Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and insert-ing the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

Abstract: Provided is a method for detecting a target molecule. The method includes providing a chip, the chip including a nanopore in a membrane that is disposed adjacent to or in proximity to a sensing electrode. A nucleic acid molecule is then directed through the nanopore, the nucleic acid molecule being associated with a reporter molecule. The nucleic acid molecule also includes an address region and a probe region, the reporter molecule being associated with the nucleic acid molecule at the probe region. The reporter molecule is also coupled to a target molecule. While the nucleic acid molecule is directed through the nanopore, the address region can be sequenced to determine a nucleic acid sequence of the address region. The target molecule can then be identified, with the aid of a computer processor, based upon the nucleic acid sequence of the address region.

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

Abstract: The present invention provides new processes for the preparation of unsubstituted and substituted 4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione compounds which are useful, for example, for preventing or treating diseases or conditions related to an abnormally high level or activity of TNF-?. The invention can provide improved and/or efficient processes for the commercial production of unsubstituted and substituted 4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione compounds, including, but not limited to, unsubstituted 4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione.

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

Abstract: A method is disclosed, as well as systems, performed by one or more processors, for interacting with data in a data repository. The method comprises receiving, in a data catalogue environment, a search request relating to one or more items in the data repository and determining an object type associated with the one or more items. Other operations comprise loading an object template in dependence on the determined object type, populating the template with data from the data repository in dependence on the search request to create an object view, and displaying the object view within the data catalogue environment. The data repository comprises a plurality of joined datasets, and wherein the object view comprises one or more links to items in a joined dataset.

Abstract: This disclosure provides systems and methods for attaching nanopore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disclosed tagged nucleotides.

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

Abstract: Examples of a multiple-mask multiple-exposure lithographic technique and suitable masks are provided herein. In some examples, a photomask includes a die area and a stitching region disposed adjacent to the die area and along a boundary of the photomask. The stitching region includes a mask feature for forming an integrated circuit feature and an alignment mark for in-chip overlay measurement.

Abstract: An electronic device controller assembly for ergonomic dual-handed control of the electronic device includes a housing. At least one circuit board is positioned in the housing. The at least one circuit board is configured to selectively and operationally couple to the electronic device. Each of a pair of sticks is pivotally coupled to and extends between the upper face of the housing and a bottom of a respective plate. Each plate is configured to position a respective hand of a user. A plurality of first buttons is coupled to the plates. The sticks and the first buttons are operationally coupled to the at least one circuit board. Each first button is configured to be depressed to selectively control a respective function of the electronic device. Each plate is positioned to compel an associated stick to pivot relative to the housing to signal a directional movement.

Abstract: A lead anchor includes a body defining a lead lumen having a first opening and a second opening through which a lead can pass. The body further defines a transverse lumen that intersects the lead lumen. An exterior member is disposed around at least a portion of the body. The exterior member is formed of a biocompatible material. A fastener anchors the lead to the body through the transverse lumen by deforming a portion of the lead. The transverse lumen is configured and arranged to receive the fastener. At least at least two suture tabs extend from the exterior member and are configured and arranged for receiving a suture to suture the lead anchor to patient tissue.

Abstract: A system for processing sample entities includes a chamber including a surface having an array of measurement regions, wherein at least one measurement region comprises a first set of one or more electrodes and a second set of one or more electrodes, wherein the first set of electrodes is configured to measure a first characteristic of a sample entity when the sample entity is traversing the first set of electrodes, and wherein the second set of electrodes is configured to selectively retain the sample entity in the at least one measurement region based at least in part on the measured first characteristic and/or measure a second characteristic of the sample entity.

Abstract: This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.

Abstract: Examples disclosed herein provide a hinge assembly for a computing device. One example hinge assembly includes a first bracket attached to a first member of the computing device and a second bracket attached to a second member of the computing device. The hinge assembly includes a hinge pivotally connecting the first bracket to the second bracket along a first axis, and a first gear rotatably connected to the first bracket along the first axis. The hinge assembly further includes a set of gears, each gear from the set of gears rotatably connected to different axes parallel to the first axis. As an example, the set of gears is to provide friction against the first gear to provide a level of resistance to a torque provided when the first bracket is to be moved along the first axis.

Abstract: This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to compositions, methods, and systems for sequencing nucleic acids using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

Abstract: An all in the head surface cleaning apparatus includes a surface cleaning head having a lower surface having a dirty air inlet. The surface cleaning head may include a brush motor drivingly connected to a movable brushing member having a brush motor axis. The surface cleaning head may include a cyclone comprising a cyclone chamber having a longitudinal cyclone axis. The surface cleaning head may include a suction motor having a first end, a second end and a suction motor axis extending between the first and second ends. The surface cleaning head may include a bleed valve having an inlet end, an outlet end and a body extending between the inlet and outlet ends. The body may have a bleed valve axis. The bleed valve axis may be generally parallel to at least one of the cyclone chamber axis, the suction motor axis and the brush motor axis.

Abstract: The present invention provides new processes for the preparation of unsubstituted and substituted 4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione compounds which are useful, for example, for preventing or treating diseases or conditions related to an abnormally high level or activity of TNF-?. The invention can provide improved and/or efficient processes for the commercial production of unsubstituted and substituted 4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione compounds, including, but not limited to, unsubstituted 4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione.

Abstract: An all in the head surface cleaning apparatus may have a surface cleaning head including a front end, a rear end, first and second laterally opposed sidewalls. The surface cleaning head may include a cyclone assembly comprising a cyclone chamber and a dirt collection chamber. The cyclone chamber may have a longitudinal cyclone axis, a cyclone assembly air inlet port and a cyclone assembly air outlet port. The cyclone assembly may be moveable from a cleaning position to a cyclone assembly removal position. The surface cleaning head may include a biasing member biasing the cyclone assembly away from the cleaning position, and a suction motor having a suction motor air inlet and a suction motor axis. The apparatus may include an upper portion moveably mounted to the surface cleaning head between a storage position and a floor cleaning position, the upper portion comprising a drive handle.

Abstract: A lead anchor includes a body defining a lead lumen having a first opening and a second opening through which a lead can pass. The body further defines a transverse lumen that intersects the lead lumen. An exterior member is disposed around at least a portion of the body. The exterior member is formed of a biocompatible material. A fastener anchors the lead to the body through the transverse lumen by deforming a portion of the lead. The transverse lumen is configured and arranged to receive the fastener. At least one suture element is defined by the exterior member and is configured and arranged for receiving a suture to suture the lead anchor to patient tissue.

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.