Abstract: Genetically engineered bacteria, pharmaceutical compositions thereof, and methods of treating or preventing autoimmune disorders, inhibiting inflammatory mechanisms in the gut, and/or tightening gut mucosal barrier function are disclosed.

Abstract: Computer-implemented methods for providing improvements in genome-scale metabolic models are described. The methods identify and optimize metabolic flux states that minimize the cost of enzyme production while maximizing a desired cellular phenotype. The computer-implemented methods may maximize cellular phenotypes such as growth (biomass) or production of a metabolite, such as a commercially valuable chemical compound, through the selection of metabolic pathways that maximize these phenotypes while minimizing metabolic costs associated with production of the proteomic constituents of individual metabolic pathways. The computer implemented methods may be useful for computationally designing microbial strains for the production of chemicals.

Abstract: Methods including mixing a treatment fluid slurry in a storage tank, the treatment fluid slurry comprising an insulative base fluid, an ambient-reactive subterranean formation additive (ARA), wherein the ARA reacts at an ambient temperature, the reaction resulting in surface modification of the ARA, degradation of the ARA, or a combination of surface modification and degradation of the ARA, and wherein the insulative base fluid maintains the treatment fluid slurry at a temperature different from the ambient temperature to prevent the ARA from reacting; and introducing the treatment fluid slurry into a subterranean formation.

Abstract: Methods including placing particles that are substantially dry in a hopper; conveying the particles from the hopper via a conveyance to an open-air gap; adding a fluid to the particles in the hopper, the particles in the conveyance, or both, thereby reducing an amount of a dust produced at the open-air gap. The particles may be sand, silica, gravel, bauxite, ceramic materials, glass materials, polymer materials, polytetrafluoroethylene materials, nut shell pieces, cured resinous particulates comprising nut shell pieces, seed shell pieces, cured resinous particulates comprising seed shell pieces, fruit pit pieces, cured resinous particulates comprising fruit pit pieces, wood, composite particulates, and combinations thereof.

Abstract: A ditch digging and cleaning apparatus comprising a housing adapted for being moved along a longitudinal axis of the ditch. The housing has an inlet opening at a bottom front portion thereof for transmitting therethrough debris disposed in the ditch and an outlet opening at an upper portion thereof. An impeller is disposed in the housing and rotatably movable mounted thereto. The impeller receives the debris from the inlet opening and propels the same through the outlet opening. A drive is connected to the impeller for driving the same, the drive has a power of at least 30 hp.

Abstract: A reclinable chair is provided which has a seat rest, a backrest; a footrest; two side arm portions; and a reclining mechanism arranged to move at least one of the backrest and the footrest from a substantially upright position into a reclined position. The reclining mechanism is mounted to the side arm portions. The reclining mechanism includes an extendable member which acts on a footrest support to move the footrest from a substantially upright position to a reclined position. A linkage bar may be provided to connect the footrest support to a backrest support so that a single driving mechanism causes movement of the footrest into the reclined position and simultaneously causes movement of the backrest into a reclined position. Alternatively, the linkage bar may be omitted so that only the footrest is reclinable.

Abstract: In accordance with embodiments of the present disclosure, systems and methods for passively reducing or preventing dust formation in a particulate handling system are provided. In some embodiments, the handling system includes a silo for holding bulk material used to form a well fracturing treatment fluid. The silo may include a chute to deposit a portion of the bulk material from the silo into a blender and a cyclone mounted to the silo to separate dust from a pneumatic air flow and to release a substantially clean air into the atmosphere. In other embodiments, the handling system may include a horizontally oriented cyclone assembly mounted to a blender storage tank, cyclone assembly including a horizontally oriented cyclone separator to separate dust from a pneumatic airflow and a dust collection container to receive the dust and output the dust into the storage tank.

Abstract: A molding apparatus for manufacturing a wind turbine blade component includes a main mold body (30) and a flexible bladder (38). The main mold body includes a shape defining surface (32) for receiving composite material forming the blade component and a heat reservoir (40) for heating the blade component during curing. The flexible bladder overlays and conforms to the shape of the blade component and is configured to receive heated liquid for heating the blade component during curing. One or both of the main mold body and the flexible bladder is divided into a plurality of zones (58, 66) that are independently controlled by a controller (70) to maintain a generally uniform temperature of the blade component at each zone.

