Involving Electroporation Patents (Class 800/292)
  • Patent number: 9756791
    Abstract: A grafting technique effective for crops such as soybean and plants of the Poaceae family including rice, wheat, corn, etc. and a method of grafting crops of the present invention comprising the steps of replacing a radicle of a dry dormant seed with a radicle of another dormant seed capable of achieving grafting, and thereafter, germinating the seed.
    Type: Grant
    Filed: November 7, 2013
    Date of Patent: September 12, 2017
    Assignee: HIROSAKI UNIVERSITY
    Inventor: Takeo Harada
  • Patent number: 9192181
    Abstract: A method for modifying a quality of a fruit includes cutting a live stem attached to a live fruit at a cut distance from the fruit, producing a cut end of the stem, contacting only the cut end of the stem with a solution comprising at least one mobile and quality-modifying food ingredient while protecting the fruit from contact with the solution, and keeping the cut end of the stem in contact with the solution for an incubation time sufficient to allow absorption and transport of the food ingredient into the fruit, the food ingredient conferring a modified quality upon the fruit. A system for practicing the method and an edible raw plant product produced by the method are also disclosed.
    Type: Grant
    Filed: April 7, 2014
    Date of Patent: November 24, 2015
    Assignee: NIGHTSHADE, LLC
    Inventors: Caius D Rommens, Heather M Holloway, Roy D Holloway
  • Patent number: 8759615
    Abstract: Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.
    Type: Grant
    Filed: February 13, 2012
    Date of Patent: June 24, 2014
    Assignee: Aurora Algae, Inc.
    Inventors: Bertrand Vick, Oliver Kilian
  • Publication number: 20140173780
    Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.
    Type: Application
    Filed: December 18, 2013
    Publication date: June 19, 2014
    Inventors: Dayakar Pareddy, Sivarama R. Chennareddy, Tatyana Minnicks, Olga Karpova, David Griffin, Jayakumar P. Samuel, Kelley A. Smith, Rodrigo Sarria-Millan, Tejinder Kumar Mall
  • Patent number: 8674176
    Abstract: The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.
    Type: Grant
    Filed: January 13, 2010
    Date of Patent: March 18, 2014
    Inventors: Ingo H. Heilmann, John Shanklin
  • Publication number: 20140020135
    Abstract: The present invention provides methods of increasing nitrogen utilization efficiency (NUE) in a transgenic plant comprising the introduction of a nucleic acid encoding a dep1 polypeptide into a plant to produce a transgenic plant that expresses the nucleic acid to produce the dep1 polypeptide, thereby resulting in an increased NUE as compared with a control plant. Also provided are methods of increasing NUE in a plant comprising reducing the amount and/or activity of a DEP1 polypeptide.
    Type: Application
    Filed: January 27, 2012
    Publication date: January 16, 2014
    Applicant: Syngenta Participations AG
    Inventors: Xiangdong Fu, Kun Wu, Qian Qian, Chengwei Zhang, Xueying Liu, Shuansuo Wang
  • Patent number: 8614366
    Abstract: In various embodiments, methods described herein comprise the use of water-soluble cationic fullerene derivatives for improving plant genetic transformation. Cationic Fullerene derivatives of the invention possess DNA binding and compaction activity and provide a new method to deliver DNA into plant cells for plant transformation. Water-soluble fullerene derivatives of the invention with anionic or non-polar substituents possess antioxidant (free radical scavenging) activity, provide improved yields and efficiency of plant transformation methods such as biolistic, Agrobacterium tumefaciens, or electroporation methods by limiting cellular damage and resulting cell death leading to higher yields of viable transformed cells in the process.
    Type: Grant
    Filed: September 7, 2010
    Date of Patent: December 24, 2013
    Inventor: Stephen R. Wilson
  • Patent number: 8604276
    Abstract: The subject application provides polynucleotides, compositions thereof and methods for regulating gene expression in a plant. Polynucleotides disclosed herein comprise novel sequences for a promoter isolated from Panicum virgatum (switchgrass) that initiates transcription of an operably linked nucleotide sequence. Thus, various embodiments of the invention comprise the nucleotide sequence of SEQ ID NO: 2 or fragments thereof comprising nucleotides 1 to 692 of SEQ ID NO: 2 that are capable of driving the expression of an operably linked nucleic acid sequence.
