Patents by Inventor Handong Li
Handong Li has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 7176297Abstract: In one aspect of the invention, photoactivatable silane compounds and methods for their synthesis and use are provided. In one embodiment, the photoactivatable silane compounds synthesized are represented by a formula: PG-LS-SN, wherein PG is a photoactivatable group, LS is a linkage and spacer group, and SN is a silane group. The silanes allow the photoactivatable silane compounds to be covalently bound to the surface of a substrate such as silica. The photoactivatable group forms a hydrophobic layer that can be photochemically cross-linked with a layer of hydrophilic functional polymers. In another embodiment, a method is disclosed to synthesize a substrate of hydrophobic layers and hydrophilic functional polymer layers thereafter onto glass surfaces. An array of biopolymers such as nucleic acids and peptides may then be covalently attached to the substrate using photolithography and biopolymer synthesis.Type: GrantFiled: June 28, 2004Date of Patent: February 13, 2007Assignee: Affymetrix, Inc.Inventors: Handong Li, Glenn H. McGall
-
Publication number: 20060257560Abstract: In one aspect of the present invention polymers are used to create films providing three-dimensional array substrates. The films were stable and presented good hydroxyl group numbers as compared with arrays without polymer films. It is an object of the present invention that three dimensional arrays substrates provide a means to obtain higher density polymer arrays.Type: ApplicationFiled: July 13, 2006Publication date: November 16, 2006Applicant: Affymetrix, INC.Inventors: Anthony Barone, Handong Li, Glenn McGall
-
Publication number: 20060148090Abstract: A metal chelating composition having the formula: wherein Q is a carrier; S1 is a spacer; L is -A-T-CH(X)— or —C(?O)—; A is an ether, thioether, selenoether, or amide linkage; T is a bond or substituted or unsubstituted alkyl or alkenyl; X is —(CH2)kCH3, —(CH2)kCOOH, —(CH2)kSO3H, —(CH2)kPO3H2, —(CH2)kN(J)2, or —(CH2)kP(J)2, preferably —(CH2)kCOOH or —(CH2)kSO3H; k is an integer from 0 to 2; J is hydrocarbyl or substituted hydrocarbyl; Y is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; Z is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; and i is an integer from 0 to 4, preferably 1 or 2.Type: ApplicationFiled: March 1, 2006Publication date: July 6, 2006Applicant: Sigma-Aldrich Co.Inventors: William Kappel, Vankatappa Viswanatha, Handong Li, Richard Mehigh, John Dapron
-
Publication number: 20060147965Abstract: A method is provided for label free analysis of nucleic acid materials. In accordance with the present invention, a fluorescent material is provided having a certain fluorescence emission without nucleic acids attached thereto. The fluorescent material of the present invention, has a greater emission upon the binding of a single stranded nucleic acid and an even greater emission upon the binding of a double stranded nucleic acid allowing detection of double stranded binding without using a label attached to the DNA.Type: ApplicationFiled: December 20, 2005Publication date: July 6, 2006Applicant: Affymetrix, INC.Inventors: Andrea Cuppoletti, Handong Li
-
Publication number: 20060147963Abstract: Methods are provided for detecting hybridization of a polynucleotide to a nucleic acid array by chemically modifying the polynucleotide to contain a detectable label. According to one aspect of the present invention, a method is provided for detecting the presence of a mRNA in a nucleic acid sample, the method having the steps of providing a mRNA sample and azido modified nucleotides, hybridizing a primer to the mRNA, reversed transcribing the mRNA to provide azido modified DNA, followed by reacting the azido groups with a detectable label, hybridizing the labeled RNA to a nucleic acid array and detecting the presence of the mRNA.Type: ApplicationFiled: December 20, 2005Publication date: July 6, 2006Applicant: Affymetrix, INC.Inventors: Anthony Barone, Handong Li, Glenn McGall
