Abstract: A method of manufacturing a mat is provided. The method involves securing a first layer formed of a polymer within a base die, positioning an intermediate cushion layer on top of the first layer, securing a second layer formed of the polymer to the base die, and securing a perimeter of the first layer to a perimeter of the second layer by radio-frequency welding, so as to envelop the intermediate cushion layer within the first and second layers and thereby form the mat. The first layer has a first surface and an opposing second surface in contact with the base die. The intermediate cushion layer has a first surface and an opposing second surface in contact with the first surface of the first layer. The second layer has a first surface and an opposing second surface in contact with the first surface of the intermediate cushion layer.

Abstract: The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

Abstract: The present application relates to a compositions and methods comprising or expressing a HEVAR or MOMO30 protein derived from Momordica balsamina. The HEVAR or MOMO30 protein and/or a nucleic acid encoding the same may be used in methods for treating or preventing viral infections, particularly those caused by coronaviruses, such as SARS-CoV-2.

Abstract: The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

Abstract: The present application relates to a compositions and methods comprising or expressing a HEVAR or MOMO30 protein derived from Momordica balsamina. The HEVAR or MOMO30 protein and/or a nucleic acid encoding the same may be used in methods for treating or preventing viral infections, particularly those caused by coronaviruses, such as SARS-CoV-2.

Abstract: The present application relates to a compositions and methods comprising or expressing a HEVAR or MOMO30 protein derived from Momordica balsamina. The HEVAR or MOMO30 protein and/or a nucleic acid encoding the same may be used in methods for treating or preventing viral infections, particularly those caused by coronaviruses, such as SARS-CoV-2.

Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: The present application relates to a compositions and methods comprising or expressing a MOMO30 protein derived from Momordica balsamina. The MOMO30 protein is about 30 kDa in size, binds coronavirus S protein, is stable after being autoclaved at 120° C. for 30 min, resists proteolytic cleavage by trypsin, exhibits mannose-sensitive binding to coronavirus spike (S) protein, exhibits hemagglutinin and chitinase activity, is capable of activating and stimulating T cell proliferation, is capable of preventing infection by a coronavirus or alleviating symptoms in coronavirus infected patients and comprises the amino acid sequence of SEQ ID NO: 1. The MOMO30 protein and/or a nucleic acid encoding the same may be used in methods for preventing or treating viral infections by coronaviruses and others.

Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: A molten metal conveyor having a receptor plate in contact with molten metal during transport of the molten metal. The receptor plate extends from an entrance where molten metal enters onto the receptor plate to an exit where molten metal exits the receptor plate. The molten metal conveyor has at least one vibrational energy source which supplies vibrational energy directly to the receptor plate in contact with molten metal. A corresponding method for forming a metal product includes providing molten metal onto a molten conveyor; cooling the molten metal by control of a cooling medium flowing through a cooling passage in the or attached to the conveyor; and coupling vibrational energy directly into a receptor plate in contact with the molten metal on the conveyor.

Abstract: The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

Abstract: Compositions and methods for reducing the growth of and/or preventing the formation of a microbial biofilm are disclosed. The composition comprises an antimicrobial SMR peptide comprising an HIV-1 SMRwt peptide and a cell penetrating peptide (CPP) domain. In some embodiments, the composition further comprises one or more other antimicrobial peptides (AMPs), antibiotics, matrix-inhibiting compounds, matrix-disaggregating compounds, quorum sensing inhibitors, or a combination thereof. In other embodiments, the compositions are used for impregnating or coating an article and/or material surface with the composition to render it less prone to microbial infections.

Abstract: A motor vehicle having a vehicle body, an engine compartment (10) housing an engine (12) and a bonnet seal arrangement (30, 34). The bonnet seal arrangement defines an opening (35) and is configured to deflect ram air received at the front of the motor vehicle (2) in use past the opening (35) to define a first air flow path, and draw air from the first air flow path through the opening (35) to define a second air flow path for supplying air to the intake duct orifice (42), the second air flow path being angled in a horizontal plane to the first air flow path.

Abstract: Systems and methods for user generated content authoring are disclosed. One illustrative method disclosure herein includes: receiving a video signal; displaying a user interface associated with haptic authoring; detecting a gesture associated with a haptic effect; determining a haptic effect based in part on the gesture; associating the haptic effect with a location in the video signal; and storing the video signal and the associated haptic effect.

Abstract: A liquid container has a plurality of resilient liquid permeable bodies inserted via an aperture and attached to a mounting by a tether, so as to interrupt the free flow of liquid within the container and prevent noise and vibration resulting from the contained fluid sloshing. A method describes how the anti-slosh device can be installed into a liquid container and how the device can be retrofitted to any liquid container.

Abstract: A method of screening a sample of body fluid obtained from an animal subject for analyte autoantibodies reactive with one or more antigenic molecules selected from pancreatic islet cell antigenic molecules (GAD, 1A2) and insulin, or one or more variants, analogs, derivatives or fragments thereof, and a kit for use in such a method. After addition of the sample one or more complexes comprising [antigenic molecule of first source]-[analyte auto antibody]-[antigenic molecule of second source]. The antigenic molecule of first source is immobilized to a solid phase, the second is labelled.

Abstract: A method of screening a sample of body fluid obtained from an animal subject for analyte autoantibodies reactive with one or more antigenic molecules selected from pancreatic islet cell antigenic molecules (GAD, 1A2) and insulin, or one or more variants, analogs, derivatives or fragments thereof, and a kit for use in such a method. After addition of the sample one or more complexes comprising [antigenic molecule of first source]-[analyte auto antibody]-[antigenic molecule of second source]. The antigenic molecule of first source is immobilized to a solid phase, the second is labeled.

Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: Systems and methods for user generated content authoring are disclosed. One illustrative method disclosure herein includes: receiving a video signal; displaying a user interface associated with haptic authoring; detecting a gesture associated with a haptic effect; determining a haptic effect based in part on the gesture; associating the haptic effect with a location in the video signal; and storing the video signal and the associated haptic effect.