    Type: Grant
    Filed: June 9, 2010
    Date of Patent: December 10, 2013
    Assignee: University of Tennessee Research Foundation
    Inventors: C. Neal Stewart, David George James Mann
  • Patent number: 8581035
    Abstract: The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.
    Type: Grant
    Filed: August 31, 2007
    Date of Patent: November 12, 2013
    Assignee: Monsanto Technology LLC
    Inventor: Jyoti R. Rout
  • Patent number: 8288610
    Abstract: Methods for amplification of nucleic acids in cells are provided. Also provided are cells that contain the nucleic acids.
    Type: Grant
    Filed: February 14, 2006
    Date of Patent: October 16, 2012
    Assignees: The Biological Research Center of the Hungarian Academy of Sciences, Glaxo Group Limited
    Inventors: Gyula Hadlaczky, Aladar A. Szalay
  • Patent number: 8207399
    Abstract: The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: June 26, 2012
    Assignee: Arborgen Inc.
    Inventors: Vincent L. Chiang, Daniel T. Carraway, Richard H. Smeltzer
  • Patent number: 8193410
    Abstract: A process of producing a transgenic multi-cellular plants or parts thereof expressing a trait of interest, said trait having a controlled distribution of said trait to progeny, wherein said process comprises (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plant or cells thereof to generate progeny exhibiting said functional trait of interest due to binding between a protein or polypeptide encoded by said first heterologous nucleotide sequence and a protein or polypeptide encoded by said second heterologous nucleotide
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: June 5, 2012
    Assignee: Icon Genetics GmbH
    Inventors: Stefan Werner, Anatoly Giritch, Serik Eliby, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
  • Patent number: 8058042
    Abstract: Disclosed is a circuit arrangement comprising at least one storage device for electrical charges to generate at least one voltage pulse by selectively discharging the storage device, and at least one control unit for controlling the discharge. A controller for monitoring the chronological progression of the voltage pulse is provided which controls at least one continuation of discharge after termination. Biomaterial is treated by using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued by an additional voltage pulse.
    Type: Grant
    Filed: April 23, 2010
    Date of Patent: November 15, 2011
    Assignee: Lonza Cologne GmbH
    Inventor: Herbert Mueller-Hartmann
  • Publication number: 20110185453
    Abstract: The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm.
    Type: Application
    Filed: June 24, 2010
    Publication date: July 28, 2011
    Inventors: Vincent L. Chiang, Daniel T. Carraway, Richard H. Smeltzer
  • Patent number: 7973215
    Abstract: The invention develops a simple and reliable mushroom transformation procedure on the basis of electroporation of spores or mycelial fragments of mushroom.
    Type: Grant
    Filed: April 11, 2008
    Date of Patent: July 5, 2011
    Assignee: Mycomagic Biotechnology Co., Ltd.
    Inventor: Chun-Yi Kuo
  • Publication number: 20110162112
    Abstract: The methods provided relate to efficient methods for transforming isolated, immature maize embryos and for producing transgenic maize plantlets. The time required for the production of the transgenic plantlets and subsequent plants is significantly decreased compared to conventional methods. The methods also relate to decreasing the selection time of transgenic events and regenerating a transgenic maize plantlet from transgenic somatic embryos from the events in a plant cell culture vessel that allows for root formation and plantlet elongation in the same plant cell culture vessel.
    Type: Application
    Filed: December 22, 2010
    Publication date: June 30, 2011
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Yinghong Li, Igor C. Oliveira
  • Publication number: 20110162111
    Abstract: Methods of transforming a plant cell with a nucleic acid of interest and regenerating plants therefrom are disclosed. The methods are particularly useful for the transformation of dicot or monocot mature somatic embryos.
    Type: Application
    Filed: December 22, 2010
    Publication date: June 30, 2011
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventor: Theodore M. Klein
  • Publication number: 20110138498
    Abstract: The invention provides the disclosure of a novel plant growth pathway involving several receptor like kinases. The pathway was shown to work largely independent of brassinosteroids and involves several members of the CrRLK1L family of receptor kinases. According to the invention, HERK1, HERK2, FER, THE1 and the brassinosteroid BES1 may be modulated to influence plant growth and elongation. The invention includes methods, and transformed plants, cells tissues and seeds with increased cellular elongation and other related yield traits.
