Abstract: Disclosed is a process whereby enzymes are immobilized on granular diatomaceous earth. The process involves treating the diatomaceous earth with a polyamine compound having pendant amino groups to cause the polyamine to adhere to the diatomaceous earth leaving pendant amine groups free to react further. The free amine groups are derivatized by treatment with a difunctional compound having amine reactive moieties, so that free amine groups of an enzyme or enzymes can be covalently bound to the polyamine via the amine reactive compound.
Abstract: This invention relates to a novel process for the recovery of enzymes obtained from enzyme-producing microorganisms, and to the liquid enzyme product recovered by this process. Typically, the enzyme-containing filtrate from a fermentation of an enzyme-secreting microorganism is concentrated and a precipitation agent such as a salt or an organic solvent is added to the concentrate, thereby forming a cake. Then, a polyol solvent is circulated through the cake to solubilize the enzyme or enzyme complex from the cake and provide a liquid enzyme product. Particularly effective is propylene glycol as the polyol solvent. The liquid enzyme product may be shipped as is or subjected to further treatment to remove the solvent and create an essentially solvent-free enzyme product. The process is especially effective for the recovery of alkaline protease or alpa amylase.
Abstract: Described is a directly compressible formulation comprising calcium carbonate, citric acid, and a compression vehicle. In the preferred embodiment, the compression vehicle comprises a maltodextrin and lactose. The formulation can be compressed into an effervescent tablet system, which when placed in water effervesces in a relatively rapid dissolution releasing CO.sub.2 and resulting in a solution of monocalcium citrate.
Type:
Grant
Filed:
July 30, 1985
Date of Patent:
March 17, 1987
Assignee:
Miles Laboratories, Inc.
Inventors:
Thomas A. Alexander, Donald L. Peterson
Abstract: The invention is directed to an improvement in flexible side-sealed taped bags having an extended lip rear panel. The external surface of the rear panel is releasably secured, at least at one point, to at least one tape (preferably two tapes) whereby a plurality of the bags may be advantageously presented in imbricated form. The improvement includes extension of the side seals of the front bag panel to the rear bag panel. The length of the extended side seals being such that their termination points are extended beyond the bag mouth so that the forces imposed on the bag mouth during loading of a product into the bag, while the bag is secured to the tape(s), act on the side seals below the side seal termination points. During product loading, the bag preferentially releases or separates from the tape(s) rather than tearing or delaminating at the side seal termination points.
Type:
Grant
Filed:
September 18, 1984
Date of Patent:
January 6, 1987
Assignee:
W. R. Grace & Co., Cryovac Div.
Inventors:
Robert A. Odabashian, Benjamin G. Wofford
Abstract: Spherically shaped bacterial cell aggregates are produced by spheronizing extruded flocculated cells. The cells are flocculated from aqueous medium with a cross-linked polyamine which is the reaction product of an epihalohydrin/polyamine copolymer and a cross-linking agent. Prior to being extruded, a filter cake having 68 to 76 weight percent water is produced by filtration of the flocculated cells, and the filter cake is ground into particles no greater than 60 mesh. Spheronizing is with a plate rotating at a tangential velocity of 4.5 to 12 meters per second within a cylinder containing the plate. Toughness of the spherical aggregates produced can be increased by the addition of a binder after filtration and before extrusion. During spheronizing, fines may be produced. These fines can be recycled by mixing them with the wet filter cake and binder before extrusion.
Abstract: The invention relates to methods of controlling the precipitation or depositing of particles from a solution in a cell in which an electric field is applied between an anode and a cathode, in particular the precipitation of metal compounds such as oxides and hydroxides and the coprecipitation of mixed metal oxides from a solution of the metal salt(s) in an electrolysis cell, but also the electrolytic deposition of cathodic metal deposits as well as the electrophoretic deposition of colloidal particles.
Type:
Grant
Filed:
June 13, 1983
Date of Patent:
October 2, 1984
Inventors:
Henri Beer, Franz A. M. Van Den Keybus, Ludovicus F. M. Suyberbuyk
Abstract: Diaphragm coated electrodes, processes for producing these diaphragm coated electrodes, and application of the diaphragm coated electrodes in electrolytic cells used in the electrolysis of brine to produce chlorine and caustic are disclosed.
Type:
Grant
Filed:
March 29, 1982
Date of Patent:
April 24, 1984
Assignee:
Diamond Shamrock Corporation
Inventors:
Shan-Pu Tsai, Leo L. Benezra, Lewis R. Horvat, John P. Hazzard