The Polynucleotide Contains A Tissue, Organ, Or Cell Specific Promoter Patents (Class 800/287)
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Patent number: 8847016Abstract: The invention provides several promoters isolated from Oryza sativa, which promoters are capable of driving and/or regulating the expression of an operably linked nucleic acid in a plant. The expression patterns of the promoters according to the invention have been studied in Oryza sativa and some of the promoters displayed specific activity in particular cells, tissues or organs of the plant, while others displayed constitutive expression throughout substantially the whole plant. Some promoters showed weak expression, while others were strongly active.Type: GrantFiled: May 15, 2012Date of Patent: September 30, 2014Assignee: Crop Design N.V.Inventors: Yves Hatzfeld, Willem Broekaert
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Patent number: 8847015Abstract: The invention provides several promoters isolated from Oryza sativa, which promoters are capable of driving and/or regulating the expression of an operably linked nucleic acid in a plant. The expression patterns of the promoters according to the invention have been studied in Oryza sativa and some of the promoters displayed specific activity in particular cells, tissues or organs of the plant, while others displayed constitutive expression throughout substantially the whole plant. Some promoters showed weak expression, while others were strongly active.Type: GrantFiled: August 20, 2008Date of Patent: September 30, 2014Assignee: Cropdesign N.V.Inventors: Yves Hatzfeld, Willem Broekaert
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Publication number: 20140283205Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding Sorghum bicolor TIP2-3. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.Type: ApplicationFiled: March 12, 2013Publication date: September 18, 2014Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andrew C. Crow, Scott Diehn, Brooke Peterson-Burch
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Publication number: 20140283206Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding Sorghum bicolor pLTP. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.Type: ApplicationFiled: March 12, 2013Publication date: September 18, 2014Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andrew C. Crow, Scott Diehn, Brooke Peterson-Burch
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Patent number: 8829275Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.Type: GrantFiled: July 25, 2012Date of Patent: September 9, 2014Assignee: Monsanto Technology LLCInventors: Xudong Ye, Michael W. Petersen, Larry Gilbertson, David Walters, Susan Johnson, Shihshieh Huang, Paul S. Chomet
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Publication number: 20140250548Abstract: Provided is a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an NEMTOP6 polypeptide. Also provided are plants having modulated expression of a nucleic acid encoding an NEMTOP6 polypeptide, which plants have enhanced yield-related traits compared with control plants. Also provided are NEMTOP6-encoding nucleic acids, and constructs comprising the same, useful in enhancing yield-related traits in plants.Type: ApplicationFiled: April 13, 2012Publication date: September 4, 2014Applicant: BASF Plant Science Company GmbH Patents, Trademarks and LicensesInventors: Christophe Reuzeau, Steven Vandenabeele
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Patent number: 8822759Abstract: The present invention relates to transcriptional control sequences derived from GL9 genes, wherein the transcriptional control sequences direct specific or preferential expression of an operably connected nucleotide sequence of interest in one or more parts of a plant seed.Type: GrantFiled: March 18, 2011Date of Patent: September 2, 2014Assignee: Australian Centre for Plant Functional Genomics Pty. Ltd.Inventors: Sergiy Lopato, Nataliya Kovalchuk
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Patent number: 8809626Abstract: Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising an trichome specific promoter and methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.Type: GrantFiled: December 16, 2008Date of Patent: August 19, 2014Assignee: KeyGene N.V.Inventors: Petronella Martina Bleeker, Christianus Cornelis Nicolaas Van Schie, Paul Johan Diergaarde, Robert Cornelis Schuurink, Michel Albertus Haring, Michiel Theodoor Jan De Both
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Patent number: 8809630Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.Type: GrantFiled: November 26, 2007Date of Patent: August 19, 2014Assignee: Mendel Biotechnology, Inc.Inventors: Roderick W. Kumimoto, Luc J. Adam, Roger Canales, Karen S. Century, Robert A. Creelman, Neal I. Gutterson, Frederick D. Hempel, Jacqueline E. Heard, Cai-Zhong Jiang, Katherine Krolikowski, Omaira Pineda, Oliver J. Ratcliffe, Peter P. Repetti, T. Lynne Reuber
