Abstract: A syringe for dispensing a flowable material. The syringe has a cartridge with a cartridge front end and at least a first dispensing nozzle arranged at the cartridge front end. The syringe further has closure for openably closing the first dispensing nozzle. The closure includes a cap and at least a first seal. The cap is telescopically placeable on the cartridge front end. The closure or the cartridge has a first magnet for establishing a magnetic attraction between the closure and the cartridge. In a situation in which the closure is placed on the cartridge front end, the closure and the cartridge can be positioned in a sealing position in which the first seal spaces the cap and the cartridge front end away from each other against a magnetic attraction that urges the cap and the cartridge toward each other.

Abstract: A removable dental appliance may include an appliance body at least partially surrounding a plurality of teeth and an interproximal reinforcement. The appliance body may include a first shell shaped to engage a first tooth and a second shell shaped to engage a second tooth. The interproximal reinforcement extends from a first gingival edge on a labial side of the appliance body along an interproximal region between the first tooth and the second tooth to a second gingival edge on a lingual side of the appliance body. The interproximal reinforcement is configured to engage at least one of a lingual surface or labial surface of the first tooth below a height of contour to enable the appliance body to cause movement of the first tooth toward a desired position.

Abstract: A packaging and applicator device comprising a container containing a liquid composition for application, an applicator capable of being fastened removably on the container, and a corrector mounted at one end of the applicator. The applicator includes a rod, an applicator endpiece at a distal end of the rod, and a handle member connected to a proximal end of the rod. The applicator endpiece is made of a felt tip that can be impregnated with the composition contained in the container, and wherein the applicator endpiece is used for applying eyeliner. The packaging and applicator device further includes a filter adjacent to the applicator endpiece to supply the liquid composition to the applicator endpiece during application.

Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an ?-olefin, such as 1-hexene or butadiene. The present invention also provides for a host cell comprising the PKS and when cultured produces the ?-olefin.

Abstract: A method for processing a stream of cellulosic fines containing inorganic particles, to increase a hydrolysis yield of polysaccharide degradation enzymes, such fines in a waste stream from a recycled packaging paper mill to produce a stream of fermentable sugars, comprising treating the fines with a surfactant which selectively binds to the inorganic particles and which reduces binding to the inorganic particles by the polysaccharide degradation enzymes, and degrading polysaccharides in the waste stream with the polysaccharide degradation enzymes.

Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing enzymes or enzyme domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing enzymes or domains, are provided.

Abstract: Provided are methods for synthesizing compounds, including chiral kynurenine compounds. The methods are suitable for large-scale manufacture and produce the chiral kynurenines compounds in high chemical purity and high chiral purity.

Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.

Abstract: The present invention describes the novel molecular entities, nanodisc clathrates, the method of preparation, and the use of these molecular entities for solution phase analysis or crystallization.

Abstract: Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like ?-amylase, e.g., a Bacillus amylase. In the hybrid, an N-terminal portion of a Termamyl-like ?-amylase is replaced with sequences from an archae ?-amylase. The sequence similarity between the two amylase sequences may be less than 60%. Conserving the wild-type 3D structure in the hybrid facilitates obtaining enzymatically active amylases. In one embodiment, one or both amylase sequences contribute residues to the B domain, resulting in particularly advantageous properties. For instance, replacement of the Ca2+ binding site in the B domain of the Termamyl-like ?-amylase with a B domain sequence of an archae ?-amylase that does not bind Ca2+ can produce a hybrid that is fully active in the absence of Ca2+.

Abstract: The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.

Abstract: The application describes a method for producing alkenes (for example propylene, ethylene, 1-butylene, isobutylene, isoamylene, butadiene or isoprene) from 3-hydroxy-1-carboxylic acids via 3-sulfonyloxy-1-carboxylic acids.

Abstract: Provided are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of primer extension methods. Also provided are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

Abstract: The present invention provides novel methods for preparing glycosylated molecules such as oligosaccharides, glycolipids, and glycoproteins/peptides. Novel sialyltransferases are also disclosed. The method includes forming a reaction mixture containing an acceptor molecule, a donor substrate having a sugar moiety and a nucleotide, and a sialyltransferase selected from PmST3 (SEQ ID NO:7) and certain variants thereof. The reaction mixture is formed under conditions sufficient to transfer the sugar moiety from the donor substrate to the acceptor molecule, thereby forming the glycosylated molecule. In some embodiments, the acceptor molecule is selected from a natural product, an oligosaccharide, a glycoprotein, and a glycolipid. In some embodiments, the donor substrate is formed via conversion of a suitable hexosamine derivative to a cytidine 5?-monophosphate(CMP)-sialic acid in a one-pot reaction mixture containing asialic acid aldolase and a CMP-sialic acid synthetase.

Abstract: Disclosed are methods for producing optically active amino acids and amines. According to the methods, ?-keto acids are generated as reaction intermediates, and as a result, ?-transaminase-catalyzed kinetic resolution of racemic amino acids or amines as racemic amine compounds enables the production of optically active amine compounds without the need to use expensive ?-keto acids as starting materials. Therefore, the optically active amine compounds are produced at greatly reduced costs. In addition, the optically active amine compounds have high enantiomeric excess.

Abstract: Provided is a method for producing bioproducts, including the steps of: culturing a first microorganism to produce bioalcohol; hydrolyzing the first microorganism; separating the bioalcohol; obtaining wastes from the hydrolyzed bioalcohol fermentation; and inoculating a second microorganism into the wastes from the hydrolyzed bioalcohol fermentation.

Abstract: The present invention is intended to prove a technique useful for controlling the reaction of oxidoreductase, and to provide a reaction system allowing efficient conversion from carbon dioxide to formic acid, and an efficient methanol production system including the reaction system. The reverse redox reaction is selectively promoted by carrying out the reaction catalyzed by an oxidoreductase using an artificial electron carrier. The reaction system is used for the production of methanol.

Abstract: Nucleases and methods of using these nucleases for expressing a transgene from a safe harbor locus in a secretory tissue, and clones and animals derived therefrom.

Abstract: A process for producing alanine, including culturing a cell under aerobic conditions in an aqueous phase in the presence of an inorganic nitrogen source, and then contacting the aqueous phase and the cell cultured in the aqueous phase with a hydrophobic organic phase. The cell is a prokaryotic or a lower eukaryotic cell and expresses a recombinant alanine dehydrogenase.

Abstract: The present invention concerns a method for enhancing the growth rate, the biomass production and/or the fermentative potential of an anaerobic microorganism, comprising the steps consisting in: (a) modifying said anaerobic microorganism by inactivating the ohrR gene, the ohrR gene product or homolog thereof; (b) culturing the modified anaerobic microorganism obtained in step (a) under low-ORP anaerobic conditions.