutritional content are disclosed.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: February 8, 2022
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: Joachim Messing, Jose Planta
  • Patent number: 11168367
    Abstract: The disclosure pertains to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to target sequences that flank the target genomic region; elongating the 3? end of the extension probe until the 3? end of the elongated extension probe is adjacent to the 5? end of the ligation probe; and ligating the 3? end of the elongated extension probe with the 5? end of the ligation probe to produce a ligated probe. The ligated probe can be PCR amplified to produce copies of the target genomic region that can be detected or sequenced. Certain embodiments of the invention also provide methods of producing double stranded probes suitable for capturing and analyzing both strands of a target genomic region in a double stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.
    Type: Grant
    Filed: May 29, 2020
    Date of Patent: November 9, 2021
    Assignee: RAPiD Genomics LLC
    Inventors: Leandro Gomide Neves, Adam Payton
  • Patent number: 11162113
    Abstract: The invention provides for an expression cassette comprising a gibberellic acid 2-oxidase gene, and turfgrass plants comprising said cassette.
    Type: Grant
    Filed: December 6, 2019
    Date of Patent: November 2, 2021
    Assignee: OMS INVESTMENTS, INC.
    Inventors: Robert Harriman, Lisa Lee, Rebecca Torisky
  • Patent number: 11041168
    Abstract: The present application discloses an application of OsAO gene for improving rice resistance to rice stripe disease, rice black-streaked dwarf disease or other rice or corn virus diseases caused by homologous virus of rice black-streaked dwarf virus. The present application also provides an application of OsAO gene, the protein encoded by the gene or a recombinant vector containing the gene in regulating plant resistance to rice stripe disease, rice black-streaked dwarf disease or other rice and corn virus diseases caused by homologous virus of rice black-streaked dwarf virus, said protein has the amino acid sequence as shown in Seq 4. The experiment proved that the plant disease resistance is increased in rice overexpressing OsAO gene, indicating that the OsAO protein encoded by this gene plays an important role in rice resistance to rice stripe disease and rice black-streaked dwarf disease.
    Type: Grant
    Filed: August 11, 2016
    Date of Patent: June 22, 2021
    Assignee: Peking University
    Inventors: Yi Li, Xiaofeng Cao, Jianguo Wu, Zhirui Yang, Rongxin Yang, Shengze Yao, Chunhong Wei
  • Patent number: 11041148
    Abstract: The invention relates generally to methods and compositions concerning desaturase enzymes that modulate the number and location of double bonds in long chain poly-unsaturated fatty acids (LC-PUFA's). In particular, the invention relates to methods and compositions for improving omega-3 fatty acid profiles in plant products and parts using desaturase enzymes and nucleic acids encoding for such enzymes. In particular embodiments, the desaturase enzymes are Primula ?6-desaturases. Also provided are improved soybean oil compositions having SDA and a beneficial overall content of omega-3 fatty acids relative to omega-6 fatty acids.
    Type: Grant
    Filed: July 17, 2012
    Date of Patent: June 22, 2021
    Assignee: MONSANTO TECHNOLOGY LLC
    Inventors: Virginia Ursin, Byron Froman, Jennifer Simmons, Thomas J. LaRosa, Fenggao Dong, Steven Screen
  • Patent number: 11015203
    Abstract: The disclosure provides DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The disclosure also provides nucleic acid sequences from Arabidopsis thaliana comprising novel regulatory elements identified from the AtHB17 gene. The disclosure also provides transgenic plants, plant cells, plant parts, and seeds comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.
    Type: Grant
    Filed: May 30, 2019
    Date of Patent: May 25, 2021
    Assignee: Monsanto Technology LLC
    Inventors: Jeffrey E. Ahrens, Paul J. Loida, Elena A. Rice, J. Philip Taylor, Rebecca L. Thompson
  • Patent number: 10975383
    Abstract: Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 552-897 and 6029-10629, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-551 and 898-6028, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.
