Abstract: The present invention provides a process for removal of excess dye from newly manufactured printed or dyed fabric or yarn, comprising treatment with a rinse liquor comprising at least one enzyme selected from the group consisting of enzymes exhibiting peroxidase activity or laccase activity, an oxidation agent, and at least one mediator comprising N-hydroxyacetanilide.

Abstract: A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15% to more than 80%, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.

Abstract: Disclosed is a process for preparing a dough or a baked product from the dough which process involves incorporating into the dough an anti-staling amylase and a phospholipase. The bread made by the combined use of an anti-staling amylase and a phospholipase has improved softness, both when eaten on the same day and when stored for several days after baking. There is no significant change in the taste or smell of the baked product.

Abstract: A substantially water-free, liquid, enzyme-containing composition comprises: (A) an enzyme; (B) a substance selected from (i) substances which in aqueous medium are substrates for said enzyme, (ii) substances which in aqueous medium are precursors for substrates for said enzyme, and (iii) substances which are cofactors for said enzyme; and (C) a non-aqueous liquid phase.

Abstract: Lipolytic enzymes with high activity at alkaline pH in the absence of Ca++ can be obtained from filamentous fungi of the genera Gliocladium, Verticillium and Trichophaea and that the lipolytic enzymes are effective for improving the effect of detergents. The lipolytic enzymes have a good washing performance, as expressed by the hydrolysis of oil on textile swatches. The amino acid sequences of the lipolytic enzymes are highly homologous.

Abstract: This invention relates to an enzyme containing granular composition comprising: a) an enzyme containing core and b) a protective substantially continuous layer or coating encapsulating the core comprising at least 60% of a water soluble compound, having a molecular weight below 500 grams per mole, a pH below 11 and a constant humidity at 20° C. of more than 81%. The invention provides an improved stability of enzymes upon storage.

Abstract: The present invention deals with a process for oxidation of a hydroxy group of C1 and/or C2 and/or C3 and/or C4 and/or C5 and/or C6 of a sugar monomer of an oligo- or a polysaccharide comprising contacting, in an aqueous medium, the oligo- or the polysaccharide with a phenol oxidizing enzyme and an enhancing agent, whereby an oligo- or a polysaccharide with altered characteristics compared to the native oligo- or polysaccharide is created.

Abstract: The present invention relates to a method of producing oligosaccharide syrups, in particular to the production of syrups having a high concentration of saccharides with a degree of polymerization of at least 2, comprising the steps of: enzymatic reaction of a substrate at a temperature in the range of 50° C. to 100° C. obtaining a saccharide solution comprising monosaccharides and disaccharides, trisaccharides and higher saccharides; nanofiltration of the saccharide solution at a temperature in the range of 60° C. to 100° C. obtaining a syrup essentially comprising disaccharides, trisaccharides and higher saccharides; recovering said syrup; optionally recycling the permeate resulting from the nanofiltration step to the enzymatic reaction.

Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability.

Abstract: The present invention relates to a method for in vivo recombination of homologous DNA sequences. The method is a forced artificial evolution resulting in a DNA sequence encoding a polypeptide having an advantageous property.

Abstract: A method has been found for reducing amino acid biosynthesis inhibiting effect of a sulfonyl-urea based compound of the general formula: comprising contacting in an aqueous solution said sulfonyl-urea based compound with an enzyme capable of reducing said amino acid biosynthesis inhibiting effect.

Abstract: The present invention relates to isolated polypeptides having polypeptide having 2,6-&bgr;-D-fructan hydrolase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Abstract: The present invention relates to a method for recovering a glycosidase or a peptidase from a culture solution from an unsolubilized state within said solution to a solubilized state within said solution.

Abstract: The present invention relates to isolated polypeptides having &agr;-amylase activity and isolated nucleic acid sequences encoding the polypeptides, which may be derived from Bacillus. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Abstract: Lipases having improved washing performance for use in detergents to improve the removal of fatty soiling, and having activity throughout a temperature range of 1 to 65° C., including an activity at 1° C. which is at least 20% the activity at 30° C., activity over a pH range of 8-12, and activity at high concentration of anionic surfactant and in the presence of bleach.

Abstract: The present invention relates to a method for the production of saccharide preparations, i.e., syrups, by saccharifying a liquefied starch solution, which method comprises a saccharification step during which step one or more enzymatic saccharification stages takes place, and the subsequent steps of one or more high temperature membrane separation steps, and re-circulation of the saccharification enzyme, in which method the membrane separation steps are carried out as an integral part of the saccharification step. In another specific aspect, the invention provides a method of producing a saccharide preparation, which method comprises an enzymatic saccharification step, and the subsequent steps of one or more high temperature membrane separation steps and re-circulation of the saccharification enzyme.

Abstract: A process for preparing tobacco, which process comprises the steps of treating a tobacco material with a phenol oxidising enzyme, such as by extracting tobacco with a solvent to provide an extract and a residue; and treating the extract with a phenol oxidising enzyme such as a laccase. An improved tobacco product having a reduced amount of phenolic compounds. This is an alternative or a supplement to a process in which the phenolic compounds are adsorbed onto the insoluble carrier polyvinylpolypyrrolidone (PVPP). In preferred embodiments, the process includes further steps of removing the oxidised phenolic compound, adding adsorbents such as bentonite; removing and/or inactivating the enzyme; and concentrating the extract. Preferred phenol oxidising enzymes are peroxidases and laccases. The thus treated extract is advantageously re-combined with the tobacco residue and further processed to provide a tobacco article for smoking.

Abstract: A dough- or bread-improving composition comprising laccase as well as the use of the composition in the preparation of dough and baked products.

Abstract: The present invention relates to variants of a parent &agr;-amylase, which parent &agr;-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.

Abstract: The present invention relates to a DNA construct exhibiting &bgr;-1,3-glucanase activity, which DNA sequence comprises: a) the DNA sequence shown in SEQ ID No. 1 or SEQ ID No. 12, or b) an analogue of the DNA sequence defined in a), which i) is homologous with the DNA sequence defined in a), or ii) hybridizes with the same oligonucleotide probe as the DNA sequence defined in a), or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence defined in a), or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against the purified &bgr;-1,3-glucanase derived from Oerksovia xanthineolytica LLG109 encoded by the DNA sequence defined in a). The DNA construct may further encode a mannose binding domain. The invention also relates to a DNA construct encoding a mannose binding domain.