Abstract: Certain exemplary embodiments can provide a system comprising a hybrid composite. The hybrid composite can comprise tubular carbon and graphene produced via pyrolysis of a milled solid carbon source under an unoxidizing environment. When analyzed via X-ray diffraction, the hybrid composite can generate peaks at two theta values of approximately 26.5 degrees, approximately 42.5 degrees, and/or approximately 54.5 degrees.

Abstract: Provided are high-throughput detection systems. The systems include a magnetic sensor device, a magnetic field source and a reservoir plate that includes a plurality of fluid reservoirs. The magnetic sensor device includes a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end. Also provided are methods in which the subject high-throughput detection systems find use.

Abstract: Multi-leg luminescent nanoparticles (“MLN's”) that can be paired to other MLN's as well s biological molecules to film branched multi-leg luminescent nanoparticles (“BMLN's) that can be used in biological multiplexing applications, imaging applications, biological detection applications and other biological applications.

Abstract: Provided is a sensing device, which includes a reactive material layer (260) responding to a specific functional group in a fluid, a sensing capacitor (B) including first and second electrodes disposed on and under an insulating layer (230), the first electrode being disposed under the reactive material layer (260), and a field effect transistor including a gate electrode connected with the first electrode of the sensing capacitor. Here, the reactive material layer (260) is formed in a conductive three-dimensional structure to widen a surface area. Thus, the sensing device may have high sensitivity by maximizing a capacitor sharing effect and a change in voltage amount applied to a gate, which may be caused by widening a surface area of the conductive three-dimensional structure with respect to the fluid flow.

Abstract: Disclosed herein are reagents that include a moiety that includes a metal such as titanium and that readily binds to phosphorylated molecules the reagents also include at least one moiety that produces a signal or that binds to a molecule that produces a signal. The reagent may also include a moiety that binds to a larger molecule or to a surface. Some forms of the reagent include a dendrimer that can simultaneously bind to multiple metal moieties that include a metal such as titanium and multiple moieties that can be used to detected bound molecules. These reagents can be used in detection and/or measurement and/or at least partial purification of phosphorylated molecules. These reagents and methods using them are used to analyze proteins, polypeptides, nucleic acids, phospholipids and the like. They are readily adapted for use in gels, blots, plate based high through put assays and for mass spectrometry.

Abstract: Fluorescent bioprobes comprising fluorogen formed nanoparticles comprising fluorogens with aggregation-induced emission (ATE) properties, which can be used for long-term cell tracking. The fluorogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution. The fluorescent molecules can readily pass through cell membranes, stain only the cell cytoplasm, and form highly emissive nanoaggregates in aqueous media without any obvious cytoxicity in the living cells. Furthermore, the molecules can be retained inside the cells without noticeable leakage to the outside. Therefore, these ATE-based compounds can be used as selective and cell-compatible fluroescent bioprobes for long-term live cell tracking and imaging.

Abstract: A kit for dot immunogold directed filtration assay including a dot immunogold directed filtration card, a detection probe labeled by nano colloidal gold or latex beads, a negative standard, a positive standard, and a cleaning solution.

Abstract: Techniques and systems are disclosed for detecting biomolecular interactions based on the motion of nanomotors. In one aspect, a method of detecting biomolecular interactions based on a motion of a nanomachine includes functionalizing a nanomachine with a capture probe adapted to interact with biological targets; and detecting a presence of the biological targets in an environment based on a motion of the nanomachine.

Abstract: A sensor scheme combining nano-photonics and nano-fluidics on a single platform through the use of free-standing photonic crystals is described. By harnessing nano-scale openings, both fluidics and light can be manipulated at sub-wavelength scales. The convective flow is actively steered through the nanohole openings for effective delivery of the analytes to the sensor surface, and refractive index changes are detected in aqueous solutions. Systems and methods using cross-polarization measurements to further improve the detection limit by increasing the signal-to-noise ratio are also described.

Abstract: The present invention relates to a method of rapidly detecting microorganisms using nanoparticles, and more particularly to a method and device of rapidly detecting microorganisms by adding, to the microorganisms to be detected, nanoparticles having immobilized thereon an antibody that binds specifically to the microorganisms to be detected, subjecting the mixture to an immune reaction to form a reaction solution, passing the reaction solution through a microorganism-concentrating film to concentrate the microorganisms, capturing microorganisms, which was immune-reacted with the antibody-immobilized nanoparticles, by a microorganism-capturing filtration membrane, and determining the presence and concentration of the microorganisms.

