Search Patents
  • Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant DNA, and process for the preparation of thermostable luciferase of firefly
    Patent number: 5229285
    Abstract: The present invention relates to thermostable luciferase of firefly wherein an amino acid at the 217-position of the amino acid sequence of wild-type firefly luciferase or an amino acid equivalent to the amino acid at the 217-position of luciferase of GENJI firefly or HEIKE firefly is converted into a hydrophobic amino acid, a gene encoding said thermostable luciferase, a vector comprising the gene encoding said thermostable luciferase inserted therein, and a process for the preparation of thermostable firefly luciferase comprising use of said vector.
    Type: Grant
    Filed: June 23, 1992
    Date of Patent: July 20, 1993
    Assignee: Kikkoman Corporation
    Inventors: Naoki Kajiyama, Eiichi Nakano
  • COVALENT MODIFICATION AND CONJUGATION OF LUCIFERASE
    Publication number: 20100003701
    Abstract: A process for reversible chemical modification of the luciferase, a process for the covalent conjugation of a reversibly modified luciferase to a chemical moiety (a protein or a binding partner such as biotin or an antibody), a process for reactivation of the reversibly-modified and inactivated luciferase, a process for making the said luciferase conjugates and a bioluminescent assay method that uses covalently conjugated firefly luciferase are taught. The present invention also relates to a composition comprising a reversibly modified luciferase, as well as a composition comprising a reversibly modified luciferase covalently conjugated to a chemical moiety.
    Type: Application
    Filed: August 3, 2009
    Publication date: January 7, 2010
    Applicant: CardioGenics Inc.
    Inventors: Amer Alagic, Pavel Zhelev, Yahia A. Gawad
  • Thermostable luciferase mutant
    Patent number: 7906298
    Abstract: A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis.
    Type: Grant
    Filed: October 26, 1999
    Date of Patent: March 15, 2011
    Assignee: Promega Corporation
    Inventors: David J Squirrell, Melenie J Murphy, Rachel L Price, Christopher R Lowe, Peter J White, Laurence C Tisi, James A H Murray
  • LUCIFERASE MUTANT
    Publication number: 20140186918
    Abstract: A recombinant protein having luciferase activity and at least 60% similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in Photinus pyralis luciferase is mutated as compared to the corresponding wild-type luciferase, such that the luciferase enzyme is able to emit light at a different wavelength as compared to the corresponding wild-type luciferase and/or has enhanced thermostability as compared to the corresponding wild-type luciferase. In general, the residue corresponding to 357 in Photinus pyralis luciferase is changed from an acidic amino acid to a non-acidic amino acid, and preferably an uncharged polar amino acid such as tyrosine. Mutant luciferases in accordance with the invention can produce a large (50 nm) wavelength shift in emitted light and have good thermostability. The resultant colour shift can be reversed by addition of coenzyme A. These properties make the mutant particularly useful in a variety of assays.
    Type: Application
    Filed: February 14, 2014
    Publication date: July 3, 2014
    Applicant: PROMEGA CORPORATION
    Inventors: David James Squirrell, Melenie Jane Murphy, Rachel Louise Price, Peter John White, Tara Louise Willey
  • Bioluminescent assays utilising secreted luciferases
    Patent number: 10364454
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Grant
    Filed: December 30, 2016
    Date of Patent: July 30, 2019
    Assignee: GENE STREAM PTY LTD.
    Inventors: Marco Peter Leu, John Michael Daly
  • Bioluminescent assays utilising secreted luciferases
    Patent number: 8399212
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Grant
    Filed: December 21, 2007
    Date of Patent: March 19, 2013
    Assignee: Gene Stream Pty Ltd
    Inventors: Marco Peter Leu, John Michael Daly
  • BIOLUMINESCENT ASSAYS UTILISING SECRETED LUCIFERASES
    Publication number: 20170211126
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Application
    Filed: December 30, 2016
    Publication date: July 27, 2017
    Applicant: GENE STREAM PTY LTD.
    Inventors: Marco Peter Leu, John Michael Daly
  • Bioluminescent assays utilising secreted luciferases
    Patent number: 9567624
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Grant
    Filed: March 17, 2015
    Date of Patent: February 14, 2017
    Assignee: Gene Stream Pty Ltd.
    Inventors: Marco Peter Leu, John Michael Daly
  • Mutant luciferase of a firefly
    Patent number: 5330906
    Abstract: The present invention provides industrially useful luciferase. Mutant luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce red, orange or green color of light which can not be produced by wild type luciferase. Mutant luciferase can be used to measure ATP accurately in a colored solution such as red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results.
    Type: Grant
    Filed: June 15, 1993
    Date of Patent: July 19, 1994
    Assignee: Kikkoman Corporation
    Inventors: Naoki Kajiyama, Eiichi Nakano
  • MUTANT LUCIFERASE
    Publication number: 20090263880
    Abstract: An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.
