Search Patents
  • Synthetic luciferases with enhanced light output
    Patent number: 11667950
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: June 20, 2022
    Date of Patent: June 6, 2023
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • Luciferase inhibitor compositions and methods of using same
    Patent number: 5814471
    Abstract: Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions utilizing a luciferase inhibitor. Thus, the invention provides methods and compositions for assaying samples for the presence of a beetle luciferase in conjunction with a luciferase inhibitor.
    Type: Grant
    Filed: April 9, 1997
    Date of Patent: September 29, 1998
    Assignee: Promega Corporation
    Inventor: Keith V. Wood
  • Synthetic oplophorus luciferases with enhanced light output
    Patent number: 11365436
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: October 20, 2020
    Date of Patent: June 21, 2022
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary Hall, Paul Otto, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kristopher Zimmerman
  • Luciferase assay compositions
    Patent number: 5650289
    Abstract: Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and luciferin (D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-.DELTA..sup.2 -thiazoline-4-carboxylic acid).
    Type: Grant
    Filed: January 31, 1994
    Date of Patent: July 22, 1997
    Assignee: Promega Corporation
    Inventor: Keith V. Wood
  • Thermostable luciferases and methods of production
    Patent number: 8030017
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Grant
    Filed: July 31, 2009
    Date of Patent: October 4, 2011
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Monika G. Wood, Mary P. Hall
  • Kit for luciferase assay
    Patent number: 5641641
    Abstract: Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and luciferin (D-(-)-2-(6'-hydroxy -2'-benzothiazolyl)-.DELTA..sup.2 -thiazoline-4-carboxylic acid).
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: June 24, 1997
    Assignee: Promega Corporation
    Inventor: Keith V. Wood
  • Thermostable luciferases and methods of production
    Patent number: 7241584
    Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
    Type: Grant
    Filed: February 28, 2003
    Date of Patent: July 10, 2007
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Mary P. Hall, Monika G. Wood
  • Synthetic luciferases with enhanced light output
    Patent number: 8557970
    Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: May 3, 2010
    Date of Patent: October 15, 2013
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Keith V. Wood, Monika G. Wood, Mary Hall, Paul Otto, Gediminas Vidugiris, Kristopher Zimmerman
  • Modified luciferase
    Publication number: 20050079567
    Abstract: The invention comprises modified luciferase proteins which are more resistant to inhibition by test chemicals than wild type luciferase. The modified luciferases also contain greater thermostability than wild type luciferase.
    Type: Application
    Filed: September 30, 2004
    Publication date: April 14, 2005
    Applicant: Board of Control of Michigan Technological University
    Inventors: Eileen Kim Choi, David Shonnard, Irina Rybina
  • Mutant firefly luciferase, gene, recombinant vector, transformant, and method for production of mutant firefly luciferase
    Publication number: 20090305353
    Abstract: The present invention provides a mutant firefly luciferase consisting of a mutant amino acid sequence derived from the amino acid sequence of a wild-type firefly luciferase by at least substitution (a), (b), or (c) below and having luminescence intensity higher than that of the wild-type firefly luciferase.
    Type: Application
    Filed: September 6, 2006
    Publication date: December 10, 2009
    Inventors: Hiroya Fujii, Kenichi Noda
  • MODIFIED LUCIOLA CRUCIATA LUCIFERASE GENE AND PROTEIN
    Publication number: 20100093029
    Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
    Type: Application
    Filed: October 10, 2008
    Publication date: April 15, 2010
    Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
  • MODIFIED LUCIOLA CRUCIATA LUCIFERASE PROTEIN
    Publication number: 20120034634
    Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
    Type: Application
    Filed: May 24, 2010
    Publication date: February 9, 2012
    Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
  • Mutant firefly luciferase, gene, recombinant vector, transformant, and method for production of mutant firefly luciferase
    Patent number: 8003350
    Abstract: The present invention provides a mutant firefly luciferase consisting of a mutant amino acid sequence derived from the amino acid sequence of a wild-type firefly luciferase by at least substitution (a), (b), or (c) below and having luminescence intensity higher than that of the wild-type firefly luciferase.
    Type: Grant
    Filed: September 6, 2006
    Date of Patent: August 23, 2011
    Assignee: Bioenex Inc.
    Inventors: Hiroya Fujii, Kenichi Noda
  • Luciferases
    Patent number: 6132983
    Abstract: Proteins are provided having luciferase activity with greater heat stability than wildtype luciferases by replacing the glutamate equivalent to that at position 354 of Photinus pyralis luciferase or 356 of Luciola luciferases with an alternative amino acid, particularly lysine. DNA, vectors and cells that encode for and express the proteins are also provided as are test kits and reagents for carrying out luminescence assays using the proteins of the invention. Preferred proteins have a second replaced amino acid at a position equivalent to position 215 of Photinus pyralis luciferase or 217 of Luciola luciferases.
    Type: Grant
    Filed: November 20, 1996
    Date of Patent: October 17, 2000
    Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland
    Inventors: Christopher Robin Lowe, Peter John White, James Augusts Henry Murray, David James Squirrell
  • GENE ENCODING NOVEL LUCIFERASE
    Publication number: 20090233320
    Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.
    Type: Application
    Filed: December 9, 2004
    Publication date: September 17, 2009
    Applicant: NEC Soft, Ltd.
    Inventor: Hiromi Takenaka
  • Two secretory luciferases
    Publication number: 20110223610
    Abstract: The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.
    Type: Application
    Filed: October 23, 2009
    Publication date: September 15, 2011
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • FIREFLY LUCIFERASE
    Publication number: 20150291938
    Abstract: The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.
    Type: Application
    Filed: April 11, 2014
    Publication date: October 15, 2015
    Applicants: OLYMPUS CORPORATION, PERAK STATE DEVELOPMENT CORPORATION, NIMURA GENETIC SOLUTIONS CO., LTD.
    Inventors: Ryutaro AKIYOSHI, Katsunori OGO, Hirobumi SUZUKI
  • Firefly luciferase
    Patent number: 9523082
    Abstract: The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: December 20, 2016
    Assignees: OLYMPUS CORPORATION, NIMURA GENETIC SOLUTIONS CO., LTD., PERAK STATE DEVELOPMENT CORPORATION
    Inventors: Ryutaro Akiyoshi, Katsunori Ogo, Hirobumi Suzuki
  • PERMUTED AND NONPERMUTED LUCIFERASE BIOSENSORS
    Publication number: 20160340654
    Abstract: A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.
    Type: Application
    Filed: May 31, 2016
    Publication date: November 24, 2016
    Inventors: Brock Binkowski, Frank Fan, Susan Wigdal, Keith V. Wood, Monika G. Wood
  • Modified luciferase
    Patent number: 7183092
    Abstract: The invention comprises modified luciferase proteins which are more resistant to inhibition by test chemicals than wild type luciferase. The modified luciferases also contain greater thermostability than wild type luciferase.
    Type: Grant
    Filed: September 30, 2004
    Date of Patent: February 27, 2007
    Assignee: Board of Control of Michigan Technological University
    Inventors: Eileen H. Kim Choi, David R. Shonnard, Irina V. Rybina
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