Abstract: The present invention provides a method for detecting and grading benign and malignant tumors using at least one sensor of conductive nanoparticles capped with an organic coating in conjunction with a learning and pattern recognition algorithm. The method utilizes a plurality of response induced parameters to obtain improved sensitivity and selectivity for diagnosis, prognosis, monitoring and staging various types of cancers, or for identifying or grading benign or malignant tumors.

Abstract: The present invention is related to fragments of human melanotransferrin (p97). In particular, this invention relates to treatment of diseases through the introduction of the melanotransferrin fragment conjugated to a therapeutic or diagnostic agent to a subject.

Abstract: Included in the disclosure is description of a method of determining a presence, and/or aggressiveness level, and/or response to therapy of a human disease in a subject.

Abstract: A flexible method for making multiplexed assays for antigen-specific antibody responses is disclosed herein. The method of the present invention comprises incubation of a substrate or a set of beads coated with a dockerin or a cohesin protein with a cohesin or dockerin-antigen fusion proteins to attach the fusion protein essentially irreversibly by non-covalent interaction. The present invention enables the use of one dockerin- or cohesin-coated bead for multiple sets of cohesin- or dockerin-antigens, obviating the need to directly chemically couple new antigens to new beads.

Abstract: This invention relates to a method for detecting an analyte in a sample. The method comprises the steps of exposing the sample to a transducer having a pyroelectric or piezoelectric element and electrodes which is capable of transducing a change in energy to an electrical signal, the transducer having at least one reagent proximal thereto, the reagent having a binding site which is capable of binding the analyte or a complex or derivative of the analyte, wherein at least one of the analyte or the complex or derivative of the analyte has a label attached thereto which is capable of absorbing the electromagnetic radiation generated by the radiation source to generate energy by non-radiative decay; irradiating the reagent with a series of pulses of electromagnetic radiation, transducing the energy generated into an electrical signal, detecting the electrical signal and the time delay between each pulse of electromagnetic radiation from the radiation source and the generation of the electric signal.

Abstract: A method of one reaction step for synthesis of peptide template fluorescent metal nanoclusters as bioprobes. Specific targeting peptide containing a metal reactive group is synthesized and used to react with a metal salt solution under a sufficient pH condition at room temperature in forming peptide template fluorescent metal nanoclusters. The dialyzed metal nanoclusters is used directly as bio-probes.

Abstract: There is provided a biosensor for detecting a thiol group and a method of manufacturing the biosensor. In detail, in the method, Au nano particles are manufactured by irradiating radiation (Step 1), a PTh-EDOT/ITO film is manufactured by forming a poly(thiophene-co-3,4-ethylenedioxythiophene) (PTh-EDOT) layer on an indium tin oxide (ITO) coated substrate using cyclic voltammetry (CV) (Step 2) (Step 2); and a Au nano particle modified PTh-EDOT/ITO film is manufactured by dispersing the Au nano particles manufactured in Step 1 onto the PTh-EDOT/ITO film manufactured in Step 2 (Step 3).

Abstract: A multi-pillar structure for molecular analysis is provided. The structure comprises at least two nanopoles, each nanopole attached at one end to a substrate and freely movable along its length. The opposite ends of the at least two nanopoles are each capable of movement toward each other to trap at least one analyte molecule at their opposite ends. Each nanopole is coated with a metal coating. An array of such multi-pillar structures is also provided. A method for preparing the multi-pillar structure is further provided.

Abstract: Passivated semiconductor nanoparticles and methods for the fabrication and use of passivated semiconductor nanoparticles is provided herein.

Abstract: Novel fluorescent dye comprising metal oxide nanoparticles are prepared where the nanoparticles are as small as 3 nm or up to 7000 nm in diameter and where the dye is bound within the metal oxide matrix. In some embodiments the invention, novel dyes are covalently attached to the matrix and in other embodiments of the invention a dye is coordinate or ionic bound within the metal oxide matrix. A method for preparing the novel covalently bondable modified fluorescent dyes is presented. A method to prepare silica comprising nanoparticles that are 3 to 8 nm in diameter is presented. In some embodiments, the fluorescent dye comprising metal oxide nanoparticles are further decorated with functionality for use as multimodal in vitro or in vivo imaging agents. In other embodiments of the invention, the fluorescent dye comprising metal oxide nanoparticles provide therapeutic activity and incorporated therapeutic temperature monitoring.