    Type: Grant
    Filed: August 14, 2018
    Date of Patent: April 13, 2021
    Assignee: Evogene Ltd.
    Inventors: Inbal Nurith Dangoor, Yifat Louba Ofir-Birin, Yaacov Micha Brog, Basia Judith Vinocur, Hagai Karchi
  • Patent number: 10968458
    Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: April 6, 2021
    Inventors: William James Gordon-Kamm, Theodore Mitchell Klein, Keith S. Lowe, Kevin E. McBride, Christopher Jay Scelonge, Ning Wang
  • Patent number: 10900049
    Abstract: The present invention provides an expression cassette comprising a polynucleotide that encodes a protein that diverts a monolignol precursor from a lignin biosynthesis pathway in the plant, which is operably linked to a heterologous promoter. Also provided are methods of engineering a plant having reduced lignin content, as well as plant cells, plant parts, and plant tissues from such engineered plants.
    Type: Grant
    Filed: August 9, 2019
    Date of Patent: January 26, 2021
    Assignee: The Regents of the University of California
    Inventors: Dominique Loque, Aymerick Eudes
  • Patent number: 10900046
    Abstract: The present invention relates to Brassica sequences comprising seed- and funiculus-preferential promoter activity. Provided are recombinant genes comprising the seed- and funiculus-preferential promoter operably linked to a heterologous nucleic acid sequence, and cells, plants and seeds comprising the recombinant gene. The promoters can be used to alter gene expression preferentially in the seeds and in the funiculus and to alter biotic or abiotic stress tolerance, yield, seed quality or seed properties.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: January 26, 2021
    Assignee: BASF AGRICULTURAL SOLUTIONS SEED US LLC
    Inventors: Peter Denolf, Katrien Van Audenhove, John Teske
  • Patent number: 10883113
    Abstract: The present invention provides a method and means to change fatty acid and ultimately triacylglycerol production in plants and algae. Methods of the invention comprise the step of altering the activity levels of the committed step for de novo fatty acid biosynthesis, acetyl-CoA carboxylases (ACCase). More specifically, methods of the invention directly enhance the activity of ACCase by down-regulating the biotin/lipoyl attachment domain containing (BADC) genes through biotechnology or selective breeding approaches.
    Type: Grant
    Filed: July 7, 2016
    Date of Patent: January 5, 2021
    Assignee: The Curators of The University of Missouri
    Inventors: Jay Thelen, Matthew Salie
  • Patent number: 10876127
    Abstract: The present invention relates to recombinant cells, particularly recombinant plant cells, which are capable of producing dihydrosterculic acid and/or derivatives thereof. The present invention also relates to methods of producing oil comprising dihydrosterculic acid and/or derivatives thereof.
    Type: Grant
    Filed: April 12, 2016
    Date of Patent: December 29, 2020
    Assignee: Commonwealth Scientific and Industrial Research Organisation
    Inventors: Craig Christopher Wood, Fatima Naim, Surinder Pal Singh, Shoko Okada
  • Patent number: 10709079
    Abstract: Brassica plants producing oils with a low total saturated fatty acid content and methods for producing such plants are described. The oils have a low total saturated fatty acid in combination with a low, mid, or high oleic acid content.
    Type: Grant
    Filed: April 7, 2016
    Date of Patent: July 14, 2020
    Assignee: Cargill, Incorporated
    Inventors: Honggang Zheng, Kevin Brandt, Richard Fletcher, Daren Coonrod
  • Patent number: 10669554
    Abstract: The invention provides recombinant host organisms (e.g., plants) genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins (e.g., PPTase and/or ACoAS) that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms (e.g., to obtain PUFAs) as well as products obtained from such organisms (e.g., oil and/or seed).
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: June 2, 2020
    Assignees: Dow AgroSciences LLC, DSM IP ASSETS B.V.
