Via Agrobacterium Patents (Class 800/294)
  • Patent number: 11168319
    Abstract: Plant cell cultures as well as related methods, systems, and compositions for increasing the frequency and efficiency of plant genome editing are provided. Various plant cell growth conditions and/or treatments where such increases in gene editing frequencies are obtained are disclosed.
    Type: Grant
    Filed: February 28, 2018
    Date of Patent: November 9, 2021
    Assignee: INARI AGRICULTURE TECHNOLOGY, INC.
    Inventors: Erik William Clement, Yajie Niu, Frank Anthony Skraly
  • Patent number: 11106225
    Abstract: A UAV comprises a camera and a controller. The controller is configured to: (a) receive image data from the camera, (b) determine, based on the received image data, whether or not a predetermined visibility condition associated with an operator of the UAV is satisfied, and (c) perform a predetermined action to attempt to operate in accordance with a predetermined visibility state with respect to the operator of the UAV based on a result of the determination.
    Type: Grant
    Filed: August 27, 2018
    Date of Patent: August 31, 2021
    Inventor: Iain Matthew Russell
  • Patent number: 10995339
    Abstract: The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.
    Type: Grant
    Filed: November 22, 2016
    Date of Patent: May 4, 2021
    Inventors: Andrew Carl Crow, Scott Henry Diehn, Albert Laurence Lu, Carl Robert Simmons, Lynne Eileen Sims
  • Patent number: 10920234
    Abstract: Provided is a method that can efficiently produce a transgenic Taraxacum plant in a short period. A method of producing a transgenic Taraxacum plant, including: an infection step of infecting a tissue fragment from a Taraxacum plant with an Agrobacterium tumefaciens containing a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.
    Type: Grant
    Filed: June 7, 2019
    Date of Patent: February 16, 2021
    Assignee: SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventors: Yukino Inoue, Haruhiko Yamaguchi, Teruhiko Terakawa, Tsubasa Yano
  • Patent number: 10813305
    Abstract: Cosmos bipinnatus cotyledons are isolated as explants, and the isolated explants are cultured onto a regeneration medium to obtain regenerated Cosmos bipinnatus shoots. The regeneration medium optionally includes a basal medium, a cytokinin, an ethylene action inhibitor, and a nitrogen source.
    Type: Grant
    Filed: December 5, 2016
    Date of Patent: October 27, 2020
    Inventors: Mahdi Jaberi, Pejman Azadi
  • Patent number: 10752910
    Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: August 25, 2020
    Assignee: Monsanto Technology LLC
    Inventor: Stanislaw Flasinski
  • Patent number: 10731170
    Abstract: The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: August 4, 2020
    Assignee: Monsanto Technology LLC
    Inventor: Stanislaw Flasinski
  • Patent number: 10717983
    Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: July 21, 2020
    Assignee: Monsanto Technology LLC
    Inventors: Erik Dersch, Richard J. Heinzen, Brian J. Martinell, Anatoly Rivlin, Yuechun Wan, Xudong Ye
  • Patent number: 10689633
    Abstract: Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol. In other exemplary embodiments, methods and materials are disclosed for degrading mannans.
    Type: Grant
    Filed: October 24, 2012
    Date of Patent: June 23, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventor: Henry Daniell
  • Patent number: 10683521
    Abstract: An Expression system; isolated nucleic acid molecule or host cell comprising: (i) A first gene encoding for a first enzyme linked to a first promoter, wherein the first promoter is a time delay promoter; (ii) A second gene encoding for a second enzyme capable of using the product generated by the first enzyme as a substrate, wherein the second gene is operably linked to a second promoter, wherein the second promoter is inducible by the product generated by the first enzyme; (iii) Optionally, a third gene encoding a transcription factor that represses expression of the second gene in the absence of the product generated by the first enzyme, wherein the third gene is operably linked to a third promoter that regulates expression of the third gene; and its use in producing a product such as hydroxybenzaldehyde.
