Patents by Inventor Leonardo De Maria

Leonardo De Maria has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20160017296
    Abstract: The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: October 5, 2015
    Publication date: January 21, 2016
    Inventors: Monica Tassone, Leonardo De Maria
  • Patent number: 9173421
    Abstract: The present invention relates to a method for producing phytase variants has at least 70% identity to a phytase derived from Buttiauxella and comprises at least one additional disulfide bond as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
    Type: Grant
    Filed: March 25, 2011
    Date of Patent: November 3, 2015
    Assignee: Novozymes A/S
    Inventors: Leonardo De Maria, Lars Kobberoee Skov, Michael Skjoet
  • Patent number: 9163220
    Abstract: The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: April 1, 2014
    Date of Patent: October 20, 2015
    Assignees: Novozymes A/S, Novozymes, Inc.
    Inventors: Monica Tassone, Leonardo De Maria
  • Patent number: 9131711
    Abstract: The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.
    Type: Grant
    Filed: May 23, 2014
    Date of Patent: September 15, 2015
    Assignee: Novozymes A/S
    Inventors: Leonardo De Maria, Carsten Andersen, Lars Lehmann Hyllling Christensen, Soren Flensted Lassen, Peter Rahbek Ostergaard
  • Publication number: 20150240425
    Abstract: The present invention relates to variants of a parent laccase. The present invention also relates to polynucleotides encoding the variant laccases and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.
    Type: Application
    Filed: May 11, 2015
    Publication date: August 27, 2015
    Inventors: Lars Henrik Oestergaard, Leonardo De Maria, Miguel Duarte Toscano
  • Patent number: 9109211
    Abstract: The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: July 19, 2013
    Date of Patent: August 18, 2015
    Assignee: Novozymes A/S
    Inventors: Carsten Sjoeholm, Soeren Flensted Lassen, Lars Kobberoee Skov, Leonardo De Maria
  • Publication number: 20150191755
    Abstract: The present invention relates to the use of glycosyl hydrolase family 61 polypeptides in the presence of cutinases for polyester textile manufacture as well as a textile composition comprising glycosyl hydrolase family 61 polypeptides and cutinases.
    Type: Application
    Filed: July 18, 2013
    Publication date: July 9, 2015
    Inventors: Weijian Lai, Ting Sun, Leonardo De Maria
  • Patent number: 9057040
    Abstract: The present invention relates to variants of a parent laccase. The present invention also relates to polynucleotides encoding the variant laccases and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.
    Type: Grant
    Filed: April 16, 2009
    Date of Patent: June 16, 2015
    Assignee: NOVOZYMES A/S
    Inventors: Lars Henrik Oestergaard, Leonardo De Maria, Miguel Duarte Toscano
  • Publication number: 20150159149
    Abstract: The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
    Type: Application
    Filed: February 20, 2015
    Publication date: June 11, 2015
    Inventors: Lars Kobberoee Skov, Leonardo De Maria, Tomoko Matsui
  • Publication number: 20150152397
    Abstract: The present invention relates to isolated polypeptides having organophosphorous hydrolase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: May 30, 2013
    Publication date: June 4, 2015
    Inventors: Bjarne Gram Hansen, Steffen Danielsen, Lars Kobberoee Skov, Leonardo De Maria, Julie Bille Rannes
  • Publication number: 20150024084
    Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
    Type: Application
    Filed: October 2, 2014
    Publication date: January 22, 2015
    Inventors: Leonardo De Maria, Carsten Andersen, Lars Kobberoee Skov, Mikael Blom Soerensen
  • Publication number: 20150023945
    Abstract: The present invention is directed to lysozyme variants and nucleotide sequences encoding same. The lysozyme variants have antimicrobial and/or lysozyme activity and comprise an alteration of an amino acid sequence at one or more positions. The present invention is also directed to methods for producing and of using the Opisthocomus hoazin lysozyme and the lysozyme variants of the present invention as antimicrobial agents.
    Type: Application
    Filed: September 29, 2014
    Publication date: January 22, 2015
    Inventors: Mikkel Klausen, Marie Allesen Holm, Leonardo De Maria, Michael Skjoet
  • Patent number: 8877471
    Abstract: The present invention relates to a phytase which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least one alteration as compared to this phytase. These phytase variants have amended, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an amended glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.
    Type: Grant
    Filed: May 1, 2013
    Date of Patent: November 4, 2014
    Assignee: Novozymes A/S
    Inventors: Leonardo De Maria, Carsten Andersen, Lars Kobberoee Skov, Mikael Blom Soerensen
  • Patent number: 8865637
    Abstract: The present invention relates to variant lysozymes. The present invention also relates to polynucleotides encoding the variant lysozymes and to nucleic acid constructs, vectors, and host cells comprising the polynucleotide.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: October 21, 2014
    Assignee: Novozymes Als
    Inventor: Leonardo De Maria
  • Publication number: 20140255550
    Abstract: The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.
    Type: Application
    Filed: May 23, 2014
    Publication date: September 11, 2014
    Applicant: Novozymes A/S
    Inventors: Leonardo De Maria, Carsten Andersen, Lars Lehmann Hyllling Christensen, Soren Flensted Lassen, Peter Rahbek Ostergaard
  • Publication number: 20140255997
    Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: October 11, 2012
    Publication date: September 11, 2014
    Inventors: Esben Peter Friis, Leonardo De Maria, Jesper Vind, Thomas A. Poulsen, Allan Svendsen, Steffen Danielsen, Rolf T. Lenhard, Henrik Friis-Madsen, Lars K. Skov
  • Publication number: 20140212938
    Abstract: The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: April 1, 2014
    Publication date: July 31, 2014
    Applicants: Novozymes A/S, Novozymes, Inc.
    Inventors: Monica Tassone, Leonardo De Maria
  • Patent number: 8772011
    Abstract: The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.
    Type: Grant
    Filed: January 10, 2013
    Date of Patent: July 8, 2014
    Assignee: Novozymes A/S
    Inventors: Leonardo De Maria, Carsten Andersen, Lars Lehmann Hylling Christensen, Soren Flensted Lassen, Peter Rahbek Ostergaard
  • Patent number: 8728788
    Abstract: The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: September 25, 2012
    Date of Patent: May 20, 2014
    Assignees: Novozymes A/S, Novozymes, Inc.
    Inventors: Monica Tassone, Leonardo De Maria
  • Patent number: 8697063
    Abstract: The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: April 15, 2014
    Assignee: Novozymes A/S
    Inventors: Carsten Sjoeholm, Soeren Flensted Lassen, Lars Kobberoee Skov, Leonardo De Maria