Abstract: Embodiments herein report compositions, systems and methods for making and using plasmid vectors and nanotube complexes. In certain embodiments, compositions, systems and methods herein include making plasmid vectors having aptamer inserts. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of plasmid vectors having aptamer inserts directed to a particular target agent. Other aspects concern plasmid constructs associated with organic semiconductors. Yet other aspects concern complexes of nanotubes associated with dsDNA aptamers and tracking molecules.

Abstract: Methods for the detection/quantification of the enzymatic activity of hydrolases in a sample, wherein the hydrolase catalyses a hydrolysis reaction of a substrate which yields either H2S or a thiol-containing organic compound that produces a decomposition reaction which generates H2S, and methods for the detection/quantification of their substrates in a sample, as well as a method for the preparation of fluorescent CdS quantum dots, the preparation method, comprising first carrying out a enzymatic reaction catalysed by the hydrolase wherein the hydrolase catalyses the hydrolysis reaction of a substrate which yields either H2S or a thiol-containing organic compound that produces a decomposition reaction which generates H2S, and then reacting the resulting H2S with a salt of Cd+2, thereby fluorescent CdS quantum dots are obtained.

Abstract: Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

Abstract: The present invention provides a system and method for diagnosing, monitoring or prognosing Multiple Sclerosis at different stages as well as affording the prediction of disease activity and response to a treatment regimen, using at least one sensor comprising carbon nanotubes or metal nanoparticles, each coated with various organic coatings in conjunction with a pattern recognition algorithm.

Abstract: Apparatus and methods for detecting an a biomarker indicative of an inflammatory condition, including a capillary tube adapted for one or more biomarkers to adhere to an interior surface thereof, a light source for energizing quantum dots conjugated with the biomarkers within the capillary tube, and a detection system for detecting and quantifying fluorescent energy emitted by the quantum dots in one or more predetermined wavelength ranges, each wavelength range being correlated to one and only one of the biomarkers. A method of stabilizing the fluorescence intensity of quantum dots is also disclosed.

Abstract: The present invention provides a system for measuring biomarker analytes indicative of various diseases comprising an array of sensors sensitive to volatile organic compounds. Particularly, the system is composed of a random network of single-walled carbon nanotubes (SWCNTs) coated with non-polar small organic molecules in conjunction with learning and pattern recognition algorithms. Methods of discriminating between breath samples of healthy individuals and of lung cancer patients are disclosed.

Abstract: Provided are a multiple analysis device and a method of analyzing cancer cells in blood using the device. In this device and method, it can analyze the cancer cells along cancer kinds by using the magnetic nanoparticles combined to the markers of the cancer cells and the difference of the magnetic fields of them.

Abstract: A method of preparing an antigen-immobilized immuno-fluorescence slide, the method comprising: immobilizing a C-reactive protein on a slide to prepare a protein chip; mixing an antibody that specifically binds to a target protein, with streptavidin to label the antibody with a fluorescent nanoparticle; immuno-reacting the antibody by competitive mixing, assaying with a fluorescence camera, wherein the immobilizing of the C-reactive protein on the slide comprises: modifying the slide with 3-aminopropyltrimethoxysilane to prepare a modified slide; hydrating the slide modified with 3-aminopropyltrimethoxysilane; activating the modified slide by using a glutaraldehyde solution; dissolving a C-reactive protein at a concentration of 0.01-0.5 mg/ml in a 30-70 mM phosphate buffer solution (pH 6.5-7.

Abstract: Methods and systems for using drugs as biomarkers to investigate the status of biological systems are disclosed. A drug is conjugated with a light emitting dye that emits light in a channel of the electromagnetic spectrum when an appropriate stimulus is applied. In one aspect, a suspension suspected of containing target particles is added to a tube along with the conjugated drug/dye complex and a float. Centrifugation of the tube, float, and suspension causes various components to separate along the axial length of the tube. Binding of the drug/dye complex to the target particles can be assessed by applying an appropriate stimulus to the tube, which, in turn, causes the fluorescent dyes to emit light in the channel. The level of fluorescence of the target particles located between the float and the inner wall of the tube can be used to assess use of the drug in therapy.