    Type: Grant
    Filed: June 12, 2013
    Date of Patent: June 16, 2020
    Assignee: Nanyang Technological University
    Inventors: Matthew Wook Chang, Tat Ming Samuel Lo, Chueh Loo Poh
  • Patent number: 10647990
    Abstract: The present invention provides a new plant gene-rice high temperature resistance 1 gene (Rice High Temperature Resistance 1, HTR1) and encoded protein thereof. Also disclosed is the use of the high temperature resistance gene, especially for the enhancement of high-temperature resistance of plants in plant variety improvement and cross breeding.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: May 12, 2020
    Assignee: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES
    Inventors: Hongxuan Lin, Xinmin Li, Jiping Gao, Junxiang Shan, Min Shi
  • Patent number: 10645888
    Abstract: The present invention provides medium compositions and methods for the regeneration of the whole plant from explants obtained from plants belonging to the Malvaceae family, particularly the Abelmoschus genus, more preferably Abelmoschus esculentus L, through somatic embryogenesis. The present invention also provides an efficient methodology for genetic transformation of plants belonging to the Malvaceae family through somatic embryogenesis in semisolid culture with the use of the Agrobacterium. The present invention is also related to a method for the development of virus-resistant transgenic plants belonging to the Malvaceae family.
    Type: Grant
    Filed: January 27, 2016
    Date of Patent: May 12, 2020
    Assignee: RASI SEEDS PRIVATE LIMITED
    Inventors: Poovannan Kandasami, Sabaripriya Ravindran, Mohanraj Perumal, Manonmani Elangovan, Packialakshmi Maruthayee Rajendran, Saravanakumar Marappan, Subramanian Vaidyanathan, Ramasami Muthugounder
  • Patent number: 10638679
    Abstract: The present invention relates to the generation and cultivation of plant cell material in the form of a non-tissue multilayer cell pack and its use for the accumulation or harvesting of a desired product. In particular, the invention provides a method for the generation of plant cell material in the form of a medium-deprived, porous structured and non-tissue multilayer cell pack and for the subsequent maintenance of said cell pack, comprising the steps of (i) providing a cell pack by separating cells from a plant cell suspension culture, wherein the content of the liquid comprised by the cell pack is reduced and adjusted to correspond to a cell pack density between 0.1 and 0.9 g wet cell weight per cm3, thereby establishing the medium-deprived and porous structured nature of said cell pack, and (ii) incubating said medium-deprived and porous structured cell pack in a non-liquid environment under a relative humidity of 50 to 100%.
    Type: Grant
    Filed: January 31, 2013
    Date of Patent: May 5, 2020
    Assignee: Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V
    Inventor: Thomas Rademacher
  • Patent number: 10167479
    Abstract: The present invention relates to the field of the production of transgenic plants through Agrobacterium-mediated transformation of cells of somatic embryogenic calli or embryogenic suspension cultures and regeneration of the transformed cells into fruit-setting plants. In particular, the present invention relates to the production of transgenic plants in the Euphorbiaceae family. The present invention further relates to media compositions, selection methods and engineered Agrobacterium tumefaciens strains that improve Agrobacterium-mediated transformation efficiency.
    Type: Grant
    Filed: December 4, 2009
    Date of Patent: January 1, 2019
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lin Cai, Longhua Sun, Lin Fu, Lianghui Ji
  • Patent number: 9885053
    Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: February 6, 2018
    Assignee: Monsanto Technology LLC
    Inventors: Erik Dersch, Richard J. Heinzen, Brian J. Martinell, Anatoly Rivlin, Yuechun Wan, Xudong Ye
  • Patent number: 9790512
    Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: October 17, 2017
    Assignee: Monsanto Technology LLC
    Inventors: Beth Jo Calabotta, Kevin Lee Deppermann, Erik D. Dersch, Jerald D. Heise, Angela Ranae Koestel, Cindy L. Ludwig, Brian J. Martinell, Edward Williams
  • Patent number: 9777047
    Abstract: Compositions and methods are described which use RAV proteins and genes that encode such proteins to regulate RNA silencing in plant cells. Novel ntRAV proteins and genes are also described.
