Abstract: The disclosure relates to recombinant microorganisms for the production of fatty amines and derivatives thereof. Further contemplated are cultured recombinant host cells as well as methods of producing fatty amines by employing these host cells.
Abstract: The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.
Type:
Grant
Filed:
January 18, 2018
Date of Patent:
January 19, 2021
Assignee:
Genomatica, Inc.
Inventors:
Andrew M. Shumaker, Bernardo M. da Costa, Kevin Holden, Louis G. Hom, Tarah S. Baron, Noah Helman
Abstract: The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.
Type:
Application
Filed:
February 26, 2020
Publication date:
January 14, 2021
Applicant:
Genomatica, Inc.
Inventors:
Derek L. GREENFIELD, Donald E. TRIMBUR, Andreas W. SCHIRMER, Cindy CHANG, Behnaz BEHROUZIAN, Jessica WINGER
Abstract: The disclosure relates to the field of specialty chemicals. In particular, the disclosure provides novel 1,3-fatty-diol compounds and derivatives thereof which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc.
Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
Type:
Application
Filed:
October 23, 2019
Publication date:
November 26, 2020
Applicant:
Genomatica, Inc.
Inventors:
Baolong ZHU, Andreas W. Schirmer, Cindy CHANG
Abstract: Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.
Type:
Grant
Filed:
April 7, 2016
Date of Patent:
November 24, 2020
Assignee:
Genomatica Inc.
Inventors:
Stephanie J. Culler, Nicholas Eakley, Kevin Gregory Hoff, Robin E. Osterhout, Priti Pharkya, Erik C. Ralph, Stephen Van Dien
Abstract: Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.
Type:
Grant
Filed:
March 7, 2017
Date of Patent:
November 24, 2020
Assignee:
Genomatica, Inc.
Inventors:
Jay D. Keasling, Zhihao Hu, Chris Somerville, George Church, David Berry, Lisa C. Friedman, Andreas Schirmer, Shane Brubaker, Stephen B. Del Cardayre
Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention.
Type:
Grant
Filed:
November 25, 2014
Date of Patent:
October 20, 2020
Assignee:
Genomatica, Inc.
Inventors:
Robin E. Osterhout, Anthony P. Burgard, Priti Pharkya, Stefan Andrae
Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.
Type:
Grant
Filed:
December 19, 2016
Date of Patent:
October 6, 2020
Assignee:
Genomatica, Inc.
Inventors:
Robin E. Osterhout, Anthony P. Burgard, Priti Pharkya, Mark J. Burk
Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
Type:
Grant
Filed:
December 13, 2016
Date of Patent:
September 29, 2020
Assignee:
GENOMATICA, INC.
Inventors:
Andreas Schirmer, Baolong Zhu, Emanuela Popova
Abstract: Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
Type:
Grant
Filed:
December 22, 2015
Date of Patent:
July 14, 2020
Assignee:
GENOMATICA, INC.
Inventors:
Lauri H. Suominen, Connor J. Galleher, Michael Japs, Mark J. Burk, Cara Tracewell
Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.
Type:
Grant
Filed:
June 16, 2014
Date of Patent:
July 14, 2020
Assignee:
GENOMATICA, INC.
Inventors:
Andreas W. Schirmer, Haibo Wang, Stephen B. Del Cardayre, Zhihao Hu, Louis G. Hom, Baolong Zhu, Cindy Chang, Emanuela E. Popova
Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.
Type:
Grant
Filed:
July 17, 2015
Date of Patent:
June 9, 2020
Assignee:
GENOMATICA, INC.
Inventors:
Andreas W. Schirmer, Noah Helman, Haibo Wang, Zhihao Hu, Vikranth Arlagadda, Alma Itzel Ramos-Solis, Elizabeth Clarke
Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.
Type:
Grant
Filed:
July 2, 2015
Date of Patent:
June 2, 2020
Assignee:
GENOMATICA, INC.
Inventors:
Stephanie J. Culler, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.
Type:
Grant
Filed:
May 4, 2018
Date of Patent:
May 26, 2020
Assignee:
Genomatica, Inc.
Inventors:
Jun Sun, Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Wei Niu, John D. Trawick, Robert Haselbeck
Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.
Type:
Grant
Filed:
May 9, 2018
Date of Patent:
May 26, 2020
Assignee:
Genomatica, Inc.
Inventors:
Warren Clark, Michael Japs, Mark J. Burk
Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.
Type:
Grant
Filed:
February 23, 2018
Date of Patent:
May 5, 2020
Assignee:
Genomatica, Inc.
Inventors:
Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.
Type:
Grant
Filed:
August 27, 2013
Date of Patent:
April 21, 2020
Assignee:
Genomatica, Inc.
Inventors:
Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.
Abstract: The disclosure relates to acetyl-CoA carboxylase (ACC) variants and host cells expressing them for the production of malonyl-CoA derived compounds including fatty acid derivatives. Further contemplated are methods of producing increased amounts of malonyl-CoA derived compounds and related cell cultures.
Type:
Grant
Filed:
September 12, 2014
Date of Patent:
March 31, 2020
Assignee:
GENOMATICA, INC.
Inventors:
Derek L. Greenfield, Donald E. Trimbur, Andreas W. Schirmer, Cindy Chang, Behnaz Behrouzian, Jessica Winger