    Type: Application
    Filed: December 7, 2010
    Publication date: June 9, 2011
    Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: Hongqing Guo, Yanhai Yin
  • Publication number: 20110035845
    Abstract: The invention provides a promoter that has high expression strength and can be over-expressed in various tissues of plant, said promoter is a promoter for banana polyubiquitin (polyubiquitin) gene MhUBQ1, and has a sequence as SEQ ID No: 3. The invention provides further a gene expression cassette that contains a promoter comprising a DNA sequence as SEQ ID No: 3, and a polynucleotide having a open reading frame (ORF) linked to the 3? terminal of said promoter, wherein said promoter can activate the transcription of said polynucleotide in a organism containing said gene expression cassette. The invention provides further a gene expression vector that contains a promoter having a DNA sequence as SEQ ID No: 3. Also, the invention provides a process for producing a transgenic plant or part of organ, tissue or cell thereof containing the above-described gene expression cassette.
    Type: Application
    Filed: October 24, 2008
    Publication date: February 10, 2011
    Applicant: NATIONAL TAIWAN UNIVERSITY
    Inventors: Pung-Ling Huang, Yi-Yin Do, Wei-Fen Huang, Wei-Hsiang Lee, Yung-Chen Lin
  • Publication number: 20100293671
    Abstract: The invention relates to a composition and method for prolonging the shelf life of banana by using interfering RNA. Said method transfers a control cassette for Musa spp. ACC oxidase into banana by a novel gene transfer method, wherein said composition comprises an interfering RNA, a gene transfer expression vector and pharmaceutically acceptable carrier. Said interfering RNA can inhibit/knock-down the mRNA expression of Musa spp. ACC oxidase and inhibit the biosynthesis of ethylene in banana, thereby delaying the ripening of banana, and consequently, prolonging the shelf life of banana.
    Type: Application
    Filed: May 13, 2009
    Publication date: November 18, 2010
    Applicant: NATIONAL TAIWAN UNIVERSITY
    Inventors: Pung-Ling Huang, Yi-Yin Do, Yu-Chen Liao, Yi-Yu Lin, Shan-Shin Lee, Wei-Fen Huang
  • Publication number: 20100281580
    Abstract: Plant expression vectors that include promoter sequences operably linked to heterologous ATHK1 polynucleotides, or complements thereof, encoding polypeptides at least 95% identical to SEQ ID NO:26, where the polynucleotides encode polypeptides that confers drought resistance in the plants. Also provided are transgenic plants with increased drought resistance, methods for creating such plants, overexpressors, and underexpressors of ATHK1. Methods for enhancing drought resistance in plants are also provided.
    Type: Application
    Filed: November 5, 2009
    Publication date: November 4, 2010
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Michael R. Sussman, Dana J. Wohlbach
  • Publication number: 20100218277
    Abstract: The present invention relates to a method for the transformation of plastid genomes of plant species, in particular Asteraceae plant species, comprising the steps of providing a transformation vector carrying a DNA sequence of interest; subjecting a plant material, which comprises plastids, to a transformation treatment in order to allow the plastids to receive the transformation vector; placing the thus treated plant material for a period of time into contact with a culture medium without selection agent; subsequently placing the plant material into contact with a culture medium comprising a selection agent; and refreshing the culture medium comprising a selection agent to allow plant material comprising plastids that have acquired the DNA of interest to grow into transformants.
    Type: Application
    Filed: December 4, 2009
    Publication date: August 26, 2010
    Inventors: Cecilia Lucia Clara LELIVELT, Jackie M. Nugent, Matthew S. McCabe
  • Patent number: 7732175
    Abstract: The invention relates to a method for treating biomaterial using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued by an additional voltage pulse. The invention also relates to a circuit arrangement comprising at least one storage device for electrical charges to generate at least one voltage pulse by selectively discharging the storage device, and at least one control unit for controlling the discharge. The present invention provides a controller for monitoring the chronological progression of the voltage pulse. This controller controls at least one continuation of discharge after termination.
    Type: Grant
    Filed: June 14, 2005
    Date of Patent: June 8, 2010
    Assignee: Lonza Cologne AG
    Inventor: Herbert Mueller-Hartmann
  • Publication number: 20100071095
    Abstract: The present invention provides a method for the generation of genetically diverse plants via the incorporation of micro-satellite (MS) sequences into the planar genome. The method involves obtaining MS-like DNA fragment, introducing said DNA into plant cells, and selecting said cells for the incorporation of the DNA. Finally, plants are grown from the selected cells. Said plants display phenotypic variability, and are more diverse than the parental plant. Using the method herein presented, novel plant varieties are generated.
