Patents by Inventor Juhani Saarinen
Juhani Saarinen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20170189549Abstract: The present invention relates to a conjugate represented by the formula [D-L-Y—(CH2)n-O]m—P-T wherein T is a protein; P is a polymer selected from the group consisting of dextran, mannan, pullulan, hyaluronic acid, hydroxyethyl starch, chondroitin sulphate, heparin, heparin sulphate, polyalkylene glycol, Ficoll, polyvinyl alcohol, amylose, amylopectin, chitosan, cyclodextrin, pectin and carrageenan, or a derivative thereof; m is at least 1; n is in the range of 1 to 10; each Y is independently selected from the group consisting of S, NH and 1,2,3-triazolyl, wherein 1,2,3-triazolyl is optionally substituted; each L is independently absent or comprises a linker group covalently joining D and Y; and each D is a payload molecule.Type: ApplicationFiled: June 12, 2015Publication date: July 6, 2017Applicant: Glykos Finland OyInventors: Jari Helin, Juhani Saarinen, Filip S. Ekholm, Anne Leppanen
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Patent number: 9695454Abstract: Described herein are compositions including filamentous fungal cells, such as Trichoderma fungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example, Trichoderma fungal cells, as the expression system.Type: GrantFiled: May 23, 2013Date of Patent: July 4, 2017Assignee: GLYKOS FINLAND OYInventors: Jari Natunen, Ann Westerholm-Parvinen, Hanna Salo, Jussi Joensuu, Heli Viskari, Christopher Landowski, Anne Huuskonen, Anne Kanerva, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Juhani Saarinen, Markku Saloheimo
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Publication number: 20170159094Abstract: The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii.a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing ?1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.Type: ApplicationFiled: July 21, 2015Publication date: June 8, 2017Applicant: GLYKOS FINLAND OYInventors: Jari Natunen, Anne Leppänen, Heidi Salminen, Jukka Hiltunen, Anne Kanerva, Annamari Heiskanen, Ann Westerholm-Parvinen, Georg Schmidt, Anne Huuskonen, Eero Mustalahti, Christopher Landowski, Markku Saloheimo, Juhani Saarinen, Benjamin Patrick Sommer, Ramon Wahl, Christian Ostermeier, Bernhard Helk
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Patent number: 9567596Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: GrantFiled: January 4, 2013Date of Patent: February 14, 2017Assignee: Glykos Finland OYInventors: Christopher Landowski, Anne Huuskonen, Juhani Saarinen, Ann Westerholm-Parvinen, Anne Kanerva, Jari Natunen, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Markku Saloheimo
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Publication number: 20170002095Abstract: The present invention describes glycans, which are specifically expressed by certain cancer cells, tumours and other malignant tissues. The present invention describes methods to detect cancer specific glycans as well as methods for the production of reagents binding to said glycans. The invention is also directed to the use of said glycans and reagents binding to them for the diagnostics of cancer and malignancies. Furthermore, the invention is directed to the use of said glycans and reagents binding to them for the treatment of cancer and malignancies. Moreover, the present invention comprises efficient methods to differentiate between malignant and benign tumors by analyzing glycan structures.Type: ApplicationFiled: September 14, 2016Publication date: January 5, 2017Applicant: Glykos Finland OyInventors: Tero SATOMAA, Jari NATUNEN, Annamari HEISKANEN, Anne OLONEN, Juhani SAARINEN, Noora SALOVUORI, Jari HELIN
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Patent number: 9464137Abstract: The invention relates to a composition comprising a glycoprotein comprising the Fc domain of an antibody, or a fragment thereof, comprising an Asn (asparagine) residue and an oligosaccharide structure attached thereto, wherein said oligosaccharide structure has a structure according to formula I; and wherein at least 20% of the oligosaccharide structures attached to glycoprotein in the composition consist of oligosaccharide structures according to formula (I).Type: GrantFiled: December 13, 2012Date of Patent: October 11, 2016Assignee: Glykos Finland OyInventors: Tero Satomaa, Juhani Saarinen, Jari Natunen, Anja Vilkman, Heidi Virtanen, Jukka Hiltunen
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Carbohydrate profile compositions from human cells and methods for analysis and modification thereof
Patent number: 9410126Abstract: The invention describes methods for production of novel composition of glycans, glycomes, from human multipotent stem cells. The invention is further directed to methods for modifying the glycomes and analysis of the glycomes and the modified glycomes. Furthermore the invention is directed to stem cells carrying the modified glycomes on their surfaces.Type: GrantFiled: March 6, 2014Date of Patent: August 9, 2016Assignee: GLYKOS FINLAND OYInventors: Tero Satomaa, Jari Natunen, Jarmo Laine, Annamari Heiskanen, Maria Blomqvist, Anne Olonen, Juhani Saarinen, Taina Jaatinen, Ulla Impola, Milla Mikkola, Heidi Anderson -
