Involving Particle-mediated Transfecion (e.g., Biolistic, Etc.) Patents (Class 800/293)
  • Patent number: 11198882
    Abstract: As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.
    Type: Grant
    Filed: August 24, 2018
    Date of Patent: December 14, 2021
    Inventors: Lakshmi Sastry-Dent, Zehui Cao, Shreedharan Sriram, Steven R. Webb, Debra L. Camper
  • Patent number: 11098316
    Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: August 24, 2021
    Assignee: Corteva Agriscience LLC
    Inventors: Lakshmi Sastry-Dent, Zehui Cao, Shreedharan Sriram, Steven R. Webb, Debra L. Camper, W. Michael Ainley
  • Patent number: 10253322
    Abstract: Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
    Type: Grant
    Filed: May 6, 2016
    Date of Patent: April 9, 2019
    Assignee: MONSANTO TECHNOLOGY LLC
    Inventors: Yinghui Dan, Joyce E. Fry
  • Patent number: 9353377
    Abstract: Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
    Type: Grant
    Filed: May 24, 2012
    Date of Patent: May 31, 2016
    Assignee: Monsanto Technology LLC
    Inventors: Yinghui Dan, Joyce E. Fry
  • Publication number: 20150128305
    Abstract: The present invention provides herein a polynucleotide sequence encoding the tubby-like protein, CaTLP1, from chickpea (Cicer arientium L.) that is responsive to abiotic stress and is involved in plant growth and development. Further, the recombinant DNA construct and recombinant vector comprising the polynucleotide sequence encoding CaTLP1, host cell comprising the recombinant vector and a process for producing a transgenic plant that expresses the CaTLP1 protein are also provided herein.
    Type: Application
    Filed: May 7, 2013
    Publication date: May 7, 2015
    Inventors: Niranjan Chakraborty, Subhra Chakraborty, Asis Datta, Vijay Wardhan, Kishwer Jahan
  • Patent number: 9024112
    Abstract: A novel means for accelerating the flowering time in Rosaceae fruit trees such as apples and pears is provided, by inoculating a viral RNA into a cotyledon of a Rosaceae fruit tree seedling immediately after rooting with a particle gun technique, wherein the viral RNA is concentrated from a proliferating host infected with a recombinant apple latent spherical virus (FT-ALSV) expressing an Arabidopsis FT gene.
    Type: Grant
    Filed: February 12, 2010
    Date of Patent: May 5, 2015
    Assignee: Incorporated National University Iwate University
    Inventors: Nobuyuki Yoshikawa, Noriko Yamagishi, Shintaro Sasaki, Sadao Komori, Masato Wada, Norimitsu Tanaka
  • Patent number: 8993845
    Abstract: The invention relates to the genetic manipulation of plants, particularly plants of the genus Brassica. Methods are provided for producing transgenic Brassica plants involving the introduction of a DNA construct by microprojectile bombardment into pre-incubated microspores, microspore-derived embryos and microspore-derived hypocotyls. The methods find use in the development of improved agricultural varieties of Brassica plants through the incorporation of desirable agronomic traits.
    Type: Grant
    Filed: July 1, 2014
    Date of Patent: March 31, 2015
    Assignee: Pioneer Hi-Bred International Inc
    Inventors: Wenpin Chen, Lomas Tulsieram
  • Patent number: 8901372
    Abstract: This invention relates to a nucleic acid construct. The construct includes a nucleic acid molecule configured to silence Banana bunchy top virus (BBTV), a 5? DNA promoter sequence, and a 3? terminator sequence. The nucleic acid molecule, the promoter, and the terminator are operatively coupled to permit transcription of the nucleic acid molecule. The present invention also relates to expression vectors, host cells, and transgenic plants containing the nucleic acid construct of the present invention. Also disclosed are methods of imparting BBTV resistance to plants.