    Type: Application
    Filed: January 26, 2007
    Publication date: October 22, 2009
    Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Kosei Kawasaki, Yousuke Morita, Satoru Ohgiya, Yoshihiro Ohmiya, Yasushi Ohyama
  • Bioluminescent assays utilising secreted luciferases
    Patent number: 11078519
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Grant
    Filed: June 13, 2019
    Date of Patent: August 3, 2021
    Assignee: GENE STREAM PTY LTD.
    Inventors: Marco Peter Leu, John Michael Daly
  • Mutant luciferase
    Patent number: 8147842
    Abstract: An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.
    Type: Grant
    Filed: January 26, 2007
    Date of Patent: April 3, 2012
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Kosei Kawasaki, Yousuke Morita, Satoru Ohgiya, Yoshihiro Ohmiya, Yasushi Ohyama
  • Luciferase mutant
    Patent number: 8669087
    Abstract: A recombinant protein having luciferase activity and at least 60% similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in Photinus pyralis luciferase is mutated as compared to the corresponding wild-type luciferase, such that the luciferase enzyme is able to emit light at a different wavelength as compared to the corresponding wild-type luciferase and/or has enhanced thermostability as compared to the corresponding wild-type luciferase. In general, the residue corresponding to 357 in Photinus pyralis luciferase is changed from an acidic amino acid to a non-acidic amino acid and preferably an uncharged polar amino acid such as tyrosine. Mutant luciferases in accordance with the invention can produce a large (50 nm) wavelength shift in emitted light and have good thermostability. The resultant colour shift can be reversed by addition of coenzyme A. These properties make the mutant particularly useful in a variety of assays.
    Type: Grant
    Filed: October 26, 2000
    Date of Patent: March 11, 2014
    Assignee: Promega Corporation
    Inventors: David James Squirrell, Melenie Jane Murphy, Rachel Louise Price, Peter John White, Tara Louise Willey
  • IMPROVED MODIFIED/MUTANT BACTERIAL LUCIFERASES
    Publication number: 20200339964
    Abstract: Modified or mutant bacterial luciferases having improved activity, as compared to wild type or unmodified bacterial luciferases, are described. The modified or mutant bacterial luciferases display increased light production and/or slower signal decay. Employing these modified or mutant bacterial luciferases improve a luminescence reporter system assay by decreasing the detection sensitivity, resulting in improved bioreporter/reporter assays. The invention further provides methods for using the modified or mutant bacterial luciferases, reporter assays using the modified or mutant bacterial luciferases, and kits and articles of manufacture.
    Type: Application
    Filed: October 25, 2018
    Publication date: October 29, 2020
    Inventor: Shawn Lee Suko
  • Luciferase gene and novel recombinant DNA as well as a method of producing luciferase
    Patent number: 4968613
    Abstract: A luciferase gene isolated from Luciola cruciata (Japanese firefly) coding for an amino acid sequence shown in FIG. 4 and a novel recombinant DNA characterized by incorporating a gene coding for luciferase into a vector DNA are disclosed. There is also disclosed a method of producing luciferase which comprises culturing in a medium a microorganism containing a recombinant DNA having inserted a gene coding for luciferase in a vector DNA and belonging to the genus Escherichia capable of producing luciferase and collecting luciferase from the culture.
    Type: Grant
    Filed: July 26, 1988
    Date of Patent: November 6, 1990
    Assignee: Kikkoman Corporation
    Inventors: Tsutomu Masuda, Hiroki Tatsumi, Eiichi Nakano
  • THERMOSTABLE LUCIFERASES AND METHODS OF PRODUCTION
    Publication number: 20120009647
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Application
    Filed: August 19, 2011
    Publication date: January 12, 2012
    Inventors: Keith V. Wood, Mary P. Hall, Monika G. Wood
  • Thermostable luciferases and methods of production
    Publication number: 20060183212
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Application
    Filed: December 1, 2005
    Publication date: August 17, 2006
    Inventors: Keith Wood, Mary Hall, Monika Wood
  • Modified/mutant bacterial luciferases
    Patent number: 11788068
    Abstract: Modified or mutant bacterial luciferases having improved activity, as compared to wild type or unmodified bacterial luciferases, are described. The modified or mutant bacterial luciferases display increased light production and/or slower signal decay. Employing these modified or mutant bacterial luciferases improve a luminescence reporter system assay by increasing the detection sensitivity, resulting in improved bioreporter/reporter assays. The invention further provides methods for using the modified or mutant bacterial luciferases, reporter assays using the modified or mutant bacterial luciferases, and kits and articles of manufacture.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: October 17, 2023
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Shawn Lee Suko
  • SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT
    Publication number: 20220389434
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Application
    Filed: June 20, 2022
    Publication date: December 8, 2022
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • Thermostable luciferases and methods of production
    Patent number: 8822170
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Grant
    Filed: August 19, 2011
    Date of Patent: September 2, 2014
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Monika G. Wood, Mary P. Hall
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