Patent number: 9320291Abstract: The present invention is directed to a method of production of an immunostimulatory composition having a saccharide fraction, the method including the steps of hydrolyzes of yeast cells and recovering the soluble fraction. The saccharide fraction having composition thus obtained may be incorporated as a food or beverage component or be used as a pharmaceutical for treatment of specific conditions.Type: GrantFiled: December 18, 2009Date of Patent: April 26, 2016Assignee: GLYKOS FINLAND OYInventors: Juhani Saarinen, Ritva Niemela, Jari Helin, Jari Natunen, Jukka Hiltunen
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Publication number: 20160068818Abstract: The invention relates to a method for generating induced pluripotent stem cells, wherein the method comprises: step a) of inducing non-pluripotent cells to produce a mixture comprising induced pluripotent stem cells and non-pluripotent cells; step b) of contacting the mixture comprising induced pluripotent stem cells and non-pluripotent cells obtainable from step a) with a binding agent, wherein the binding agent is capable of binding an epitope consisting of the non-reducing terminal saccharide structure according to formula (Fuc?1-2)aGal?1-3HexNAc?, wherein a=0 or 1 and Hex is either Glc or Gal; and step c) of selecting an induced pluripotent stem cell bound by the binding agent.Type: ApplicationFiled: April 16, 2014Publication date: March 10, 2016Applicant: Glykos Finland OyInventors: Milla MIKKOLA, Timo OTONKOSKI, Heli Mononen, Tero SATOMAA, Juhani SAARINEN
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Publication number: 20150210777Abstract: The invention relates to a pharmaceutical composition comprising a glycoprotein comprising the Fc domain of an antibody, or a fragment thereof, comprising an Asn (asparagine) residue and an oligosaccharide structure attached thereto, wherein said oligosaccharide structure has a structure according to formula I, wherein at least 10% of the oligosaccharide structures attached to glycoproteins in the composition consist of oligosaccharide structures according to formula I.Type: ApplicationFiled: December 13, 2012Publication date: July 30, 2015Inventors: Tero Satomaa, Juhani Saarinen, Jari Natunen, Anja Vilkman, Heidi Virtanen, Jukka Hiltunen
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Publication number: 20150191544Abstract: The invention relates to a composition comprising a glycoprotein comprising the Fc domain of an antibody, or a fragment thereof, comprising an Asn (asparagine) residue and an oligosaccharide structure attached thereto, wherein said oligosaccharide structure has a structure according to formula I; and wherein at least 20% of the oligosaccharide structures attached to glycoprotein in the composition consist of oligosaccharide structures according to formula (I).Type: ApplicationFiled: December 13, 2012Publication date: July 9, 2015Inventors: Tero Satomaa, Juhani Saarinen, Jari Natunen, Anja Vilkman, Heidi Virtanen, Jukka Hiltunen
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Publication number: 20150176044Abstract: Described herein are compositions including filamentous fungal cells, such as Trichoderma fungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example, Trichoderma fungal cells, as the expression system.Type: ApplicationFiled: May 23, 2013Publication date: June 25, 2015Inventors: Jari Natunen, Ann Westerholm-Parvinen, Hanna Salo, Jussi Joensuu, Heli Viskari, Christopher Landowski, Anne Huuskonen, Anne Kanerva, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Juhani Saarinen, Markku Saloheimo
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Publication number: 20150140576Abstract: The present invention describes glycans, which are specifically expressed by certain cancer cells, tumours and other malignant tissues. The present invention describes methods to detect cancer specific glycans as well as methods for the production of reagents binding to said glycans. The invention is also directed to the use of said glycans and reagents binding to them for the diagnostics of cancer and malignancies. Furthermore, the invention is directed to the use of said glycans and reagents binding to them for the treatment of cancer and malignancies. Moreover, the present invention comprises efficient methods to differentiate between malignant and benign tumors by analyzing glycan structures.Type: ApplicationFiled: October 24, 2014Publication date: May 21, 2015Applicant: GLYKOS FINLAND OYInventors: Tero SATOMAA, Jari NATUNEN, Annamari HEISKANEN, Anne OLONEN, Juhani SAARINEN, Noora SALOVUORI, Jari HELIN
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Publication number: 20150072419Abstract: The invention describes methods for production of novel composition of glycans, glycomes, from human multipotent stem cells. The invention is further directed to methods for modifying the glycomes and analysis of the glycomes and the modified glycomes. Furthermore the invention is directed to stem cells carrying the modified glycomes on their surfaces.Type: ApplicationFiled: March 6, 2014Publication date: March 12, 2015Inventors: Tero SATOMAA, Jari NATUNEN, Jarmo LAINE, Annamari HEISKANEN, Maria BLOMQVIST, Anne OLONEN, Juhani SAARINEN, Taina JAATINEN, Ulla IMPOLA, Milla MIKKOLA, Heidi ANDERSON