    Type: Application
    Filed: September 11, 2009
    Publication date: March 18, 2010
    Inventors: Iris Bahir, Shmuel Ben-Sasson
  • Patent number: 7663026
    Abstract: This invention relates to marine algae, and more particularly, to a method for producing improved seaweed strains by genetic engineering. The vector for transformation were constructed by inserting the high-plant or algae-derived promoters upstream of foreign reporter genes or such cassettes that functional genes are fused with antibiotics or herbicide-resistant genes. The genetic seaweed was generated by natural development process by recombinated plasmid DNA introduction to seaweed spore with Biolostics as transformation methods. Introduced traits of antibiotics or herbicide-resistance were used to select the transgenic seaweed individuals when foreign functional genes are transformed. Stable transformation could be obtained following this invention.
    Type: Grant
    Filed: July 7, 2003
    Date of Patent: February 16, 2010
    Assignee: Institute of Oceanology Chinese Academy of Sciences
    Inventors: Song Qin, Peng Jiang, Daozhan Yu, Fuchao Li, Guoqiong Sun
  • Patent number: 7642404
    Abstract: A process of producing a transgenic multi-cellular plants or parts thereof expressing a trait of interest, said trait having a controlled distribution of said trait to progeny, wherein said process comprises (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plant or cells thereof to generate progeny exhibiting said functional trait of interest due to binding between a protein or polypeptide encoded by said first heterologous nucleotide sequence and a protein or polypeptide encoded by said second heterologous nucleotide
    Type: Grant
    Filed: March 21, 2003
    Date of Patent: January 5, 2010
    Assignee: ICON Genetics GmbH
    Inventors: Stefan Werner, Anatoly Giritch, Serik Eliby, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
  • Publication number: 20090307802
    Abstract: The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. Additionally the present invention relates to mutant E. coli cells that contain mutated EPSPS genes.
    Type: Application
    Filed: January 10, 2007
    Publication date: December 10, 2009
    Inventors: Greg F.W. Gocal, Mark E. Knuth, Peter R. Beetham
  • Publication number: 20090288229
    Abstract: A flower tissue-specific promoter, and uses thereof, is the promoter for Phalaenopsis 1-aminocyclopropane-1-carboxylic acid synthase, ACC synthase gene PtACS2, and has a sequence as shown in SEQ ID No: 2. The invention further provides a gene expression cassette, which is composed of a promoter having a DNA sequence as SEQ ID No: 2, and a polynucleotide with an open reading frame linked to the 3? end of said promoter, wherein said promoter can activate transcription of said polynucleotide in an organism containing said gene expression cassette. The invention provides furthermore a gene expression vector, which is composed of a promoter having a DNA sequence as SEQ ID No: 2. The invention provides further a method for producing a transgenic plant or parts of organ, tissue or cell of the transgenic plant that contain a gene expression cassette described above.
    Type: Application
    Filed: May 15, 2008
    Publication date: November 19, 2009
    Applicant: Agricultrure and food Agency, Council of Agriculture, Executive Yuan
    Inventors: Pin-Hui Sung, Pung-Ling Huang, Yi-Yin Do
  • Publication number: 20090288218
    Abstract: The present invention discloses transgenic plants having an altered level of NAP protein compared to that of a non-transgenic plant, where the transgenic plants display an altered leaf senescence phenotype relative to a non-transgenic plant, as well as mutant plants comprising an inactivated NAP gene, where mutant plants display a delayed leaf senescence phenotype compared to that of a non-mutant plant. The present invention also discloses methods for delaying leaf senescence in a plant, as well as methods of making a mutant plant having a decreased level of NAP protein compared to that of a non-mutant plant, where the mutant plant displays a delayed leaf senescence phenotype relative to a non-mutant plant. Methods for causing precocious leaf senescence or promoting leaf senescence in a plant are also disclosed. Also disclosed are methods of identifying a candidate plant suitable for breeding that displays a delayed leaf senescence and/or enhanced yield phenotype.
    Type: Application
    Filed: March 28, 2007
    Publication date: November 19, 2009
    Applicant: Cornell Research Foundation, Inc.
