Abstract: The present invention relates to methods of imaging template hybridisation for estimating cluster numbers prior to solid phase amplification and sequencing. More particularly, an initial round of imaging is carried out at the single molecule template hybridisation stage which allows a general estimation of cluster numbers prior to clusters being formed. Amplification of the signal allows single molecule imaging to be carried out using standard sequencing imaging apparatus.

Abstract: An audio filtering and power extraction apparatus for filtering out audio signal and extracting power from a combined signal and direct-current power line, with an input configured to couple to a combined signal and direct-current power line, a filtering extractor system functionally coupled to the input, a DC sensing switch circuit, and a second order low-pass filter functionally coupled to the DC sensing switch circuit, the second order low-pass filter including, an inductor, a capacitor and resistor in series with each other, and an output functionally coupled to an output of the filtering extractor system and through which output extracted power is provided.

Abstract: In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.

Abstract: A voltage converter for an aircraft electrical system includes an input power line configured to receive input electrical power, an output power line configured to supply output electrical power to aircraft loads, conversion circuitry, and a controller. The conversion circuitry is configured to convert the input electrical power to supply the output electrical power based on one or more control parameters. The controller is configured to receive data relating to the aircraft loads and modify at least one control parameter based on the data.

Abstract: A patient interface can have a frame supporting a sealing member. Various features of the sealing member can improve comfort and sealing performance in the context of forming seals with the nares of a user, as well as contact with other facial surfaces. The sealing member can include convex portions, concave portions and thickness variations for providing various sealing, comfort, and deformability effects.

Abstract: Dynamically generating data tests includes: receiving test templates and metadata for a plurality of data objects and, on a trigger condition: based on at least the metadata and the test templates, determining a current set of templated data tests, wherein determining the current set of templated data tests comprises: determining at least one templated data test, from a prior set of templated data tests, to cease using; determining at least one templated data test to add to the current set of templated data tests; and determining, within the current set of templated data tests, at least one templated data tests to regenerate; generating the at least one templated data test to add to the current set of templated data tests; regenerating the least one templated data test to regenerate; and executing templated data tests, within the set of current templated data tests, on the plurality of data objects.

Abstract: Herein provided is an interface for use in transmitting data from an aircraft including a first connector configured for physically and removably coupling a communication module to a communication system which obtains the data on-board the aircraft from at least one data source within the aircraft, and a second connector for digitally coupling the communication module to a processing unit of the communication module producing at least one message for transmission based on the data, the at least one message comprising information of interest identified based on the data. The interface is configured for conducting the at least one message from the processing unit to at least one radio via the second connector.

Abstract: Generally, this disclosure enables various drivers, reversible sockets, and methods of use and manufacture thereof. For example, some of these drivers can avoid laterally rolling off an inclined surface (e.g., between about 15 degrees and about 25 degrees). For example, some of these drivers can avoid using excessive material. For example, some of these drivers can allow for stacking of drivers and thereby be efficient to package for shipping. For example, some of these sockets can provide a tactile feedback for a user to know which ends of those sockets are currently being handled.

Abstract: Systems and methods are disclosed to provide a modulated stimulation signal to a patient, the modulated stimulation signal including a carrier wave modulated with a biological or natural frequency stimulation signal, to provide the benefit of the biological or natural frequency stimulation signal to the patient without the patient perceiving the biological or natural frequency stimulation signal.

Abstract: Surgical systems and methods involve a tracker having at least one trackable component and a localizer configured to track states of the at least one trackable component. An illumination assembly is located remote from the tracker and is operable to direct a visible light. A controller is coupled to the localizer and to the illumination assembly. The controller is configured to detect a condition and control the illumination assembly to remotely direct the visible light at the tracker such that the visible light is reflected by the tracker to communicate the detected condition.

Abstract: The present invention generally relates to golf bags having a lightweight and durable top frame can be made of a lightweight metal and can have different structures. The lightweight top frame assembly can have handle and leg sub-assemblies. Three-dimensional printing methods can be used to form structures in the top frame having various shapes and geometric patterns, for example, a lattice having a top frame (cuff) with club dividers. The walls of the top cuff can be made of a lightweight metal and can have a sidewall, open loop, closed loop, or other structure. The top cuff includes club dividers for storing and organizing clubs in different compartments. The club dividers can be made of a cord material.

Abstract: A subsea tree assembly with a flow monitoring and measuring apparatus includes a production wing valve block coupled to a production wing branch, the production wing valve block including a wing block connector, and a fluid processing module including a frame, a module connector including an inlet and an outlet, and a fluid flow loop coupled between the inlet and the outlet, wherein the module connector is fluidicly coupled to the wing block connector. A production fluid flow goes from the production wing valve block and returns to the same block via the wing block connector, the module connector, and the fluid flow loop. A flow monitoring and measuring apparatus for a subsea tree assembly includes a module frame, a module connector connectable to a production wing valve block, the module connector including an inlet and an outlet, and a fluid flow conduit forming a loop from the outlet of the module connector back to the inlet of the module connector.

Abstract: The present invention relates to the field of molecular biology, and more specifically to methods for reducing the complexity of a nucleic acid sample.

Abstract: An electric motor system that comprises a motor that comprises a rotor having a magnet mounted thereto and a stator that comprises one or more motor phase windings for driving rotation of the rotor when the motor phase windings receive an input voltage from an electrical power supply. The system has a controller that executes a back “EMF” observer that is operable to estimate a rotor angle by observing a back electromotive force induced in the stator by the rotor. Filtering is applied to the back EMF signal to reduce harmonics prior to determining the rotor angle.

Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.

Abstract: Described herein is a polynucleotide for use as a sequencing template comprising multiple inserts. Also described herein are method of generating and using these polynucleotides and methods of use of such templates, including analysis of contiguity information. Further, sequencing templates comprising an insert sequence and a copy of the insert sequence can be used to correct for random errors generated during sequencing or amplification or to identify nucleobase damage or other mutation that leads to non-canonical base pairing in a double-stranded nucleic acid. Methods of performing methylation analysis are also described herein.

Abstract: The present invention provides antibodies or antigen-binding fragment thereof that bind and neutralize TREM1 for use in the treatment of a motor neuron degenerative disorders, in particular amyotrophic lateral sclerosis.

Abstract: A navigation system is disclosed which comprises a tracker having a trackable feature, a localizer configured to track states of the trackable feature within a field of view, an illumination assembly operable to direct light within the field of view, and a controller coupled to the localizer and to the illumination assembly. The controller is configured to receive the tracked states from the localizer, to determine a status condition of the tracker based on the tracked states received from the localizer, and to control the illumination assembly to direct light at the tracker such that light reflected by the tracker from the illumination assembly is visible to a user of the navigation system to communicate the status condition of the tracker.