Abstract: The disclosure in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the disclosure relates to gene transfer methods using the recombinant adeno-associated viruses. In some aspects, the disclosure relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

Abstract: An insulin aspart derivative and a preparation method therefor are provided. The derivative has a fusion protein of a green fluorescent protein folding unit and an insulin aspart precursor or an active fragment thereof. The expression level of the fusion protein is significantly increased, and the insulin aspart precursor protein in the fusion protein is folded correctly and has a biological activity. In addition, the green fluorescent protein folding unit in the fusion protein can be digested into small fragments by a protease, which has a large molecular weight difference compared to the target protein and is thus easy to separate. Further provided is a method for preparing the insulin aspart and an intermediate by means of using the fusion protein.

Abstract: A method for reducing blasting vibration of tunneling includes the steps of: dividing the cutting holes into several groups according to different cutting methods; conducting layout and test blasting on one group of cutting holes; testing blasting parameters for the layout and test blasting; calculating an interval t; conducting layout and test blasting on at least two groups of cutting holes again; testing blasting parameters for the layout and test blasting of each group for the second time; taking the minimum interval t of each group as the interval for formal blasting of the remaining groups of cutting holes. The use of the characteristics of the wave in the invention can superpose the peaks and troughs of the vibration wave, and further cancel out mutually; reduce the peaks of the blasting vibration and fill the troughs of the blasting vibration.

Abstract: Embodiments of the present application relate to the field of communication and disclose a time synchronization method, an electronic device, a server and a storage medium. In the present application, an air interface message transmitted by a base station is received, and parsing out synchronization time information and leap predication information from the air interface message are parsed out; and it performs time synchronization with the base station according to the synchronization time information and the leap predication information.

Abstract: The disclosure provides a border touch module, including a cover plate, a shielding layer, a first adhesive layer, a sensing electrode layer, a second adhesive layer, an opaque adhesive, and a backlight layer. The shielding layer is disposed below the cover plate. The first adhesive layer is disposed below the shielding layer. The sensing electrode layer is disposed below the first adhesive layer. The second adhesive layer is disposed below the sensing electrode layer. The opaque adhesive is disposed below the sensing electrode layer. The backlight layer is disposed below the opaque adhesive and is in the same plane as the second adhesive layer. The shielding layer has a backlight pattern, and the opaque adhesive has a light source hole. When the backlight layer is in the state of driving backlight, the light illuminates the backlight pattern of the shielding layer through the light source hole.

Abstract: The present disclosure provides a vibration exciting system and an apparatus for testing an aero-engine rotor, relating to the field of aero-engines, to improve the universality of the vibration exciting system. The vibration exciting system comprises a mounting ring, fixing parts, holders, guide rails, and nozzle assemblies. The mounting ring is configured to be annular; one ends of the fixing parts are fixedly connected to the mounting ring; the holders are slidably mounted at the other ends of the fixing parts, the plurality of holders being arranged circumferentially of the mounting ring; each guide rail connects two adjacent holders, connection positions of the guide rail and the holders being adjustable; and the nozzle assemblies are mounted on the holders. The radius size of a spraying area enclosed by the nozzle assemblies is adjusted by adjusting the positions of the holders on the fixing parts.

Abstract: Aspects of the disclosure relate to compositions and methods for delivering a transgene (e.g., a transgene encoding one or more gene products) to a target cell. The disclosure is based, in part, on adeno-associated virus (AAV) capsid protein variants characterized by tropisms for certain cell types (e.g., neurons, muscle cells, bone cells, heart cells, etc.). In some embodiments, recombinant A A Vs (rAAVs) comprising the capsid protein variants (e.g., AAVv66, SEQ ID NO: 1) are more efficiently packaged than rAAVs having certain wild-type AAV capsid proteins. Methods of delivering an rAAV comprising the AAV capsid protein variants are also described by the disclosure.

Abstract: An insulin degludec derivative and a preparation method therefor are provided. Specifically, a fusion protein has a green fluorescent protein folding unit and an insulin degludec precursor or an active fragment thereof are provided. The fusion protein has a significantly increased expression level, and the insulin degludec precursor protein in the fusion protein is folded correctly, and has biological activity. Moreover, the green fluorescent protein folding unit in the fusion protein can be digested into small fragments by a protease, which have a great difference in molecular weight in comparison to a target protein, and are easy to separate. A method for using the fusion protein to prepare insulin degludec and prepare an intermediate thereof are also provided.

Abstract: Provided is an air cleaner that can take a large amount of air in a large space into a dust collector with good efficiency while being lightweight and having easy maintenance. An air cleaner is provided with a drone and a dust collector. The drone has a main body unit and propellers attached to the tips of frames. The dust collector has electric discharge electrodes and a dust collection electrode. The electric discharge electrodes are connected to a booster unit within a central chamber. The booster unit is electrically connected to a control unit in the main body unit. Electric discharge is formed between the dust collection electrode and the electric discharge electrodes, and dust particles in the air are charged and collected by the dust collection electrode.