    Inventors: Terence A. Walsh, Daniel Gachotte, Ann Owens Merlo, Paul Gordon Roessler, James Metz, Scott Bevan, Jerry Kuner
  • Patent number: 10660293
    Abstract: The invention relates to the soybean variety designated 01077434. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01077434. Also provided by the invention are tissue cultures of the soybean variety 01077434 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01077434 with itself or another soybean variety and plants produced by such methods.
    Type: Grant
    Filed: August 27, 2018
    Date of Patent: May 26, 2020
    Assignee: Monsanto Technology LLC
    Inventor: David R. Wooten, Jr.
  • Patent number: 10660294
    Abstract: The invention relates to the soybean variety designated 01077435. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01077435. Also provided by the invention are tissue cultures of the soybean variety 01077435 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01077435 with itself or another soybean variety and plants produced by such methods.
    Type: Grant
    Filed: August 27, 2018
    Date of Patent: May 26, 2020
    Assignee: Monsanto Technology LLC
    Inventor: David R. Wooten, Jr.
  • Patent number: 10655082
    Abstract: The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
    Type: Grant
    Filed: April 24, 2018
    Date of Patent: May 19, 2020
    Assignees: Commonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corporation, Nuseed Pty Ltd.
    Inventors: James Robertson Petrie, Surinder Pal Singh, Robert Charles de Feyter
  • Patent number: 10570406
    Abstract: Methods for obtaining soybean plants that produce seed with low linolenic acid levels and moderately increased oleic levels are disclosed. Also disclosed are methods for producing seed with low linolenic acid levels, moderately increased oleic levels and low saturated fatty acid levels. These methods entail the combination of transgenes that provide moderate oleic acid levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. These methods also entail the combination of transgenes that provide both moderate oleic acid levels and low saturated fat levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. Soybean plants and seeds produced by these methods are also disclosed.
    Type: Grant
    Filed: December 11, 2017
    Date of Patent: February 25, 2020
    Assignee: Monsanto Technology LLC
    Inventors: Joanne J. Fillatti, Greg E. Keithly, Toni Voelker, Tim Ulmasov
  • Patent number: 10519417
    Abstract: The invention discloses a high-secretion heat-resistant yeast genetically engineered strain and application thereof, belonging to the field of biotechnology. Mutagenesis and domestication are performed to obtain the yeast genetically engineered strain capable of expressing a lipase gene at a high secretion level at high temperature. The strain is collected by China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC NO: M 2016278. The sequencing analysis shows that the lipase gene and promoter sequence thereof are not mutated, which indicates that the high-secretion expression of the lipase gene by the mutant strain is caused by mutation of other gene sequences in the genome.
    Type: Grant
    Filed: July 1, 2016
    Date of Patent: December 31, 2019
    Assignee: Jiangnan University
    Inventors: Xiaowei Yu, Yan Xu
  • Patent number: 10513717
    Abstract: The present invention relates to enzymes which possess desaturase, conjugase, epoxidase and/or hydroxylase activity that can be used in methods of synthesizing fatty acids.
    Type: Grant
    Filed: August 25, 2014
    Date of Patent: December 24, 2019
    Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATION
    Inventors: Katherine Damcevski, Karen Glover, Allan Green, Victoria S. Haritos, Irene Horne, Surinder Pal Singh, Craig C. Wood, Xue-Rong Zhou
  • Patent number: 10492393
    Abstract: Methods for introgressing an allele of interest of a locus associated with a yield trait into Zea mays germplasm are provided. In some embodiments, the methods include providing a Zea mays plant that contains an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by PCR amplification of a Zea mays nucleic acid with a pair of oligonucleotides primers as disclosed herein, and introgressing the allele of interest into Zea mays germplasm that lacks the allele. Also provided are methods for identifying Zea mays plants that contain at least one allele associated with improved yield, improved maize plants, elite Zea mays plants, biomass produced from improved Zea mays plants, isolated and purified genetic markers, and compositions that include an amplification primer pair capable of amplifying a Zea mays nucleic acid to generate a Zea mays marker amplicon.
