Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

Abstract: Compositions and methods for reducing the growth of and/or preventing the formation of a microbial biofilm are disclosed. The composition comprises an antimicrobial SMR peptide comprising an HIV-1 SMRwt peptide and a cell penetrating peptide (CPP) domain. In some embodiments, the composition further comprises one or more other antimicrobial peptides (AMPs), antibiotics, matrix-inhibiting compounds, matrix-disaggregating compounds, quorum sensing inhibitors, or a combination thereof. In other embodiments, the compositions are used for impregnating or coating an article and/or material surface with the composition to render it less prone to microbial infections.

Abstract: Implementations of a two-piece electrical connector are provided. In some implementations, the two-piece electrical connector may be used to conductively connect two electronic devices (e.g., audio equipment). In some implementations, the two-piece electrical connector may be configured to reduce signal degradation (e.g., skin effect, noise, distortion, etc.) during transmission between connected electronic devices. In some implementations, the two-piece electrical connector may comprise a plug and a jack configured to connect together and thereby complete an electrical circuit. In some implementations, the plug and jack may each include two contacts. In some implementations, each contact is a segment of a foil conductor that is a single unitary piece of material (i.e., there are no breaks or soldered joints). In this way, through the use of foil conductors having a unitary construction, signal degradation is reduced between electronic devices connected together by a two-piece electrical connector.

Abstract: The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

Abstract: Methods are described for determining whether a subject suffering from, or suspected of suffering from, an infectious disease caused by a microbe is infected with a strain of the microbe that is susceptible to an antimicrobial agent, where there exist different strains of the microbe that are resistant to the antimicrobial agent. The methods comprise determining whether nucleic acid of the strain of the microbe infecting the subject comprises wild-type nucleotide sequence at a conserved nucleotide position at which mutation is associated with resistance to the antimicrobial agent in nucleic acid of the different resistant strains. The methods are particularly applicable for determining whether a subject suffering from, or suspected of suffering from, Gonorrhoea is infected with a strain of Neisseria gonorrhoeae that is susceptible to an antimicrobial agent. Kits for use in the methods are described, as well as methods for treatment of infectious disease.

Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: An auxiliary security code called a CVC2a is generated to replace a CVC2 value that is presented on a payment card for verifying card-not-present (CNP) transactions without necessarily generating a replacement card. An enhanced card table/file is maintained by a payment network operator to facilitate transitional scenarios in which the payment cards are reissued or replaced.

Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: A method for applying a sound dampening coating on a surface of a vehicle transmission housing includes providing the transmission housing, and applying a sound dampening material directly on a surface of the transmission housing.

Abstract: Implementations of a two-piece electrical connector are provided. In some implementations, the two-piece electrical connector may be used to conductively connect two electronic devices (e.g., audio equipment). In some implementations, the two-piece electrical connector may be configured to reduce signal degradation (e.g., skin effect, noise, distortion, etc.) during transmission between connected electronic devices. In some implementations, the two-piece electrical connector may comprise a plug and a jack configured to connect together and thereby complete an electrical circuit. In some implementations, the plug and jack may each include two contacts. In some implementations, each contact is a segment of a foil conductor that is a single unitary piece of material (i.e., there are no breaks or soldered joints). In this way, through the use of foil conductors having a unitary construction, signal degradation is reduced between electronic devices connected together by a two-piece electrical connector.

Abstract: A static hinge disabler is provided for preventing the operation of a door hinge. The static hinge disabler includes a first sidewall, a second sidewall, a channel, and a cap. The first sidewall is substantially parallel to the second sidewall. The channel is disposed between the first sidewall and the second sidewall and configured to receive at least a portion of the door hinge therein. The first sidewall and the second sidewall are statically connected such that the channel does not change shape. The first sidewall and the second sidewall therefore prevent the door hinge from operating while the door hinge is disposed within the channel.

Abstract: A liquid container has a plurality of resilient liquid permeable bodies inserted via an aperture and attached to a mounting by a tether, so as to interrupt the free flow of liquid within the container and prevent noise and vibration resulting from the contained fluid sloshing. A method describes how the anti-slosh device can be installed into a liquid container and how the device can be retrofitted to any liquid container.

Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

Abstract: The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

Abstract: The present application discloses a method for treating microbial infection using an antimicrobial composition comprises antimicrobial peptide which contains at least one VGFPV motif.

Abstract: A motor vehicle comprises a vehicle body, an engine compartment housing an engine, a bonnet movably coupled to the body and arranged to provide a closure member for the engine compartment, the bonnet being movable between open and closed conditions to allow access to the engine compartment, and an engine air intake duct having an orifice through which intake air may be drawn into the engine. The vehicle is provided with first and second air flaw paths whereby air may be supplied to the air intake duct. The first flow path comprises an air inlet aperture disposed at a front portion of the vehicle for receiving ram air and an air outlet aperture aft of the air inlet aperture, the air outlet aperture being provided in fluid communication with the air intake duct orifice. The second flow path is provided by a gap between a side edge of the bonnet and the body of the vehicle, whereby ambient air may be drawn through the gap to the air intake duct.

Abstract: Devices and methods for power management during media playback are provided. For example, an electronic device according to an embodiment may include storage, a decoder, an output buffer, and data processing circuitry. The storage may store compressed media data that may be decoded by the decoder. The output buffer may store the decoded media data before the decoded media data is played out. The data processing circuitry configured may measure a fullness of the output buffer and may set an operating frequency of the storage, the decoder, the output buffer, or the data processing circuitry, or a combination thereof, depending on a format of the compressed media data and the fullness of the output buffer.

Abstract: A method of screening a sample of body fluid obtained from an animal subject for analyte autoantibodies reactive with one or more antigenic molecules selected from pancreatic islet cell antigenic molecules (GAD, 1A2) and insulin, or one or more variants, analogues, derivatives or fragments thereof, and a kit for use in such a method. After addition of the sample one or more complexes comprising [antigenic molecule of first source]-[analyte auto antibody]-[antigenic molecule of second source]. The antigenic molecule of first source is immobilised to a solid phase, the second is labelled.