Abstract: Systems and methods that facilitate magnetic collection and/or manipulation of individual micropallets are provided. The embodiments provided herein are directed to a new method for collecting micropallets once released from a substrate. It is accomplished by endowing the micropallets with magnetic properties by incorporating ferromagnetic or superparamagnetic nanoparticles into the photoresist material or otherwise incorporating magnetically responsive material into the micropallet structure. The magnetic particles, which posses magnetic qualities, e.g., ferromagnetism, ferrimagnetism, paramagnetism, and are composed of iron, nickel, and/or other magnetic materials, are mixed into the bulk photoresist prior to its use in the fabrication of microstructures. Also covered are other methods of incorporating magnetically-attractable material into the micropallet structure, such as plating of the micropallets with a material that is magnetically responsive, such as nickel.

Abstract: The present invention relates to method detecting analytes by surface enhanced Raman spectroscopy (SERS), comprising contacting the analytes with at least one analyte binding molecule attached to a metal substrate surface that enhances Raman scattering via a Raman-active molecular linker; and detecting a surface enhanced Raman signal from said compound. In a further aspect, this invention relates to a conjugate and a biosensor suitable for the invented SERS-based analyte detection method.

Abstract: Sensor platforms and methods of making them are described. A platform having a non-horizontally oriented sensor element comprising one or more nanostructures such as nanotubes is described. Under certain embodiments, a sensor element has or is made to have an affinity for an analyte. Under certain embodiments, such a sensor element comprises one or more pristine nanotubes. Under certain embodiments, the sensor element comprises derivatized or functionalized nanotubes. Under certain embodiments, a sensor is made by providing a support structure; providing one or more nanotubes on the structure to provide material for a sensor element; and providing circuitry to electrically sense the sensor element's electrical characterization. Under certain embodiments, the sensor element comprises pre-derivatized or pre-functionalized nanotubes. Under other embodiments, sensor material is derivatized or functionalized after provision on the structure or after patterning.

Abstract: Modified western blot membranes with silver nanoparticle allow the small peptides of the NS1 protein of the poultry influenza virus to be kept in the membrane and not to diffuse during transferring from the Tricine SDS PAGE. These peptides may differentiate infected from vaccinated poultry.

Abstract: A dynamic and noninvasive method of monitoring the adhesion and proliferation of biological cells through multimode operation (acoustic and optical) using a ZnO nanostructure-modified quartz crystal microbalance (ZnOnano-QCM) biosensor is disclosed.

Abstract: Point-of-care tools for screening biological samples for markers associated with pathogenic microbial infections. In particular, devices and systems for screening cervical cells for the expression of proteins, which occur as a result of human papillomavirus infection and progression to invasive cervical cancer.

Abstract: There is provided a nanohair structure with the nanowires exposed on a nanotemplate; the method thereof; and a three-dimensional nanostructure-based sensor with ultra-sensitivity and greatly increased three-dimensional surface-to-volume ratio which immobilizes bio-nanoparticles to the nanohair structure and arranges antibodies to the nano surface with the controlled orientation by physical interaction.

Abstract: An electrochemical paper-immunosensor and method for detecting aminoglycoside antibiotics is developed in the present invention. Single-walled carbon nanotubes are coated on the common filtration paper by dip-dry cycles. With antibody against neomycin adding to the coating solution, a high sensitive biosensor for specific detection of neomycin is prepared, satisfying to the rigid authority regulations. The sensor is not only sensitive but also rapid, comparing with the classic ELISA method, with LOD of 0.04 ng mL?1 and the whole detection process lasting less than 30 min. Another notable advantage of this invention is the versatility of the sensor, similar method is engaged to prepare the versatile sensor for other aminoglycoside antibiotics, replacement with relevant antibodies.

Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

Abstract: The present invention relates to immunoassay methods for measurements of concentrations of related analytes in samples of body fluids from different mammalian species. More particularly, it provides reagents and methods that may be used for assays of a mammalian antigen across a range of different mammalian species, using immunological reagents based on non-mammalian antibodies towards said mammalian antigen. Moreover, a calibrator or a set of calibrators is provided, assigned with concentration values for the said antigen for each of the different mammalian species. The advantage of this invention is that the analytical laboratories or veterinarians may use the same reagents and method to test a plurality of different mammalian species without having to use different immunoassay reagents for different mammalian species.