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Publication number: 20140230093Abstract: The present disclosure provides compositions and methods for regulating expression of transcribable polynucleotides in plant cells, plant tissues, and plants. Compositions include regulatory polynucleotide molecules capable of providing expression in plant tissues and plants. Methods for expressing polynucleotides in a plant cell, plant tissue, or plants using the regulatory polynucleotide molecules disclosed herein are also provided.Type: ApplicationFiled: July 18, 2012Publication date: August 14, 2014Inventors: Tedd D. Elich, Philip N. Benfey, Ian Davis, David A. Orlando
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Patent number: 8802929Abstract: A rice microRNA, miR164 gene, that controls plant root system development and fertility, is obtained through gene isolation, cloning and function verification. Uses of a nucleic acid fragment comprising miR164, which fragment may confer a transformed plant with the ability to increase root number and to alter fertility, wherein the said nucleic acid fragment is selected from one of the following nucleotide sequences: 1) a DNA sequence as shown in SEQ ID NO: 1; 2) a RNA sequence as shown in SEQ ID NO:2; or 3) the conserved sequence of miR164 having the same function as 1) or 2). The nucleotide sequence containing the precursor of miR164 is ligated with an exogenous promoter and introduced into rice to obtain transgenic rice plants which has large root systems but became infertile. The fertility can be restored by external application of phytohormones.Type: GrantFiled: May 12, 2010Date of Patent: August 12, 2014Assignee: Huazhong Agricultural UniversityInventors: Lizhong Xiong, Kabin Xie, Xin Hou
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Publication number: 20140223603Abstract: The invention relates to transgenic plants with improved growth and nitrogen use efficiency expressing nitrate transporter gene, methods of making such plants and methods for improving growth and nitrogen use efficiency.Type: ApplicationFiled: February 4, 2014Publication date: August 7, 2014Applicants: Plant Bioscience Limited, Nanjing Agricultural UniversityInventors: Guohua Xu, Xiaorong Fan, Qirong Shen, Anthony Miller
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Patent number: 8796507Abstract: The present invention relates to promoters that enable gene expression which is both specific to the endosperm and early during the development of the endosperm, as well as nucleic acid molecules encoding basal endosperm transfer cell layer (BETL) proteins.Type: GrantFiled: April 1, 2011Date of Patent: August 5, 2014Assignee: BiogemmaInventors: Pascual Perez, José Gutierrez-Marcos, Hugh Dickinson
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Patent number: 8796510Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.Type: GrantFiled: February 6, 2012Date of Patent: August 5, 2014Assignee: Mendel Biotechnology, Inc.Inventors: Jacqueline E. Heard, Robert A. Creelman, Oliver J. Ratcliffe, T. Lynne Reuber, James S. Keddie
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Publication number: 20140215655Abstract: In one aspect, the application discloses a cotton plant cell comprising (a) a chimeric gene comprising a first nucleic acid sequence comprising at least 700 consecutive nucleotides of SEQ ID NO: 1 or a nucleic acid sequence having at least 80% sequence identity thereto any of which has stomata-preferential promoter activity; (b) a second nucleic acid sequence encoding an expression product of interest; and (c) a transcription termination and polyadenylation sequence. In addition, the present application discloses a cotton plant, a method of expressing a transgene in cotton under stress conditions, a method of producing a cotton plant, a method of detecting the expression of a transgene under stress conditions and a method for modulating the resistance of a cotton plant to stress as characterized in the claims.Type: ApplicationFiled: August 9, 2012Publication date: July 31, 2014Applicant: BAYER CROPSCIENCE NVInventors: Stéphane Pien, Bart Den Boer
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Patent number: 8791330Abstract: The present invention relates generally to an expression regulatory element operable in plants which includes oil palm, and in particular to an expression regulatory element operable selectively in the endosperm tissue of the plants. Said expression regulatory element comprises a sequence of nucleotides which specifically modulates the expression of a second nucleic acid molecule operably coupled to said expression regulatory element.Type: GrantFiled: March 13, 2008Date of Patent: July 29, 2014Assignee: Malaysian Palm Oil BoardInventors: Siti Nor Akmar Abdullah, Suan Choo Cheah, Nurniwalis Abd. Wahab
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Patent number: 8785722Abstract: Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, methods for testing allelic variants, and methods for repressing transmission of transgenes.Type: GrantFiled: October 19, 2010Date of Patent: July 22, 2014Assignee: Pioneer Hi Bred International IncInventors: Andrew Mark Cigan, Erica Unger