    Type: Grant
    Filed: June 22, 2018
    Date of Patent: December 3, 2019
    Assignee: Syngenta Participations AG
    Inventors: Venkata Krishna Kishore, Libardo Andres Gutierrez-Rojas, Zhigang Guo, Joseph Byrum
  • Patent number: 10487316
    Abstract: In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: November 26, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Nelson R. Barton, Tim S. Hitchman, Jonathan D. Lyon, Eileen O'Donoghue, Mark A. Wall
  • Patent number: 10472688
    Abstract: The present invention provides compositions and methods for biosynthetically producing podophyllotoxin intermediates and derivatives including enzymes and their equivalents involved in the biosynthetic production of podophyllotoxin intermediates and derivatives.
    Type: Grant
    Filed: August 20, 2016
    Date of Patent: November 12, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Warren Lau, Elizabeth Sattely
  • Patent number: 10443064
    Abstract: A variety of methods and compositions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.
    Type: Grant
    Filed: February 7, 2018
    Date of Patent: October 15, 2019
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: William J. Gordon-Kamm, Keith S. Lowe, David J. Peterson, Christopher J. Scelonge, Grace M. St. Clair, Bing-Bing Wang
  • Patent number: 10415051
    Abstract: Methods and compositions for genetically altered plants that produce higher amounts of at least one fatty acid compared to the amount of the fatty acid produced by a wild-type plant are provided. The genetically altered plant can be a root crop plant (e.g., sugar beet) or Nicotiana spp., or any dicot.
    Type: Grant
    Filed: August 2, 2016
    Date of Patent: September 17, 2019
    Assignees: The United States of America, as Represented by the Secretary of Agriculture, Audacious Energy, LLC
    Inventors: Anna C. Smigocki, Robert Edward Bruccoleri
  • Patent number: 10393744
    Abstract: Provided is a peptide for targeting colorectal cancer, a composition for diagnosing radioresponsiveness-dependent prognosis of colorectal cancer using the peptide, and a drug delivery use of the peptide, wherein a functional peptide capable of targeting cancer has been discovered so as to implement personalized diagnosis and treatment for individual patients having cancer, in consideration of problems occurring during treatment in which treatment cases of respective patients differ due to different therapeutic responses resulting from genetic differences in the individual patients.
    Type: Grant
    Filed: July 29, 2015
    Date of Patent: August 27, 2019
    Assignee: UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION
    Inventors: Eun Kyung Choi, Seong-Yun Jeong, Si Yeol Song, Kyoung Jin Lee, Jinhyang Choi, Jaesook Park, Seok Soon Park, Jae Hee Lee
  • Patent number: 10392626
    Abstract: The disclosure provides DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The disclosure also provides nucleic acid sequences from Arabidopsis thaliana comprising novel regulatory elements identified from the AtHB17 gene. The disclosure also provides transgenic plants, plant cells, plant parts, and seeds comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.
    Type: Grant
    Filed: October 9, 2014
    Date of Patent: August 27, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Jeffrey E. Ahrens, Paul J. Loida, Elena A. Rice, J. Philip Taylor, Rebecca L. Thompson
  • Patent number: 10392629
    Abstract: As described herein, plants expressing algal Diacylglycerol Acyltransferase Type Two (DGTT) from heterologous nucleic acids can alter acyl carbon partitioning in plant vegetative tissues and increase acyl-CoA-dependent triacylglycerol synthesis, thereby increasing the lipid content of the plants' tissues.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: August 27, 2019
    Assignee: Board of Trustees of Michigan State University
    Inventors: Christoph Benning, Sanjaya, Rachel Miller
  • Patent number: 10351869
    Abstract: The application provides recombinant microorganisms with increased productivity with respect to control or wildtype microorganisms. The recombinant microorganisms can include a non-native gene encoding a SKP1 polypeptide or a CHORD-derived polypeptide. Increased productivity can be increased biomass or lipid productivity. These recombinant microorganisms can be used to produce products of interest.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: July 16, 2019
    Assignee: Synthetic Genomics, Inc.
