Abstract: Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a ?-ketoacyl acyl carrier protein synthase.
Type:
Grant
Filed:
July 19, 2013
Date of Patent:
May 16, 2017
Assignee:
Codexis, Inc.
Inventors:
Jonathan Vroom, Svetlana Balatskaya, Yoram Barak, Louis Clark, Trish Choudhary, Kristian Karlshoej, Fernando Valle, Kaman Chan
Abstract: Provided herein are integrated continuous biomanufacturing processes for producing a therapeutic protein drug substance. Also provided are systems that are capable of continuously producing a therapeutic protein drug substance.
Type:
Grant
Filed:
March 3, 2014
Date of Patent:
May 16, 2017
Assignee:
Genzyme Corporation
Inventors:
Konstantin Konstantinov, Rahul Godawat, Veena Warikoo, Sujit Jain
Abstract: Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.
Type:
Grant
Filed:
June 29, 2012
Date of Patent:
May 16, 2017
Assignee:
INVISTA NORTH AMERICA S.A.R.L.
Inventors:
Paul S. Pearlman, Changlin Chen, Adriana L. Botes, Alex Van Eck Conradie, Benjamin D. Herzog
Abstract: Provided herein are integrated continuous biomanufacturing processes for producing a therapeutic protein drug substance. Also provided are systems that are capable of continuously producing a therapeutic protein drug substance.
Type:
Grant
Filed:
February 20, 2015
Date of Patent:
May 16, 2017
Assignee:
Genzyme Corporation
Inventors:
Konstantin Konstantinov, Rahul Godawat, Veena Warikoo, Sujit Jain
Abstract: A fusion protein comprising factor VII (FVII) and transferrin according to the present invention has an improved specific activity of FVII compared to existing FVII fusion proteins comprising other fusion partners than transferrin, and thus can be effectively used in a therapy using FVII.
Type:
Grant
Filed:
June 7, 2011
Date of Patent:
May 9, 2017
Assignee:
SK Chemicals Co., Ltd.
Inventors:
In-Young Song, Hun-Taek Kim, Bong-Yong Lee, Mahn-Hoon Park, Ho-Soon Lee, Yun Jung Lim, Ji-Hye Lee, Seo Yeon Son, Min-Sun Kim
Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.
Type:
Grant
Filed:
October 27, 2010
Date of Patent:
May 9, 2017
Assignee:
SPIBER TECHNOLOGIES AB
Inventors:
Jan Johansson, Anna Rising, My Hedhammar, Kerstin Nordling
Abstract: The present invention relates to methods and compositions for increasing production of 3-methyl-2-butenol in a de novo synthetic pathway in a genetically modified host cell using isopentenyl disphosphate (IPP) as a substrate.
Type:
Grant
Filed:
September 13, 2012
Date of Patent:
April 25, 2017
Assignee:
The Regents of the University of California
Abstract: A method for the purification of immunoglobulins by ion exchange chromatography is described. The chromatographic method uses a weak ion exchange resin and a single step elution process for the purification of an immunoglobulin. Additionally a method for the determination of the salt concentration for the single step elution of an immunoglobulin from an ion exchange resin is described.
Type:
Grant
Filed:
May 6, 2014
Date of Patent:
April 25, 2017
Assignee:
HOFFMANN-LA-ROCHE INC.
Inventors:
Roberto Falkenstein, Burkard Kolb, Maria Sebald
Abstract: A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition.
Abstract: Methods of increasing the amount of polyunsaturated fatty acids (PUFAs) in the total lipid fraction and in the oil fraction of PUFA-producing, oleaginous eukaryotes, accomplished by modifying the activity of peroxisome biogenesis factor (Pex) proteins. Disruptions of a chromosomal Pex3 gene, Pex10p gene or Pex16p gene in a PUFA-producing, oleaginous eukaryotic strain resulted in an increased amount of PUFAs, as a percent of total fatty acids and as a percent of dry cell weight, in the total lipid fraction and in the oil fraction of the strain, as compared to the parental strain whose native Pex protein was not disrupted.