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Publication number: 20140201866Abstract: The present invention provides methods and compositions for increasing the seed size and/or seed number in plants. In particular, the methods and compositions provide for the over expression of a plant growth and/or development related or associated gene during embryo development. Transgenic plants transformed with genetic constructs having the plant growth and/or development associated gene under the control of an early phase-specific embryo promoter provides mature plants in the field that produce larger and/or more seeds. Methods for selection growth and development associated genes that provide transgenic plants with a higher yield phenotype are also provided.Type: ApplicationFiled: January 7, 2014Publication date: July 17, 2014Applicant: TARGETED GROWTH, INC.Inventors: JAY DEROCHER, THU NGUYEN
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Publication number: 20140201864Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.Type: ApplicationFiled: February 11, 2014Publication date: July 17, 2014Applicant: MENDEL BIOTECHNOLOGY, INC.Inventors: Roderick W. Kumimoto, Luc J. Adam, Roger Canales, Karen S. Century, Robert A. Creelman, Jennifer M. Costa, Neal I. Gutterson, Frederick D. Hempel, Jacqueline E. Heard, Cai-Zhong Jiang, Katherine Krolikowski, Omaira Pineda, Emily L. Queen Kumimoto, Oliver J. Ratcliffe, Peter P. Repetti, T. Lynne Reuber, Jose Luis Riechmann, James Z. Zhang
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Publication number: 20140201861Abstract: The present description relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant, including the traits of increased yield, size or biomass.Type: ApplicationFiled: January 16, 2014Publication date: July 17, 2014Applicant: Mendel Biotechnology, Inc.Inventors: Jose Luis Riechmann, Oliver J Ratcliffe, Jacqueline E Heard, T. Lynne Reuber
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Patent number: 8778672Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for an Arabidopsis thaliana nucellar 1/inner integument (AT NUC1) promoter. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.Type: GrantFiled: October 26, 2010Date of Patent: July 15, 2014Assignee: Pioneer Hi Bred International IncInventors: Shai Lawit, Mark Chamberlin
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Patent number: 8779238Abstract: The present invention provides methods for transforming Camelina plants. In particular, the present invention relates to transforming Camelina sativa plants through contacting the plants to a dipping solution comprising Agrobacterium, a sugar, and a nonionic surfactant. The methods do not require a vacuum filtration step. The present invention provides, for example, useful methods for developing transformation systems for Camelina sativa that can enable manipulation of its agronomic qualities.Type: GrantFiled: March 19, 2009Date of Patent: July 15, 2014Assignee: Global Clean Energy Holdings, Inc.Inventors: Thu Nguyen, Xunjia Liu, Jay Derocher
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Publication number: 20140196176Abstract: The invention provides methods for isolating cell-type specific mRNAs by selectively isolating ribosomes or proteins that bind mRNA in a cell type specific manner, and, thereby, the mRNA hound to the ribosomes or proteins that bind mRNA. Ribosomes, which are riboprotein complexes, bind mRNA that is being actively translated in cells. According to the methods of the invention, cells are engineered to express a molecularly tagged ribosomal protein or protein that binds mRNA by introducing into the cell a nucleic acid comprising a nucleotide sequence encoding a ribosomal protein or protein that binds mRNA fused to a nucleotide sequence encoding a peptide tag. The tagged ribosome or mRNA binding protein can then be isolated, along with the mRNA bound to the tagged ribosome or mRNA binding protein, and the mRNA isolated and further used for gene expression analysis.Type: ApplicationFiled: June 28, 2013Publication date: July 10, 2014Inventors: Nathaniel Heintz, Tito A. Serafini, Andrew W. Shyjan
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Patent number: 8765929Abstract: The objective of the present invention is to provide a transformation method that is applicable to a wide variety of species of algae with high efficiency. The promoter of the present invention is characterized in containing a polynucleotide constituting a non-coding region located upstream from a gene encoding a replication-associated protein of a CdebDNA virus or the like.Type: GrantFiled: January 22, 2010Date of Patent: July 1, 2014Assignee: Kochi UniversityInventors: Masao Adachi, Keizo Nagasaki, Yuji Tomaru
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Patent number: 8766038Abstract: The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology.Type: GrantFiled: May 16, 2007Date of Patent: July 1, 2014Inventors: Thomas Schmulling, Tomas Werner
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Patent number: 8759612Abstract: The invention relates to gene expression regulatory sequences from soybean, specifically to the promoters of a soybean lipid transfer protein LTP2 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The described LTP2 promoters preferentially initiate transcription in flower cells. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: GrantFiled: May 14, 2008Date of Patent: June 24, 2014Assignee: E. I. du Pont de Nemours and CompanyInventor: Zhongsen Li