    Inventors: Nicholas Bauman, Imad Ajjawi
  • Patent number: 10337038
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.
    Type: Grant
    Filed: July 18, 2014
    Date of Patent: July 2, 2019
    Assignee: CARGILL, INCORPORATED
    Inventors: Michael Lynch, Michael Louie, Shelley Copley, Eileen Spindler, Brittany Prather, Matthew Lipscomb, Tanya Lipscomb, Hans Liao, Dave Hogsett, Ron Evans
  • Patent number: 10308962
    Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of unsaturated C6-C24 fatty alcohols, aldehydes, and acetates which may be useful as insect pheromones, fragrances, flavors, and polymer intermediates. The C6-C24 fatty alcohols, aldehydes, and acetates described herein may be used as substrates for metathesis reactions to expand the repertoire of target compounds and pheromones. The application further relates to recombinant microorganisms co-expressing a pheromone pathway and a pathway for the production of a toxic protein, peptide, oligonucleotide, or small molecule suitable for use in an attract-and-kill pest control approach. Also provided are methods of producing unsaturated C6-C24 fatty alcohols, aldehydes, and acetates using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally one or more of the product alcohols, aldehydes, or acetates.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: June 4, 2019
    Assignee: Provivi, Inc.
    Inventors: Effendi Leonard, Peter Meinhold, Keith Wampler, Pedro Coelho, Micah Sheppard, Thomas Heel
  • Patent number: 10294509
    Abstract: To provide a method for simply measuring ethanolamine phosphate in a sample, and a reagent, kit, program and the like useful in the method. A measurement method of ethanolamine phosphate includes a first step of adding an enzyme, which can catalyze a reaction that forms acetaldehyde from ethanolamine phosphate, to a sample, and conducting a first enzymatic reaction to form acetaldehyde, phosphoric acid and ammonia; and a second step of quantifying at least one of the resultant acetaldehyde, phosphoric acid and ammonia to determine an amount of the ethanolamine phosphate in the measurement sample.
    Type: Grant
    Filed: March 10, 2017
    Date of Patent: May 21, 2019
    Assignee: HUMAN METABOLOME TECHNOLOGIES, INC.
    Inventors: Takushi Ohga, Yoshiaki Ohashi
  • Patent number: 10280431
    Abstract: Provided herein are novel acyltransferases and methods of using such novel acyltransferases in making medium-chain fatty acids.
    Type: Grant
    Filed: December 18, 2014
    Date of Patent: May 7, 2019
    Assignee: NUtech Ventures
    Inventors: Edgar Cahoon, Umidjon Iskandarov, Hae Jin Kim, Jillian Collins-Silva
  • Patent number: 10260077
    Abstract: Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2) and increasing the activity of a type 2 diacylglycerol acyltransferase (i.e., DGA1). In some embodiments, the triacylglycerol content of a cell is also modified by decreasing the activity of a triacylglycerol lipase in the same cell. Also disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2), or by increasing the activity of a type 3 diacylglycerol acyltransferase (i.e., DGA3).
    Type: Grant
    Filed: May 29, 2015
    Date of Patent: April 16, 2019
    Assignee: Novogy, Inc.
    Inventors: Vasiliki Tsakraklides, Elena E. Brevnova
  • Patent number: 10260073
    Abstract: Provided are isolated polynucleotides and nucleic acid constructs which comprise a nucleic acid sequence at least 80% identical to a nucleic acid sequence selected form the group consisting of SEQ ID NOs: 1-219, 367-5628, 9688-9700, and 9709-9752; and isolated polypeptides which comprise an amino acid sequence at least 80% homologous to an amino acid sequence selected from the group consisting of SEQ ID NOs: 220-366, 5629-9400, 9701-9708, and 9753-9796. Also provided are transgenic cells and plants expressing same and methods of using same for increasing yield, growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiber quality, nitrogen use efficiency, and/or abiotic stress of a plant.