    Type: Grant
    Filed: April 14, 2014
    Date of Patent: October 3, 2017
    Assignee: University of South Carolina
    Inventors: Vicki Sue Vance, Lewis H. Bowman, Matthew W. Endres
  • Patent number: 9232703
    Abstract: Compositions and methods related to transgenic plants comprising seed production technology are provided. Specifically, maize plants having a E6611.32.1.38 event which confers seed production technology are provided. The plant harboring the E6611.32.1.38 event at the recited chromosomal location comprises the genomic/transgene junctions described. The plant genomic DNA flanking the integrated E6611.32.1.38 event can be used to design assays that will be specific for the E6611.32.1.38 event. The characterization of the genomic insertion site of the E6611.32.1.38 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the maize E6611.32.1.38 event are provided.
    Type: Grant
    Filed: August 6, 2012
    Date of Patent: January 12, 2016
    Assignees: PIONEER HI BRED INTERNATIONAL INC, E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Kent Brink, Erin Crowgey, Nina Dietrich, David Hondred, Joshua K Young, Cathy Xiaoyan Zhong
  • Publication number: 20150143587
    Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
    Type: Application
    Filed: January 16, 2015
    Publication date: May 21, 2015
    Inventors: Anisha Akula, David R. Duncan, Brenda A. Lowe, Michael T. Mann, William L. Petersen, Jyoti R. Rout, David D. Songstad, Joel B. Wilks, Wanggen Zhang
  • Publication number: 20150128305
    Abstract: The present invention provides herein a polynucleotide sequence encoding the tubby-like protein, CaTLP1, from chickpea (Cicer arientium L.) that is responsive to abiotic stress and is involved in plant growth and development. Further, the recombinant DNA construct and recombinant vector comprising the polynucleotide sequence encoding CaTLP1, host cell comprising the recombinant vector and a process for producing a transgenic plant that expresses the CaTLP1 protein are also provided herein.
    Type: Application
    Filed: May 7, 2013
    Publication date: May 7, 2015
    Inventors: Niranjan Chakraborty, Subhra Chakraborty, Asis Datta, Vijay Wardhan, Kishwer Jahan
  • Publication number: 20150121574
    Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
    Type: Application
    Filed: December 19, 2014
    Publication date: April 30, 2015
    Inventors: Erik Dersch, Richard J. Heinzen, Brian J. Martinell, Anatoly Rivlin, Yuechun Wan, Xudong Ye
  • Patent number: 9018449
    Abstract: This invention is directed to a new technique of genetic transformation using low light conditions and leaf strips in the Guayule plant, Parthenium argentatum. The invention also relates to new lines of Guayule, created through this technique.
    Type: Grant
    Filed: March 10, 2004
    Date of Patent: April 28, 2015
    Assignee: The United States of America, represented by The Secretary of Agiculture
    Inventors: Niu Dong, Katrina Cornish
  • Publication number: 20150096080
    Abstract: Disclosed herein is an activation tagging construct for maize, resulting tagged populations and plants. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.
    Type: Application
    Filed: December 15, 2014
    Publication date: April 2, 2015
    Applicant: DOW AGROSCIENCES LLC
    Inventors: John P. Davies, Vaka S. Reddy, William Michael Ainley, D. Ry Wagner, Xing Liang Liu
  • Patent number: 8993839
    Abstract: Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a heterologous protein, infecting plant cells with the Agrobacterium, allowing expression of the heterologous protein, and recovering the heterologous protein from the plant cells. In one embodiment, the protein produced is an endoglucanase.
    Type: Grant
    Filed: July 19, 2011
    Date of Patent: March 31, 2015
    Assignee: The Regents of the University of California
    Inventors: Minsook Hwang, Benjamin E. Lindenmuth, Karen A. McDonald, Abhaya M. Dandekar, Bryce W. Falk, Sang-Kyu Jung, Nathaniel J. Kingsbury
  • Patent number: 8993846
    Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.
