Abstract: An antibacterial agent characterized by containing a polyelectrolyte complex prepared by reacting a cationic polymer containing N.sup.+ atoms in repeating units thereof and an anionic polymer containing --COO.sup.--, --SO.sub.3.sup.--, or --PO.sub.3.sup.-- groups in repeating units thereof, and an antibacterial material carrying the above polyelectrolyte complex on a carrier.

Abstract: The present invention relates to certain antibiotic compounds, designated N787-182 compounds herein and derivatives thereof, which are antiparasitic agents, active against insect pests, acari, free-living nematodes and endo- and ectoparasites. This invention also relates to pharmaceutical and other compositions containing such compounds, methods of using such compounds, the microorganism Streptomyces hygroscopicus ATCC 53718 and mutants or genetically transformed or recombinant form thereof, and processes for producing such compounds.

Abstract: Echinocandin B deacylase is purified to near homogeneity from Actinoplanes utahensis by a process comprising, in order, extracting the soluble enzyme, heating to 60.degree. C., hydrophobic interaction chromatography, (NH.sub.4).sub.2 SO.sub.4 fractionation, gel filtration, cation exchange chromatography, dye-ligand chromatography, gel filtration, and cation-exchange chromatography.

Abstract: A method for removing dipeptides from the amino terminus of precursor polypeptides to produce a polypeptide product is presented which comprises contacting the precursor polypeptide for sufficient time to remove the dipeptide with an immobilized dipeptidylaminopeptidase (dDAP) from the slime mold, Dictyostelium discoideum, which has a mass of about 225 kilodaltons and a pH optimum of about 3.5. The precursor polypeptides may be made recombinantly and may be analogs of naturally occurring polypeptides.

Abstract: Surgical repair of diseased or damaged endogenous connective tissue can be accomplished using a tissue graft formed from a delaminated segment of intestinal tissue. The tissue graft comprises the intestinal tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa. The graft can be conditioned by stretching and formed as a multilayer composition for high tensile strength and resistance to tearing at its points of attachment to existing physiological structures.

Abstract: An artificial plasma-like substance having at least one water soluble polysaccharide oncotic agent selected from the group consisting of high molecular weight hydroxyethyl starch, low molecular weight hydroxyethyl starch, dextran 40 and dextran 70, and albumin which is buffered by lactate and has a pre-administration pH of between 5 and 6.5 is disclosed. Also disclosed is an artificial plasma-like solution having at least two water soluble polysaccharide oncotic agents one of which is eliminated from the circulation slowly and the other of which is eliminated from the circulation quickly. Supplimentation of the plasma-like solution with certain ions is described. A system for administration of the plasma-like solution to a subject wherein the system comprises a first and second solution each having particular buffers is described. The plasma-like solution including cryoprotective adducts is also disclosed.

Abstract: A novel multistem strain of Lyophyllum ulmarium to form fruiting bodies is disclosed. The cultivation of the novel strain of Lyophyllum ulmarium is achieved by inoculating a medium with a novel strain of Lyophyllum ulmarium and allowing the formation of fruit bodies, the novel strain of Lyophyllum ulmarium being one capable of giving 65 or more, on the average, of fruiting bodies of a stem length of 20 mm or longer when 10 g of a solid inoculum thereof is inoculated into a medium. The medium is prepared by mixing sawdust (an equivolumetric mixture of sawdust from cedar and that from beech) and rice bran at a ratio by volume of 3:1, adjusting the moisture content to 63% and press-bottling into a polypropylene wide-mouthed bottom (volume: 850 ml) in such a manner as to give 535 g of the contents, whereby fruiting bodies are formed in a conventional manner. Three strains of the multistem strain are provided.

Abstract: A method for using thiazine dyes, especially methylene blue, alone or in combination with low levels of light, to selectively inactivate or inhibit intracellular replication of specific viruses, especially human immunodeficiency virus in vitro and in vivo. Examples of useful thiazine dyes are methylene blue, azure A, azure C, toluidine blue O, and thionine. The preferred dye at this time is methylene blue. Methylene blue is FDA approved for topical, i.v., and oral administration, and has minimal side effects. Since methylene blue absorbs in the red wavelengths, i.e., approximately 670 nm, which penetrates tissue much better than other lower wavelengths, light penetrating the skin to the capillaries at the surface can be used to enhance the activity of the dye.

Abstract: Described is a novel screening method for detecting an abnormal platelet condition in blood, and a kit for use in such a method. An initial screen for an abnormal platelet condition in blood applies to a non-interfering test surface a platelet rich plasma specimen from the blood and an aqueous reagent including a hydroxy-substituted aromatic compound (preferrably propyl gallate) and a metal ion, (such as Ni.sup.2+, Co.sup.2+, or Cu.sup.2+) in concentrations sufficient to cause platelet aggregation in a normal platelet rich plasma sample upon agitation, lightly agitating the platelet rich plasma specimen in contact with the reagent, and visually detecting for the presence of platelet aggregates in the specimen.