Abstract: The present invention relates to thiol- or an amine-associated ferromagnetic or superparamagnetic copper nanoparticles with an average diameter less than 30 nm, to the method for obtaining them and their applications in biomedicine and other fields.

Abstract: The disclosure relates to the detection of analytes (e.g., biological pathogens such as bacteria or viruses) using a conductive polymer label. The disclosed detection system utilizing the conductive polymer label generally involves the formation of an analyte conjugate between the target analyte and a conductive polymer moiety conjugated to the target analyte. The conductive polymer portion of the analyte conjugate is electrically activated to form an electrically activated analyte conjugate having an increased electrical conductivity relative to the analyte conjugate as originally formed. The electrically activated analyte conjugate can then be detected by any suitable means, such as by conductimetric or electrochemical detection.

Abstract: Embodiments in accordance with the present invention relate to packed-column nano-liquid chromatography (nano-LC) systems integrated on-chip, and methods for producing and using same. The microfabricated chip includes a column, flits/filters, an injector, and a detector, fabricated in a process compatible with those conventionally utilized to form integrated circuits. The column can be packed with supports for various different stationary phases to allow performance of different forms of nano-LC, including but not limited to reversed-phase, normal-phase, adsorption, size-exclusion, affinity, and ion chromatography. A cross-channel injector injects a nanolitre/picolitre-volume sample plug at the column inlet. An electrochemical/conductivity sensor integrated at the column outlet measures separation signals.

Abstract: The invention relates to systems and methods for improving optical detection and sensitivity in situations in which emission of luminescent light is monitored. It is provided a sample carrier which achieves increased sensitivity of luminescent light detection. The sample carrier comprises a sample carrying part and a light reflecting part; wherein the light reflecting part is positioned to allow an optical collection and detection system to collect not only luminescent light emitted from the sample positioned on the sample carrying part in a direction of the optical collection and detection system, but also luminescent light emitted from the sample in a direction away from the optical collection and detection system and reflected in the direction of the optical collection and detection system via the light reflecting part.

Abstract: The invention relates to the discovery of cancer antigens associated with ovarian cancer. In further aspects, the invention relates to methods, compositions and kits for the diagnosis and treatment of cancer, particularly ovarian and pancreatic cancers.

Abstract: The present disclosure is directed to compositions comprising templated nanoconjugates and methods of their use.

Abstract: Provided are a DNA sequence analysis method of high precision providing improved optical limits by detecting wavelengths of lights emitted from labels in the state where a DNA is electrically tethered and completely stretch, and a nanodevice chip for automating the method. Also provided are a DNA sequence analysis method capable of removing binding errors through complementarily binding between a plurality of peptide nucleic acids (PNAs) labeled with labels emitting lights of different wavelengths and a target DNA to be sequenced, and resolving the limit in optical spatial resolution.

Abstract: The present invention provides simple and inexpensive assays for the detection of virtually any analyte in any sample that is in liquid form or that can be solubilized. The assays utilize the fluid dynamics of drop evaporation whereby soluble materials, including analytes and particles binding thereto, are drawn to the edge of the drop and ultimately form a concentrated residual ring. The presence or absence of certain reagents can then be detected through a number of different approaches.

Abstract: Embodiments disclosed herein relate to a method of detecting specific DNA sequences and the application of this method in the detection of pathogens, viruses, drug-resistant pathogens, genomic variations associated with disease/disorder susceptibility etc. based on specific signature sequences unique to the pathogens, viruses, drug-resistant pathogens or genomic variations. The method can also be used to distinguish a pool of same-sized dsDNA on the basis of sequence differences. The method uses non-optically labeled bis-PNA and/or gamma-PNA probes to tag specific target sequences for identification by solid-state nanopores.

Abstract: A method for magnetic sorting of mammalian sperm cells having damaged membranes is described. In an embodiment of the invention, carboxyl-group functionalized magnetic particles are conjugated to propidium iodide, the resulting composition is mixed with a sample of sperm cells, and sperm cells bound to magnetic particles are separated by magnetic-activated cell sorting.

Abstract: This invention provides targeted nanoclusters comprising multiple polyvalent nanoparticle core units or nanoscaffolds, each nanoparticle core unit attached to multiple targeting moieties and multiple detectable moieties. The nanoclusters find use in a broad range of analytical assays, diagnostic assays and as targeted therapeutics.