    Type: Grant
    Filed: September 5, 2006
    Date of Patent: March 31, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Xudong Ye, Larry A. Gilbertson, Michael W. Petersen
  • Patent number: 8987554
    Abstract: A method is disclosed for reducing the level of gossypol in cottonseed. The method generally includes selectively inducing RNA gene silencing in the seed of a transgenic cotton plant, to interfere with expression of the ?-cadinene synthase gene or the ?-cadinene-8-hydroxylase gene in the seed of the cotton plant without substantially affecting expression of that gene in the foliage, floral parts, and roots of the plant. The transgenic cotton plant comprises at least one of a ?-cadinene synthase gene trigger sequence and/or a ?-cadinene-8-hydroxylase gene trigger sequence operably linked to one or more a seed-specific promoter gene sequences, and the trigger sequence(s) is/are able to induce RNA gene silencing when expressed in cottonseed of the plant.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: March 24, 2015
    Assignee: The Texas A&M University System
    Inventors: Keerti S. Rathore, Ganesan Sunilkumar, LeAnne M. Campbell
  • Patent number: 8975473
    Abstract: The novel constructs and methods of this invention improve tolerance in plants to environmental stresses and senescence. Nucleic acids encoding a plant farnesyl transferase are described, as are transgenic plants and seeds incorporating these nucleic acids and proteins. Also provided are non-naturally occurring mutations in the gene encoding farnesyl transferase which enhance drought tolerance in the plants, improve resistance to senescence and modify growth habit.
    Type: Grant
    Filed: December 9, 2008
    Date of Patent: March 10, 2015
    Assignee: Performance Plants, Inc.
    Inventors: Peter McCourt, Majid Ghassemian, Sean Cutler, Dario Bonetta
  • Publication number: 20150052638
    Abstract: A process of transiently transfecting a plant or leaves on a plant, comprising contacting said plant or said leaves with a suspension comprising Agrobacterium cells of strain CryX or a derivative strain of strain CryX, wherein said derivative strain has the chromosomal background of strain CryX or said derivative strain contains the vir plasmid of strain CryX or a derivative of said vir plasmid.
    Type: Application
    Filed: April 3, 2013
    Publication date: February 19, 2015
    Inventors: Patrick Roemer, Luisa Bortesi, Doreen Tiede, Anatoli Giritch, Yuri Gleba
  • Patent number: 8952219
    Abstract: The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with an Agrobacterium inoculation suspension, said Agrobacterium comprising a nucleic acid of interest, to obtain an Agrobacterium-inoculated sugar cane tissue or cell thereof; b) co-cultivating said Agrobacterium-inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said Agrobacterium-inoculated sugar cane tissue or cell thereof; and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: February 10, 2015
    Assignees: Syngenta Participations AG, Queensland University of Technology
    Inventors: Paulo Cezar De Lucca, Shujie Dong, Robert Jason Christopher Geijskes, Erik Martin Dunder, Manuel Benito Sainz
  • Publication number: 20150040270
    Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.
    Type: Application
    Filed: October 16, 2014
    Publication date: February 5, 2015
    Inventors: Larry Gilbertson, Jason Ward, Jianping Xu, Jon Lamb
  • Patent number: 8940963
    Abstract: Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by (i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or (ii) providing to said plant, said plant tissue or said plant cells a first and a second plus-sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non-competing viral vectors.
    Type: Grant
    Filed: January 27, 2006
    Date of Patent: January 27, 2015
    Assignee: Icon Genetics GmbH
    Inventors: Anatoly Giritch, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
  • Patent number: 8936937
    Abstract: Modified expression vectors, including Tobacco Mosaic Virus (TMV) expression vectors, methods for modifying such vectors, and uses of the same are disclosed.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: January 20, 2015
    Assignee: The Ohio State University Research Foundation
    Inventor: John A. Lindbo
  • Publication number: 20150013034
    Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
    Type: Application
    Filed: September 11, 2014
    Publication date: January 8, 2015
    Inventors: Jyoti R. Rout, Brenda Lowe, John Purcell, Anne Spelletich, Michael Spencer, Melissa Way
  • Patent number: 8927808
    Abstract: The present invention is drawn to novel genes from wild plants, such as wild potato and pepper plants, that confer potyvirus resistance to plants, such as in transformed cultivated plants. Also encompassed are cultivated plants transformed with the novel gene, food products made from the transformed cultivated plants, and methods for making such plants and food products.