    Type: Grant
    Filed: February 25, 2010
    Date of Patent: December 2, 2014
    Assignee: University of Hawaii
    Inventors: John Hu, Wayne Borth, Eden A. Perez, Kheng Cheah
  • Patent number: 8884099
    Abstract: The present invention relates to a method for the transformation of plastid genomes of plant species, in particular Asteraceae plant species, comprising the steps of providing a transformation vector carrying a DNA sequence of interest; subjecting a plant material, which comprises plastids, to a transformation treatment in order to allow the plastids to receive the transformation vector; placing the thus treated plant material for a period of time into contact with a culture medium without selection agent; subsequently placing the plant material into contact with a culture medium comprising a selection agent; and refreshing the culture medium comprising a selection agent to allow plant material comprising plastids that have acquired the DNA of interest to grow into transformants.
    Type: Grant
    Filed: December 4, 2009
    Date of Patent: November 11, 2014
    Assignee: Rijk Zwaan Zaadteelt en Zaadhandel B.V.
    Inventors: Cecilia Lucia Clara Lelivelt, Jackie M. Nugent, Matthew S. McCabe
  • Publication number: 20140317784
    Abstract: The invention relates to the genetic manipulation of plants, particularly plants of the genus Brassica. Methods are provided for producing transgenic Brassica plants involving the introduction of a DNA construct by microprojectile bombardment into pre-incubated microspores, microspore-derived embryos and microspore-derived hypocotyls. The methods find use in the development of improved agricultural varieties of Brassica plants through the incorporation of desirable agronomic traits.
    Type: Application
    Filed: July 1, 2014
    Publication date: October 23, 2014
    Inventors: WENPIN CHEN, LOMAS TULSIERAM
  • Patent number: 8865969
    Abstract: According to the present invention, a technique of increasing the frequency of genetic recombination in genomic DNA of a plant is established. Such technique comprises: introducing a restriction enzyme gene that can be expressed in a target plant cell into the plant cell and causing the restriction enzyme gene to be transiently expressed so as to induce genetic recombination of genomic DNA; or introducing a promoter and a restriction enzyme gene using the Agrobacterium method so as to induce genetic recombination of genomic DNA.
    Type: Grant
    Filed: January 18, 2011
    Date of Patent: October 21, 2014
    Assignees: Toyota Jidosha Kabushiki Kaisha, Riken
    Inventors: Satoshi Kondo, Chikara Ohto, Kunihiro Ohta, Shuichi Ohsato, Norihiro Mitsukawa, Nobuhiko Muramoto, Hiroki Sugimoto
  • Patent number: 8853488
    Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: October 7, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Jyoti R. Rout, Brenda A. Lowe, John Purcell, Anne Spelletich, Michael Spencer, Melissa Way
  • Patent number: 8796508
    Abstract: The invention relates to the genetic manipulation of plants, particularly plants of the genus Brassica. Methods are provided for producing transgenic Brassica plants involving the introduction of a DNA construct by microprojectile bombardment into pre-incubated microspores, microspore-derived embryos and microspore-derived hypocotyls. The methods find use in the development of improved agricultural varieties of Brassica plants through the incorporation of desirable agronomic traits.
    Type: Grant
    Filed: November 10, 2005
    Date of Patent: August 5, 2014
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Wenpin Chen, Lomas Tulsieram
  • Publication number: 20140208462
    Abstract: The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ECS) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ECS (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (ExNA) operably linked to the ECS; (ii) an expression vector may comprise an ECS; an ExNA; and, optionally, a 3? termination sequence, wherein the ECS has promoter activity sufficient to express the ExNA in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an ExNA in a plant (e.g.
    Type: Application
    Filed: February 20, 2014
    Publication date: July 24, 2014
    Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
    Inventors: Mona DAMAJ, T. Erik MIRKOV
  • Publication number: 20140173780
    Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.
