Using Nanostructure As Support Of Dna Analysis Patents (Class 977/924)
  • Patent number: 9011774
    Abstract: Provided are a barcode nano-wire for decoding a hard magnetic segment by using highly sensitive magnetic sensors and a bio-sensing system using the barcode nano-wire. Integration of hard magnetic and non-magnetic segments produces the barcode nanowire and magnetic segments are detected using highly sensitive magnetoresistance sensors. The non-magnetic segment uses a non-magnetic material and a specific biomolecule for bioanalysis is immobilized at a specific portion of the barcode nano-wire. The hard magnetic material has an advantage of higher coercivity and high remanence magnetization, which is considered as an important parameter in selecting the material. The hard magnetic segments produce distinguishable strong stray fields for individually detecting segments using conventional magnetic sensors for multiplexed bioanalysis.
    Type: Grant
    Filed: September 16, 2010
    Date of Patent: April 21, 2015
    Assignee: The Industry & Academic Cooperation in Chungnam National University
    Inventors: CheolGi Kim, Vishnubhotla Sudha Rani, Jong-Ryul Jeong, Seok Soo Yoon
  • Patent number: 8999716
    Abstract: Provided herein are artificial membranes of mycolic acids. The membranes may be unsupported or tethered. These membranes are long lived and highly resistant to electroporation, demonstrating their general strength. The mycolic acid membranes are suitable for controlled studies of the mycobacterial outer membrane and can be used in other experiments, such as nanopore analyte translocation experiments.
    Type: Grant
    Filed: August 22, 2012
    Date of Patent: April 7, 2015
    Assignee: University of Washington
    Inventors: Jens Gundlach, Ian M. Derrington, Kyle W. Langford
  • Patent number: 8986524
    Abstract: A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.
    Type: Grant
    Filed: January 27, 2012
    Date of Patent: March 24, 2015
    Assignee: International Business Machines Corporation
    Inventors: Ali Afzali-Ardakani, Stefan Harrer, Binquan Luan, Hongbo Peng, Stephen M. Rossnagel, Ajay K. Royyuru, Gustavo A. Stolovitzky, Philip S. Waggoner
  • Patent number: 8980179
    Abstract: The present invention relates to detection of fluorescence, and more particularly, to the use of fluorescent moieties in proximity to metallic surfaces to change the spatial distribution of fluorescence in an angular dependent manner and detecting emissions at a determined optimal detection angle thereby increasing sensitivity of the detection.
    Type: Grant
    Filed: May 17, 2007
    Date of Patent: March 17, 2015
    Assignee: University of Maryland, Baltimore County
    Inventor: Chris D. Geddes
  • Patent number: 8975095
    Abstract: A technique is provided for base recognition in an integrated device is provided. A target molecule is driven into a nanopore of the integrated device. The integrated device includes a nanowire separated into a left nanowire part and a right nanowire part to form a nanogap in between, a source pad connected to the right nanowire part, a drain pad connected to the left nanowire part, and the nanopore. The source pad, the drain pad, the right nanowire part, the left nanowire part, and the nanogap together form a transistor. The nanogap is part of the nanopore. A transistor current is measured while a single base of the target molecule is in the nanogap of the nanopore, and the single base affects the transistor current. An identity of the single base is determined according to a change in the transistor current.
    Type: Grant
    Filed: May 29, 2013
    Date of Patent: March 10, 2015
    Assignee: International Business Machines Corporation
    Inventors: Shu-Jen Han, Ajay K. Royyuru, Gustavo A. Stolovitzky, Deqiang Wang
  • Patent number: 8969090
    Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures.
