Search Patents
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Publication number: 20080090263Abstract: The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.Type: ApplicationFiled: October 13, 2006Publication date: April 17, 2008Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Yoshihiro Ohmiya, Chun Wu
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Patent number: 8748144Abstract: A Heike firefly luciferase having excellent thermostability and storage stability and a process for its production, wherein the amino acid corresponding to position 287 of Heike firefly luciferase is alanine.Type: GrantFiled: February 16, 2010Date of Patent: June 10, 2014Assignee: Kikkoman CorporationInventors: Yukako Kodama, Eriko Yoshihara
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Patent number: 8669087Abstract: A recombinant protein having luciferase activity and at least 60% similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in Photinus pyralis luciferase is mutated as compared to the corresponding wild-type luciferase, such that the luciferase enzyme is able to emit light at a different wavelength as compared to the corresponding wild-type luciferase and/or has enhanced thermostability as compared to the corresponding wild-type luciferase. In general, the residue corresponding to 357 in Photinus pyralis luciferase is changed from an acidic amino acid to a non-acidic amino acid and preferably an uncharged polar amino acid such as tyrosine. Mutant luciferases in accordance with the invention can produce a large (50 nm) wavelength shift in emitted light and have good thermostability. The resultant colour shift can be reversed by addition of coenzyme A. These properties make the mutant particularly useful in a variety of assays.Type: GrantFiled: October 26, 2000Date of Patent: March 11, 2014Assignee: Promega CorporationInventors: David James Squirrell, Melenie Jane Murphy, Rachel Louise Price, Peter John White, Tara Louise Willey
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Publication number: 20030166905Abstract: The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.Type: ApplicationFiled: June 28, 2002Publication date: September 4, 2003Applicant: Promega CorporationInventors: Keith V. Wood, Monika G. Wood
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Publication number: 20140073031Abstract: The present invention relates to a luciferase derived from a star-worm belonging to genus Diplocladon, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.Type: ApplicationFiled: September 13, 2013Publication date: March 13, 2014Applicants: OLYMPUS CORPORATION, PERAK STATE DEVELOPMENT CORPORATION, NIMURA GENETIC SOLUTIONS CO., LTD.Inventors: Ryutaro AKIYOSHI, Katsunori OGO, Hirobumi SUZUKI
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Publication number: 20120034672Abstract: This invention provides a genetically modified marine luciferase such as Gaussia luciferase, which has high bioluminescence intensity, and has high bioluminescence stability and/or red-shifted wavelength. Specifically disclosed is a luciferase variant with improved optical property obtained by replacing at least one amino acid residue among the amino acid sequence of a marine luciferase at positions corresponding to positions 89 to 118 in the amino acid sequence of Gaussia luciferase (GLuc), wherein an amino acid residue at a position corresponding to at least one selected from positions 89, 90, 95, 97, 100, 108, 112, 115, and 118 in the amino acid sequence of GLuc is replaced by way of conservative amino acid replacement. The above-mentioned replacement in a marine luciferase improves enzymatic activity of the luciferase. Also disclosed is a bioluminescent probe having an improved optical property, which is produced using the luciferase variant of the present invention.Type: ApplicationFiled: October 17, 2011Publication date: February 9, 2012Inventors: SungBae KIM, Hiroaki Tao, Moritoshi Sato
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Patent number: 6552179Abstract: The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.Type: GrantFiled: June 7, 1995Date of Patent: April 22, 2003Assignee: Promega CorporationInventors: Keith V. Wood, Monika G. Gruber
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Patent number: 7723502Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.Type: GrantFiled: October 10, 2008Date of Patent: May 25, 2010Assignee: Marker Gene Technologies, IncInventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