    Type: Grant
    Filed: October 18, 2011
    Date of Patent: January 6, 2015
    Assignee: J.R. Simplot Company
    Inventors: Caius Rommens, Hui Duan, Rekha Chawla
  • Patent number: 8921655
    Abstract: The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: December 30, 2014
    Assignee: Joil (S) Pte Ltd.
    Inventors: Jian Ye, Nam Hai Chua, Jing Qu
  • Publication number: 20140373198
    Abstract: The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.
    Type: Application
    Filed: August 18, 2014
    Publication date: December 18, 2014
    Inventors: Patrick S. SCHNABLE, Sudhansu DASH
  • Publication number: 20140366223
    Abstract: This invention provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present invention enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette.
    Type: Application
    Filed: June 6, 2014
    Publication date: December 11, 2014
    Applicant: Syngenta Participations AG
    Inventors: Xianggan Li, Sivamani Elumalai
  • Patent number: 8901372
    Abstract: This invention relates to a nucleic acid construct. The construct includes a nucleic acid molecule configured to silence Banana bunchy top virus (BBTV), a 5? DNA promoter sequence, and a 3? terminator sequence. The nucleic acid molecule, the promoter, and the terminator are operatively coupled to permit transcription of the nucleic acid molecule. The present invention also relates to expression vectors, host cells, and transgenic plants containing the nucleic acid construct of the present invention. Also disclosed are methods of imparting BBTV resistance to plants.
    Type: Grant
    Filed: February 25, 2010
    Date of Patent: December 2, 2014
    Assignee: University of Hawaii
    Inventors: John Hu, Wayne Borth, Eden A. Perez, Kheng Cheah
  • Patent number: 8901377
    Abstract: The present invention relates to methods and compositions for efficient regeneration and transformation of sunflower plants. The invention discloses an efficient method for Agrobacterium-mediated transformation and regeneration of mature, fertile sunflower plants.
    Type: Grant
    Filed: November 11, 2011
    Date of Patent: December 2, 2014
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Thimmaraju Rudrappa, Prakash V. Girhepuje
  • Patent number: 8895808
    Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: November 25, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Larry A. Gilbertson, Susan J. Johnson
  • Patent number: 8889949
    Abstract: The present invention relates to a method for increasing resistance of monocot plants against abiotic stress which comprises a step of transforming monocot plants with a recombinant plasmid containing a fused gene (TPSP) of trehalose-6-phosphate synthetase (TPS) gene and trehalose-6-phosphate phosphatase (TPP) gene to express the TPSP gene while maintaining normal growth and development characteristics. The present invention can increase the resistance of monocot plants against various stresses so that it can greatly contribute to the improvement of production and quality of valuable agricultural crops. The present invention also relates to a transgenic monocot plant, plant cell, or protoplast transformed with a nucleic acid encoding an enzyme for trehalose biosynthesis, under control of an inducible promoter, that increases tolerance to low temperature, salt, and water stress.
    Type: Grant
    Filed: January 3, 2007
    Date of Patent: November 18, 2014
    Assignees: Cornell Research Foundation, Inc., Myongji University Industry and Academia Cooperation Foundation
    Inventors: Ray J. Wu, Ajay K. Garg, Ju-Kon Kim, Baek-Hie Nahm, Yang-Do Choi, In-Cheol Jang, Won-Bin Choi, Yeon-Seak Kim, Chung-Ho Kim, Sang-Ik Song
  • Patent number: 8884101
    Abstract: The method of the present invention includes the step of excising one or more portions selected from a radicle, a germ, and an embryonic axis of a plant tissue inoculated with Agrobacterium after cultivation in a coculture medium. The present invention provides a method of gene introduction that can transform a Triticum plant at high efficiency compared to conventionally known Agrobacterium methods, and provides a method of producing a transformed plant.
    Type: Grant
    Filed: July 29, 2010
    Date of Patent: November 11, 2014
    Assignee: Japan Tobacco Inc.