    Type: Grant
    Filed: January 4, 2011
    Date of Patent: March 3, 2015
    Assignee: Life Technologies Corporation
    Inventors: Hongye Sun, Eric S. Nordman, Mark F. Oldham, John R. O'Neill, Charles Connell, Umberto Ulmanella, Aldrich N. K. Lau, Theofilos Kotseroglou, Kenneth J. Livak
  • Patent number: 8894796
    Abstract: In one aspect, methods of nanopore formation in solid state membranes are described herein, In some embodiments, a method of forming an aperture comprises providing at least one solid state membrane in a chamber, selecting a first dose of ions sufficient to provide a first aperture of predetermined diameter through the membrane and exposing a surface of the membrane at a first location to the first dose of ions in a focused ion beam having a focal point of diameter less than or equal to about 1 nm to remove material from the membrane at the first location thereby providing the first aperture having the predetermined diameter or substantially the predetermined diameter.
    Type: Grant
    Filed: June 6, 2012
    Date of Patent: November 25, 2014
    Assignees: The University of North Carolina at Greensboro, Carl Zeiss NTS, LLC
    Inventors: Adam R Hall, Jijin Yang, David C Ferranti, Colin A Sanford
  • Publication number: 20140327446
    Abstract: A method of using a sensor comprising a field effect transistor (FET) embedded in a nanopore includes placing the sensor in an electrolyte comprising at least one of biomolecules and deoxyribonucleic acid (DNA); placing an electrode in the electrolyte; applying a gate voltage in the sub-threshold regime to the electrode; applying a drain voltage to a drain of the FET; applying a source voltage to a source of the FET; detecting a change in a drain current in the sensor in response to the at least one of biomolecules and DNA passing through the nanopore.
    Type: Application
    Filed: July 11, 2014
    Publication date: November 6, 2014
    Inventors: Stephen W. Bedell, Christopher D' Emic, Hongbo Peng, Sufi Zafar
  • Publication number: 20140326604
    Abstract: A technique is provided for base recognition in an integrated device is provided. A target molecule is driven into a nanopore of the integrated device. The integrated device includes a nanowire separated into a left nanowire part and a right nanowire part to form a nanogap in between, a source pad connected to the right nanowire part, a drain pad connected to the left nanowire part, and the nanopore. The source pad, the drain pad, the right nanowire part, the left nanowire part, and the nanogap together form a transistor. The nanogap is part of the nanopore. A transistor current is measured while a single base of the target molecule is in the nanogap of the nanopore, and the single base affects the transistor current. An identity of the single base is determined according to a change in the transistor current.
    Type: Application
    Filed: May 29, 2013
    Publication date: November 6, 2014
    Inventors: Shu-Jen Han, Ajay K. Royyuru, Gustavo A. Stolovitzky, Deqiang Wang
  • Publication number: 20140318970
    Abstract: A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged molecule to pass but to block a protein molecule attached to a ligand connecting to the charged molecule, the opening being filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged molecule through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged molecules passing into the opening. The current changes are employed to trigger a bias field increase to cause separation between the ligand and the protein to infer an interaction strength.
    Type: Application
    Filed: August 14, 2013
    Publication date: October 30, 2014
    Applicant: International Business Machines Corporation
    Inventors: BINQUAN LUAN, RUHONG ZHOU
  • Publication number: 20140318962
    Abstract: A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged molecule to pass but to block a protein molecule attached to a ligand connecting to the charged molecule, the opening being filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged molecule through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged molecules passing into the opening. The current changes are employed to trigger a bias field increase to cause separation between the ligand and the protein to infer an interaction strength.
    Type: Application
    Filed: April 30, 2013
    Publication date: October 30, 2014
    Applicant: INTERNATIONAL BUSINESS CORPORATION
    Inventors: Binquan Luan, Ruhong Zhou
  • Patent number: 8845880
    Abstract: The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: September 30, 2014
    Assignee: Genia Technologies, Inc.
    Inventors: Randall W. Davis, Roger J. A. Chen
  • Publication number: 20140174930
    Abstract: A mechanism is provided for ratcheting a double strand molecule. The double strand molecule is driven into a Y-channel of a membrane by a first voltage pulse. The Y-channel includes a stem and branches, and the branches are connected to the stem at a junction. The double strand molecule is slowed at the junction of the Y-channel based on the first voltage pulse being weaker than a force required to break a base pair of the double strand molecule. The double strand molecule is split into a first single strand and a second single strand by driving the double strand molecule into the junction of the Y-channel at a second voltage pulse.