    Inventors: Susheng Gan, Yongfeng Guo
  • Publication number: 20090235395
    Abstract: The invention concerns the use of duplex oligonucleotides about 25 to 30 base pairs to introduce site specific genetic alterations in plant cells. The oligonucleotides can be delivered by mechanical (biolistic) systems or by electroporation of plant protoplasts. Thereafter plants having the genetic alteration can be generated form the altered cells. In specific embodiments the invention concerns the alteration in the gene that encode acid invertase, UDP-glucose pyrophosphorylase, polyphenol oxidase, O-methyl transferase, cinnamyl alcohol dehydrogenase, ACC synthase and ACC oxidase or etr-1 or homolog of etr-1, and plants having isolated point mutations in such genes.
    Type: Application
    Filed: April 23, 2009
    Publication date: September 17, 2009
    Inventors: Charles J. Arntzen, Peter B. Kipp, Ramesh Kumar, Gregory D. May
  • Publication number: 20090205079
    Abstract: The present invention provides methods for increasing oil content in Jatropha Curcas L. plants. The invention further provides the cDNA and protein sequences of Jatropha acetyl CoA carboxylase (ACCase) and methods for cloning and expressing the Jatropha ACCase gene to produce transgenic plants with increased oil content.
    Type: Application
    Filed: November 10, 2008
    Publication date: August 13, 2009
    Applicant: RELIANCE LIFE SCIENCES PVT. LTD.
    Inventors: Raja Krishna Kumar, Jain Devendra, Johnson Tangirala Sudhakar
  • Patent number: 7554007
    Abstract: Provided are methods of increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass and/or increasing the yield of a plant by expressing within the plant an exogenous polynucleotide homologous to SEQ ID NO:13.
    Type: Grant
    Filed: November 22, 2005
    Date of Patent: June 30, 2009
    Assignee: Evogene Ltd.
    Inventors: Gil Ronen, Ezekiel Golan, Hagai Karchi, Rafael Meissner
  • Publication number: 20090038023
    Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.
    Type: Application
    Filed: March 10, 2006
    Publication date: February 5, 2009
    Applicant: Verenium Corporation
    Inventors: David P. Weiner, Alexander Varvak, Toby Richardson, Mircea Podar, Ellen Burke, Shaun Healey
  • Publication number: 20090025104
    Abstract: The present invention provides a method of producing a transgenic hybrid plant having no or reduced sexual reproduction, the method comprising: (a) stably transforming a first plant with a first nucleic acid construct comprising: (i) a promoter, P1; (ii) a site specific recombinase, RS1; (iii) a promoter, P2; (iv) a selectable marker, SM1; (v) a promoter P3; (vi) at least one nucleotide sequence the expression of which results in no or reduced sexual reproduction, NRSR; and (vii) at least two target sites, TRS2, specific for a site specific recombinase, RS2 that is different from the site specific recombinase, RS1, and further wherein P1 is operably located upstream of RS1, P2 is operably located upstream of SM1, P3 is operably located upstream, of NRSR, a first TRS2 is located immediately downstream of P1 and a second TRS2 is located upstream of NRSR; (b) stably transforming a second plant with a second nucleic acid construct comprising: (i) a promoter, P4; (ii) a site specific recombinase, RS2, that is diff
    Type: Application
    Filed: July 16, 2008
    Publication date: January 22, 2009
    Inventor: Hong Luo
  • Publication number: 20080235828
    Abstract: Compositions and methods for modifying the sensitivity to abscisic acid, of seeds and vegetative tissues of plants. The compositions comprise novel nucleotide molecules configured for expressing an abscisic acid-binding protein, i.e., ABAP1, comprising an amino acid sequence set forth in SEQ ID NO: 10. The methods comprise the steps of constructing a nucleotide molecule by fusing a nucleotide coding sequence set forth in SEQ ID NO: 9 with a regulatory nucleotide sequence selected for over-expression or for under-expression of the coding sequence, stably introducing the nucleotide molecule into the genome of a recipient cell from a seed-producing plant, culturing the recipient cell into a whole recipient plant and then further culturing the recipient plant to produce mature recipient seeds, and harvesting the recipient seeds. The recipient seeds and whole plants produced therefrom have modified sensitivities to ABA thereby enabling regulation of germination, physiological function and plant productivity.
    Type: Application
    Filed: October 31, 2007
    Publication date: September 25, 2008
    Applicant: University of Manitoba
    Inventors: Robert D. HILL, Fawzi A. Razam, Ashraf El-Kereamy, Santosh Kumar
  • Publication number: 20080141390
    Abstract: The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.