-
Patent number: 7033520Abstract: A metal chelating composition having the formula: wherein Q is a carrier; S1 is a spacer; L is -A-T-CH(X)— or —C(?O)—; A is an ether, thioether, selenoether, or amide linkage; T is a bond or substituted or unsubstituted alkyl or alkenyl; X is —(CH2)kCH3, —(CH2)kCOOH, —(CH2)kSO3H, —(CH2)kPO3H2, —(CH2)kN(J)2, or —(CH2)kP(J)2, preferably —(CH2)kCOOH or —(CH2)kSO3H; k is an integer from 0 to 2; J is hydrocarbyl or substituted hydrocarbyl; Y is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; Z is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; and i is an integer from 0 to 4, preferably 1 or 2.Type: GrantFiled: September 22, 2003Date of Patent: April 25, 2006Assignee: Sigma-Aldrich Co.Inventors: William K. Kappel, Venkatappa Viswanatha, Handong Li, Richard J. Mehigh, John G. Dapron
-
Publication number: 20050123968Abstract: A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 ?g/cm2.Type: ApplicationFiled: November 5, 2004Publication date: June 9, 2005Applicant: Sigma-Aldrich Co.,Inventors: John Dapron, William Kappel, Handong Li
-
Publication number: 20050119415Abstract: A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 ?g/cm2.Type: ApplicationFiled: November 5, 2004Publication date: June 2, 2005Applicant: Sigma-Aldrich Co.Inventors: John Dapron, William Kappel, Handong Li
-
Publication number: 20050118709Abstract: A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 ?g/cm2.Type: ApplicationFiled: November 5, 2004Publication date: June 2, 2005Applicant: Sigma-Aldrich Co.Inventors: John Dapron, William Kappel, Handong Li
-
Publication number: 20050003496Abstract: Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. Methods for making such heterocyclic compounds are also disclosed. The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.Type: ApplicationFiled: December 23, 2003Publication date: January 6, 2005Applicant: Affymetrix, INC.Inventors: Glenn McGall, Anthony Barone, Handong Li
-
Patent number: 6825032Abstract: A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 &mgr;g/cm2.Type: GrantFiled: May 14, 2001Date of Patent: November 30, 2004Assignee: Sigma-Aldrich Co.Inventors: John Dapron, William Karl Kappel, Handong Li
-
Publication number: 20040234788Abstract: In one aspect of the invention, photoactivatable silane compounds and methods for their synthesis and use are provided. In one embodiment, the photoactivatable silane compounds synthesized are represented by a formula: PG-LS-SN, wherein PG is a photoactivatable group, LS is a linkage and spacer group, and SN is a silane group. The silanes allow the photoactivatable silane compounds to be covalently bound to the surface of a substrate such as silica. The photoactivatable group forms a hydrophobic layer that can be photochemically cross-linked with a layer of hydrophilic functional polymers. In another embodiment, a method is disclosed to synthesize a substrate of hydrophobic layers and hydrophilic functional polymer layers thereafter onto glass surfaces. An array of biopolymers such as nucleic acids and peptides may then be covalently attached to the substrate using photolithography and biopolymer synthesis.Type: ApplicationFiled: June 28, 2004Publication date: November 25, 2004Applicant: Affymetrix, INC.Inventors: Handong Li, Glenn McGall
-
Patent number: 6773888Abstract: In one aspect of the invention, photoactivatable silane compounds and methods for their synthesis and use are provided. In one embodiment, the photoactivatable silane compounds synthesized are represented by a formula: PG-LS-SN, wherein PG is a photoactivatable group, LS is a linkage and spacer group, and SN is a silane group. The silanes allow the photoactivatable silane compounds to be covalently bound to the surface of a substrate such as silica. The photoactivatable group forms a hydrophobic layer that can be photochemically cross-linked with a layer of hydrophilic functional polymers. In another embodiment, a method is disclosed to synthesize a substrate of hydrophobic layers and hydrophilic functional polymer layers thereafter onto glass surfaces. An array of biopolymers such as nucleic acids and peptides may then be covalently attached to the substrate using photolithography and biopolymer synthesis.Type: GrantFiled: July 9, 2002Date of Patent: August 10, 2004Assignee: Affymetrix, Inc.Inventors: Handong Li, Glenn McGall