    Type: Grant
    Filed: December 26, 2012
    Date of Patent: April 16, 2019
    Assignee: Evogene Ltd.
    Inventors: Avital Adato, Hagai Karchi
  • Patent number: 10253325
    Abstract: In some embodiments, the present invention provides a method of elevating lipid content in vegetative (non-seed) plant or algae cells, plant tissues, or whole plants by genetically modifying the plant or algae to express a lipid droplet-associated protein or polypeptide (such as fat-specific protein 27) of mammalian origin. Also provided are genetically-modified plant or algae cells, plant tissues, or whole plants with elevated cellular lipid content, expressing a lipid droplet-associated protein or polypeptide (such as fat-specific protein 27) of mammalian (e.g. human) origin.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: April 9, 2019
    Assignee: BOSTON MEDICAL CENTER CORPORATION
    Inventors: Vishwajeet Puri, Kent Chapman, Christopher James
  • Patent number: 10184128
    Abstract: The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5?-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-?C7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides.
    Type: Grant
    Filed: July 14, 2016
    Date of Patent: January 22, 2019
    Assignee: ACADEMIA SINICA
    Inventors: Hsou-Min Li, Chiung-Chih Chu
  • Patent number: 10100321
    Abstract: The present invention relates to the field of plant molecular biology, more particularly cotton WRINKLED 1-like (WREL) genes. More specifically, the present invention relates to cotton WRIL genes whose products act as transcription factors of genes involved in fatty acid biosynthesis. The present invention also relates to methods of increasing cotton fiber length in cotton. In one embodiment, the methods involve modulating the level of activity of an enzyme involved in a fatty acid biosynthesis in the host cotton cell and/or culturing the host cotton cell. In another embodiment, the methods involve the manipulation of transcription factors which can regulate an enzyme involved in fatty acid biosynthesis.
    Type: Grant
    Filed: April 19, 2012
    Date of Patent: October 16, 2018
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Nam-Hai Chua, Jian Ye, Jing Qu
  • Patent number: 10036030
    Abstract: Recombinant DNA constructs comprising the soybean sucrose synthase promoter operably linked to polynucleotides encoding transcription factors such as ODP1, Lec1 and FUSCA3 are disclosed. These constructs are used for increasing oil content while maintaining normal germination in oilseed plants. Methods to increase oil content in the seeds of an oilseed plant using this construct are also disclosed herein.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: July 31, 2018
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Howard Glenn Damude, Bryce Daines, Knut Meyer, Kevin G Ripp, Kevin L Stecca
  • Patent number: 10030215
    Abstract: Provided are isolated polypeptides with lipase activity. Also provided are polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the polynucleotides and methods of using the polypeptides or the compositions.
    Type: Grant
    Filed: April 13, 2015
    Date of Patent: July 24, 2018
    Assignee: Novozymes A/S
    Inventors: Ming Li, Tianqi Sun, Xianzhi Jiang, Han Bucong, Carsten Hoerslev Hansen, Kim Borch, Lone Baunsgaard
  • Patent number: 9982273
    Abstract: Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention have been shown to confer at least one regulatory activity and confer increased yield, greater height, greater early season growth, greater canopy coverage, greater stem diameter, greater late season vigor, increased secondary rooting, more rapid germination, greater cold tolerance, greater tolerance to water deprivation, reduced stomatal conductance, altered C/N sensing, increased low nitrogen tolerance, increased low phosphorus tolerance, or increased tolerance to hyperosmotic stress as compared to the control plant as compared to a control plant.