    Type: Application
    Filed: October 22, 2007
    Publication date: June 12, 2008
    Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: Patrick S. SCHNABLE, Sudhansu DASH
  • Patent number: 7339091
    Abstract: In this invention, two phytase genes from two generally-regarded-as-safe microorganisms, Bacillus licheniformis and Bacillus subtilis 168, were cloned and characterized. A process for phytase enzyme over-expression and purification was also developed. The enzymes have molecular weight of about 48 kilodaltons and showed extracellular phytate-hydrolyzing activities. The recombinant enzyme can be used to enhance phytase utilization in various commercial areas, including preparation of animal feed and transgenic plants that have increased growth rates for maturity, flowering and fruiting.
    Type: Grant
    Filed: November 19, 2002
    Date of Patent: March 4, 2008
    Assignee: The University of Hong Kong
    Inventors: Boon Leong Lim, Wing Kin Yip
  • Patent number: 7279624
    Abstract: According to the invention, there is provided seed and plants of the corn variety designated I015011. This invention thus relates to the plants, seeds and tissue cultures of the variety I015011, and to methods for producing a corn plant produced by crossing a corn plant of variety I015011 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I015011 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I015011, and also to the SSR and isozyme typing profiles of corn variety I015011.
    Type: Grant
    Filed: February 16, 2001
    Date of Patent: October 9, 2007
    Assignee: DeKalb Genetics Corporation
    Inventor: Thomas B. Carlson
  • Patent number: 7256325
    Abstract: Methods for the transformation of plants are provided. The methods of the present invention comprise the steps of a) contacting the meristematic tissue of a plant and an area of the plant below the contacted meristematic tissue to a power source, wherein the area below the meristematic tissue is contacted to a positive lead and the meristematic tissue is contacted with a DNA-containing medium which in turn is contacted with a negative lead, and b) applying low amperage current, thus causing the DNA to migrate from the DNA-containing medium into the cells of the meristematic tissue. The transformed plants may be grown to maturity. The mature plants may then self-pollinate and the seed from the plant harvested. Both the parent plant, seed from the parent plant, progeny plants and progeny seeds contain the introduced DNA.
    Type: Grant
    Filed: December 8, 1998
    Date of Patent: August 14, 2007
    Assignee: Board of Trustees operating Michigan State University
    Inventors: Richard F. Allison, Jeanne Ohrnberger, Rafaz Hoque, Mark Desrosiers
  • Patent number: 7220583
    Abstract: One aspect of the present invention relates to isolated nucleic acid molecules encoding avirulence proteins or polypeptides of Pseudomonas syringae pv. syringae DC 3000, or nucleic acid molecules which are complementary thereto. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The various nucleic acid molecules and proteins of the present invention can be used to impart disease resistance to a plant, make a plant hypersusceptible to colonization by nonpathogenic bacteria, modify a metabolic pathway in a cell, cause eukaryotic cell death and treat a cancerous condition, as well as inhibit programmed cell death.
    Type: Grant
    Filed: February 12, 2003
    Date of Patent: May 22, 2007
    Assignees: Cornell Research Foundation, Inc., The Board of Regents of the University of Nebraska, The United States of America, as Represented by the Secretary of Agriculture
    Inventors: James R. Alfano, Alan Collmer, Samuel W. Cartinhour, David J. Schneider
  • Patent number: 7205459
    Abstract: According to the invention, there is provided an inbred corn plant designated LIZL5. This invention thus relates to the plants, seeds and tissue cultures of the inbred corn plant LIZL5, and to methods for producing a corn plant produced by crossing the inbred corn plant LIZL5 with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing the inbred plant LIZL5 with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plant LIZL5, and also to the SSR and genetic isozyme typing profiles of inbred corn plant LIZL5. This invention further relates to methods of transforming the inbred LIZL5 and cells thereof, transformed plants produced by these methods, progeny transgenic plants, and seed derived therefrom.
    Type: Grant
    Filed: June 28, 2000
    Date of Patent: April 17, 2007
    Assignee: DeKalb Genetics Corporation
    Inventors: James R. Larkins, David D. Songstad, William L. Petersen, Hongyi Zhang, Michael T. Mann, T. Michael Spencer, Nancy G. Willetts
  • Patent number: 7205460
    Abstract: According to the invention, there is provided seed and plants of the corn variety designated I026458. This invention thus relates to the plants, seeds and tissue cultures of the variety I026458, and to methods for producing a corn plant produced by crossing a corn plant of variety I026458 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I026458 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I026458, and also to the SSR and isozyme typing profiles of corn variety I026458.