    Type: Application
    Filed: August 20, 2013
    Publication date: June 26, 2014
    Applicant: International Business Machines Corporation
    Inventors: Binquan Luan, Ruhong Zhou
  • Publication number: 20140174929
    Abstract: A mechanism is provided for ratcheting a double strand molecule. The double strand molecule is driven into a Y-channel of a membrane by a first voltage pulse. The Y-channel includes a stem and branches, and the branches are connected to the stem at a junction. The double strand molecule is slowed at the junction of the Y-channel based on the first voltage pulse being weaker than a force required to break a base pair of the double strand molecule. The double strand molecule is split into a first single strand and a second single strand by driving the double strand molecule into the junction of the Y-channel at a second voltage pulse.
    Type: Application
    Filed: December 21, 2012
    Publication date: June 26, 2014
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Binquan Luan, Ruhong Zhou
  • Patent number: 8741380
    Abstract: A fine metal structure having its surface furnished with microprojections of high strength, high precision and large aspect ratio; and a process for producing the fine metal structure free of defects. There is provided a fine metal structure having its surface furnished with microprojections, characterized in that the microprojections have a minimum thickness or minimum diameter ranging from 10 nanometers to 10 micrometers and that the ratio between minimum thickness or minimum diameter (D) of microprojections and height of microprojections (H), H/D, is greater than 1.
    Type: Grant
    Filed: August 20, 2010
    Date of Patent: June 3, 2014
    Assignee: Hitachi, Ltd.
    Inventors: Hiroshi Yoshida, Haruo Akahoshi, Akihiro Miyauchi, Masahiko Ogino
  • Publication number: 20140131202
    Abstract: A mechanism is provided for capturing a molecule via an integrated system. An alternating voltage is applied to a Paul trap device in an electrically conductive solution to generate electric fields. The Paul trap device is integrated with a nanopore device to form the integrated system. Forces from the electric fields of the Paul trap device position the molecule to a nanopore in the nanopore device. A first voltage is applied to the nanopore device to capture the molecule in the nanopore of the nanopore device.
    Type: Application
    Filed: November 13, 2012
    Publication date: May 15, 2014
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Hongbo Peng, Gustavo A. Stolovitzky, Deqiang Wang
  • Patent number: 8724113
    Abstract: A method for forming a nanostructure penetrating a layer and the device made thereof is disclosed. In one aspect, the device has a substrate, a layer present thereon, and a nanostructure penetrating the layer. The nanostructure defines a nanoscale passageway through which a molecule to be analyzed can pass through. The nanostructure has, in cross-sectional view, a substantially triangular shape. This shape is particularly achieved by growth of an epitaxial layer having crystal facets defining tilted sidewalls of the nanostructure. It is highly suitably for use for optical characterization of molecular structure, particularly with surface plasmon enhanced transmission spectroscopy.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: May 13, 2014
    Assignees: IMEC, Katholieke Universiteit Leuven
    Inventors: Kai Cheng, Pol Van Dorpe, Liesbet Lagae, Gustaaf Borghs, Chang Chen
  • Patent number: 8702944
    Abstract: A method for wetting a nanopore device includes filling a first cavity of the nanopore device with a first buffer solution having a first potential hydrogen (pH) value, filling a second cavity of the nanopore device with a second buffer solution having a second pH value, wherein the nanopore device includes a transistor portion having a first surface, an opposing second surface, and an orifice communicative with the first surface and the second surface, the first surface partially defining the first cavity, the second surface partially defining the second cavity, applying a voltage in the nanopore device, and measuring a current in the nanopore device, the current having a current path partially defined by the first cavity, the second cavity, and the orifice.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: April 22, 2014
    Assignee: International Business Machines Corporation
    Inventor: Venkat K. Balagurusamy
  • Patent number: 8702930
    Abstract: A sensor chip is a sensor device for measuring a property of a substance by adsorbing the substance on a surface of the sensor chip. The sensor chip includes a diaphragm having a first surface, a second surface, and at least one through hole penetrating from the first surface to the second surface. At least a part of the first surface, the second surface, and an inner wall surface of the through hole is covered with a noncrystalline solid layer including SiOX as a main component, in which substance X is an element having higher electronegativity than that of silicon.