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Publication number: 20140370546Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: ApplicationFiled: January 4, 2013Publication date: December 18, 2014Inventors: Christopher Landowski, Anne Huuskonen, Juhani Saarinen, Ann Westerholm-Parvinen, Anne Kanerva, Jari Natunen, Anna-Liisa Hänninen, Noora Salovuori, Merja Pentillä, Markku Saloheimo
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Patent number: 8759005Abstract: The present invention reveals novel methods for producing novel carbohydrate compositions, glycomes, from animal tissues. The tissue substrate materials can be total tissue samples and fractionated tissue parts, or artificial models of tissues such as cultivated cell lines. The invention is further directed to the compositions and compositions produced by the methods according to the invention. The invention further represent methods for analysis of the glycomes, especially mass spectrometric methods.Type: GrantFiled: July 11, 2006Date of Patent: June 24, 2014Assignee: Glykos Finland OyInventors: Tero Satomaa, Jari Natunen, Annamari Heiskanen, Maria Blomqvist, Anne Olonen, Juhani Saarinen
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Publication number: 20140173016Abstract: A method for writing data to a memory device comprising a first and a second memory device the first memory device comprises data blocks numbered with block numbers and the second memory device comprises at least one reference calculated from a data block digest and its physical block number. The invention comprises at least the steps: calculating the digest from at least part of the data block content, receiving at least one physical block number, to which the data block contents in the first memory device is stored, encrypting the data block content, storing the data block content to the first memory device to the position pointed by the physical block number, and storing or issuing a command to save the digest, or a number derived from it, and at least one said physical block number to the second memory device. A system, computer program, and server are also presented.Type: ApplicationFiled: September 18, 2013Publication date: June 19, 2014Applicant: Envault Corporation OYInventors: Markku-Juhani Saarinen, Ville Ollikainen
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Patent number: 8703488Abstract: The invention relates to a method for culturing human embryonic stem cells (hESCs) and/or induced pluripotent stem (iPS) cells on a lectin. The invention relates also to the use of a lectin in a method for culturing human embryonic stem cells (hESCs) and/or induced pluripotent stem (iPS) celts and a culture medium composition containing a lectin attached on the culturing plates.Type: GrantFiled: July 13, 2009Date of Patent: April 22, 2014Assignees: Suomen Punainen Risti Veripalvelu, Glykos Finland OyInventors: Ulla Impola, Minna Tiittanen, Milla Mikkola, Jukka Partanen, Jari Natunen, Tero Satomaa, Juhani Saarinen
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Carbohydrate profile compositions from human cells and methods for analysis and modification thereof
Patent number: 8691774Abstract: The invention describes methods for production of novel composition of glycans, glycomes, from human multipotent stem cells. The invention is further directed to methods for modifying the glycomes and analysis of the glycomes and the modified glycomes. Furthermore the invention is directed to stem cells carrying the modified glycomes on their surfaces.Type: GrantFiled: July 11, 2006Date of Patent: April 8, 2014Assignee: Glykos Finland OyInventors: Tero Satomaa, Jari Natunen, Jarmo Laine, Annamari Heiskanen, Maria Blomqvist, Anne Olonen, Juhani Saarinen, Taina Jaatinen, Ulla Impola, Milla Mikkola, Heidi Anderson -
Patent number: 8555088Abstract: The invention concerns a method for writing data to a memory device arrangement comprising a first and a second memory device in which the first memory device comprises data blocks numbered with block numbers and the second memory device comprises at least one reference calculated from a data block digest and its physical block number. The invention is characterized in that it comprises the following steps: calculating the digest from at least part of the data block content, receiving at least one physical block number, to which the data block contents in the first memory device is stored, encrypting the data block content, storing the data block content to the first memory device to the position pointed by the physical block number, and storing or issuing a command to save the digest, or a number derived from it, and at least one said physical block number to the second memory device. Also a system, a computer program and server computer in accordance to the invention are presented.Type: GrantFiled: March 16, 2009Date of Patent: October 8, 2013Assignee: Envault Corporation OyInventors: Markku-Juhani Saarinen, Ville Ollikainen