Search Patents
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Publication number: 20030232404Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.Type: ApplicationFiled: February 28, 2003Publication date: December 18, 2003Applicant: Promega CorporationInventors: Keith V. Wood, Mary P. Hall, Monika G. Wood
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Publication number: 20100093029Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.Type: ApplicationFiled: October 10, 2008Publication date: April 15, 2010Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
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Publication number: 20030166905Abstract: The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.Type: ApplicationFiled: June 28, 2002Publication date: September 4, 2003Applicant: Promega CorporationInventors: Keith V. Wood, Monika G. Wood
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Patent number: 8206961Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.Type: GrantFiled: May 24, 2010Date of Patent: June 26, 2012Assignee: Marker Gene Technologies, Inc.Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
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Patent number: 7723502Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.Type: GrantFiled: October 10, 2008Date of Patent: May 25, 2010Assignee: Marker Gene Technologies, IncInventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
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Publication number: 20040235077Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.Type: ApplicationFiled: April 22, 2004Publication date: November 25, 2004Applicant: Kikkoman CorporationInventors: Noriaki Hattori, Seiji Murakami
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Publication number: 20040002127Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.Type: ApplicationFiled: March 4, 2003Publication date: January 1, 2004Applicant: CHISSO CORPORATIONInventor: Satoshi Inouye
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Publication number: 20020119542Abstract: Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.Type: ApplicationFiled: November 14, 2001Publication date: August 29, 2002Inventors: Vadim R. Viviani, Yoshihiro Ohmiya
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Patent number: 7125697Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.Type: GrantFiled: March 4, 2003Date of Patent: October 24, 2006Assignee: Chisso CorporationInventor: Satoshi Inouye
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Patent number: 6232107Abstract: Isolated and purified nucleic acid molecules that encode a luciferase from Renilla mulleri, Gaussia and Pleuromamma, and the proteins encoded thereby are provided. Isolated and purified nucleic acids encoding green fluorescent proteins from the genus Renilla and Ptilosarcus, and the green fluorescent proteins encoded thereby are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.Type: GrantFiled: March 26, 1999Date of Patent: May 15, 2001Assignees: Prolume, LTDInventors: Bruce J. Bryan, Christopher Szent-Gyorgyi
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Publication number: 20090136998Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: ApplicationFiled: September 6, 2006Publication date: May 28, 2009Inventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Publication number: 20040219527Abstract: The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22.Type: ApplicationFiled: October 1, 2003Publication date: November 4, 2004Inventors: Stefan Golz, Bernd Kalthof, Svetlana Markova, Ludmila Frank, Eugene Vysotski
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Publication number: 20030119012Abstract: The present invention relates to the field of DNA recombinant technology. More specifically, this invention relates to fusion proteins comprising an ATP generating polypeptide joined to a polypeptide that converts ATP into a detectable entity. Accordingly, this invention focuses on sulfurylase-luciferase fusion proteins. This invention also relates to pharmaceutical compositions containing the fusion proteins and methods for using them.Type: ApplicationFiled: April 11, 2002Publication date: June 26, 2003Inventors: Maithreyan Srinivasan, Michael Reifler
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Publication number: 20030113747Abstract: The present invention relates to the field of DNA recombinant technology. More specifically, this invention relates to fusion proteins comprising an ATP generating polypeptide joined to a polypeptide that converts ATP into a detectable entity. Accordingly, this invention focuses on sulfurylase-luciferase fusion proteins. This invention also relates to pharmaceutical compositions containing the fusion proteins and methods for using them.Type: ApplicationFiled: May 23, 2002Publication date: June 19, 2003Inventors: Maithreyan Srinivasan, Michael Reifler