    Type: Grant
    Filed: January 29, 2001
    Date of Patent: April 17, 2007
    Assignee: DeKalb Genetics Corporation
    Inventor: Francis L. Garing
  • Patent number: 7199285
    Abstract: The present invention provides methods of modulating abscisic acid signal transduction in plants. The method comprise introducing into the plant a recombinant expression cassette comprising a promoter operably linked to an ABH1 polynucleotide.
    Type: Grant
    Filed: December 18, 2003
    Date of Patent: April 3, 2007
    Assignee: University of California, San Diego
    Inventors: Julian Schroeder, Veronique Hugouvieux, June M. Kwak
  • Patent number: 7169981
    Abstract: According to the invention, there is provided seed and plants of the corn variety designated I015036. This invention thus relates to the plants, seeds and tissue cultures of the variety I015036, and to methods for producing a corn plant produced by crossing a corn plant of variety I015036 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I015036 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I015036, and also to the SSR and isozyme typing profiles of corn variety I015036.
    Type: Grant
    Filed: January 29, 2001
    Date of Patent: January 30, 2007
    Assignee: DeKalb Genetics Corporation
    Inventor: Thomas B. Carlson
  • Patent number: 7122715
    Abstract: The present invention relates generally to transgenic plants. More specifically, it relates to methods and compositions for the introduction of DNA using circular molecules that are not able to replicate outside a host cell. The circular molecules contain site-specific recombination sequences and allow transformation of host cells with DNA comprising only selected sequences of interest.
    Type: Grant
    Filed: September 20, 2001
    Date of Patent: October 17, 2006
    Assignee: Monsanto Technology LLC
    Inventors: John A. Korte, Brenda A. Lowe
  • Patent number: 7081567
    Abstract: Disclosed is a method for making a bioreactor comprising a foreign target gene, special selectable markers and Dunaliella Salina as host. It is prepared by the genetic transformation techniques that include introducing a foreign target gene into the cells of Dunaliella Salina and screening the transformed cells of Dunaliella Salina. The bioreactor of the present invention can be used as a safe and cheap production system for proteins of pharmaceutical interest including vaccines, especially oral products, in a large scale, because the cells of Dunaliella Salina are easy of genetic manipulation in preparation of the bioreactor, nontoxic and edible for humans and animals, and harmless to the environment.
    Type: Grant
    Filed: November 29, 2001
    Date of Patent: July 25, 2006
    Assignees: Zhengzhou University
    Inventors: Lexun Xue, Weidong Pan, Guozhong Jiang, Jianmin Wang
  • Patent number: 7067716
    Abstract: The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus production of elite lines of soybean.
    Type: Grant
    Filed: July 14, 2004
    Date of Patent: June 27, 2006
    Assignee: Stine Biotechnology, Inc.
    Inventors: Bruce Marvin Held, Herbert Martin Wilson, Liming Hou, Carol Jean Lewnau, Janelle Christine Eby
  • Patent number: 7015372
    Abstract: The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited plant artificial chromosomes (PLACs) which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these regions will prove valuable in isolating additional centromeric and centromere related genetic elements and polypeptides from other species.
    Type: Grant
    Filed: August 6, 2002
    Date of Patent: March 21, 2006
    Assignee: University of Chicago
    Inventors: Daphne Preuss, Gregory Copenhaver
  • Patent number: 6972197
    Abstract: The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited minichromosomes which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these regions will prove valuable in isolating additional centromeric and centromere related genetic elements and polypeptides from other species.
    Type: Grant
    Filed: March 17, 2000
    Date of Patent: December 6, 2005
    Assignee: The University of Chicago
    Inventors: Daphne Preuss, Gregory Copenhaver, Kevin C. Keith
  • Patent number: 6911586
    Abstract: A novel garden bean cultivar, designated ‘210104’, is disclosed. The invention relates to the seeds of garden bean cultivar ‘210104’, to the plants of garden bean line ‘210104’ and to methods for producing a bean plant by crossing the cultivar ‘210104’ with itself or another bean line. The invention further relates to methods for producing a bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar ‘210104’.
    Type: Grant
    Filed: February 25, 2002
    Date of Patent: June 28, 2005
    Assignee: Harris Moran Seed Company
    Inventor: Robert Gehin