    Type: Grant
    Filed: January 11, 2012
    Date of Patent: April 22, 2014
    Assignee: Panasonic Corporation
    Inventors: Kiyoshi Hashimotodani, Yusuke Nakano, Masaya Nakatani, Takeki Yamamoto, Yoshiki Yamada, Takuya Oka, Hiroshi Ushio
  • Patent number: 8683672
    Abstract: A gas sensing device (nanosensor) includes a substrate with at least a pair of conductive electrodes spaced apart by a gap, and an electrochemically functionalized semiconductive nanomaterial bridging the gap between the electrodes to form a nanostructure network. The nanomaterial may be single-walled carbon nanotubes (SWNTs) functionalized by the deposition of nanoparticles selected from the group consisting of an elemental metal (e.g., gold or palladium), a doped polymer (e.g., camphor-sulfonic acid doped polyaniline), and a metal oxide (e.g. tin oxide). Depending on the nanoparticles employed in the functionalization, the nanosensor may be used to detect a selected gas, such as hydrogen, mercury vapor, hydrogen sulfide, nitrogen dioxide, methane, water vapor, and/or ammonia, in a gaseous environment.
    Type: Grant
    Filed: November 8, 2012
    Date of Patent: April 1, 2014
    Assignee: The Regents of the University of California
    Inventors: Marc A. Deshusses, Nosang V. Myung, Wayne Bosze
  • Patent number: 8673621
    Abstract: The present patent application describes a cantilever for atomic force microscopy (AFM), which includes a cantilever body having a fixed end and a free end, the free end having a surface region being chemically modified by a dendron in which a plurality of termini of the branched region of the dendron are bound to the surface, and a terminus of the linear region of the dendron is functionalized.
    Type: Grant
    Filed: February 12, 2007
    Date of Patent: March 18, 2014
    Assignees: POSTECH Foundation, POSCO
    Inventors: Joon Won Park, Yu Jin Jung, Bong Jin Hong, Saul Tendler, Stephanie Allen
  • Patent number: 8641877
    Abstract: Apparatus, system, and method are provided for cutting a linear charged polymer inside a nanopore. A first voltage is applied to create an electric field in a first direction. A second voltage is applied to create an electric field in a second direction, and the first direction is opposite to the second direction. When the electric field in the first direction and the electric field in the second direction are applied to a linear charged polymer inside a nanopore, the linear charged polymer is cut at a location with predetermined accuracy.
    Type: Grant
    Filed: August 9, 2012
    Date of Patent: February 4, 2014
    Assignee: International Business Machines Corporation
    Inventors: Binquan Luan, Glenn J. Martyna, Hongbo Peng, Gustavo A. Stolovitzky
  • Patent number: 8641876
    Abstract: The present invention provides a novel device for biosensing and energy storage. The present invented device comprises an electrode having a nanopore structured and bio-communicationally active cyclodextrin attached thereto. The device has demonstrated robust analytical performances for direct single subtype breast cancer measurements without mediators, or native enzyme, and without antibodies labeling; the device is capable to store energy by direct bio-communication with the living cancer cells at real time is demonstrated. It is beneficial in the health care diagnostic applications.
    Type: Grant
    Filed: November 21, 2011
    Date of Patent: February 4, 2014
    Inventor: Ellen Tuanying Chen
  • Publication number: 20140014513
    Abstract: Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.
    Type: Application
    Filed: May 13, 2013
    Publication date: January 16, 2014
    Applicant: Genia Technologies, inc.