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Patent number: 7666616Abstract: The present invention relates to genetic reporters. Specifically, the present invention is directed to a modified gene encoding a luciferase for high level expression in an organism with a bias for cytosine (C) or guanine (G) in the third position of the codon.Type: GrantFiled: September 30, 2005Date of Patent: February 23, 2010Assignees: Trustees of Dartmouth College, Regents of the University of MinnesotaInventors: Jay C. Dunlap, Jennifer Loros, Arun Mehra, Van D. Gooch
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Patent number: 11913033Abstract: The present invention is directed to nucleic acid molecules which encode novel luciferases, functional fragments thereof, homologs and mutants, as well as to proteins encoded by said nucleic acids. The nucleic acid molecules of interest are isolated from fungi or obtained by genetic engineering methods. Also, host cells, stable cell lines and transgenic organisms comprising said nucleic acid molecules are provided. In addition, antibodies specific to the proteins of the present invention are provided. Said proteins and nucleic acids are used in many applications and methods, in particular, in labelling organisms, cells, cellular organelles, or proteins. Also, said protein and nucleotide compositions are used in methods for detecting protein-protein interactions, for testing promoter activity under various conditions. Finally, provided are kits for the use of proteins and nucleic acids of the present invention in the diversity of methods and applications.Type: GrantFiled: December 6, 2017Date of Patent: February 27, 2024Assignee: Light Bio, Inc.Inventor: Il'ya Viktorovich Yampol'skiy
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Publication number: 20030157519Abstract: The invention relates to compositions comprising a first fusion protein comprising a first polypeptide domain and a R. reniformis luciferase and a second fusion protein comprising a second polypeptide domain and a R. reniformis GFP. The invention also relates to compositions comprising one or more polynucleotides encoding a first fusion protein comprising a first polypeptide domain and a R. reniformis luciferase and a second fusion protein comprising a second polypeptide domain and a R. reniformis GFP. The invention also relates to methods and kits for detecting protein-protein interactions, determining the location of a protein-protein interaction, identifying cells wherein there is a protein-protein interaction of interest, and screening for a candidate modulator that increases or decreases the amount of a protein-protein interaction.Type: ApplicationFiled: October 4, 2002Publication date: August 21, 2003Applicant: StratageneInventors: Vivan Q. Zhang, Peter E. Vaillancourt
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Patent number: 7517647Abstract: A method of screening for a protein involved in the extracellular regulation of latent TGF? activation by transducing a cell line with a retroviral cDNA library to create a reporter cell line that produces green fluorescent protein (GFP) in response to TGF-? signaling; growing individual clones created by the reporter cell line; co-culturing each individual clone with a second TGF-? reporter cell line that produces luciferase in response to TGF-?, wherein the luciferase production identifies positive clones; and identifying a mechanism of latent TGF-? activation that is employed by the positive clones. A TGF? reporter cell line including a cell line; and a retroviral cDNA library, wherein the reporter cell line produces GFP in response to TGF-? signaling. A method of screening for gene products involved in a biological process.Type: GrantFiled: September 29, 2004Date of Patent: April 14, 2009Assignee: New York UniversityInventors: Daniel B. Rifkin, John S. Munger, Justin P. Annes
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Publication number: 20030092098Abstract: Isolated and purified nucleic acids encoding green fluorescent proteins from Renilla reniformis and the green fluorescent protein encoded thereby are also provided. Mutants of the nucleic acid molecules and the modified encoded proteins are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.Type: ApplicationFiled: March 15, 2001Publication date: May 15, 2003Inventors: Bruce Bryan, Christopher Szent-Gyorgyi, William Szczepaniak
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Patent number: 7109315Abstract: Isolated and purified nucleic acids encoding green fluorescent proteins from Renilla reniformis and the green fluorescent protein encoded thereby are also provided. Mutants of the nucleic acid molecules and the modified encoded proteins are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.Type: GrantFiled: March 15, 2001Date of Patent: September 19, 2006Assignees: Prolone, Ltd.Inventors: Bruce Bryan, Christopher Szent-Gyorgyi, William Szczepaniak