    Inventors: Yuji Ishida, Yukoh Hiei
  • Patent number: 8865971
    Abstract: Methods for producing homozygous plants, seeds, and plant cells are provided. The methods comprise transforming a somatic cell of a maize haploid embryo with a polynucleotide of interest and treating the transformed cell with a chromosome doubling agent. One or more growth stimulation proteins, such as, for example, RepA or Lec1, may also be provided.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: October 21, 2014
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Zuo-Yu Zhao, Dennis Bidney, Evan Dale Elsing, William James Gordon-Kamm, Michael D Miller, Xinli Wu
  • Patent number: 8865969
    Abstract: According to the present invention, a technique of increasing the frequency of genetic recombination in genomic DNA of a plant is established. Such technique comprises: introducing a restriction enzyme gene that can be expressed in a target plant cell into the plant cell and causing the restriction enzyme gene to be transiently expressed so as to induce genetic recombination of genomic DNA; or introducing a promoter and a restriction enzyme gene using the Agrobacterium method so as to induce genetic recombination of genomic DNA.
    Type: Grant
    Filed: January 18, 2011
    Date of Patent: October 21, 2014
    Assignees: Toyota Jidosha Kabushiki Kaisha, Riken
    Inventors: Satoshi Kondo, Chikara Ohto, Kunihiro Ohta, Shuichi Ohsato, Norihiro Mitsukawa, Nobuhiko Muramoto, Hiroki Sugimoto
  • Patent number: 8853493
    Abstract: The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.
    Type: Grant
    Filed: June 10, 2010
    Date of Patent: October 7, 2014
    Assignee: Temasek Life Sciences Laboratory Limited
    Inventors: Jian Ye, Nam Hai Chua, Jing Qu, Yun Feng Geng, Yun Ping Bu
  • Publication number: 20140296494
    Abstract: The present invention relates to protein expression in plants, particularly the large-scale production of recombinant polypeptides in whole Nicotiana tabacum plants. The use of preselected combination of N. tabacum varieties and Agrobacterium strains, optionally including one or more improvements to the transient expression-based methods of the invention, enables the production of large quantities of a heterologous polypeptides economically and in a short period of time.
    Type: Application
    Filed: January 17, 2012
    Publication date: October 2, 2014
    Applicant: PHILIP MORRIS PRODUCTS S.A.
    Inventors: Julien Beuchat, Prisca Campanoni, Shunhong Dai, Claudio Facchinetti, Nicolas Lugon-Moulin, Karen Oishi, Gustavo Ramirez, Sandrine Roesti, Helene Laparra, Rosa Cabrera
  • Patent number: 8847009
    Abstract: The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.
    Type: Grant
    Filed: August 22, 2013
    Date of Patent: September 30, 2014
    Assignee: Monsanto Technology LLC
    Inventor: Jyoti R. Rout
  • Patent number: 8829275
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Grant
    Filed: July 25, 2012
    Date of Patent: September 9, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Xudong Ye, Michael W. Petersen, Larry Gilbertson, David Walters, Susan Johnson, Shihshieh Huang, Paul S. Chomet
  • Publication number: 20140237688
    Abstract: The present invention relates to an improved method of producing a transgenic plant. Said method comprises, inter alia, the steps of a) providing a wounded transformable explant comprising a hypocotyl or a portion thereof, at least one cotyledon and wounded tissue, b) transforming cells comprised by said explant, and c) transferring said explant to a growing medium, comprising at least one selection compound for a selectable marker, by inserting the hypocotyl of said explant into said growing medium. Moreover, the present invention relates to a plant obtainable by the method according to the present invention.
    Type: Application
    Filed: July 20, 2012
    Publication date: August 21, 2014
    Inventors: Yin-Fu Chang, Aparna Sri Vanguri, Leslie Grist, Holly Tuttle, Hai Ping Hong, Paula Olhoft
  • Patent number: 8779238
    Abstract: The present invention provides methods for transforming Camelina plants. In particular, the present invention relates to transforming Camelina sativa plants through contacting the plants to a dipping solution comprising Agrobacterium, a sugar, and a nonionic surfactant. The methods do not require a vacuum filtration step. The present invention provides, for example, useful methods for developing transformation systems for Camelina sativa that can enable manipulation of its agronomic qualities.
    Type: Grant
    Filed: March 19, 2009
    Date of Patent: July 15, 2014
    Assignee: Global Clean Energy Holdings, Inc.
    Inventors: Thu Nguyen, Xunjia Liu, Jay Derocher