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Patent number: 8759506Abstract: The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.Type: GrantFiled: September 23, 2011Date of Patent: June 24, 2014Assignee: BASF Plant Science GmbHInventors: Hee-Sook Song, Christian Dammann, Marc Morra, Jeffrey A. Brown, Liqun Xing, Hongmei Jia
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Patent number: 8759632Abstract: The present invention provides isolated nucleic acids comprising nucleotide sequences encoding isoprenoid modifying enzymes, as well as recombinant vectors comprising the nucleic acids. The present invention further provides genetically modified host cells comprising a subject nucleic acid or recombinant vector. The present invention further provides a transgenic plant comprising a subject nucleic acid. The present invention further provides methods of producing an isoprenoid compound, the method generally involving culturing a subject genetically modified host cell under conditions that permit synthesis of an isoprenoid compound modifying enzyme encoded by a subject nucleic acid.Type: GrantFiled: March 21, 2012Date of Patent: June 24, 2014Assignee: The Regents of the University of CaliforniaInventors: Dae-Kyun Ro, Karyn Newman, Eric M. Paradise, Jay D. Keasling, Mario Ouellet, Rachel Eachus, Kimberly Ho, Timothy Ham
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Publication number: 20140173779Abstract: The invention provides nucleotide sequences that can be used in operable association with a promoter to express a polynucleotide sequence of interest in a plant, plant part or plant cell at particular stages of development and/or in specific tissues. and methods for directing developmental stage specific and/or tissue specific expression of a polynucleotide of interest.Type: ApplicationFiled: April 5, 2013Publication date: June 19, 2014Applicants: The University of Guelph, Syngenta Participations AGInventors: Christophe Liseron-Monfils, Manish N. Raizada, Joseph Colasanti, Gregory Downs, Yong-Mei Bi, Lewis N. Lukens, Steven J. Rothstein, Tong Zhu, Xi Chen
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Patent number: 8754292Abstract: Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.Type: GrantFiled: March 9, 2009Date of Patent: June 17, 2014Assignee: Pioneer Hi Bred International IncInventors: Marc Albertsen, Tim Fox, Gary Huffman, Mary Trimnell
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Patent number: 8754200Abstract: The present invention relates to promoters that enable gene expression which is both specific to the endosperm and early during the development of the endosperm, as well as nucleic acid molecules encoding basal endosperm transfer cell layer (BETL) proteins.Type: GrantFiled: June 8, 2010Date of Patent: June 17, 2014Assignee: BiogemmaInventors: Pascual Perez, José Gutierrez-Marcos, Hugh Dickinson
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Patent number: 8742201Abstract: The present invention relates to a nitrogen-regulated GATA transcription factor gene required for sugar sensing and the modulation of the expression of this gene to modulate a characteristic in a plant. The GATA transcription factor of the present invention is involved in regulating sugar sensing in plants and its expression is influenced by nitrogen status. Increased expression of this or substantially similar genes can produce plants with improved nitrogen utilization and increased yield.Type: GrantFiled: May 6, 2010Date of Patent: June 3, 2014Assignee: University of GuelphInventors: Steven Rothstein, Yong-Mei Bi
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Patent number: 8742205Abstract: Disclosed are mutant CDK inhibitor (CKI) polypeptides having dominant negative antagonist activity against wild-type CKI proteins, as well as related compositions, including nucleic acids and vectors encoding the mutant CKI polypeptides and transformed host cells and transgenic plants comprising such nucleic acids and vectors. Also disclosed are related methods for using the mutant proteins to modulate cell division in cells, particularly plant cells.Type: GrantFiled: July 28, 2006Date of Patent: June 3, 2014Assignee: Targeted Growth, Inc.Inventors: Paul Olivier, Jay DeRocher, James M. Roberts
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Patent number: 8735655Abstract: The present invention relates to DNA polynucleotides for regulating gene expression in plants. In particular, the invention relates to 5? regulatory sequences isolated from Zea mays that are useful for regulating gene expression of heterologous DNA molecules in plant roots. The invention also relates to transgenic plants containing the heterologous DNA molecules.Type: GrantFiled: November 19, 2009Date of Patent: May 27, 2014Assignee: Monsanto Technology LLCInventors: Heather M. Anderson, Timothy W. Conner, Colleen G. Santino