-
Publication number: 20040092031Abstract: A metal chelating composition having the formula: 1Type: ApplicationFiled: September 22, 2003Publication date: May 13, 2004Applicant: Sigma - Aldrich Co.Inventors: William K. Kappel, Venkatappa Viswanatha, Handong Li, Richard J. Mehigh, John G. Dapron
-
Publication number: 20030194715Abstract: In one aspect of the invention, photoactivatable silane compounds and methods for their synthesis and use are provided. In one embodiment, the photoactivatable silane compounds synthesized are represented by a formula: PG-LS—SN, wherein PG is a photoactivatable group, LS is a linkage and spacer group, and SN is a silane group. The silanes allow the photoactivatable silane compounds to be covalently bound to the surface of a substrate such as silica. The photoactivatable group forms a hydrophobic layer that can be photochemically cross-linked with a layer of hydrophilic functional polymers. In another embodiment, a method is disclosed to synthesize a substrate of hydrophobic layers and hydrophilic functional polymer layers thereafter onto glass surfaces. An array of biopolymers such as nucleic acids and peptides may then be covalently attached to the substrate using photolithography and biopolymer synthesis.Type: ApplicationFiled: July 9, 2002Publication date: October 16, 2003Applicant: Affymetrix, INC.Inventors: Handong Li, Glenn H. McGall
-
Patent number: 6623655Abstract: A metal chelating composition having the formula: wherein Q is a carrier; S1 is a spacer; L is —A—T—CH(X)— or —C(═O)—; A is an ether, thioether, selenoether, or amide linkage; T is a bond or substituted or unsubstituted alkyl or alkenyl; X is —(CH2)kCH3, —(CH2)kCOOH, —(CH2)kSO3H, —(CH2)kPO3H2, —(CH2)kN(J)2, or —(CH2)kP (J)2, preferably —(CH2)kCOOH or —(CH2)kSO3H; k is an integer from 0 to 2; J is hydrocarbyl or substituted hydrocarbyl; Y is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; Z is —COOH, —H, —SO3H, —PO3H2, —N(J)2, or —P(J)2, preferably, —COOH; and i is an integer from 0 to 4, preferably 1 or 2.Type: GrantFiled: April 24, 2000Date of Patent: September 23, 2003Assignee: Sigma-Aldrich Co.Inventors: William K. Kappel, Venkatappa Viswanatha, Handong Li, Richard J. Mehigh, John G. Dapron
-
Publication number: 20030157783Abstract: The invention relates to methods for preparing a removable system on a mother substrate. The method deposits a high surface to volume sacrificial layer on a mother substrate and stabilizes the sacrificial layer by a) removing volatile chemical species in and on the sacrificial layer and/or b) modifying the surface of the layer. The method coats over the sacrificial layer with a capping medium. A system is the fabricated on the capping medium. The method provides through holes to access the sacrificial layer. The method may also apply a top layer onto the system to form a covered system. The invention also includes the step of removing the sacrificial layer to release the system from the mother substrate. Methods of the invention also include selectively removing a portion of the system and capping layers to form void regions defining an array of islands composed of device, structure, or system and capping layer regions, and optionally filling the island-defining void region with a sacrificial material.Type: ApplicationFiled: January 13, 2003Publication date: August 21, 2003Applicant: THE PENN STATE RESEARCH FOUNDATIONInventors: Stephen J. Fonash, Handong Li, Youngchul Lee, Joseph D. Cuiffi, Daniel J. Hayes
-
Publication number: 20030032013Abstract: A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 &mgr;g/cm2.Type: ApplicationFiled: May 14, 2001Publication date: February 13, 2003Inventors: John Dapron, William Karl Kappel, Handong Li