Type:
Grant
Filed:
July 22, 2014
Date of Patent:
April 11, 2017
Assignee:
E I DU PONT DE NEMOURS AND COMPANY
Inventors:
Seung-Pyo Hong, Pamela L Sharpe, Zhixiong Xue, Narendra S Yadav, Quinn Qun Zhu
Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
April 4, 2017
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Dennis Flint, Brian James Paul, Rick W. Ye
Abstract: Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.
Type:
Grant
Filed:
April 25, 2013
Date of Patent:
March 21, 2017
Assignee:
REG LIFE SCIENCES, LLC
Inventors:
Jay D. Keasling, Zhihao Hu, Chris Somerville, George Church, David Berry, Lisa C. Friedman, Andreas Schirmer, Shane Brubaker, Stephen B. Del Cardayre
Abstract: The present invention provides a method for conveniently and inexpensively producing an amino-hydroxybenzoic acid-type compound such as 3-amino-4-hydroxybenzoic acid by utilizing Escherichia coli that is a bacterium commonly used for a production process by biosynthesis. Specifically, the present invention provides an Escherichia coli having an ability to produce 3-amino-4-hydroxybenzoic acid, which is modified to reduce an activity of N-hydroxyarylamine O-acetyltransferase (NhoA); a method for producing a 3-amino-4-hydroxybenzoic acid-type compound using such Escherichia coli; and the like.
Abstract: A method for producing ethanol from lignocellulosic biomass using yeast at low cost is provided. The method of the present invention for producing ethanol from lignocellulosic biomass includes steps of (1) pretreating lignocellulosic biomass, (2) treating a cellulose fraction obtained in Step (1) with a cellulose hydrolase, (3) mixing saccharified biomass obtained in Step (2) with yeasts to perform ethanol fermentation, and (4) subjecting a fermentation product obtained in Step (3) to a solid-liquid separation, wherein a cycle consisting of Steps (1), (2), (3) and (4) is repeated twice or more, and yeasts obtained in Step (4) are used as all or a portion of yeasts in Step (3) of the subsequent cycle.
Type:
Grant
Filed:
March 21, 2013
Date of Patent:
February 28, 2017
Assignees:
KANSAI CHEMICAL ENGINEERING CO., LTD., BIO-ENERGY CORPORATION
Abstract: This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non-ribosomal peptide synthesis. Methods of treating cancer are also disclosed.
Type:
Grant
Filed:
December 17, 2010
Date of Patent:
February 28, 2017
Assignee:
Gene Bridges GmbH
Inventors:
Youming Zhang, Jun Fu, Xiaoying Bian, Adrian Francis Stewart, Rolf Müller
Abstract: The present invention relates to novel bacteria and the uses thereof. The invention particularly relates to bacteria having a metabolic pathway ratio between pentose phosphate and glycolysis greater than 0.5, and their uses in the chemical, pharmaceutical and agro-chemical industries, e.g., for producing compounds of industrial interest.
Type:
Grant
Filed:
December 21, 2012
Date of Patent:
February 21, 2017
Assignee:
DEINOVE
Inventors:
Jean-Paul Leonetti, Juha-Pekka Pitkanen, Paula Jouhten
Abstract: A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition.
Abstract: Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
Type:
Grant
Filed:
February 20, 2012
Date of Patent:
January 10, 2017
Assignee:
Roche Diabetes Care, Inc.
Inventors:
Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
Abstract: The present invention relates compositions and methods for identifying, isolating, and characterizing herbicide tolerant mutations in monocot plastidic acetyl-CoA carboxylases using a model system.
Type:
Grant
Filed:
November 13, 2012
Date of Patent:
January 10, 2017
Assignee:
BASF SE
Inventors:
Leon Neuteboom, Sherry R. Whitt, John A. McElver, Jill M. Stevenson-Paulik, S. Luke Mankin