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Patent number: 8729339Abstract: The invention provides methods and compositions useful in target sequence suppression and target sequence validation. The invention provides polynucleotide constructs useful for gene silencing, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence.Type: GrantFiled: December 18, 2007Date of Patent: May 20, 2014Assignee: E.I du Pont de Nemours and CompanyInventor: Milo J. Aukerman
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Patent number: 8729340Abstract: The present invention relates to a Solanum lycopersicum histidine decarboxylase-2 (SlHD-2) gene-derived fruit-specific expression promoter, to a 5? untranslated region (5?-UTR), to a (5?UTR), —specific expression vector comprising same, to a method for the fruit-specific expression of an exotic gene using the expression vector, to a plant transformed with the expression vector, and to a seed thereof. According to the present invention, a gene introduced from a transformed plant can be specifically expressed fruit tissue as compared to the widely used conventional Cauliflower mosaic virus-derived CaMV 35S promoter which induces the expression of an exotic gene in the whole tissue. Further, the present invention can be valuably used in the development of a transformed plant, which seeks to produce valuable substances from fruit.Type: GrantFiled: February 11, 2010Date of Patent: May 20, 2014Assignee: Industry Foundation of Chonnam National UniversityInventors: Young Hee Joung, Ah Young Kim, Sang Hyeob Lee
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Publication number: 20140137277Abstract: Polynucleotides and polypeptides incorporated into expression vectors are introduced into plants and were ectopically expressed. These polypeptides may confer at least one regulatory activity and increased photosynthetic resource use efficiency, increased yield, greater vigor, greater biomass as compared to a control plant.Type: ApplicationFiled: March 13, 2013Publication date: May 15, 2014Applicant: Mendel Biotechnology, Inc.Inventors: Graham J. Hymus, Colleen M. Marion, T. Lynne Reuber, Oliver J. Ratcliffe
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Patent number: 8722967Abstract: The present invention relates to methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase in plants or plant parts, preferably in seeds, embryos and/or cotyledons. The methods result in increases in: yield, growth of seedlings, early vigor, and stress tolerance. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences.Type: GrantFiled: May 11, 2012Date of Patent: May 13, 2014Inventors: Thomas Schmulling, Tomas Werner
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Patent number: 8710301Abstract: The present invention relates to a method for producing male sterile plants. It involves selective killing of reproductive cells in plants by using an autophagy related gene plant BECLIN I/ATG6. An expression cassette comprising plant BECLIN I/ATG6, regulated by a tapetum specific promoter can induce killing of tapetum cells. A particular area of interest is transforming a plant with said genetic construct and expression of the plant BECLIN I/ATG6 gene in tapetum at early stage of anther development to cause early collapse of tapetum to produce nonviable pollen, thus imparting male sterility in plants.Type: GrantFiled: November 26, 2009Date of Patent: April 29, 2014Assignee: Council of Scientific & Industrial ResearchInventors: Samir Vishwanath Sawant, Rakesh Tuli, Sudhir Pratap Singh
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Patent number: 8709811Abstract: The subject invention concerns polynucleotides encoding a plant 2-phenylethanol dehydrogenase enzyme. In one embodiment, the polynucleotide encodes a tomato 2-phenylethanol dehydrogenase. The subject invention also concerns polynucleotides encoding a plant phenylalanine decarboxylase enzyme. In one embodiment, the polynucleotide encodes a tomato phenylalanine decarboxylase. The subject invention also concerns 2-phenylethanol dehydrogenase polypeptides and phenylalanine decarboxylase polypeptides encoded by polynucleotides of the present invention. The subject invention also concerns methods for providing a plant with an increased flavor and aroma volatile. Plants can be transformed with one or more polynucleotide of the present invention. The subject invention also concerns these transformed plant cells, plant tissue, and plants and transgenic progeny thereof.Type: GrantFiled: October 1, 2004Date of Patent: April 29, 2014Assignee: University of Florida Research Foundation, Inc.Inventors: Harry J. Klee, Denise Tieman
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Patent number: 8710206Abstract: The promoter of a soybean translation elongation factor EF1 alpha, a polypeptide that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis, and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants are described.Type: GrantFiled: May 14, 2008Date of Patent: April 29, 2014Assignee: E. I. du Pont de Nemours and CompanyInventor: Zhongsen Li