    Type: Grant
    Filed: January 21, 2015
    Date of Patent: May 29, 2018
    Assignees: Mendel Biotechnology, Inc, Monsanto Technology LLC
    Inventors: Robert A. Creelman, Neal I. Gutterson, Oliver J. Ratcliffe, T. Lynne Reuber, Richard Eric Cerny, Kimberly Faye Zobrist Duff, Susanne Kjemtrup-Lovelace, Robert J. Meister, Marie E. Petracek, Thomas Ruff, Qingzhang Xu
  • Patent number: 9976155
    Abstract: Compositions and methods include genetically encoding and expressing a novel ?9-18:0-ACP desaturase in plant cells. In some embodiments, nucleic acid molecules encode the novel ?9-18:0-ACP desaturase. In other embodiments, amino acid sequences have ?9-18:0-ACP desaturase activity. Methods can involve expression of ?9-18:0-ACP desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: May 22, 2018
    Assignees: Dow AgroSciences LLC, Brookhaven Science Associates, LLC
    Inventors: John Shanklin, Tam Huu Nguyen, Terence A. Walsh, Mark S. Pidkowich, Edward J. Whittle
  • Patent number: 9938486
    Abstract: The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.
    Type: Grant
    Filed: July 15, 2014
    Date of Patent: April 10, 2018
    Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATION
    Inventors: James Robertson Petrie, Anne Maree Mackenzie, Qing Liu, Pushkar Shrestha, Peter David Nichols, Susan Irene Ellis Blackburn, Maged Peter Mansour, Stanley Suresh Robert, Dion Matthew Frederick Frampton, Xue-Rong Zhou, Surinder Pal Singh, Craig Christopher Wood
  • Patent number: 9932289
    Abstract: The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
    Type: Grant
    Filed: January 6, 2017
    Date of Patent: April 3, 2018
    Assignees: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH OGRANISATION, GRAINS RESEARCH AND DEVELOPMENT CORPORATION, NUSEED PTY LTD
    Inventors: James Robertson Petrie, Surinder Pal Singh, Robert Charles de Feyter
  • Patent number: 9926539
    Abstract: Two novel cytochrome P450 genes are isolated from sorghum, each gene encoding a protein having pentadecatrienyl resorcinol hydroxylase activity. Expression vectors containing these sequences are made and used to elevate levels of pentadecatrienyl resorcinol hydroxylase in transgenic cells and organisms.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: March 27, 2018
    Assignee: The United States of America, as represented by The Secretary of Agriculture
    Inventors: Zhiqiang Pan, Scott Baerson
  • Patent number: 9914932
    Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.
    Type: Grant
    Filed: November 13, 2014
    Date of Patent: March 13, 2018
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Jon K. Magnuson, Shuang Deng, Kenneth S. Bruno, David E. Culley
  • Patent number: 9914930
    Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
    Type: Grant
    Filed: September 5, 2013
    Date of Patent: March 13, 2018
    Assignees: Dow AgroSciences LLC, Sangamo Therapeutics, Inc.
    Inventors: Noel Cogan, John Forster, Matthew Hayden, Tim Sawbridge, German Spangenberg, Steven R. Webb, Manju Gupta, William Michael Ainley, Matthew J. Henry, Jeffrey C. Miller, Dmitry Y. Guschin
  • Patent number: 9873887
    Abstract: Methods for obtaining soybean plants that produce seed with low linolenic acid levels and moderately increased oleic levels are disclosed. Also disclosed are methods for producing seed with low linolenic acid levels, moderately increased oleic levels and low saturated fatty acid levels. These methods entail the combination of transgenes that provide moderate oleic acid levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. These methods also entail the combination of transgenes that provide both moderate oleic acid levels and low saturated fat levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. Soybean plants and seeds produced by these methods are also disclosed.
    Type: Grant
    Filed: January 7, 2016
    Date of Patent: January 23, 2018
    Assignee: Monsanto Technology LLC
    Inventors: Joanne J. Fillatti, Greg E. Keithly, Toni Voelker, Tim Ulmasov