Abstract: Specific exchange of the acyl group in the sn-2 position of a phospholipid is achieved by reacting it with a free fatty acid in the presence of an extracellular phospholipase A2.
Abstract: The present invention relates to a method of isolating proteins from a proteinaceous vegetable material. More specifically the invention provides a method for isolating proteins from a proteinaceous vegetable material, which method involves the steps of subjecting the proteinaceous vegetable material to the action of one or more carbohydrate degrading enzyme(s), thereby obtaining a mixture comprising proteins and hydrolyzed carbohydrates; and subjecting the mixture of step (i) to a separation process in order to separate the proteins from the hydrolyzed carbohydrates.
Abstract: An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.
Type:
Grant
Filed:
January 7, 1999
Date of Patent:
August 28, 2001
Assignee:
Novozymes A/S
Inventors:
Helle Outtrup, Claus Dambmann, Arne Agerlin Olsen, Henrik Bisgård-Frantzen, Martin Schülein, Per Linaa Jorgensen, Mads E. Bjoernvad
Abstract: The present invention relates to a method of designing laccase mutants with improved stability properties, which method is based on the hitherto unknown three-dimensional structure of Coprinus cinereus laccase.
Type:
Grant
Filed:
May 23, 2000
Date of Patent:
August 21, 2001
Assignee:
Novozymeo A/S
Inventors:
Anders Hjelholt Pedersen, Allan Svendsen, Palle Schneider, Grethe Rasmussen, Joel Cherry
Abstract: A method for providing a hybrid polypeptide having an activity of interest, by i) performing PCR amplification using an uncharacterized DNA sample and oligonucleotide primers with homology to one or more known genes encoding a polypeptide exhibiting said activity of interest, to obtain one or more PCR products, ii) linking the obtained PCR products to a 5′ structural gene sequence and a 3′ structural gene sequence, wherein the 5′ and 3′ structural gene sequences are derived from one or more genes encoding a polypeptide exhibiting said activity of interest, to form hybrid DNA sequences, iii) expressing the resulting hybrid DNA sequences, and iv) screening the hybrid polypeptides to identify a sequence encoding a polypeptide exhibiting said activity of interest or a related activity.
Type:
Grant
Filed:
November 5, 1998
Date of Patent:
August 7, 2001
Assignee:
Novozymes A/S
Inventors:
Henrik Dalbøge, Thomas Sandal, Markus Sakari Kauppinen, Børge Diderichsen
Abstract: A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.
Type:
Grant
Filed:
July 7, 1998
Date of Patent:
July 31, 2001
Assignee:
Novozymes A/S
Inventors:
Martin Schulein, Helle Outtrup, Per Lina Jorgensen, Mads Eskelund Bjornvad
Abstract: This invention relates to an enzyme containing granular composition comprising:
a) an enzyme containing core and
b) a protective substantially continuous layer or coating encapsulating the core comprising at least 60% of a water soluble compound, having a molecular weight below 500 grams per mole, a pH below 11 and a constant humidity at 20° C. of more than 81%.
The invention provides an improved stability of enzymes upon storage.
Abstract: Novel yeast promoters for either EF1-alpha protein or ribosomal protein S7 gene suitable for expression cloning in yeast and heterologous expression of proteins in yeast. The yeast promoters are preferably active in the pH range 4-11 without peptone and obtained from the yeast strain Yarrowia lipolytica.
Abstract: The present invention relates to methods of producing wool or animal hair material with improved properties such as shrink-proofed (anti-felting tendency), increased whiteness, improved dyeability, increased softness and/or reduced pilling tendency, the method comprising the steps of treating wool, wool fibers or animal hair material in a process selected from the group consisting of plasma treatment processes and the Delhey process, and subjecting the wool or animal hair material to a treatment with a proteolytic enzyme (a protease), preferably a serine protease, more preferably a subtilisin, in an amount effective for improving the properties.
Type:
Grant
Filed:
June 6, 1997
Date of Patent:
July 10, 2001
Assignee:
Novozymes A/S
Inventors:
Lone Dybdal, Elisabeth Heine, Hartwig Hocker
Abstract: The present invention provides methods for higha-temperature biopreparation of cellulosic fibers by contacting the fibers with pectin-degrading enzymes, preferably thermostable, alkaline, divalent cation-independent pectate lyases, under conditions compatible with scouring and bleaching technologies.
Type:
Grant
Filed:
November 2, 1998
Date of Patent:
July 10, 2001
Assignees:
Novozymes A/S, Novozymes North America
Inventors:
Niels Erik Krebs Lange, Lars Kongsbak, Martin Shülein, Mads Eskelund Bjørnvad, Philip Anwar Husain
Abstract: An enzymatic antimicrobial composition comprising a haloperoxidase, a hydrogen peroxide source, a halide source, and an ammonium source, in particular an ammonium salt or an aminoalcohol, in which there is a hitherto unknown synergistic effect between the halide and the ammonium source.
Abstract: A method for causing gelling or increase of viscosity of an aqueous medium containing a gellable polymeric material having substituents with phenolic hydroxy groups comprises adding an oxidase, particularly a laccase, to the aqueous medium.
Type:
Grant
Filed:
October 28, 1996
Date of Patent:
May 15, 2001
Assignee:
Novozymes A/S Patents
Inventors:
Gitte Budolfsen, Hans Peter Heldt-Hansen