Abstract: Pharmaceutical compositions for delivering an effective dose to a desired site of a heparinase. These compositions are based on the discovery that heparinase alone can inhibit angiogenesis. The effective dosage is dependent not only on the heparinase, but also on the method and means of delivery, which can be localized or systemic. For example, in some applications, as in the treatment of psoriasis or diabetic retinopathy, the inhibitor is delivered in a topical ophthalmic carrier. In other applications, as in the treatment of solid tumors, the inhibitor is delivered by means of a biodegradable, polymeric implant.

Abstract: Methods and artificial matrices for the growth and implantation of urological structures and surfaces are disclosed in which urothelial cells are grown in culture on biodegradable, biocompatible, fibrous matrices formed of polymers, such as polyglycolic acid, polylactic acid, or other polymers which degrade over time. The cells can be cultured in vitro until an adequate cell volume and density has developed for the cells to survive and proliferate in vivo. Alternatively, when adequate cell numbers for implantation are available, the cells can be attached to the matrix and implanted directly, without proliferation in vitro. The implants approximate the desired urological structure to be replaced or repaired, such as the kidney, urether, bladder, urethra, and the like. Implantation is followed by remodeling through cell growth and proliferation in vivo.

Abstract: Disclosed herein are antifoaming agents for fermentation which include, as active ingredient at least one of (a) a reaction product obtained by adding at least one alkylene oxide to a mixture of a fat and/or oil with a trihydric or still higher polyhydric alcohol, or (b) a compound represented by the following general formula (1): ##STR1## wherein n stands for a number of 2-50, R.sup.1, R.sup.2 and R.sup.3 mean individually a hydrogen atom or an acyl group having 2-31 carbon atoms, x denotes an alkylene group having 2-4 carbon atoms, and m1, m2 and m3 are individually a number of 0-200, an L-amino acid-producing medium containing either one of these components, and a production process for L-amino acids making use of this medium. The use of this antifoaming agent permits the quick, sure and durable suppression of bubbling during incubation, and the overall production of the L-amino acid is significantly improved.

Abstract: Methods and compositions for the prophylaxis and/or treatment of sexually transmitted diseases (STDs) are disclosed, in which amounts of a haloperoxidase, such as myeloperoxidase or eosinophil peroxidase, and a semen substrate-specific oxidase are administered to a human or animal subject in the environment of sexually transmitted fluids. In the presence of semen, the semen substrate-specific oxidase catalyzes the production of hydrogen peroxide, which in turn is utilized by the haloperoxidase to selectively inhibit pathogenic microbes present in the sexually transmitted fluids. At high concentration levels, the compositions additionally exhibit spermicidal properties.

Abstract: The present invention relates to a new strain of Sporothrix flocculosa Arcc 74320 resistant to the fungicide dodemorph-acetate and its use as a biofungicide in integrated pest management with the fungicide.

Abstract: A process for the production of dulcitol by fermentative reaction of lactose by galactokinase-negative mutants of genus Kluyveromyces is described, which is characterized in that5 g to 25 g of glucose is added to the fermentation culture after substantial hydrolysis of the lactose per liter orthe fermentation is performed under the conditions of the resting-cell process.

Abstract: Prophylactic and therapeutic agents for the prevention and treatment of viral-induced tumors and, in particular, compositions derived from an Aspergillus fermentation extract, and the use thereof, either directly or to prepare a medicament, for the prevention and treatment of viral-induced tumors in mammals. Such tumors include papilloma-induced tumors. The composition is topically administered.

Abstract: A method and apparatus for remediating contaminated soil containing organic compounds, wherein, a sectional, portable, and sealable container is assembled at a site for remediation. Contaminated soil for remediation is disposed within the container and covered by a pool of recirculating water carrying selected biological elements and chemicals to effect the remediation process. Hydrocarbon elements released from the contaminated soil rise to the surface of the pool and are collected by skimming for recovery.

Abstract: This invention provides a method of preventing or treating hyperproliferative vascular disease in a mammal by administering an antiproliferative effective amount of rapamycin alone or in combination with mycophenolic acid.

Abstract: A method is provided for increasing the fertilization potential of oocytes comprising culturing oocytes in vitro with an effective amount of inhibin, activin, or a combination of inhibin and activin. Preferably the oocytes being cultured are immature. After the culturing step, the oocytes can be fertilized. The oocytes are suitably cryopreserved and thawed before the culturing step.

Abstract: A new method of inactivating enveloped viruses and preparations useful in accomplishing this inactivation are disclosed. The method is based on the discovery that paucilamellar lipid vesicles, preferably having non-phospholipids as their primary structural material, can fuse with enveloped virus and that the nucleic acid of the virus denatures shortly after the fusion. The method is useful for inactivating viruses such as orthomyxoviruses, paramyxoviruses, coronaviruses, and retroviruses.