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  • RATIOMETRIC DETECTION OF LUCIFERASE ASSAYS USING A CALIBRATOR LUCIFERASE
    Publication number: 20220348984
    Abstract: The present invention relates to a ratiometric bioluminescent assay for the quantification of an analyte of interest, comprising a detector luciferase that is reactive to a substrate to emit light at a first wavelength and wherein the detector luciferase is responsive to the analyte of interest and a calibrator luciferase which is reactive to the same substrate to emit light at a second wavelength which second wavelength is different from the first wavelength emitted by the detector luciferase. The invention further relates to a method for quantifying an analyte of interest in a sample using the bioluminescent assay of the present invention and wherein the quantification of the analyte of interest is based on the calibrated ratio of measured bioluminescence intensities of the detector luciferase and the calibrator luciferase. Further, the invention relates to the use of the bioluminescent assay of the present invention in a method for quantifying an analyte of interest in a sample.
    Type: Application
    Filed: June 22, 2020
    Publication date: November 3, 2022
    Inventors: Maarten MERKX, Yan NI, Leonardus Josephus Van IJzendoorn
  • Thermostable luciferases and methods of production
    Publication number: 20090311769
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Application
    Filed: July 31, 2009
    Publication date: December 17, 2009
    Applicant: Promega Corporation
    Inventors: Keith V. Wood, Mary P. Hall, Monika G. Wood
  • LUCIFERASE VARIANT
    Publication number: 20220064606
    Abstract: In one embodiment, an object of the present invention is to provide a firefly luciferase having improved thermostability. In one embodiment, the present invention relates to a luciferase mutant having improved thermostability that is a mutant of firefly luciferase comprising an amino acid sequence in which the amino acid residue at the position corresponding to position 393 of SEQ ID NO 1 is substituted, a polynucleotide encoding the luciferase mutant, and a production method of the luciferase mutant.
    Type: Application
    Filed: July 5, 2019
    Publication date: March 3, 2022
    Applicant: Kikkoman Corporation
    Inventors: Kanako HAYASHI, Atsushi ICHIYANAGI
  • SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT
    Publication number: 20140120548
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Application
    Filed: October 14, 2013
    Publication date: May 1, 2014
    Applicant: PROMEGA CORPORATION
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • LUCIFERASE VARIANT
    Publication number: 20210171919
    Abstract: In one embodiment, an object of the present invention is to provide a firefly luciferase having improved thermostability. In one embodiment, the present invention relates to a luciferase mutant having improved thermostability that is a mutant of firefly luciferase comprising an amino acid sequence in which the amino acid residue at the position corresponding to position 393 of SEQ ID NO 1 is substituted, a polynucleotide encoding the luciferase mutant, and a production method of the luciferase mutant.
    Type: Application
    Filed: July 5, 2019
    Publication date: June 10, 2021
    Applicant: Kikkoman Corporation
    Inventors: Kanako HAYASHI, Atsushi ICHIYANAGI
  • SUPERLUMINESCENT LUCIFERASE VARIANT WITH PROLONGED BIOLUMINESCENCE
    Publication number: 20120034672
    Abstract: This invention provides a genetically modified marine luciferase such as Gaussia luciferase, which has high bioluminescence intensity, and has high bioluminescence stability and/or red-shifted wavelength. Specifically disclosed is a luciferase variant with improved optical property obtained by replacing at least one amino acid residue among the amino acid sequence of a marine luciferase at positions corresponding to positions 89 to 118 in the amino acid sequence of Gaussia luciferase (GLuc), wherein an amino acid residue at a position corresponding to at least one selected from positions 89, 90, 95, 97, 100, 108, 112, 115, and 118 in the amino acid sequence of GLuc is replaced by way of conservative amino acid replacement. The above-mentioned replacement in a marine luciferase improves enzymatic activity of the luciferase. Also disclosed is a bioluminescent probe having an improved optical property, which is produced using the luciferase variant of the present invention.
    Type: Application
    Filed: October 17, 2011
    Publication date: February 9, 2012
    Inventors: SungBae KIM, Hiroaki Tao, Moritoshi Sato
  • SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT
    Publication number: 20200248228
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Application
    Filed: March 17, 2020
    Publication date: August 6, 2020
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT
    Publication number: 20190185908
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Application
    Filed: March 6, 2019
    Publication date: June 20, 2019
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • Thermostable luciferases and methods of production
    Publication number: 20090137019
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Application
    Filed: June 12, 2007
    Publication date: May 28, 2009
    Inventors: Keith V. Wood, Mary P. Hall, Monika G. Wood
  • SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT
    Publication number: 20210032676
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Application
    Filed: October 20, 2020
    Publication date: February 4, 2021
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT
    Publication number: 20180051318
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Application
    Filed: September 25, 2017
    Publication date: February 22, 2018
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • Synthetic luciferases with enhanced light output
    Patent number: 10844422
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: March 17, 2020
    Date of Patent: November 24, 2020
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • SPLIT RECOMBINANT LUCIFERASE, AND ANALYSIS METHOD USING THEREOF
    Publication number: 20170183637
    Abstract: Disclosed is a split recombinant protein that includes N-terminal and C-terminal fragments of a firefly luciferase, and a linker peptide. The N-terminal fragment is one of two fragments of a firefly luciferase split into two at a splitting position specific to the firefly luciferase. The C-terminal fragment includes a C-terminal fragment of a firefly luciferase split into two at a splitting position specific to the firefly luciferase, and 58 to 78 amino acid residues toward the N-terminal beyond the splitting position. When the N-terminal and C-terminal fragments are bound together, firefly luciferase activity is exhibited.
    Type: Application
    Filed: March 7, 2017
    Publication date: June 29, 2017
    Applicant: OLYMPUS CORPORATION
    Inventor: Takashi SUGIYAMA
  • Synthetic luciferases with enhanced light output
    Patent number: 9777311
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: October 14, 2013
    Date of Patent: October 3, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • Thermostable luciferases and methods of production
    Publication number: 20030068801
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lifes of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Application
    Filed: April 19, 2001
    Publication date: April 10, 2003
    Applicant: Promega Corporation
    Inventors: Keith V. Wood, Mary P. Hall
  • Synthetic oplophorus luciferases with enhanced light output
    Patent number: 10233485
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: September 25, 2017
    Date of Patent: March 19, 2019
    Assignee: PROMEGA CORPORATION
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT
    Publication number: 20100281552
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Application
    Filed: May 3, 2010
    Publication date: November 4, 2010
    Inventors: Lance P. Encell, Keith V. Wood, Monika G. Wood, Mary Hall, Paul Otto, Gediminas Vidugiris, Kristopher Zimmerman
  • Thermostable luciferases and methods of production
    Publication number: 20030232404
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Application
    Filed: February 28, 2003
    Publication date: December 18, 2003
    Applicant: Promega Corporation
    Inventors: Keith V. Wood, Mary P. Hall, Monika G. Wood
  • Synthetic oplophorus luciferases with enhanced light output
    Patent number: 10633690
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: April 28, 2020
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • Thermostable luciferases and methods of production
    Patent number: 6602677
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Grant
    Filed: September 15, 1999
    Date of Patent: August 5, 2003
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Mary P. Hall
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