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Publication number: 20130040326Abstract: According to one embodiment, the present invention relates to luciferase derived from Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 580 nm at pH 8, or the luciferase indicating 23.3 times or more of luminescent intensity in comparison to that of Rhodamine 6G.Type: ApplicationFiled: August 20, 2012Publication date: February 14, 2013Applicants: OLYMPUS CORPORATION, PERAK STATE DEVELOPMENT CORPORATION, NIMURA GENETIC SOLUTIONS CO., LTD.Inventors: Katsunori OGO, Ryutaro AKIYOSHI, Hirobumi SUZUKI
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Patent number: 7906298Abstract: A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis.Type: GrantFiled: October 26, 1999Date of Patent: March 15, 2011Assignee: Promega CorporationInventors: David J Squirrell, Melenie J Murphy, Rachel L Price, Christopher R Lowe, Peter J White, Laurence C Tisi, James A H Murray
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Patent number: 6387675Abstract: The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.Type: GrantFiled: June 7, 1995Date of Patent: May 14, 2002Assignee: Promega CorporationInventors: Keith V. Wood, Monika G. Gruber
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Publication number: 20090233320Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.Type: ApplicationFiled: December 9, 2004Publication date: September 17, 2009Applicant: NEC Soft, Ltd.Inventor: Hiromi Takenaka
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Publication number: 20140080199Abstract: An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from Lucidina accensa.Type: ApplicationFiled: September 11, 2013Publication date: March 20, 2014Applicant: OLYMPUS CORPORATIONInventors: Katsunori OGO, Ryutaro AKIYOSHI, Mariko MURAI, Takashi KINEBUCHI, Hirobumi SUZUKI
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Publication number: 20110039257Abstract: A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.Type: ApplicationFiled: April 2, 2007Publication date: February 17, 2011Applicant: Promega CorporationInventors: Brock Binkowski, Frank Fan, Susan Wigdal, Keith V. Wood, Monika G. Wood
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Publication number: 20140087402Abstract: A codon optimized and stabilized luciferase gene and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.Type: ApplicationFiled: December 4, 2012Publication date: March 27, 2014Applicant: Marker Gene Technologies, Inc.Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook, Ying Jiang
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Publication number: 20020081644Abstract: A polynucleotide encoding a secreted form of wild type Renilla luciferase. Also provided is a polynucleotide encoding a secreted modified form of wild type Renilla luciferase. Additionally, the polypeptides encoded by the polynucleotides of the present invention and uses of the polynucleotides and polypeptides of the present invention in biological assays. Also, a stable mammalian packaging cell line which produces retroviruses carrying a polynucleotide encoding a secreted Renilla luciferase.Type: ApplicationFiled: March 14, 2001Publication date: June 27, 2002Inventors: Alan P. Escher, Jingxue Liu
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Publication number: 20030135871Abstract: Native and modified forms of Phrixothrix hirtus red luciferase are described. These native and modified forms of luciferase can be used, for example, as reporter molecules in host cells and/or transgenic animals.Type: ApplicationFiled: August 15, 2002Publication date: July 17, 2003Inventors: Kevin A. Nawotka, Weisheng Zhang
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Patent number: 7871803Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.Type: GrantFiled: December 9, 2004Date of Patent: January 18, 2011Assignee: NEC Soft, Ltd.Inventor: Hiromi Takenaka
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Publication number: 20040142356Abstract: The luxA and luxB genes from P. luminescens which encode for the luciferase protein of the bacterial luciferase system were modified to generate codon-optimized versions that are optimized for expression in mammalian cells. The codon-optimized bacterial luciferase enzyme system genes of the invention can be used to develop a mammalian bioluminescence bioreporter useful in various medical research and diagnostics applications.Type: ApplicationFiled: October 30, 2003Publication date: July 22, 2004Inventors: Stacey Patterson, Rakesh Gupta, Gary Sayler, Hebe Dionisi
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Publication number: 20120035077Abstract: This invention provides modified nucleotide sequences encoding luciferase that have greater expression than wild type luciferase.Type: ApplicationFiled: May 27, 2011Publication date: February 9, 2012Applicant: SwitchGear GenomicsInventors: Nathan D. Trinklein, Shelley Force Aldred