    Inventors: Roger J.A. Chen, Randy Davis
  • Publication number: 20140008225
    Abstract: A method of determining or estimating a nucleotide sequence of a nucleic acid by using a device with a nanopore.
    Type: Application
    Filed: February 21, 2013
    Publication date: January 9, 2014
    Applicant: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Tae-han JEON, Dong-ho LEE, Hee-jeong JEONG, Jeo-young SHIM, Kun-sun EOM
  • Publication number: 20130327645
    Abstract: Methods and devices for sequencing nucleic acids are disclosed herein. Devices are also provided herein for measuring DNA with nano-pores sized to allow DNA to pass through the nano-pore. The capacitance can be measured for the DNA molecule passing through the nano-pore. The capacitance measurements can be correlated to determine the sequence of base pairs passing through the nano-pore to sequence the DNA.
    Type: Application
    Filed: August 14, 2013
    Publication date: December 12, 2013
    Applicant: California Institute of Technology
    Inventors: Sameer Walavalkar, Axel Scherer, Thomas A. Tombrello, Aditya Rajagopal, Andrew P. Homyk, Erika Garcia
  • Patent number: 8574894
    Abstract: A molecular dispenser includes a molecular reservoir comprising a plurality of charged molecules; a nanopore proximate the molecular reservoir, the nanopore comprising a first set of locking electrodes configured to establish an electrostatic potential barrier therein and a second set of locking electrodes configured to establish a second electrostatic potential barrier therein; a first drag electrode arranged within the molecular reservoir; and a control unit, wherein the control unit is configured to apply an oscillating voltage across the first set of locking electrodes and a bias voltage to the first drag electrode to control flow of individually charged molecules from the molecular reservoir through the nanopore.
    Type: Grant
    Filed: September 6, 2012
    Date of Patent: November 5, 2013
    Assignee: International Business Machines Corporation
    Inventor: Ajay K. Royyuru
  • Patent number: 8574892
    Abstract: The methods and apparatus 100 disclosed herein concern DNA sequencing. In some embodiments of the invention, the methods comprise measuring the distance between labeled nucleotides 220, such as nucleotides labeled with bulky groups. The methods may further comprise placing identical template DNA 200 into four reaction chambers 110, 120, 130, 140, each containing a different labeled nucleotide precursor, synthesizing complementary strands 230, 240, 250 and detecting labeled nucleotides 220. The distances between labeled nucleotides 220 may be used to construct 450 distance maps 310, 320, 330, 340 for each type of labeled nucleotide 220. The distance maps 310, 320, 330, 340 may be aligned 520 to obtain a nucleic acid sequence 210. Overlapping data analysis and frequency analysis may be used to construct 450 the distance maps 310, 320, 330, 340 and non-overlapping data analysis may be used to align 520 the distance maps into a sequence 210.
    Type: Grant
    Filed: September 22, 2005
    Date of Patent: November 5, 2013
    Assignee: Intel Corporation
    Inventor: Xing Su
  • Patent number: 8569042
    Abstract: An apparatus includes a substrate and a plurality of DNA oligomers in contact with a top surface of the substrate. The substrate is a polar ferroelectric or a polar compound semiconductor.
    Type: Grant
    Filed: February 23, 2005
    Date of Patent: October 29, 2013
    Assignee: Alcatel Lucent
    Inventors: Aref Chowdhury, Hock Min Ng, Bernard Yurke
  • Publication number: 20130264204
    Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer.
    Type: Application
    Filed: September 14, 2012
    Publication date: October 10, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jon R. Sauer, Bart J. van Zeghbroeck
  • Patent number: 8546080
    Abstract: A method for dispensing charged particles includes applying a bias voltage to promote motion of charged molecules through a nanopore, detecting passage of at least one charged molecule through the nanopore, and manipulating an electrostatic potential barrier inside the nanopore, so as to prevent movement of additional charged molecules through the nanopore.