    Type: Application
    Filed: December 18, 2013
    Publication date: June 19, 2014
    Inventors: Dayakar Pareddy, Sivarama R. Chennareddy, Tatyana Minnicks, Olga Karpova, David Griffin, Jayakumar P. Samuel, Kelley A. Smith, Rodrigo Sarria-Millan, Tejinder Kumar Mall
  • Patent number: 8742202
    Abstract: Methods for the transformation of sugar cane are provided. The methods comprise utilizing sugar cane immature shoots as the source of plant material for transformation. Segments of the immature shoot are excised and transformed by any suitable transformation methodology. In some embodiments, the segments are cultured in embryogenic culture induction medium prior to transformation. Transformation can be performed via Agrobacterium-mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: June 3, 2014
    Assignee: Syngenta Participations AG
    Inventor: Heng Zhong
  • Patent number: 8722410
    Abstract: Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.
    Type: Grant
    Filed: October 3, 2008
    Date of Patent: May 13, 2014
    Assignee: Dow AgroSciences, LLC.
    Inventors: Jayakumar P. Samuel, Frank Burroughs, Suraj Dixit, Mark W. Zettler
  • Patent number: 8710207
    Abstract: The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ECS) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ECS (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (ExNA) operably linked to the ECS; (ii) an expression vector may comprise an ECS; an ExNA; and, optionally, a 3? termination sequence, wherein the ECS has promoter activity sufficient to express the ExNA in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an ExNA in a plant (e.g.
    Type: Grant
    Filed: May 10, 2011
    Date of Patent: April 29, 2014
    Assignee: The Texas A&M University Systems
    Inventors: T. Erik Mirkov, Jong Won Park, San-Ji Gao
  • Publication number: 20140096284
    Abstract: The invention provides particles and methods to deliver freeze- or air-dried molecules to cells.
    Type: Application
    Filed: March 5, 2013
    Publication date: April 3, 2014
    Inventors: Susana Martin-Ortigosa, Kan Wang
  • Patent number: 8686222
    Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using dendrimers, and optionally one or more Cell Penetrating Peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.
    Type: Grant
    Filed: October 8, 2010
    Date of Patent: April 1, 2014
    Assignee: Dow AgroSciences, LLC.
    Inventors: Jayakumar P. Samuel, Narasimha C. Samboju, Kerrm Y. Yau, Steven R. Webb, Frank Burroughs
  • Patent number: 8680366
    Abstract: The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.
    Type: Grant
    Filed: June 9, 2008
    Date of Patent: March 25, 2014
    Assignee: Agriculture and Agri-Food Canada
    Inventors: Francois Eudes, Archana Chugh
  • Patent number: 8674176
    Abstract: The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.
    Type: Grant
    Filed: January 13, 2010
    Date of Patent: March 18, 2014
    Inventors: Ingo H. Heilmann, John Shanklin
  • Patent number: 8653327
    Abstract: Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.
    Type: Grant
    Filed: July 7, 2011
    Date of Patent: February 18, 2014
    Assignee: Agrigenetics, Inc.
    Inventors: Narasimha C. Samboju, Kerrm Y. Yau, Frank Burroughs, Jayakumar P. Samuel, Steven R. Webb
  • Publication number: 20140020135
    Abstract: The present invention provides methods of increasing nitrogen utilization efficiency (NUE) in a transgenic plant comprising the introduction of a nucleic acid encoding a dep1 polypeptide into a plant to produce a transgenic plant that expresses the nucleic acid to produce the dep1 polypeptide, thereby resulting in an increased NUE as compared with a control plant. Also provided are methods of increasing NUE in a plant comprising reducing the amount and/or activity of a DEP1 polypeptide.
    Type: Application
    Filed: January 27, 2012
    Publication date: January 16, 2014
    Applicant: Syngenta Participations AG
    Inventors: Xiangdong Fu, Kun Wu, Qian Qian, Chengwei Zhang, Xueying Liu, Shuansuo Wang
  • Publication number: 20140007299
    Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
    Type: Application
    Filed: May 21, 2013
    Publication date: January 2, 2014
    Inventors: Brian Martinell, Michael W. Petersen, David A. Somers, Yuechun Wan, Edward Williams, Xudong Ye
  • Patent number: 8614366
    Abstract: In various embodiments, methods described herein comprise the use of water-soluble cationic fullerene derivatives for improving plant genetic transformation. Cationic Fullerene derivatives of the invention possess DNA binding and compaction activity and provide a new method to deliver DNA into plant cells for plant transformation. Water-soluble fullerene derivatives of the invention with anionic or non-polar substituents possess antioxidant (free radical scavenging) activity, provide improved yields and efficiency of plant transformation methods such as biolistic, Agrobacterium tumefaciens, or electroporation methods by limiting cellular damage and resulting cell death leading to higher yields of viable transformed cells in the process.
