Abstract: The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.

Abstract: Aspects of the invention are directed to systems and methods for generating spectral computed tomography data for spectral X-ray image reconstruction using of pixelated k-edge apertures. A method is provided for generating a spectral computed tomography. The method includes the steps of generating a plurality of X-ray beams; encoding the plurality of X-ray beams by transmitting the plurality of beams through a pixelated K-edge coded aperture structure; detecting the encoded plurality of X-ray beams; and reconstructing a spectral CT image from the encoded plurality of X-ray beams.

Abstract: Medical treatment simulation systems and devices are disclosed. One device includes an overlay, a simulated treatment structure, at least one feedback device, and at least one processor. The overlay is configured to be secured to the live subject and to cover at least a portion of a body of the live subject. The simulated treatment structure is configured to simulate a structure associated with the medical procedure. The at least one feedback device is configured to provide a feedback signal to the live subject. The at least one processor is connected to the simulated treatment structure and the at least one feedback device. The processor is programmed to to operate the feedback device to provide the feedback signal based upon input generated from interaction between a treatment provider and the simulated treatment structure. The disclosed devices may be used to simulate intravenous, catheter, defibrillation, and/or thoracic treatments.

Abstract: Methods that include acylating an aromatic containing compound by reacting the aromatic containing compound with an anhydride containing compound to form an acylated aromatic containing compound.

Abstract: Disclosed herein is a device comprising a PHA based copolymer layer comprising at least one of an electrospun ribbon of fibers of a polyhydroxyalkanoate based copolymer or the polarized polymeric composition obtained by the process of claim 1, wherein the layer is configured to exhibit one or more of a piezoelectric effect, a pyroelectric effect and a ferroelectric effect, wherein each of the electrospun ribbon of fibers and the polarized polymeric composition comprises a ?-form of the PHA based copolymer present in an amount of from about 10% to about 99%, as measured by x-ray diffraction. The device can be configured for use as a sensor, a actuator, a nanomotor, or a biobattery.

Abstract: Skeletonized blood vessels for use as vascular grafts are protected from biomechanical injury and/or certain cellular and extracellular changes by application of a biocompatible hydrogel to the vessel exterior. The hydrogel may be applied to the vessel graft before or after harvesting from a donor patient.

Abstract: Light representing a scene is directed through a lens module coupled to an imaging sensor. The lens module includes: first and second cylindrical lenses positioned along an optical axis of the imaging sensor, and first and second slit-shaped apertures disposed on the respective first and second cylindrical lenses. A cylindrical axis of the second cylindrical lens is arranged at an angle away from parallel with respect to a cylindrical axis of the first cylindrical lens. The light directed through the lens module is captured by the imaging sensor to form at least one multi-perspective image. The at least one multi-perspective image is processed to determine a reconstruction characteristic of the scene.

Abstract: Medical treatment simulation systems and devices are disclosed. One device includes an overlay, a simulated treatment structure, at least one feedback device, and at least one processor. The overlay is configured to be secured to the live subject and to cover at least a portion of a body of the live subject. The simulated treatment structure is configured to simulate a structure associated with the medical procedure. The at least one feedback device is configured to provide a feedback signal to the live subject. The at least one processor is connected to the simulated treatment structure and the at least one feedback device. The processor is programmed to operate the feedback device to provide the feedback signal based upon input generated from interaction between a treatment provider and the simulated treatment structure. The disclosed devices may be used to simulate intravenous, catheter, defibrillation, and/or thoracic treatments.

Abstract: The present invention provides a method for increasing production of a metabolite by a non-naturally occurring methylotroph, comprising growing the non-naturally occurring methylotroph in a medium comprising methanol. Expression of one or more native genes in the non-naturally occurring methylotroph is changed. Also provided are the non-naturally occurring methylotroph and preparation thereof.

Abstract: The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.

Abstract: The present invention relates to a calibration system and method including a pixelated display including pixels for projecting an image, an imager positioned to capture the image produced by the pixelated display, and a processor. The processor is configured to determine and measure non-uniformity in the image produced by the pixelated display by repeatedly selecting and illuminating a subset of the pixels in the pixelated display with respective driving currents, controlling the imager to capture the image projected by the subset pixels, determining and storing, for each pixel in the subset, an intensity value produced in response to the respective driving current, and updating, for each pixel in the subset, the respective driving currents based on the determined intensity value and previously stored intensity values corresponding with previously stored driving currents.

