Abstract: A gas extraction method in which high energy gas fracturing technology is used to form a fracture network in a thermal injection borehole. Then high-pressure, cyclically temperature-changing steam is injected into the borehole using a spinning oscillating-pulse jet nozzle to form oscillating superheated steam, alternatingly impacting and heating the coal body. The high energy gas forms a fracture network that provides channels for passage of the superheated steam, while oscillating changes in steam temperature and pressure also promote crack propagation and perforation of the coal body; the combined effect of alternation of the two enhances gas desorption and extraction efficiency.

Abstract: A method for combining integrated drilling and slotting with oscillating thermal injection to enhance coalbed gas extraction, applicable to managing gas extraction from microporous, low-permeability, high-adsorption coal seam areas. A number of slots are formed within a thermal injection/extraction borehole by means of integrated drilling and slotting technology; a steam generator, is then used to three high-pressure, cyclically temperature-changing steam into said borehole; the steam passing through a spinning, oscillating-pulse jet nozzle forms an oscillating superheated steam, heating the coal body. The present method overcomes the limitations of simple permeability-increasing techniques, the slotting by means of hydraulic.

Abstract: A method for permeability improvement for a downhole coal seam by directional fracturing with pulsed detonation waves, which is applicable to gas control in coal seam areas with high gas concentration and low air permeability. The permeability improvement method is as follows: first, drilling a pulsed detonation borehole and pulsed detonation guide boreholes from a coal roadway to a coal seam respectively; then, pushing a positive electrode connected to a positive output side of an explosion-proof high-voltage electrical pulse generator to the bottom of the pulsed detonation borehole and pushing a negative electrode connected to a negative output side of the explosion-proof high-voltage electrical pulse generator to the bottom of the pulsed detonation guide borehole; connecting the pulsed detonation borehole and the pulsed detonation guide boreholes to an extraction pipeline for gas extraction, after electrical pulsed detonation fracturing for the coal seam is carried out.

Abstract: A method for permeability improvement for a downhole coal seam by directional fracturing with pulsed detonation waves, which is applicable to gas control in coal seam areas with high gas concentration and low air permeability. The permeability improvement method is as follows: first, drilling a pulsed detonation borehole and pulsed detonation guide boreholes from a coal roadway to a coal seam respectively; then, pushing a positive electrode connected to a positive output side of an explosion-proof high-voltage electrical pulse generator to the bottom of the pulsed detonation borehole and pushing a negative electrode connected to a negative output side of the explosion-proof high-voltage electrical pulse generator to the bottom of the pulsed detonation guide borehole; connecting the pulsed detonation borehole and the pulsed detonation guide boreholes to an extraction pipeline for gas extraction, after electrical pulsed detonation fracturing for the coal seam is carried out.

Abstract: A motor cooling system for cooling a motor unit including a stator assembly and a rotor assembly configured to rotate a rotor shaft, which includes a stator casing and an arm casing. The stator casing includes a stator casing body having a stator casing wall with an internal cavity to receive the motor unit and a rib with a groove defined between adjacent portions of the rib. The arm casing includes an arm casing body having an internal cavity to receive the stator casing and a port in fluid communication with the groove.

Abstract: The Bacterial cocaine esterase (CocE) mutants disclosed herein each have enhanced catalytic efficiency for (?)-cocaine, as compared to CocE mutants in the prior art, including CocE mutant E172-173. The presently-disclosed subject matter further includes a pharmaceutical composition including a mutant of bacterial cocaine hydrolase, as described herein, and a suitable pharmaceutical carrier. The presently-disclosed subject matter further includes a method of treating a cocaine-induced condition comprising administering to an individual an effective amount of a mutant of bacterial cocaine hydrolase variant, as disclosed herein, to accelerate cocaine metabolism and produce biologically inactive metabolites.

Abstract: A method for combining integrated drilling, flushing and slotting with thermal injection to enhance coalbed gas extraction, applicable to managing gas extraction from microporous, low-permeability, high-adsorption coalbed areas. A gas extraction borehole is drilled within a certain distance of a predetermined drilling, flushing and slotting borehole, and, once sealed, is used for gas extraction. An integrated drilling, flushing and slotting drill bit is used to sink the borehole, which is then sealed. Concentration variation in the gas extraction borehole is monitored in real time, and when concentration is below 30%, borehole is opened and high-temperature steam is injected by means of a steam generator, after which the borehole is again closed.

Abstract: Compounds useful for inhibiting the immunoproteasome have the formula of Methods and compounds for inhibiting the immunoproteasome, particularly, immunoproteasome inhibitors with non-peptide scaffolds, are described.

Abstract: Compounds useful for inhibiting the immunoproteasome have the formula of Methods and compounds for inhibiting the immunoproteasome, particularly, immunoproteasome inhibitors with non-peptide scaffolds, are described.

Abstract: Compounds of formula (I) are useful for inhibiting a proteasome in a cell. Compounds, pharmaceutical compositions and methods of use are provided herein.

Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.

Abstract: Provided are mutant cocaine esterase polypeptides and PEGylated formulations thereof.

Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.

Abstract: Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides.

Abstract: The Bacterial cocaine esterase (CocE) mutants disclosed herein each have enhanced catalytic efficiency for (?)-cocaine, as compared to CocE mutants in the prior art, including CocE mutant E172-173. The presently-disclosed subject matter further includes a pharmaceutical composition including a mutant of bacterial cocaine hydrolase, as described herein, and a suitable pharmaceutical carrier. The presently-disclosed subject matter further includes a method of treating a cocaine-induced condition comprising administering to an individual an effective amount of a mutant of bacterial cocaine hydrolase variant, as disclosed herein, to accelerate cocaine metabolism and produce biologically inactive metabolites.

Abstract: A motor cooling system for cooling a motor unit including a stator assembly and a rotor assembly configured to rotate a rotor shaft, which includes a stator casing and an arm casing. The stator casing includes a stator casing body having a stator casing wall with an internal cavity to receive the motor unit and a rib with a groove defined between adjacent portions of the rib. The arm casing includes an arm casing body having an internal cavity to receive the stator casing and a port in fluid communication with the groove.

Abstract: Glycine, threonine, and serine can enhance the sensitivity of bacteria to serum, plasma, or whole blood, and therefore can be used as molecules to improve the bactericidal effect of the serum, the plasma, or the whole blood.

Abstract: A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, A199S/F227A/S287G/A328W/Y332G, and A199S/F227A/S287G/A328W/E441D all have enhanced catalytic efficiency for (?)-cocaine compared with wild-type BChE.

Abstract: Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides.

Abstract: A respiratory therapy device has both a standard positive expiratory pressure (PEP) device and an oscillatory PEP device packaged together. A manually operable member is movable to select which of the standard and oscillatory PEP device is placed in communication with a patient's airway. A nebulizer connector may also be provided for connection of a nebulizer.