    Type: Grant
    Filed: September 7, 2010
    Date of Patent: December 24, 2013
    Inventor: Stephen R. Wilson
  • Patent number: 8609420
    Abstract: Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: December 17, 2013
    Assignee: Dow AgroSciences, LLC.
    Inventors: Jayakumar P. Samuel, Narasimha C. Samboju, Kerrm Y. Yau, Gaofeng Lin, Steven R. Webb, Frank Burroughs
  • Patent number: 8581035
    Abstract: The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.
    Type: Grant
    Filed: August 31, 2007
    Date of Patent: November 12, 2013
    Assignee: Monsanto Technology LLC
    Inventor: Jyoti R. Rout
  • Patent number: 8581036
    Abstract: A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.
    Type: Grant
    Filed: March 8, 2011
    Date of Patent: November 12, 2013
    Assignee: Dow AgroSciences, LLC.
    Inventors: Narasimha C. Samboju, Jayakumar P. Samuel, Gaofeng Lin, Steven R. Webb, Frank Burroughs
  • Patent number: 8575424
    Abstract: Methods for introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall include use of nanoparticles. In some embodiments, the cell comprising a cell wall is a cultured plant cell. Methods include genetically or otherwise modifying plant cells and for treating or preventing disease in any plant, especially crop plants. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with functionalized linear nucleic acid cassette molecules.
    Type: Grant
    Filed: July 7, 2011
    Date of Patent: November 5, 2013
    Assignee: Dow AgroSciences, LLC.
    Inventors: Kerrm Y. Yau, Jayakumar P. Samuel, Frank Burroughs, Narasimha C. Samboju, Steven R. Webb
  • Patent number: 8461417
    Abstract: Reducing /yso-phosphatidylcholine acyltransferase (LPCAT) activity in a plant increases seed oil content and/or fatty acid levels in the plant. Increases in the level of unusual fatty acids 16:3, 18:3 and 20:1 c11 are particularly pronounced.
    Type: Grant
    Filed: May 21, 2009
    Date of Patent: June 11, 2013
    Assignee: National Research Council of Canada
    Inventors: Jitao Zou, Qilin Chen, Wenyun Shen, Liping Wang
  • Publication number: 20130145488
    Abstract: The invention provides gold-plated mesoporous silicate bodies comprising pores and at least one agent and methods of using those bodies.
    Type: Application
    Filed: December 6, 2012
    Publication date: June 6, 2013
    Applicant: Iowa State University Research Foundation, Inc.
    Inventors: Kan Wang, Brian G. Trewyn, Susana Martin-Ortigosa, Justin Valenstein
  • Patent number: 8420891
    Abstract: Disclosed is a method for introducing a foreign substance (3) into a cell (1) having a cell wall. The method comprises the steps of: providing a carbon nanotube (2) carrying at least one enzyme capable of decomposing a cell wall; supplying the carbon nanotube (2) and the foreign substance (3) into a treatment solution containing the cell (1); decomposing the cell wall of the cell (1) by the action of the enzyme carried on the carbon nanotube (2) upon the contact of the carbon nanotube (2) with the cell (1); and introducing the foreign substance (3) into the cell (1) through a site decomposed with the enzyme on the cell wall.
    Type: Grant
    Filed: June 11, 2008
    Date of Patent: April 16, 2013
    Assignee: National University Corporation Nagoya University
    Inventors: Yoshinobu Baba, Manabu Tokeshi, Noritada Kaji, Ibrahim Maged Fouad Serag Eldin Bayoumi
  • Publication number: 20130074214
    Abstract: Isolated nucleic acids and proteins and plants expressing the same for improved nitrogen utilization, increased yield, and increased stress tolerance.