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Patent number: 8710297Abstract: A glycosyltransferase promoter and a recombinant nucleic acid, plant cell and transgenic plant containing thereof are provided. The promoter includes a nucleotide sequence as set forth in any one of SEQ ID NOs: 1˜7, a fragment having at least 10 contiguous bases of any one of SEQ ID NOs: 1˜7 or a combination thereof, or a nucleotide sequence having 90% or more identity to the nucleotide sequence as set forth in any one of SEQ ID NOs: 1˜7.Type: GrantFiled: July 21, 2011Date of Patent: April 29, 2014Assignee: Industrial Technology Research InstituteInventors: Shu-Jiau Chiou, Cheng-Yu Lee, Pei-Hsuan Wei, Jui-Hung Yen, Cheng-Li Fang
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Patent number: 8704040Abstract: Provided is an isolated polypeptide having triterpene methyltransferase activity. Also provided is an isolated nucleic acid molecule that encodes the triterpene methyltransferase polypeptides; a vector comprising the nucleic acid molecules that encode the triterpene methyltransferase polypeptides; and a host cell(s) transfected with the aforementioned nucleic acid molecule or vector. In another aspect, a method of producing a methylated triterpene is provided. The method comprises providing a metabolizable carbon source to a host cell transfected with a nucleic acid molecule that encodes a triterpene methyltransferase under conditions sufficient for production of a methylated triterpene. The method optionally further comprises isolating the methylated triterpene produced by the host cell.Type: GrantFiled: June 22, 2009Date of Patent: April 22, 2014Assignee: University of Kentucky Research FoundationInventors: Joseph Chappell, Shigeru Okada, Scott Kinison, Tom Niehaus
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Patent number: 8704041Abstract: A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.Type: GrantFiled: December 30, 2010Date of Patent: April 22, 2014Assignee: Pioneer Hi Bred International IncInventors: William J. Gordon-Kamm, Keith S. Lowe, David J. Peterson, Christopher J. Scelonge, Grace M. St. Clair, Bing-Bing Wang
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Patent number: 8697857Abstract: The promoter of a soybean translation elongation factor EF1 alpha, a polypeptide that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis, and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants are described.Type: GrantFiled: November 20, 2008Date of Patent: April 15, 2014Assignee: E. I. du Pont de Nemours and CompanyInventor: Zhongsen Li
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Patent number: 8697949Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.Type: GrantFiled: June 26, 2008Date of Patent: April 15, 2014Assignee: Monsanto Technology LLCInventors: Edwards Allen, Sara E. Heisel, Sergey Ivashuta, Elysia Krieger, Jennifer Lutke, Robert J. Meister, Yuanji Zhang
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Patent number: 8691962Abstract: The invention provides isolated plant gene promoters and regulatory elements that are root specific and/or induced by plant parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots and are particularly useful for controlling transcription of nucleic acids encoding agents that disrupt formation or maintenance of parasitic nematode feeding sites in plants.Type: GrantFiled: December 20, 2007Date of Patent: April 8, 2014Assignee: BASF Plant Science GmbHInventors: Aaron Wiig, Robert Ascenzi, Xiang Huang, Sumita Chaudhuri, Rui-Guang Zhen, Yu Han
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Patent number: 8692068Abstract: The present invention relates to polynucleotide molecules for regulating gene expression in plants. In particular, the present invention relates to promoters isolated from Brassica napus that are useful for regulating gene expression of heterologous polynucleotide molecules in plants. The present invention also relates to expression constructs and transgenic plants containing the heterologous polynucleotide molecules.Type: GrantFiled: June 30, 2011Date of Patent: April 8, 2014Assignee: Monsanto Technology LLCInventors: Diane M. Ruezinsky, Crystal L. Hewitt, Wei Zheng
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Patent number: 8686225Abstract: The invention is directed to blood proteins produced in monocot seeds and isolated therefrom for use in therapeutic compositions, as well as to methods of making these isolated blood proteins and to therapeutic compositions comprising them.Type: GrantFiled: April 17, 2012Date of Patent: April 1, 2014Assignee: Ventria Bioscience Inc.Inventors: Ning Huang, Raymond L. Rodriguez, Frank E. Hagie, David M. Stalker
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Patent number: 8674178Abstract: Described herein are methods useful for producing proteins, such as enzymes, by agrofiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a cellulase, infecting plant cells with the Agrobacterium, allowing expression of the cellulase, and recovering the cellulase from the plant cells. In one embodiment, the protein produced is an endoglucanase.Type: GrantFiled: August 19, 2009Date of Patent: March 18, 2014Assignee: The Regents of the University of CaliforniaInventors: Karen A. McDonald, Benjamin E. Lindenmuth, Abhaya M. Dandekar, Bryce W. Falk