    Type: Grant
    Filed: June 17, 2011
    Date of Patent: October 1, 2013
    Assignee: International Business Machines Corporation
    Inventor: Ajay K. Royyuru
  • Publication number: 20130233709
    Abstract: Provided is a device comprising an upper chamber, a middle chamber and a lower chamber, wherein the upper chamber is in communication with the middle chamber through a first pore, and the middle chamber is in communication with the lower chamber through a second pore, wherein the first pore and second pore are about 1 nm to about 100 nm in diameter, and are about 10 nm to about 1000 nm apart from each other, and wherein each of the chambers comprises an electrode for connecting to a power supply. Methods of using the device are also provided, in particular for sequencing a polynucleotide.
    Type: Application
    Filed: July 18, 2012
    Publication date: September 12, 2013
    Applicant: THE REGENT OF THE UNIVERSITY OF CALIFORNIA
    Inventors: William Dunbar, Jungsuk Kim
  • Publication number: 20130236880
    Abstract: A gold nanoparticle-based colorimetric assay kit for hepatitis C virus RNA that detects unamplified HCV RNA in clinical specimens using unmodified AuNPs and oligotargeter polynucleotides that bind to HCV RNA. A method for detecting hepatitis C virus comprising contacting a sample suspected of containing hepatitis C virus with a polynucleotide that binds to hepatitis C virus RNA and with gold nanoparticles, detecting the aggregation of nanoparticles, and detecting hepatitis C virus in the sample when the nanoparticles aggregate (solution color becomes blue) in comparison with a control or a negative sample not containing the virus when nanoparticles do not aggregate (solution color remains red).
    Type: Application
    Filed: January 10, 2011
    Publication date: September 12, 2013
    Applicant: American University of Cairo
    Inventors: Sherif Mohamed Shawky Abduo, Hassan Mohamed El-Said Azzazy El-Badawy
  • Publication number: 20130213815
    Abstract: A process for fabricating a nanochannel system using a combination of microelectromechanical system (MEMS) microfabrication techniques and atomic force microscopy (AFM) nanolithography. The nanochannel system, fabricated on either a glass or silicon substrate, has channel heights and widths on the order of single to tens of nanometers. The channel length is in the micrometer range. The nanochannel system is equipped with embedded micro or nanoscale electrodes, positioned along the length of the nanochannel for electron tunneling based characterization of nanoscale particles in the channel. Anodic bonding is used to cap off the nanochannel with a cover chip.
    Type: Application
    Filed: February 15, 2013
    Publication date: August 22, 2013
    Inventors: Chao-Hung Steve Tung, Jin-Woo Kim
  • Patent number: 8512533
    Abstract: A biosensor includes a plurality of electrodes and a receptor. The plurality of electrodes comprises a plurality of carbon nanotubes. The receptor are located between the plurality of electrodes and electrically connected to the plurality of carbon nanotubes of the plurality of electrodes. In addition, the receptor reacts to a measured object to lead current variation which is transmitted by the plurality of electrodes.
    Type: Grant
    Filed: January 18, 2011
    Date of Patent: August 20, 2013
    Assignees: Tsinghua University, Hon Hai Precision Industry Co., Ltd.
    Inventors: Xue-Shen Wang, Qun-Qing Li, Shou-Shan Fan
  • Publication number: 20130190192
    Abstract: Methods and systems for plasmonically enhanced bionanoantennas for tagging, tracking, and locating targets of interest at long distances in both day and nighttime conditions. The nanoantennas are used to tag a target of interest and emit a wavelength to impart a unique biometric signature. The nanoantennas are detectable by selectively harvesting and plasmonically enhancing incident light in the visible region, then upconverting that energy through an activated phosphor.
    Type: Application
    Filed: January 24, 2013
    Publication date: July 25, 2013
    Applicant: SRC, INC.
    Inventor: SRC, INC.