    Type: Application
    Filed: September 25, 2009
    Publication date: March 21, 2013
    Applicants: University Of Guelph, Syngenta Participations
    Inventors: Surya Kant, Steven Rothstein, Yong-Mei Bi, Joseph Clarke
  • Patent number: 8395020
    Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
    Type: Grant
    Filed: August 31, 2007
    Date of Patent: March 12, 2013
    Assignee: Monsanto Technology LLC
    Inventors: Jyoti R. Rout, Brenda A. Lowe, John Purcell, Anne Spelletich, Michael Spencer, Melissa Way
  • Publication number: 20120311734
    Abstract: The present disclosure relates, in some embodiments, to potato transformation compositions, systems, methods, microorganisms, and plants (e.g., one or more potato chipping varieties). In some embodiments, a method of transforming and/or transfecting a plant (e.g., ‘Atlantic’ potato) may comprise (a) growing an ‘Atlantic’ potato plant (e.g., from a tuber) for from about 3 weeks to about 4 weeks, (b) removing one or more leaf sections (e.g., each section from about 0.5 cm to about 1 cm in its longest dimension) from the plant, (c) cultivating the one or more sections on a callus induction medium comprising zeatin for about 2 days, and/or (d) contacting the one or more sections with Agrobacterium comprising the exogenous nucleic acids under conditions that permit transfer of the exogenous nucleic acid to the one or more sections to produce at least one transformed and/or transfected plant cell.
    Type: Application
    Filed: June 4, 2012
    Publication date: December 6, 2012
    Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
    Inventors: Ian S. Curtis, Javier Gonzalez-Ramos, T. Erik Mirkov
  • Publication number: 20120297506
    Abstract: Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
    Type: Application
    Filed: May 24, 2012
    Publication date: November 22, 2012
    Inventors: Yinghui Dan, Joyce E. Fry
  • Publication number: 20120266328
    Abstract: The invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, ?-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.
    Type: Application
    Filed: January 20, 2012
    Publication date: October 18, 2012
    Applicant: BP CORPORATION NORTH AMERICA INC.
    Inventors: KEVIN A. GRAY, LISHAN ZHAO, MICHELLE H. CAYOUETTE
  • Publication number: 20120266329
    Abstract: The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs.
    Type: Application
    Filed: February 20, 2012
    Publication date: October 18, 2012
    Applicant: BP Corporation North America Inc.
    Inventors: Eric J. Mathur, Cathy Chang
  • Patent number: 8288610
    Abstract: Methods for amplification of nucleic acids in cells are provided. Also provided are cells that contain the nucleic acids.
    Type: Grant
    Filed: February 14, 2006
    Date of Patent: October 16, 2012
    Assignees: The Biological Research Center of the Hungarian Academy of Sciences, Glaxo Group Limited
    Inventors: Gyula Hadlaczky, Aladar A. Szalay
  • Publication number: 20120219580
    Abstract: The present invention relates to a method for producing transgenic plants, which involves producing hemagglutinin proteins of the H5N1 virus using a plant transformation recombinant vector, wherein said vector can express hemagglutinin proteins of the H5N1 virus, the highly pathogenic avian influenza virus, and transport proteins expressed in plants to the endoplasmic reticulum and enable the retention of the proteins in the endoplasmic reticulum to enable glycosylation required for antigen activity. The present invention also relates to a method for the mass production of hemagglutinin proteins of the antigenic H5N1 virus from transgenic plants or a vaccine composition for the avian influenza virus comprising the transgenic plants or the hemagglutinin proteins produced from the plants. The transgenic plants can be used as edible vaccines, antigenic hemagglutinin proteins produced can be used as protein vaccines for the H5N1 avian influenza virus, or as diagnostic reagents for avian influenza virus infection.