Abstract: The disclosure provides a border touch module, including a cover plate, a shielding layer, a first adhesive layer, a sensing electrode layer, a second adhesive layer, an opaque adhesive, and a backlight layer. The shielding layer is disposed below the cover plate. The first adhesive layer is disposed below the shielding layer. The sensing electrode layer is disposed below the first adhesive layer. The second adhesive layer is disposed below the sensing electrode layer. The opaque adhesive is disposed below the sensing electrode layer. The backlight layer is disposed below the opaque adhesive and is in the same plane as the second adhesive layer. The shielding layer has a backlight pattern, and the opaque adhesive has a light source hole. When the backlight layer is in the state of driving backlight, the light illuminates the backlight pattern of the shielding layer through the light source hole.

Abstract: The disclosure in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the disclosure relates to gene transfer methods using the recombinant adeno-associated viruses. In some aspects, the disclosure relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

Abstract: A wetland system has a wetland structure and a wastewater treatment device. The wetland structure has several planting areas surrounded by hoardings, several planting frames arranged in the hoardings, growing substrates provided in the planting frames, and wetland plants growing on the growing substrates. The wastewater treatment device has a bar screen, a regulating tank, a hydrolysis acidification tank, a contact oxidation tank, an MBR membrane tank, and a clean water tank that are in communication with each other in sequence of waterflow and arranged underground. The bar screen is provided with several bars therein, a lift pump is connected between the regulating tank and the hydrolysis acidification tank, a drainage pump is provided between the MBR membrane tank and the clean water tan. The planting area is connected to the bar screen via pipelines, and the clean water tank is connected to the planting area via pipelines.

Abstract: A method of making a semiconductor device includes: providing a substrate; forming an insulating layer on the substrate; forming a first trench in the insulating layer; forming a first semiconductor layer in the first trench; and removing a portion of the insulating layer to expose the first semiconductor layer.

Abstract: This disclosure discloses a multiplayer somatosensory system, method, and apparatus combining virtual and reality, and a medium, which belongs to the field of human-computer interaction. The system includes: a computer device, and a simulation carrier, n simulation firearms and a display apparatus; where the simulation carrier includes a driver's seat and n passenger seats; the display apparatus is configured to display a virtual environment picture and n aiming points provided by the computer device; and the computer device is configured to control a virtual carrier to change a driving direction in the virtual environment; control the virtual carrier to change a driving speed in the virtual environment; and shoot a virtual object aimed at an ith aiming point in the virtual environment in response to a shooting operation on an ith simulation firearm in the n simulation firearms.

Abstract: The present invention discloses a pile head girdling device comprising a track arranged on a pile head and coaxial with the pile head, an electric saw installation base arranged on the track in a sliding mode, an electric saw support rotationally arranged on the electric saw installation base and an electric saw arranged on the electric saw support. The beneficial effects of the present invention are as follows: according to the solution, the electric saw cutting position on the pile head is effectively controlled on the same plane, thereby improving the quality of cutting the pile head; and the electric saw cutting position is guided and limited by means of the track, thereby improving the cutting efficiency and precision.

Abstract: A control method, a control apparatus and a device for preventing pseudo GNSS signal interference, and a storage medium are provided. The method includes: in response to detecting that a received GNSS signal includes a pseudo GNSS signal, restraining a time service system of a communication device from tracking the received GNSS signal; and removing the restraining on the time service system in response to detecting that the pseudo GNSS signal disappears.

Abstract: A touch display device for preventing light leakage is provided, including a display module, a first adhesive layer, a touch sensing film, and at least one protective layer. The display module includes an upper surface, a side surface, and a lower surface. The first adhesive layer is disposed on the upper surface of the display module and is bent and extends along the side surface to the lower surface, and the touch sensing film is disposed on the first adhesive layer. The at least one protective layer is disposed on one side of the touch sensing film relative to the side surface and the lower surface of the display module. At least one of the first adhesive layer disposed on the side surface of the display module or the protective layer is an opaque material, so the thickness of the device can be reduced.

Abstract: The invention discloses a device and a method for accurately fixing an internal waterstop in a lakebed tunnel, comprising two supporting bars arranged in parallel, two transverse clamping bars arranged in parallel between the supporting bars, vertical clamping bars arranged on the transverse clamping bars, wherein the supporting bars, the transverse clamping bars and the vertical clamping bars are mutually perpendicular, at least two vertical clamping bars are arranged on each transverse clamping bar, and the vertical clamping bars are arranged at the inner side of the transverse clamping bars. The beneficial effects of the invention are as follows: With vertical clamping bars that clamp the waterstop, the invention can limit the movement and rotational degree of freedom of the waterstop within in the space, to avoid displacement of the waterstop, thus making the construction more productive.

Abstract: An apparatus, the apparatus comprising at least one processor, and at least one memory including computer program code, the at least one memory and the computer program code configured, with the at least one processor, to cause the apparatus to perform at least the following: based on one or more determined input manner characteristics of user electronic-scribed input, associate the user electronic-scribed input with a function to be performed using the user electronic-scribed input.

Abstract: A touch display device for preventing light leakage is provided, including a display module, a first adhesive layer, a touch sensing film, and at least one protective layer. The display module includes an upper surface, a side surface, and a lower surface. The first adhesive layer is disposed on the upper surface of the display module and is bent and extends along the side surface to the lower surface, and the touch sensing film is disposed on the first adhesive layer. The at least one protective layer is disposed on one side of the touch sensing film relative to the side surface and the lower surface of the display module. At least one of the first adhesive layer disposed on the side surface of the display module or the protective layer is an opaque material, so the thickness of the device can be reduced.