  • Publication number: 20130146457
    Abstract: Provided herein are methods and systems pertaining to sequencing units of analytes using nanopores. In general, arresting constructs are used to modify an analyte such that the modified analyte pauses in the opening of a nanopore. During such a pause, an ion current level is obtained that corresponds to a unit of the analyte. After altering the modified analyte such that the modified analyte advances through the opening, another arresting construct again pauses the analyte, allowing for a second ion current level to be obtained that represents a second unit of the analyte. This process may be repeated until each unit of the analyte is sequenced. Systems for performing such methods are also disclosed.
    Type: Application
    Filed: August 22, 2012
    Publication date: June 13, 2013
    Applicant: UNIVERSITY OF WASHINGTON
    Inventors: Jens Gundlach, Ian M. Derrington, Marcus D. Collins
  • Patent number: 8455260
    Abstract: A method for determining a sequence of a biomolecule, the method including binding a plurality of uniform probes to a biomolecule fragment, creating a collection of binding signatures for the fragment with each binding signature representing a series of distances between binding sites within the fragment, and grouping the binding signatures into a plurality of signature clusters based at least in part on distances between the binding sites in each binding signature. For each binding signature in a first cluster, a potential successor binding signature is selected from signature clusters other than the first signature cluster, and one of the potential successor binding signatures is identified as a successor binding signature. The last two steps are repeated until the successor signature represents a terminal signature, resulting in a sequence of signatures representing at least a portion of the biomolecule.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: June 4, 2013
    Assignee: Massachusetts Institute of Technology
    Inventors: Peter Goldstein, Franco Preparata, Eli Upfal
  • Patent number: 8440403
    Abstract: An apparatus for investigating a molecule comprising a channel provided in a substrate, a metallic moiety capable of plasmon resonance which is associated with the channel in a position suitable for the electromagnetic field produced by the metallic moiety to interact with a molecule passing therethrough, means to induce a molecule to pass through the channel, means to induce surface plasmon resonance in the metallic moiety; and means to detect interaction between the electromagnetic field produced by the metallic moiety and a molecule passing through the channel. Methods of investigating molecules are also provided.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: May 14, 2013
    Assignee: Base4 Innovation Limited
    Inventor: Cameron Frayling
  • Patent number: 8431344
    Abstract: A method of fabrication or manufacture at micrometer and nanometer scale by spatially selective deposition of chemical substances so as to form a solid phase array on a substrate (10) which includes the steps of defining a region (15) on the substrate by forming an electrostatic charge on that region which is different from the electrostatic charge on other regions of the substrate such as by formation of a latent electrostatic image thereon, applying an emulsion to the substrate. The emulsion (16) has an electrically charged discontinuous phase and a component to be selectively deposited carried in or comprising the discontinuous phase. The discontinuous phase of the emulsion is attracted to the preselected region by attraction by the electrostatic charge on the region and deposition obtained with or without reaction. The electrostatic image may be formed by the use of photoconductor.
    Type: Grant
    Filed: June 30, 2004
    Date of Patent: April 30, 2013
    Assignee: Raustech Pty Ltd
    Inventors: Peter John Hastwell, Timothy Mark Kaethner
  • Publication number: 20130068619
    Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer.
    Type: Application
    Filed: September 14, 2012
    Publication date: March 21, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jon R. Sauer, Bart J. van Zeghbroeck
  • Patent number: 8394584
    Abstract: Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.
    Type: Grant
    Filed: December 18, 2009
    Date of Patent: March 12, 2013
    Assignees: The Board of Trustees of the University of Illinois, The Johns Hopkins University
    Inventors: Gregory Timp, Winston Timp, Andrew Feinberg, Utkur Mirsaidov
  • Publication number: 20130022977
    Abstract: The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus.
    Type: Application
    Filed: September 14, 2012
    Publication date: January 24, 2013
    Applicant: SEQUENOM, INC
    Inventors: Stanley Lapidus, John F. Thompson, Doron Lipson, Patrice Milos, J. William Efcavitch, Stanley Letovsky
  • Publication number: 20130015068
    Abstract: Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.
    Type: Application
    Filed: September 15, 2012
    Publication date: January 17, 2013
    Applicant: GENIA TECHNOLOGIES, INC.