Abstract: Genetically engineered bacteria, pharmaceutical compositions thereof, and methods of treating or preventing autoimmune disorders, inhibiting inflammatory mechanisms in the gut, and/or tightening gut mucosal barrier function are disclosed.

Abstract: A method and system for chemical transportation, storage, and dispensation, which provides a base with ISO mounts for enabling a ISO tank, van container, screw conveyor, or the like to be carried thereon. The base accommodates either or both of “roll off” or “hooklift hoist” technologies to allow easy loading and unloading. A first end of the base is carried by one or more rollers, and the underside is structured to allow rolling of the base on the bed or rack of the transport vehicle. After unloading the base with its ISO cargo, the transport vehicle leaves the well site and is free to retrieve another ISO tank or container as appropriate, thereby lowering the tractor-trailer capital requirements to support fracturing operations. Weigh scales allow the weight of the ISO container to be determined to allow the operator to monitor the inventory of chemicals as they are used.

Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.

Abstract: Methods including placing particles that are substantially dry in a hopper; conveying the particles from the hopper via a conveyance to an open-air gap; adding a fluid to the particles in the hopper, the particles in the conveyance, or both, thereby reducing an amount of a dust produced at the open-air gap. The particles may be sand, silica, gravel, bauxite, ceramic materials, glass materials, polymer materials, polytetrafluoroethylene materials, nut shell pieces, cured resinous particulates comprising nut shell pieces, seed shell pieces, cured resinous particulates comprising seed shell pieces, fruit pit pieces, cured resinous particulates comprising fruit pit pieces, wood, composite particulates, and combinations thereof.

Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.

Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.

Abstract: A molding apparatus for manufacturing a wind turbine blade component includes a main mold body (30) and a flexible bladder (38). The main mold body includes a shape defining surface (32) for receiving composite material forming the blade component and a heat reservoir (40) for heating the blade component during curing. The flexible bladder overlays and conforms to the shape of the blade component and is configured to receive heated liquid for heating the blade component during curing. One or both of the main mold body and the flexible bladder is divided into a plurality of zones (58, 66) that are independently controlled by a controller (70) to maintain a generally uniform temperature of the blade component at each zone.

Abstract: Computer-implemented methods for providing improvements in genome-scale metabolic models are described. The methods identify and optimize metabolic flux states that minimize the cost of enzyme production while maximizing a desired cellular phenotype. The computer-implemented methods may maximize cellular phenotypes such as growth (biomass) or production of a metabolite, such as a commercially valuable chemical compound, through the selection of metabolic pathways that maximize these phenotypes while minimizing metabolic costs associated with production of the proteomic constituents of individual metabolic pathways. The computer implemented methods may be useful for computationally designing microbial strains for the production of chemicals.

Abstract: An authenticated secure network communication link is established between an alert message generating computer 2 and a destination data processing system 6. The alert message sent specifies a risk threat level and a suggested countermeasure amongst other data. The destination computer 6 automatically responds to the alert message as controlled by its local response configuration parameters to trigger security actions of one or more security mechanisms, such as malware scanners, firewall scanners, security policy managers and the like.

Abstract: Pneumatic particulate material fill systems and methods are disclosed. A particulate material fill system includes a cyclone separator having an inlet adapted to receive pneumatically conveyed particulate material. The cyclone separator has a first outlet adapted to vent air and a second outlet adapted to discharge solids. The particulate material fill system includes a tank adapted to receive solids discharged from the cyclone separator via the second outlet. The tank is adapted for use at atmospheric pressure.

Abstract: An authenticated secure network communication link is established between an alert message generating computer 2 and a destination data processing system 6. The alert message sent specifies a risk threat level and a suggested countermeasure amongst other data. The destination computer 6 automatically responds to the alert message as controlled by its local response configuration parameters to trigger security actions of one or more security mechanisms, such as malware scanners, firewall scanners, security policy managers and the like.