    Type: Application
    Filed: October 6, 2010
    Publication date: August 30, 2012
    Applicant: Helix, Co., Limited
    Inventors: In Hwan Hwang, Eun Ju Sohn, Eun Hye Kwon, Yun Jeong Na, Eun Hyun Jeon, Se Jin Im, Ki Seok Park, Young Chul Sung
  • Publication number: 20120210467
    Abstract: In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins.
    Type: Application
    Filed: October 8, 2010
    Publication date: August 16, 2012
    Applicant: DSM IP ASSETS B.V.
    Inventors: Nelson R. Barton, Tim S. Hitchman, Jonathan D. Lyon, Eileen O'Donaghue, Mark A. Wall
  • Patent number: 8212109
    Abstract: Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
    Type: Grant
    Filed: June 3, 2002
    Date of Patent: July 3, 2012
    Assignee: Monsanto Technology LLC
    Inventors: Yinghui Dan, Joyce E. Fry
  • Patent number: 8207399
    Abstract: The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: June 26, 2012
    Assignee: Arborgen Inc.
    Inventors: Vincent L. Chiang, Daniel T. Carraway, Richard H. Smeltzer
  • Patent number: 8193410
    Abstract: A process of producing a transgenic multi-cellular plants or parts thereof expressing a trait of interest, said trait having a controlled distribution of said trait to progeny, wherein said process comprises (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plant or cells thereof to generate progeny exhibiting said functional trait of interest due to binding between a protein or polypeptide encoded by said first heterologous nucleotide sequence and a protein or polypeptide encoded by said second heterologous nucleotide
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: June 5, 2012
    Assignee: Icon Genetics GmbH
    Inventors: Stefan Werner, Anatoly Giritch, Serik Eliby, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
  • Publication number: 20120090053
    Abstract: A novel means for accelerating the flowering time in Rosaceae fruit trees such as apples and pears is provided, by inoculating a viral RNA into a cotyledon of a Rosaceae fruit tree seedling immediately after rooting with a particle gun technique, wherein the viral RNA is concentrated from a proliferating host infected with a recombinant apple latent spherical virus (FT-ALSV) expressing an Arabidopsis FT gene.
    Type: Application
    Filed: February 12, 2010
    Publication date: April 12, 2012
    Inventors: Nobuyuki Yoshikawa, Noriko Yamagishi, Shintaro Sasaki, Sadao Komori, Masato Wada, Norimitsu Tanaka
  • Publication number: 20120084884
    Abstract: Methods that provide for stable transformation of fern spores and/or protonemata by Agrobacterium- and/or by particle-mediated transformation are disclosed. Also provided are stably transformed, non-chimeric ferns.
    Type: Application
    Filed: October 5, 2011
    Publication date: April 5, 2012
    Applicant: University of Tennessee Research Foundation
    Inventors: Neal Stewart, Muthukumar Balasubramaniam, Blake Joyce
  • Publication number: 20120054918
    Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
    Type: Application
    Filed: August 24, 2011
    Publication date: March 1, 2012
    Inventors: Erik Dersch, Richard J. Heinzen, Brian Martinell, Anatoly Rivlin, Yuechun Wan, Xudong Ye
  • Patent number: 8119859
    Abstract: Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.
    Type: Grant
    Filed: June 8, 2009
    Date of Patent: February 21, 2012
    Assignee: Aurora Algae, Inc.
    Inventors: Bertrand Vick, Oliver Kilian
  • Publication number: 20120017334
    Abstract: The present invention relates to glutamate decarboxylase (GAD) gene sequence isolated from Oryza sativa (cv Rasi) and their corresponding encoded polypeptides that confer the traits of improved nitrogen use efficiency in plants. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having improved nitrogen use efficiency.
    Type: Application
    Filed: July 7, 2009
    Publication date: January 19, 2012
    Applicant: Avesthagen Limited
    Inventors: Villoo Morawala-Patell, Mahesh Venkataramaiah, Suhas Nimbalkar, Manjula Ramakrishna, Suresh Sedasivam