Abstract: The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.
Type:
Grant
Filed:
February 26, 2020
Date of Patent:
October 12, 2021
Assignee:
Genomatica, Inc.
Inventors:
Derek L. Greenfield, Donald E. Trimbur, Andreas W. Schirmer, Cindy Chang, Behnaz Behrouzian, Jessica Winger
Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
Type:
Grant
Filed:
February 11, 2019
Date of Patent:
September 28, 2021
Assignee:
Genomatica, Inc.
Inventors:
Mathew Rude, Na Trinh, Andreas Schirmer, Jacob Gano
Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.
Type:
Grant
Filed:
May 2, 2017
Date of Patent:
August 10, 2021
Assignee:
Genomatica, Inc.
Inventors:
Priti Pharkya, Anthony P. Burgard, Stephen J. Van Dien, Robin E. Osterhout, Mark J. Burk, John D. Trawick, Michael P. Kuchinskas, Brian Steer
Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
Type:
Application
Filed:
August 24, 2020
Publication date:
July 29, 2021
Applicant:
Genomatica, Inc.
Inventors:
Andreas Schirmer, Baolong ZHU, Emanuela Popova
Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.
Type:
Grant
Filed:
April 2, 2012
Date of Patent:
July 20, 2021
Assignee:
Genomatica, Inc.
Inventors:
Derek L. Greenfield, Louis G. Hom, Fernando A. Sanchez-Riera, Zhihao Hu, Vikranth Arlagadda, Eli S. Groban, Scott A. Frykman
Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.
Type:
Grant
Filed:
July 14, 2017
Date of Patent:
July 13, 2021
Assignee:
Genomatica, Inc.
Inventors:
Derek L. Greenfield, Andreas W. Schirmer, Elizabeth J. Clarke, Eli S. Groban, Bernardo M. Da Costa, Zhihao Hu, Kevin Holden, Noah Helman
Abstract: The disclosure relates to engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives including e.g., eight carbon and ten carbon fatty acids and fatty acid derivatives. The disclosure further relates to recombinant host cells comprising the engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives. The disclosure also relates to methods of decreasing toxicity and improving production of medium-chain fatty acids and derivatives.
Type:
Application
Filed:
April 3, 2018
Publication date:
July 8, 2021
Applicant:
Genomatica, Inc.
Inventors:
Angelica Zabala BAUTISTA, Min Sun PARK, Alma Itzel RAMOS-SOLIS, Bernardo Moura TORRES DA COSTA, Behnaz BEHROUZIAN, Donald E. TRIMBUR, Emanuela POPOVA, Qijun HU, Stephen Bralley DEL CARDAYRE
Abstract: The disclosure relates to downstream processing of fatty alcohol (FALC) and provides a novel purification method that provides FALC at high purity and yield.
Type:
Grant
Filed:
March 7, 2014
Date of Patent:
July 6, 2021
Assignee:
GENOMATICA, INC.
Inventors:
Myong K. Ko, Haibo Wang, Patricia J. Cole, Perry Y. Liao, Simon Li
Abstract: Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.
Abstract: The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides novel multifunctional fatty acid derivative molecules such as e.g., fatty triols, fatty tetrols, dihydroxy fatty acids, etc. The disclosure further provides derivatives of the disclosed multifunctional molecules which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc. The disclosure further provides biochemical pathways, recombinant microorganisms and methods for the biological production of various multifunctional fatty acid derivatives.
Type:
Application
Filed:
May 3, 2019
Publication date:
June 24, 2021
Applicant:
Genomatica, Inc.
Inventors:
Andreas W. SCHIRMER, Risha Lindig BOND, Erin Frances PERRY
Abstract: The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.
Type:
Grant
Filed:
May 16, 2019
Date of Patent:
June 15, 2021
Assignee:
Genomatica, Inc.
Inventors:
Derek L. Greenfield, Elizabeth J. Clarke, Eli S. Groban, Vikranth Arlagadda, Sungwon Lee, Xuezhi Li, Zhihao Hu, Baolong Zhu
Abstract: The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.
Type:
Grant
Filed:
April 20, 2018
Date of Patent:
June 1, 2021
Assignee:
Genomatica, Inc.
Inventors:
Louis Hom, Na Trinh, Murtaza Alibhai, Zhihao Hu, Eli Groban, Vikranth Arlagadda, Elizabeth Clarke
Abstract: The present disclosure relates to processes that combine microbial production of organic intermediates and subsequent synthetic transformation to provide compounds of industrial value, including compounds used in fragrances.
Type:
Grant
Filed:
April 10, 2015
Date of Patent:
May 18, 2021
Assignee:
Genomatica, Inc.
Inventors:
Stephen B. Del Cardayre, Andreas W. Schirmer, Myong Ko, Haibo Wang
Abstract: The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source.
Type:
Grant
Filed:
August 22, 2019
Date of Patent:
April 13, 2021
Assignee:
GENOMATICA, INC.
Inventors:
Jason J. Lutes, Stephen B. Del Cardayre
Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.
Type:
Application
Filed:
June 29, 2020
Publication date:
March 11, 2021
Applicant:
Genomatica, Inc.
Inventors:
Andreas W. Schirmer, Haibo Wang, Stephen B. Del Cardayre, Zhihao Hu, Louis G. Hom, Baolong Zhu, Cindy Chang, Emanuela E. Popova
Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.
Type:
Application
Filed:
June 1, 2020
Publication date:
March 11, 2021
Applicant:
Genomatica, Inc.
Inventors:
Andreas W. Schirmer, Noah HELMAN, Haibo Wang, Zhihao Hu, Vikranth Arlagadda, Alma Itzel RAMOS-SOLIS, Elizabeth Clarke
Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.
Type:
Grant
Filed:
May 26, 2016
Date of Patent:
March 9, 2021
Assignees:
GENOMATICA, INC., BRASKEM S.A.
Inventors:
Stephanie J. Culler, Robert J. Haselbeck, Harish Nagarajan, Iuri Estrada Gouvea, Daniel Johannes Koch, Mateus Schreiner Garcez Lopes, Lucas Pedersen Parizzi
Abstract: Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.
Type:
Application
Filed:
January 7, 2020
Publication date:
February 11, 2021
Applicant:
Genomatica, Inc.
Inventors:
Andreas W. Schirmer, Mathew A. Rude, Shane A. BRUBAKER