Abstract: The present invention relates to the modification of hyaluronic acid (HA) with aryl/alkyl succinic anhydrides (ASA) to produce aryl/alkyl succinic anhydride HA derivatives, to the derivatives as such, and to their applications and uses, particularly in the cosmetic and biomedical industries. The ASA-HA derivatives are expected to have interesting properties that can be used for advanced formulation (bind stronger to the skin compared to non-modified HA), possibly also in delivery systems for actives or drugs by encapsulation (nano/micro capsules) or formation of nano/micro spheres. Further, the low MW ASA-HA derivatives are expected to penetrate the skin more efficiently than non-modified HA of the same MW.
Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+dependency and specific activity.
Type:
Grant
Filed:
September 27, 2007
Date of Patent:
June 14, 2011
Assignee:
Novozymes A/S
Inventors:
Allan Svendsen, Torben Vedel Borchert, Henrik Bisgaard-Frantzen
Abstract: The present invention relates to products comprising hyaluronic acid or a salt thereof, which product is dried and agglomerated as defined herein, various compositions and articles comprising the product or compositions of the invention, methods of producing the product of the invention, and uses thereof.
Abstract: The present invention relates to a method comprising treatment with cutinase for detoxification of feed products contaminated by the mycotoxin zearalenone.
Type:
Grant
Filed:
December 19, 2008
Date of Patent:
May 17, 2011
Assignee:
Novozymes A/S
Inventors:
Anders Viksoe-Nielsen, Birthe Hauerbach Soerensen
Abstract: The invention relates to the use of co-expression of an antimicrobial agent and an enzyme, with a view to improving yield and/or overall production economy. Examples of antimicrobial agents are antimicrobial peptides such as lactoferricins and antimicrobial enzymes such as lysozyme and glucose oxidase, and examples of enzymes are endoglucanase, xylanase, phytase, protease, galactanase, mannanase, dextranase, alpha-galactosidase, pectate lyase, alpha-amylase and glucoamylase. A fusion product comprising the antimicrobial agent and the enzyme and a cleavable linker is novel, and can be used in animal feed and animal feed additives. The invention also describes the use of a protection domain wherein at least 50% of the amino acid residues comprised in the peptide protection domain are D (Asp) and/or E (Glu). The protection or quenching domain serves to temporarily and reversibly inactivate the antimicrobial peptide during the expression.
Type:
Grant
Filed:
September 13, 2004
Date of Patent:
May 3, 2011
Assignee:
Novozymes Pharma Biotec A/S
Inventors:
Ejner Bech Jensen, Hans-Henrik Kristensen Høgenhaug, Peter Kamp Hansen, Poul Erik Pedersen, Per Holse Mygind
Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.
Type:
Grant
Filed:
February 21, 2008
Date of Patent:
April 19, 2011
Assignee:
Novozymes A/S
Inventors:
Bjarne Ronfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
Abstract: The present invention relates to isolated polypeptides having phytase activity and isolated polynucleotides encoding the polypeptides. The polypeptides are related to a phytase derived from Hafnia alvei, the amino acid sequence of which is shown in the appended sequence listing as SEQ ID NO: 10. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides, in particular within animal feed.
Type:
Grant
Filed:
March 26, 2008
Date of Patent:
April 12, 2011
Assignee:
Novozymes A/S
Inventors:
Soeren Flensted Lassen, Carsten Sjoeholm, Lars Kobberoee Skov
Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.
Abstract: The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostability, altered substrate specificity, increased specific activity or altered cleavage pattern.
Abstract: The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g., in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
Type:
Grant
Filed:
May 15, 2009
Date of Patent:
March 15, 2011
Assignee:
Novozymes A/S
Inventors:
Peter Rahbek Oestergaard, Leonardo De Maria
Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.
Type:
Grant
Filed:
June 25, 2004
Date of Patent:
February 8, 2011
Assignees:
Novozymes A/S, Novozymes North America, Inc.
Inventors:
Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Carsten Hjort, Anders Vikso-Nielsen, Eric Allain, Shiro Fukuyama, Tomoko Matsui
Abstract: The invention relates to a phytase derived from Citrobacter braakii and related phytases. The phytases belong to the acid histidine phosphatase family, are acid-stable, of an excellent performance in animal feed, of a high specificity towards the substrate phytate, and expectedly of a high specific activity. The invention also relates to the corresponding DNA, the recombinant and wild-type production of the phytases, as well as the use thereof.
Type:
Grant
Filed:
October 4, 2005
Date of Patent:
January 11, 2011
Assignee:
Novozymes A/S
Inventors:
Monica Takamiya Wik, Carsten Sjøholm, Henrik Frisner, Allan Noergaard, Mikael Blom Sørensen
Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
Type:
Grant
Filed:
November 2, 2007
Date of Patent:
January 11, 2011
Assignee:
Novozymes A/S
Inventors:
Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgard-Frantzen, Allan Svendsen, Soren Kjaerulff
Abstract: The invention relates to Citrobacter phytases derived from Citrobacter amalonaticus, Citrobacter gillenii, and related phytases. The phytases belong to the acid histidine phosphatase family, are acid-stable, and expectedly of a high specific activity. The invention also relates to the corresponding DNA, the recombinant and wild-type production of the phytases, as well as the use thereof, in particular in animal feed.
Abstract: The present invention relates to a process for producing a fermentation product from starch-containing material, comprising liquefying said starch-containing material with an alpha-amylase; treating with a protease; saccharifying in the presence of a carbohydrate-source generating enzyme; fermenting in the presence of a fermenting organism.
Type:
Grant
Filed:
February 7, 2006
Date of Patent:
October 26, 2010
Assignee:
Novozymes North America Inc.
Inventors:
Mads Torry Smith, John Ress, Kevin S. Wenger, Rikke Monica Festersen
Abstract: The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isoamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.
Type:
Grant
Filed:
May 5, 2008
Date of Patent:
October 19, 2010
Assignee:
Novozymes Als
Inventors:
Henrik Bisgård-Frantzen, Allan Svendsen
Abstract: The present invention relates to a hybrid enzyme comprising at least one carbohydrate-binding module amino acid sequence and at least the catalytic module of a glucoamylase amino acid sequence. The invention also relates to the use of the hybrid enzyme in starch processing and especially ethanol production.
Type:
Grant
Filed:
December 11, 2007
Date of Patent:
July 6, 2010
Assignees:
Novozymes A/S, Novozymes North America, Inc.
Inventors:
Torben V. Borchert, Steffen Danielsen, Eric J. Allain
Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.
Type:
Grant
Filed:
December 22, 2009
Date of Patent:
May 11, 2010
Assignee:
Novozymes A/S
Inventors:
Thomas Thisted, Soren Kjaerulff, Carsten Andersen, Claus Crone Fuglsang
Abstract: The present invention relates to isolated polypeptides having antimicrobial activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Abstract: The present invention relates to novel subtilases from wild-type strains of Bacillus, especially the Bacillus strains ZI344, EP655, P203, EP63, ZI120, ZI130, ZI1342 and ZI140, and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry detergent or an automatic dishwashing detergent.