    Inventors: Roger Chen, Randy Davis
  • Publication number: 20120326247
    Abstract: A method of forming a nanopore array includes patterning a front layer of a substrate to form front trenches, the substrate including a buried layer disposed between the front layer and a back layer; depositing a membrane layer over the patterned front layer and in the front trenches; patterning the back layer and the buried layer to form back trenches, the back trenches being aligned with the front trenches; forming a plurality of nanopores through the membrane layer; depositing a sacrificial material in the front trenches and the back trenches; depositing front and back insulating layers over the sacrificial material; and heating the sacrificial material to a decomposition temperature of the sacrificial material to remove the sacrificial material and form pairs of front and back channels, wherein the front channel of each channel pair is connected to the back channel of its respective channel pair by an individual nanopore.
    Type: Application
    Filed: September 7, 2012
    Publication date: December 27, 2012
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Bing Dang, Hongbo Peng
  • Publication number: 20120322064
    Abstract: Hybridization probes for hybridizing to the same target nucleic acid are disclosed, the hybridization probes comprising an electrically-active magnetic nanoparticle-labeled detector probe and a capture probe including a conjugating moiety for immobilization. Also disclosed is a biodetection method including the steps of: providing hybridization probes for hybridizing to the same target nucleic acid, the hybridization probes comprising an electrically-active magnetic nanoparticle-labeled detector probe and a capture probe; hybridizing the target nucleic acid with each of the electrically-active magnetic nanoparticle-labeled detector probe and a capture probe in a sample including the target nucleic acid; magnetically separating the hybridized target nucleic acid from the sample; capturing the hybridized target nucleic acid on a substrate through the capture probe; and measuring the oxidation-reduction signal of the electrically-active magnetic nanoparticle-labeled detector probe.
    Type: Application
    Filed: May 22, 2012
    Publication date: December 20, 2012
    Applicant: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY
    Inventors: Evangelyn C. Alocilja, Sudeshna Pal
  • Publication number: 20120322055
    Abstract: A method for dispensing charged particles includes applying a bias voltage to promote motion of charged molecules through a nanopore, detecting passage of at least one charged molecule through the nanopore, and manipulating an electrostatic potential barrier inside the nanopore, so as to prevent movement of additional charged molecules through the nanopore.
    Type: Application
    Filed: June 17, 2011
    Publication date: December 20, 2012
    Applicant: International Business Machines Corporation
    Inventor: Ajay K. Royyuru
  • Publication number: 20120289408
    Abstract: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
    Type: Application
    Filed: February 23, 2012
    Publication date: November 15, 2012
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Kevin Travers, Geoff Otto, Stephen Turner, Cheryl Heiner, Congcong Ma
  • Patent number: 8273532
    Abstract: In a molecular analysis system, there is provided a structure including a nanopore and first and second fluidic reservoirs. The two reservoirs are fluidically connected via the nanopore. A detector is connected to detect molecular species translocation of the nanopore, from one of the two fluidic reservoirs to the other of the two fluidic reservoirs. A controller is connected to generate a control signal to produce conditions at the nanopore to induce the molecular species to re-translocate the nanopore at least once after translocating the nanopore. This enables a method for molecular analysis in which a molecular species is translocated a plurality of times through a nanopore in a structure between two fluidic reservoirs separated by the structure.
    Type: Grant
    Filed: October 2, 2008
    Date of Patent: September 25, 2012
    Assignee: President and Fellows of Harvard College
    Inventors: Marc H. Gershow, Jene A. Golovchenko, Daniel Branton
  • Publication number: 20120193237
    Abstract: A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.
    Type: Application
    Filed: January 27, 2012
    Publication date: August 2, 2012
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Ali Afzali-Ardakani, Stefan Harrer, Binquan Luan, Hongbo Peng, Stephen M. Rossnagel, Ajay K. Royyuru, Gustavo A. Stolovitzky, Philip S. Waggoner