Abstract: A Diels-Alder adduct of a trans-cyclooctene with a tetrazine is provided, wherein the adduct bears a substituent labeled with a radionuclide. A method of producing a PET or other image of an organ in an animal or human includes forming the Diels-Alder adduct in the animal or human. Trans-cyclooctenes and tetrazines suitable for preparing the adducts are provided.

Abstract: A method for mitigating an involuntary muscle movement in a patient by applying external vibration in multiple locations distributed about a periphery of an upper arm or forearm of the patient for an applied vibration duration that is less than the efficacy duration. Applied vibration duration as a percentage of efficacy duration is ideally less than 80%. A wearable device for performing the method has a flexible base for removably affixing to a patient's upper arm or forearm, a plurality of vibration generators, a power source, and a connected microcontroller configured to control the vibration generated by the vibration generators in accordance with a pulse frequency. The microcontroller is configured to connect to a remote controller via a communications interface. The vibration generators have a nominal vibration frequency different from the pulse frequency.

Abstract: The present invention provides a recombinant eukaryotic cell expressing one or more heterologous double strand break (DSB) repair proteins in an amount effective for enhancing DSB repair in the cell. The recombinant eukaryotic cell may express a recombinant product of interest. Also provided are methods for enhancing double strand break (DSB) repair in eukaryotic cells, establishing host cells for production of a recombinant product of interest, producing a recombinant product of interest, improving production of a recombinant product of interest by eukaryotic cells, and/or investigating suitability of eukaryotic cells as host cells for producing a recombinant product of interest.

Abstract: Medical treatment simulation devices are disclosed. One device includes an overlay configured to be secured to a subject, a simulated treatment structure, at least one detector, and at least one feedback device. The detector is coupled to the simulated treatment structure and configured to detect an interaction with the simulated treatment structure. The feedback device is coupled to the overlay and configured to provide feedback based on the interaction detected by the at least one detector.

Abstract: Conductive, elastic ionic polymers with high tensile strength (iono-elastomers) resulting from amphiphilic polymers capable of self-assembly into micellar hierarchical gel-like structures in an electrically conductive liquid are provided. The micellar hierarchical gel-like structures engage in micellar corona cross-linking to form the iono-elastomers. The iono-elastomer exhibits high stretchability and displays increased electrical conductivity upon mechanical stretching. A method for producing iono-elastomers includes mixing amphiphilic polymers in a conductive liquid to form micellar hierarchical gel-like structures, and cross-linking the micellar coronas. Devices incorporating iono-elastomers and methods for creating such devices are also provided. The devices incorporating the iono-elastomers may be wearable.

Abstract: The present disclosure is directed to a process for preparing silahydrocarbons of formula (I): the process including the step of reacting a compound of formula (II): R1-MX??(II) with a compound of formula (III): as well as to silahydrocarbons prepared by such a process, and to compositions and articles of manufacture containing such silahydrocarbons.

Abstract: Syntrophic co-cultures containing at least two microorganisms, wherein (a) at least one of the microorganisms is a solventogen able to metabolize biomass to produce metabolic byproduct(s) therefrom, (b) at least one of the microorganisms is a microorganism different from the solventogen, wherein the microorganism different from the primary solventogen depends on the metabolites and/or the metabolism of the primary solventogen for survival and growth and is able to fix or metabolize the metabolic byproducts produced by the solventogen to produce metabolic byproduct(s) therefrom, and (c) the solventogen is able to metabolize the metabolic byproduct(s) produced by the microorganism different from the solventogen to produce further metabolic byproducts, such as liquid fuels and commodity chemicals, as well as methods for using these syntrophic co-cultures to produce such products via fermentation, are disclosed.

Abstract: Dimethoxyphenol-based monomers containing polymerizable functional groups such as [meth]acrylate groups are useful for the preparation of polymers, wherein one or more dimethoxyphenyl moieties are part of side chains pendant to the backbones of the polymers. The polymers thereby obtained may have different, improved properties, such as higher glass transition temperatures, thermal stability and solvent resistance, as compared to polymers based on other types of lignin-derived monomers.

Abstract: The present disclosure is directed to stress-responsive compositions comprising (1) at least one (co)polymer comprising at least one mechanophore comprising at least one thiocarbonylthio functional group or derivative thereof and (2) at least one compound comprising at least one functional group capable of reacting with a free radical, The present disclosure is also directed to articles, coatings, and 3D printing binders comprising such stress-responsive compositions, as well as to processes of imparting the abilities of forming and maintaining protective barriers and mechanical self-healing to such articles, coatings, and 3D printing binders by incorporating such stress-responsive compositions therein.